scholarly journals Recent Advances in Engineered Stem Cell-Derived Cell Sheets for Tissue Regeneration

Polymers ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 209 ◽  
Author(s):  
Hyunbum Kim ◽  
Yunhye Kim ◽  
Jihyun Park ◽  
Nathaniel Hwang ◽  
Yun Lee ◽  
...  
Keyword(s):  
Stem Cell ◽  
Tissue Regeneration ◽  
Proteolytic Enzymes ◽  
Intact Cell ◽  
Cell Sheet ◽  
Intercellular Junctions ◽  
Surface Proteins ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Cell Based Therapy

The substantial progress made in the field of stem cell-based therapy has shown its significant potential applications for the regeneration of defective tissues and organs. Although previous studies have yielded promising results, several limitations remain and should be overcome for translating stem cell-based therapies to clinics. As a possible solution to current bottlenecks, cell sheet engineering (CSE) is an efficient scaffold-free method for harvesting intact cell sheets without the use of proteolytic enzymes, and may be able to accelerate the adoption of stem cell-based treatments for damaged tissues and organs regeneration. CSE uses a temperature-responsive polymer-immobilized surface to form unique, scaffold-free cell sheets composed of one or more cell layers maintained with important intercellular junctions, cell-secreted extracellular matrices, and other important cell surface proteins, which can be achieved by changing the surrounding temperature. These three-dimensional cell sheet-based tissues can be designed for use in clinical applications to target-specific tissue regeneration. This review will highlight the principles, progress, and clinical relevance of current approaches in the cell sheet-based technology, focusing on stem cell-based therapies for bone, periodontal, skin, and vascularized muscles.

scholarly journals Recent Advances in ROS-Responsive Cell Sheet Techniques for Tissue Engineering

2019 ◽  
Vol 20 (22) ◽  
pp. 5656 ◽  
Author(s):  
Min-Ah Koo ◽  
Mi Hee Lee ◽  
Jong-Chul Park
Keyword(s):  
Tissue Engineering ◽  
Cell Sheet ◽  
Cell Detachment ◽  
Oxygen Species ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
New Approach ◽  
Responsive Systems ◽  
Cell Based Therapy ◽  
Responsive Cell

Cell sheet engineering has evolved rapidly in recent years as a new approach for cell-based therapy. Cell sheet harvest technology is important for producing viable, transplantable cell sheets and applying them to tissue engineering. To date, most cell sheet studies use thermo-responsive systems to detach cell sheets. However, other approaches have been reported. This review provides the progress in cell sheet detachment techniques, particularly reactive oxygen species (ROS)-responsive strategies. Therefore, we present a comprehensive introduction to ROS, their application in regenerative medicine, and considerations on how to use ROS in cell detachment. The review also discusses current limitations and challenges for clarifying the mechanism of the ROS-responsive cell sheet detachment.

Thermo-Intelligent Surfaces for Cell Culture

2006 ◽  
Vol 53 ◽  
pp. 70-73
Author(s):  
Teruo Okano
Keyword(s):  
Intact Cell ◽  
Single Cells ◽  
Cell Sheet ◽  
Cell Junctions ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Responsive Polymers ◽  
Temperature Responsive ◽  
Biodegradable Scaffolds ◽  
Tissue Reconstruction

In order to avoid several complications resulted from biodegradable scaffolds or single cell injection, we have developed “cell sheet engineering”. Our concept is novel tissue reconstruction not from single cells but from cell sheets. In order to prepare cell sheets, temperature-responsive culture dishes are utilized. Since temperature-responsive polymers are covalently grafted on the dishes, various types of cells adhere and proliferate on them at 37°C, but are spontaneously detached only by reducing temperature below 32°C without any need for proteolytic enzyme. All the confluent cells are noninvasively harvested as a single contiguous cell sheets with intact cell-cell junctions and deposited extracellular matrix. We have utilized these harvested cell sheets for various tissue reconstructions including ocular surfaces, periodontal ligament, cardiac patches as well as bladder.

