Fabrication and characterization of a collagen coated electrospun poly(3-hydroxybutyric acid)–gelatin nanofibrous scaffold as a soft bio-mimetic material for skin tissue engineering applications

RSC Advances ◽  
2016 ◽  
Vol 6 (10) ◽  
pp. 7914-7922 ◽  
Author(s):  
Giriprasath Ramanathan ◽  
Sivakumar Singaravelu ◽  
M. D. Raja ◽  
Naveen Nagiah ◽  
P. Padmapriya ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Engineering Application ◽  
Nanofibrous Scaffold ◽  
Skin Tissue ◽  
Tissue Engineering Application ◽  
Hydroxybutyric Acid ◽  
Skin Tissue Engineering ◽  
Promising Tool ◽  
Good Biocompatibility

The collagen coated nanofibrous scaffold mimics the function of the extra cellular matrix with good biocompatibility, cell adhesion, cell proliferation and aids to provide as a promising tool in skin tissue engineering application.

scholarly journals Preparation and Characterization of Sodium Alginate Polymeric Scaffold by Electrospinning Method for Skin Tissue Engineering Application

2021 ◽  
Author(s):  
Sorour Jadbabaei ◽  
Farid Naiemi ◽  
Hassan Ebadi-Dehaghani ◽  
Majid Kolahdoozan
Keyword(s):  
Tissue Engineering ◽  
Sodium Alginate ◽  
Engineering Application ◽  
L929 Cell ◽  
Skin Tissue ◽  
Tissue Engineering Application ◽  
Skin Tissue Engineering ◽  
Working Space ◽  
Polymeric Scaffold ◽  
High Viability

Abstract Sodium alginate (SA) approved its high potential in tissue engineering and regenerative medicine. One of the main weaknesses of this polysaccharide is its low spinnability which nanofiber based scaffolds are the interest of scientists in biomedical engineering. The main aim of this study was to improve the spinnability of SA in combination with polyvinyl alcohol (PVA). It was also tried to optimize the main parameters in electrospinning of the optimized SA;PVA ratio including voltage, flow rate, and working space. To aim this, Response surface methodology under central composite design was employed to design the experiments scientifically. The final nanofiber scaffolds were studied using scanning electron microscopy, Fourier transform infrared spectroscopy, degradability, swelling, tensile strength, porosity, nanofiber diameter, contact angle, and cytotoxicity. Based on the results, the best ratio for SA:PVA was 1:6.5. The solution with this concentration was spinnable in various values for the process parameters. The fabricated scaffolds under these conditions revealed good physical, chemical, mechanical, and biological features. L929 cell lines revealed high viability during 48 h of culture. The results revealed the uniform and homogeneous nanofibers with the regular size distribution (166 nm) were obtained at 30 kV, 0.55 µl/h, and 12.5 cm. To sum up, the optimized ratio under the reported conditions can be a good biologically compatible candidate for skin tissue engineering.

scholarly journals Skin Tissue Engineering: Application of Adipose-Derived Stem Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Agnes S. Klar ◽  
Jakub Zimoch ◽  
Thomas Biedermann
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Engineering Application ◽  
Stromal Vascular Fraction ◽  
Skin Tissue ◽  
Tissue Engineering Application ◽  
Adipose Derived Stem Cells ◽  
Skin Tissue Engineering ◽  
Stem Cell Source

Perception of the adipose tissue has changed dramatically over the last few decades. Identification of adipose-derived stem cells (ASCs) ultimately transformed paradigm of this tissue from a passive energy depot into a promising stem cell source with properties of self-renewal and multipotential differentiation. As compared to bone marrow-derived stem cells (BMSCs), ASCs are more easily accessible and their isolation yields higher amount of stem cells. Therefore, the ASCs are of high interest for stem cell-based therapies and skin tissue engineering. Currently, freshly isolated stromal vascular fraction (SVF), which may be used directly without any expansion, was also assessed to be highly effective in treating skin radiation injuries, burns, or nonhealing wounds such as diabetic ulcers. In this paper, we review the characteristics of SVF and ASCs and the efficacy of their treatment for skin injuries and disorders.

Hybrid PCL/chitosan-PEO nanofibrous scaffolds incorporated with A. euchroma extract for skin tissue engineering application

2021 ◽  
pp. 118926
Author(s):  
Fatemeh Asghari ◽  
Davood Rabiei Faradonbeh ◽  
Ziba Veisi Malekshahi ◽  
Houra Nekounam ◽  
Behnaz Ghaemi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Engineering Application ◽  
Skin Tissue ◽  
Tissue Engineering Application ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds
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