scholarly journals Design and Biophysical Characterization of Poly (l-Lactic) Acid Microcarriers with and without Modification of Chitosan and Nanohydroxyapatite

Polymers ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1061 ◽  
Author(s):  
Liying Li ◽  
Kedong Song ◽  
Yongzhi Chen ◽  
Yiwei Wang ◽  
Fangxin Shi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Lactic Acid ◽  
Average Diameter ◽  
Natural Polymers ◽  
Promoting Effect ◽  
Biophysical Characterization ◽  
Promising Tool ◽  
Cell Carrier

Nowadays, microcarriers are widely utilized in drug delivery, defect filling, and cell culture. Also, many researchers focus on the combination of synthetic and natural polymers and bioactive ceramics to prepare composite biomaterials for tissue engineering and regeneration. In this study, three kinds of microcarriers were prepared based on physical doping and surface modification, named Poly (l-lactic) acid (PLLA), PLLA/nanohydroxyapatite (PLLA/nHA), and PLLA/nHA/Chitosan (PLLA/nHA/Ch). The physicochemical properties of the microcarriers and their functional performances in MC3T3-E1 cell culture were compared. Statistical results showed that the average diameter of PLLA microcarriers was 291.9 ± 30.7 μm, and that of PLLA/nHA and PLLA/nHA/Ch microcarriers decreased to 275.7 ± 30.6 μm and 269.4 ± 26.3 μm, respectively. The surface roughness and protein adsorption of microcarriers were enhanced with the doping of nHA and coating of chitosan. The cell-carrier cultivation stated that the PLLA/nHA microcarriers had the greatest proliferation-promoting effect, while the PLLA/nHA/Ch microcarriers performed the strongest attachment with MC3T3-E1 cells. Besides, the cells on the PLLA/nHA/Ch microcarriers exhibited optimal osteogenic expression. Generally, chitosan was found to improve microcarriers with superior characteristics in cell adhesion and differentiation, and nanohydroxyapatite was beneficial for microcarriers regarding sphericity and cell proliferation. Overall, the modified microcarriers may be considered as a promising tool for bone tissue engineering.

Investigation of a Collagen-Chitosan-Hydroxyapatite System for Novel Bone Substitutes

2007 ◽  
Vol 330-332 ◽  
pp. 415-418 ◽  
Author(s):  
Xiao Liang Wang ◽  
Xu Dong Li ◽  
Xiao Min Wang ◽  
Jian Lu ◽  
Hui Chuan Zhao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Substitutes ◽  
Nano Materials ◽  
Natural Polymers ◽  
Uniform Dispersion ◽  
Obvious Phase Separation ◽  
Ft Ir ◽  
Main Components

Collagen (Col) and chitosan (Chi) are both natural polymers and have received extensive investigation in recent years in the field of tissue engineering, but there are few reports on the introduction of hydroxyapatite (HA) into the Col-Ch system. In this study, based on the miscibility of these two polymers under proper condition, hydroxyapatite (HA) was synthesis in the Col-Chi system by in-situ co-precipitate method to give rise to a novel nanocomposite. The structural characterization of such Col-Ch-HA nano-materials was carried out by using FT-IR, XRD, SEM and TGA analyses with main components and Col-Chi samples used for comparison. It was found that there exist interactions between Col and Chi molecules. The nucleation and growth of inorganic phase occurs in the Col-Chi system and final products are uniform dispersion of nano-sized HA in the Col-Chi network without obvious phase separation. This novel nanocomposite would be a promising material for bone tissue engineering.

Preparation and characterization of alginate/HACC/oyster shell powder biocomposite scaffolds for potential bone tissue engineering applications

RSC Advances ◽  
2016 ◽  
Vol 6 (42) ◽  
pp. 35577-35588 ◽  
Author(s):  
Tai-ying Chen ◽  
Hao-chao Huang ◽  
Jia-lin Cao ◽  
Yan-jiao Xin ◽  
Wen-feng Luo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Repair ◽  
Oyster Shell ◽  
Natural Polymers ◽  
Tissue Engineering Scaffolds ◽  
Repair Materials ◽  
Oyster Shell Powder ◽  
Bone Repair Materials ◽  
Shell Powder

Tissue engineering scaffolds combining biominerals and natural polymers are prospective candidates for bone repair materials.

