scholarly journals An Immunosensor Based on Au-Ag Bimetallic NPs Patterned on a Thermal Resistant Flexible Polymer Substrate for In-Vitro Protein Detection

Polymers ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 1257 ◽  
Author(s):  
Wang ◽  
Wei ◽  
Tong ◽  
He ◽  
Bai ◽  
...  
Keyword(s):  
Protein Detection ◽  
Polymer Substrate ◽  
Smart Devices ◽  
Ag Nps ◽  
Au Nps ◽  
Flexible Polymer ◽  
Gold Silver ◽  
Flexible Polymer Substrate ◽  
Vitro Protein

Nanosensors based on flexible polymers have emerged as powerful tools for next generation smart devices in the recent years. Here, we report a facile protocol to fabricate an immunosensor supported by a thermally resistant flexible polymer substrate (polyarylene ether nitrile, PEN). The immunosensor is a localized surface plasmon resonance (LSPR) optical sensor for in-vitro protein detection based on anti-body coated gold-silver bimetallic nanoparticles (Au-Ag NPs) immobilized on a PEN substrate. Plasmonic spectroscopy and morphological characterization show that the Au-Ag NPs essentially exhibit a more uniform size distribution and higher quality factors than those from single-component Au NPs. Furthermore, it should be noted that the robust PEN substrate in this nanosensor acts a flexible substrate to support Au-Ag NPs and immobilize the nanoparticles via quick thermal annealing at 290 °C. Thanks to these merits, a prostate-specific antigen (PSA) concentration as low as 1 ng/mL can be specifically discriminated via the prepared PEN/Au-Au NPs, which confirms that the protocol reported in this work can be readily adapted for the construction of various flexible immunosensors for different applications.

scholarly journals Green approach in gold, silver and selenium nanoparticles using coffee bean extract

2020 ◽  
Vol 5 (1) ◽  
pp. 761-767
Author(s):  
Reiyhaneh Abbasian ◽  
Hoda Jafarizadeh-Malmiri
Keyword(s):  
Spherical Shape ◽  
Coffee Bean ◽  
Hydrothermal Conditions ◽  
Ag Nps ◽  
Au Nps ◽  
Stabilizing Agents ◽  
E Coli ◽  
Green Approach ◽  
Gold Silver ◽  
Ft Ir

AbstractGreen fabrication of metal nanoparticles (NPs), using natural reducing and stabilizing agents existed in plants and their derivatives, due to their unique properties, has gained more attention. The present study focuses on the synthesis of gold (Au), silver (Ag) and selenium (Se) NPs using coffee bean extract under hydrothermal conditions (1.5 atm and 121°C, for 15 min). Coffee bean extract obtained in 2 h processing using Clevenger apparatus and Fourier transform-infrared (FT-IR) spectroscopy indicated five highlighted peaks, namely, hydroxyl, amide, aromatic, alkane and ring groups. Dynamic light scattering analysis revealed that among three different NPs formed, fabricated Ag NPs had small particle size (153 nm) and high zeta potential value (16.8 mV). However, synthesized Au NPs had minimum polydispersity index (0.312). Results also indicated that fabricated Au, Se and Ag NPs had low antioxidant activity with values of 9.1, 8.9 and 8.7%, respectively. Morphological and antibacterial activity assessments, demonstrated that synthesized Ag, Au and Se NPs had spherical shape and high bactericidal activity against E. coli and S. aurous. Obtained results indicated that the synthesized NPs, can be utilized in various areas.

scholarly journals Combining in vitro protein detection and in vivo antibody detection identifies potential vaccine targets against Staphylococcus aureus during osteomyelitis

2016 ◽  
Vol 206 (1) ◽  
pp. 11-22 ◽  
Author(s):  
P. Martijn den Reijer ◽  
Marjan Sandker ◽  
Susan V. Snijders ◽  
Mehri Tavakol ◽  
Antoni P. A. Hendrickx ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Protein Detection ◽  
Antibody Detection ◽  
Potential Vaccine ◽  
Vitro Protein

scholarly journals Marine Bacterial Exopolymers-Mediated Green Synthesis of Noble Metal Nanoparticles with Antimicrobial Properties

Polymers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1157 ◽  
Author(s):  
Angela Scala ◽  
Anna Piperno ◽  
Alexandru Hada ◽  
Simion Astilean ◽  
Adriana Vulpoi ◽  
...  
Keyword(s):  
Hydrothermal Vents ◽  
Thermophilic Bacteria ◽  
Silver Chloride ◽  
Antimicrobial Properties ◽  
Biological Evaluation ◽  
Noble Metal Nanoparticles ◽  
Ag Nps ◽  
Au Nps ◽  
Minimum Fungicidal Concentration ◽  
Gold Silver

A straightforward and green method for the synthesis of gold, silver, and silver chloride nanoparticles (Au NPs and Ag/AgCl NPs) was developed using three different microbial exopolymers (EP) as reducing and stabilizing agents. The exopolysaccharides EPS B3-15 and EPS T14 and the poly-γ-glutamic acid γ-PGA-APA were produced by thermophilic bacteria isolated from shallow hydrothermal vents off the Eolian Islands (Italy) in the Mediterranean Sea. The production of metal NPs was monitored by UV−Vis measurements by the typical plasmon resonance absorption peak and their antimicrobial activity towards Gram-positive and Gram- negative bacteria (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa), as well as fungi (Candida albicans) was investigated. The biological evaluation showed no activity for EP-Au NPs, except against E. coli, whereas EP-Ag NPs exhibited a broad-spectrum of activity. The chemical composition, morphology, and size of EP-Ag NPs were investigated by UV–Vis, zeta potential (ζ), dynamic light scattering (DLS) measurements and transmission electron microscopy (TEM). The best antimicrobial results were obtained for EPS B3-15-Ag NPs and EPS T14-Ag NPs (Minimum Inhibitory Concentration, MIC: 9.37–45 µg/mL; Minimum Bactericidal Concentration/Minimum Fungicidal Concentration, MBC/MFC: 11.25–75 µg/mL).

scholarly journals Paintable temperature-responsive cholesteric liquid crystal reflectors encapsulated on a single flexible polymer substrate

2019 ◽  
Vol 7 (24) ◽  
pp. 7395-7398 ◽  
Author(s):  
Hitesh Khandelwal ◽  
Ellen P. A. van Heeswijk ◽  
Albert P. H. J. Schenning ◽  
Michael G. Debije
Keyword(s):  
Liquid Crystal ◽  
Cholesteric Liquid Crystal ◽  
Polymer Substrate ◽  
Polymer Layers ◽  
Temperature Responsive ◽  
Flexible Polymer ◽  
Protective Polymer ◽  
Flexible Polymer Substrate

Temperature-responsive photonic cholesteric liquid crystal reflectors deposited on flexible single substrates, encapsulated by protective polymer layers generated by photo-enforced stratification.

Bendable fuel cells: on-chip fuel cell on a flexible polymer substrate

2009 ◽  
Vol 2 (10) ◽  
pp. 1074 ◽  
Author(s):  
Satoshi Tominaka ◽  
Hiroshi Nishizeko ◽  
Jun Mizuno ◽  
Tetsuya Osaka
Keyword(s):  
Fuel Cells ◽  
Fuel Cell ◽  
Polymer Substrate ◽  
Flexible Polymer ◽  
On Chip ◽  
Flexible Polymer Substrate
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