Amniotic Stem Cell-Conditioned Media for the Treatment of Nerve and Muscle Pathology: A Systematic Review

2021 ◽  
Author(s):  
Chukwuweike Gwam ◽  
Ahmed Emara ◽  
Nequesha Mohamed ◽  
Noor Chughtai ◽  
Johannes Plate ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Tissue Regeneration ◽  
Cell Damage ◽  
Conditioned Media ◽  
Nerve Tissue ◽  
Muscle Pathology ◽  
Loss Of Function ◽  
Cell Based Therapy

Muscle and nerve tissue damage can elicit a significant loss of function and poses as a burden for patients and healthcare providers. Even for tissues, such as the peripheral nerve and skeletal muscle, that harbor significant regenerative capacity, innate regenerative processes often lead to less than optimal recovery and residual loss of function. The reasons for poor regeneration include significant cell damage secondary to oxidative stress, poor recruitment of resident stem cells, and an unfavorable microenvironment for tissue regeneration. Stem cell-based therapy was once thought as a potential therapy in tissue regeneration, due to its self-renewal and multipotent capabilities. Early advocates for cellular-based therapy pointed to the pluripotent nature of stem cells, thus eluding to its ability to differentiate into resident cells as the source of its regenerative capability. However, increasing evidence has revealed a lack of engraftment and differentiation of stem cells, thereby pointing to stem cell paracrine activity as being responsible for its regenerative potential. Stem cell-conditioned media houses biomolecular factors that portray significant regenerative potential. Amniotic-derived stem cell-conditioned media (AFS-CM) has been of particular interest because of its ease of allocation and in vitro culture. The purpose of this review is to report the results of studies that assess the role of AFS-CM for nerve and muscle conditions. In this review, we will cover the effects of AFS-CM on cellular pathways, genes, and protein expression for different nerve and muscle cell types.

scholarly journals Stem cell-derived cell sheet transplantation for heart tissue repair in myocardial infarction

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Rui Guo ◽  
Masatoshi Morimatsu ◽  
Tian Feng ◽  
Feng Lan ◽  
Dehua Chang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stem Cell ◽  
Tissue Repair ◽  
Cardiac Tissue ◽  
Cell Sheet ◽  
Clinical Manifestations ◽  
Induced Pluripotent Stem Cell ◽  
Heart Tissue ◽  
Therapeutic Effects ◽  
Cell Sheets

AbstractStem cell-derived sheet engineering has been developed as the next-generation treatment for myocardial infarction (MI) and offers attractive advantages in comparison with direct stem cell transplantation and scaffold tissue engineering. Furthermore, induced pluripotent stem cell-derived cell sheets have been indicated to possess higher potential for MI therapy than other stem cell-derived sheets because of their capacity to form vascularized networks for fabricating thickened human cardiac tissue and their long-term therapeutic effects after transplantation in MI. To date, stem cell sheet transplantation has exhibited a dramatic role in attenuating cardiac dysfunction and improving clinical manifestations of heart failure in MI. In this review, we retrospectively summarized the current applications and strategy of stem cell-derived cell sheet technology for heart tissue repair in MI.

Bulk poly(N-isopropylacrylamide) (PNIPAAm) thermoresponsive cell culture platform: toward a new horizon in cell sheet engineering

2019 ◽  
Vol 7 (6) ◽  
pp. 2277-2287 ◽  
Author(s):  
Andrew Choi ◽  
Kyoung Duck Seo ◽  
Hyungjun Yoon ◽  
Seon Jin Han ◽  
Dong Sung Kim
Keyword(s):  
Cell Culture ◽  
Cell Sheet ◽  
Cell Sheets ◽  
Cell Sheet Engineering

In contrast to the conventional ‘grafting’-based thermoresponsive cell culture platform, we first developed a bulk form of thermoresponsive cell culture platform for attaching/detaching diverse types and origins of the cell sheets in different shape.