Characterization of maghemite (γ-Fe2O3)-loaded poly-l-lactic acid/thermoplastic polyurethane electrospun mats for soft tissue engineering

2016 ◽  
Vol 51 (18) ◽  
pp. 8361-8381 ◽  
Author(s):  
Ehsan Fallahiarezoudar ◽  
Mohaddeseh Ahmadipourroudposht ◽  
Ani Idris ◽  
Noordin Mohd Yusof ◽  
Mohsen Marvibaigi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Soft Tissue ◽  
Thermoplastic Polyurethane ◽  
Soft Tissue Engineering

scholarly journals In Vitro Characterization of Poly(Lactic Acid)/ Poly(Hydroxybutyrate)/ Thermoplastic Starch Blends for Tissue Engineering Application

2021 ◽  
Vol 30 ◽  
pp. 096368972110210
Author(s):  
Martina Culenova ◽  
Ivana Birova ◽  
Pavol Alexy ◽  
Paulina Galfyova ◽  
Andreas Nicodemou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Thermoplastic Starch ◽  
Biological Behavior ◽  
Poly Lactic Acid ◽  
Cytotoxicity Test ◽  
In Vitro Characterization ◽  
Custom Made

Complex in vitro characterization of a blended material based on Poly(Lactic Acid), Poly(Hydroxybutyrate), and Thermoplastic Starch (PLA/PHB/TPS) was performed in order to evaluate its potential for application in the field of tissue engineering. We focused on the biological behavior of the material as well as its mechanical and morphological properties. We also focused on the potential of the blend to be processed by the 3D printer which would allow the fabrication of the custom-made scaffold. Several blends recipes were prepared and characterized. This material was then studied in the context of scaffold fabrication. Scaffold porosity, wettability, and cell-scaffold interaction were evaluated as well. MTT test and the direct contact cytotoxicity test were applied in order to evaluate the toxic potential of the blended material. Biocompatibility studies were performed on the human chondrocytes. According to our results, we assume that material had no toxic effect on the cell culture and therefore could be considered as biocompatible. Moreover, PLA/PHB/TPS blend is applicable for 3D printing. Printed scaffolds had highly porous morphology and were able to absorb water as well. In addition, cells could adhere and proliferate on the scaffold surface. We conclude that this blend has potential for scaffold engineering.

Production and Characterization of Poly (Lactic Acid)/Nanostructured Carboapatite for 3D Printing of Bioactive Scaffolds for Bone Tissue Engineering

2021 ◽  
Author(s):  
Thiago Nunes Palhares ◽  
Lívia Rodrigues de Menezes ◽  
Gabriela Soares Kronemberger ◽  
Priscila Grion de Miranda Borchio ◽  
Leandra Santos Baptista ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Poly Lactic Acid ◽  
Bioactive Scaffolds

Fabrication and characterization of a collagen coated electrospun poly(3-hydroxybutyric acid)–gelatin nanofibrous scaffold as a soft bio-mimetic material for skin tissue engineering applications

RSC Advances ◽  
2016 ◽  
Vol 6 (10) ◽  
pp. 7914-7922 ◽  
Author(s):  
Giriprasath Ramanathan ◽  
Sivakumar Singaravelu ◽  
M. D. Raja ◽  
Naveen Nagiah ◽  
P. Padmapriya ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Engineering Application ◽  
Nanofibrous Scaffold ◽  
Skin Tissue ◽  
Tissue Engineering Application ◽  
Hydroxybutyric Acid ◽  
Skin Tissue Engineering ◽  
Promising Tool ◽  
Good Biocompatibility

The collagen coated nanofibrous scaffold mimics the function of the extra cellular matrix with good biocompatibility, cell adhesion, cell proliferation and aids to provide as a promising tool in skin tissue engineering application.

scholarly journals Characterization of biodegradable poly(lactic acid) porous scaffolds prepared using selective enzymatic degradation for tissue engineering

RSC Advances ◽  
2017 ◽  
Vol 7 (54) ◽  
pp. 34063-34070 ◽  
Author(s):  
Ziqi Guo ◽  
Cheng Yang ◽  
Zuping Zhou ◽  
Shan Chen ◽  
Fan Li
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Enzymatic Degradation ◽  
Poly Lactic Acid ◽  
Porous Scaffolds ◽  
Sem Images ◽  
Biodegradable Poly

SEM images of MEF cells on PLA scaffolds prepared by selective enzymatic degradation after 7 days of culture. The results demonstrated that MEF cells attached more easily to the surface than in the interior of the PLA scaffolds.

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