scholarly journals Decomposition of Calcium Oxalate Crystals in Colobanthus quitensis under CO2 Limiting Conditions

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1307
Author(s):  
Olman Gómez-Espinoza ◽  
Daniel González-Ramírez ◽  
Panagiota Bresta ◽  
George Karabourniotis ◽  
León A. Bravo
Keyword(s):  
Calcium Oxalate ◽  
Electron Transport Rate ◽  
Relative Size ◽  
Oxalate Oxidase ◽  
Stress Factors ◽  
Calcium Oxalate Crystals ◽  
Colobanthus Quitensis ◽  
Diffusion Limitations ◽  
Co2 Diffusion ◽  
The Antarctic

Calcium oxalate (CaOx) crystals are widespread among plant species. Their functions are not yet completely understood; however, they can provide tolerance against multiple environmental stress factors. Recent evidence suggested that CaOx crystals function as carbon reservoirs since its decomposition provides CO2 that may be used as carbon source for photosynthesis. This might be advantageous in plants with reduced mesophyll conductance, such as the Antarctic plant Colobanthus quitensis, which have shown CO2 diffusion limitations. In this study, we evaluate the effect of two CO2 concentrations in the CaOx crystals decomposition and chlorophyll fluorescence of C. quitensis. Plants were exposed to airflows with 400 ppm and 11.5 ppm CO2 and the number and relative size of crystals, electron transport rate (ETR), and oxalate oxidase (OxO) activity were monitored along time (10 h). Here we showed that leaf crystal area decreases over time in plants with 11.5 ppm CO2, which was accompanied by increased OxO activity and only a slight decrease in the ETR. These results suggested a relation between CO2 limiting conditions and the CaOx crystals decomposition in C. quitensis. Hence, crystal decomposition could be a complementary endogenous mechanism for CO2 supply in plants facing the Antarctic stressful habitat.

scholarly journals Calcium Oxalate Crystals in Leaves of the Extremophile Plant Colobanthus quitensis (Kunth) Bartl. (Caryophyllaceae)

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1787
Author(s):  
Olman Gómez-Espinoza ◽  
Daniel González-Ramírez ◽  
Jairo Méndez-Gómez ◽  
Rossy Guillén-Watson ◽  
Alejandro Medaglia-Mata ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Calcium Oxalate ◽  
Polarized Light ◽  
Common Garden ◽  
Ecotypic Differentiation ◽  
Colobanthus Quitensis ◽  
Precise Knowledge ◽  
Caox Crystal ◽  
Crystal Accumulation ◽  
The Antarctic

The presence of calcium oxalate (CaOx) crystals has been widely reported in the plant kingdom. These structures play a central role in various physiological functions, including calcium regulation, metal detoxification, and photosynthesis. However, precise knowledge about their possible roles and functions in plants is still limited. Therefore, the present work aims to study the ecotypic variability of Colobanthus quitensis, an extremophile species, concerning CaOx crystal accumulation. The CaOx crystals were studied in leaves of C. quitensis collected from different provenances within a latitudinal gradient (From Andes mountains in central Chile to Antarctica) and grown under common garden conditions. Polarized light microscopy, digital image analysis, and electron microscopy were used to characterize CaOx crystals. The presence of CaOx crystals was confirmed in the four provenances of C. quitensis, with significant differences in the accumulation among them. The Andean populations presented the highest accumulation of crystals and the Antarctic population the lowest. Electron microscopy showed that CaOx crystals in C. quitensis are classified as druses based on their morphology. The differences found could be linked to processes of ecotypic differentiation and plant adaptation to harsh environments.

Identification of calcium oxalate crystals in dried or fixed tobacco leaf

1984 ◽  
Vol 42 ◽  
pp. 288-289
Author(s):  
Vicki L. Baliga ◽  
Mary Ellen Counts
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Calcium Oxalate ◽  
Growth And Development ◽  
Polarized Light ◽  
Calcium Oxalate Crystals ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Electron

Calcium is an important element in the growth and development of plants and one form of calcium is calcium oxalate. Calcium oxalate has been found in leaf seed, stem material plant tissue culture, fungi and lichen using one or more of the following methods—polarized light microscopy (PLM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and x-ray diffraction.Two methods are presented here for qualitatively estimating calcium oxalate in dried or fixed tobacco (Nicotiana) leaf from different stalk positions using PLM. SEM, coupled with energy dispersive x-ray spectrometry (EDS), and powder x-ray diffraction were used to verify that the crystals observed in the dried leaf with PLM were calcium oxalate.

An SEM study of raphide crystal initials in the leaves of vitis (grape)

1995 ◽  
Vol 53 ◽  
pp. 984-985
Author(s):  
H. J. Arnott ◽  
M. A. Webb ◽  
L. E. Lopez
Keyword(s):  
Calcium Oxalate ◽  
Water Soluble ◽  
Calcium Oxalate Crystals ◽  
Calcium Oxalate Crystal ◽  
Oxalate Crystals ◽  
Large Numbers ◽  
Sem Study ◽  
The Matrix ◽  
Crystal Cells ◽  
Crystal Idioblast

Many papers have been published on the structure of calcium oxalate crystals in plants, however, few deal with the early development of crystals. Large numbers of idioblastic calcium oxalate crystal cells are found in the leaves of Vitis mustangensis, V. labrusca and V. vulpina. A crystal idioblast, or raphide cell, will produce 150-300 needle-like calcium oxalate crystals within a central vacuole. Each raphide crystal is autonomous, having been produced in a separate membrane-defined crystal chamber; the idioblast''s crystal complement is collectively embedded in a water soluble glycoprotein matrix which fills the vacuole. The crystals are twins, each having a pointed and a bidentate end (Fig 1); when mature they are about 0.5-1.2 μn in diameter and 30-70 μm in length. Crystal bundles, i.e., crystals and their matrix, can be isolated from leaves using 100% ETOH. If the bundles are treated with H2O the matrix surrounding the crystals rapidly disperses.

Oxalic acid and calcium oxalate produced by Leucostoma cincta and L. persoonii in culture and in peach bark tissues

1987 ◽  
Vol 65 (9) ◽  
pp. 1952-1956 ◽  
Author(s):  
J. A. Traquair
Keyword(s):  
Oxalic Acid ◽  
Calcium Oxalate ◽  
Crystal Morphology ◽  
Culture Media ◽  
Positive Staining ◽  
Acid Solutions ◽  
Calcium Oxalate Crystals ◽  
Oxalate Crystals ◽  
Electron Microscopes ◽  
Scanning Electron Microscopes

Oxalic acid and crystals of calcium oxalate were produced during growth of Leucostoma cincta and L. persoonii on potato dextrose agar and in peach bark tissues. The identification of calcium oxalate was based on solubility characteristics, the results of KMnO4 titration, positive staining with silver nitrate – dithiooxamide, and crystal morphology as observed with light and scanning electron microscopes. Oxalic acid was detected by gas chromatography. This is the first report of oxalic acid production by both Leucostoma species causing peach canker. Calcium oxalate crystals observed on or near hyphae in culture were similar to crystals in artificially inoculated peach bark tissues. Addition of oxalic acid solutions alone to inner bark tissues caused maceration and necrosis. These results indicate a role for oxalic acid in the early stages of pathogenesis by Leucostoma spp. Tetragonal (bipyramidal) and prismatic calcium oxalate crystals formed on bark wounds treated with oxalic acid solutions were similar to those observed in infected tissues and in culture media amended with oxalic acid.

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