The performance of bone marrow mesenchymal stem cell – Implant complexes prepared by cell sheet engineering techniques

Biomaterials ◽  
2010 ◽  
Vol 31 (12) ◽  
pp. 3212-3221 ◽  
Author(s):  
Wei Zhou ◽  
Chun Han ◽  
Yingliang Song ◽  
Xingrong Yan ◽  
Dehua Li ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Sheet ◽  
Cell Sheet Engineering

scholarly journals Designing 3D Mesenchymal Stem Cell Sheets Merging Magnetic and Fluorescent Features: When Cell Sheet Technology Meets Image-Guided Cell Therapy

Theranostics ◽  
2016 ◽  
Vol 6 (5) ◽  
pp. 739-751 ◽  
Author(s):  
Gabriel Rahmi ◽  
Laetitia Pidial ◽  
Amanda K. A. Silva ◽  
Eléonore Blondiaux ◽  
Bertrand Meresse ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Therapy ◽  
Cell Sheet ◽  
Cell Sheets ◽  
Image Guided ◽  
Cell Sheet Technology

scholarly journals Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering

2009 ◽  
Vol 6 (suppl_3) ◽  
Author(s):  
Kenichi Nagase ◽  
Jun Kobayashi ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Copolymer Composition ◽  
Cell Sheets ◽  
Chromatographic Separations ◽  
Cell Sheet Engineering ◽  
External Temperature ◽  
Cell Monolayers ◽  
Temperature Responsive ◽  
Confluent Cell ◽  
Dependent Cell

Temperature-responsive intelligent surfaces, prepared by the modification of an interface with poly( N -isopropylacrylamide) and its derivatives, have been used for biomedical applications. Such surfaces exhibit temperature-responsive hydrophilic/hydrophobic alterations with external temperature changes, which, in turn, result in thermally modulated interactions with biomolecules and cells. In this review, we focus on the application of these intelligent surfaces to chromatographic separation and cell cultures. Chromatographic separations using several types of intelligent surfaces are mentioned briefly, and various effects related to the separation of bioactive compounds are discussed, including wettability, copolymer composition and graft polymer architecture. Similarly, we also summarize temperature-responsive cell culture substrates that allow the recovery of confluent cell monolayers as contiguous living cell sheets for tissue-engineering applications. The key factors in temperature-dependent cell adhesion/detachment control are discussed from the viewpoint of grafting temperature-responsive polymers, and new methodologies for effective cell sheet culturing and the construction of thick tissues are summarized.

scholarly journals Stem cell application in rotator cuff repair: Interposition stem cell sheet versus overlaid stem cell sheet

2020 ◽  
Author(s):  
Jae hee Choi ◽  
Michael Seungcheol Kang ◽  
Myung Jin Shin ◽  
Dong Min Kim ◽  
Yu Na Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Rotator Cuff ◽  
Rotator Cuff Repair ◽  
Surgical Repair ◽  
Cell Sheet ◽  
Biomechanical Analysis ◽  
Control Group ◽  
Cell Sheets ◽  
Cuff Repair

Abstract Background Stem cells are an effective method of biologic healing and can be used to enhance the natural enthesis of the tendon-to-bone junction in rotator cuff repair. The purpose of this study was to investigate if the application of engineered stem cell sheets using adipose-derived cells (ADSCs) was effective in regeneration of natural enthesis and if there was a difference in the result of repair depending on the applied location Methods A chronic rotator cuff tear model was induced for 2 weeks, and cell sheets made using ADSCs isolated from rats were transplanted into the tendon-to-bone junction during surgical repair. Depending on the transplant location of the cell sheet, the difference in rotator cuff healing level between the overlaid group and the interposition group was compared to the surgical repair only group. The samples were obtained based on the tendon-to-bone junction and analysis of gross morphology, histology staining, and biomechanical analysis were performed. Results The differentiation potentials of ADSCs as stem cells were confirmed, as was the potential for tenogenic differentiation by growth factors. ADSCs were prepared as a sheet form to maintain the shape at the target site and to be easily attached. GFP-expressing ADSCs were proliferated in vivo and observed at the transplantation site. The overall healing level was better in the cell sheet transplanted group than in the control group that surgical repair only. Additionally, differences in healing level were shown depending on the cell sheet location by morphological, histological, and biomechanical perspectives. Histological results showed that the interposition transplantation group (1.75 ± 0.43, P = 0.004) showed better fibrocartilage formation and collagen orientation at the junction than the overlaid transplantation group (0.86 ± 0.83). Conclusion In the chronic rotator cuff repair model, the engineered stem cell sheets enhanced the regeneration of the tendon-to-bone junction. This regeneration was more effective when the stem cell sheet was interpositioned at the tendon-to-bone interface. Trial registration: Not applicable

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