scholarly journals Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete Phytophthora infestans, a Causative Agent of Potato Late Blight Disease

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1294
Author(s):  
Eugene A. Rogozhin ◽  
Alexey S. Vasilchenko ◽  
Anna S. Barashkova ◽  
Alexey N. Smirnov ◽  
Sergey K. Zavriev ◽  
...  
Keyword(s):  
Amino Acid ◽  
Medicinal Plants ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Causative Agent ◽  
Biologically Active ◽  
Peptide Fragments ◽  
Bisphosphate Carboxylase ◽  
Late Blight Disease ◽  
Blight Disease

We report the inhibitory effect of peptide extracts obtained from seven medicinal plants against a causative agent of late blight disease Phytophthora infestans. We find that all the extracts possess inhibitory activity toward the zoospores output, zoosporangium germination, and the development of P. infestans on potato disc tubers at different quantitative levels. Based on the biological effects detected, an extract of common horsetail (Equisetum arvense) biomass is recognized as the most effective and is selected for further structural analysis. We perform a combination of amino acid analysis and MALDI-TOF mass spectrometry, which reveal the presence of Asn/Asp- and Gln/Glu-rich short peptides with molecular masses in the range of 500–900 Da and not exceeding 1500 Da as the maximum. Analytical anion-exchange HPLC is successfully applied for separation of the peptide extract from common horsetail (E. arvense). We collect nine dominant components that are combined in two groups with differences in retention times. The N-terminal amino acid sequence of the prevalent compounds after analytical ion-exchange HPLC allows us to identify them as peptide fragments of functionally active proteins associated with photosynthesis, aquatic transport, and chitin binding. The anti-oomycete effects may be associated with the conversion of ribulose-1,5-bisphosphate carboxylase/oxygenase to produce a number of biologically active anionic peptides with possible regulatory functions. These data inform our knowledge regarding biologically active peptide fragments; they are the components of programmed or induced proteolysis of plant proteins and can realize secondary antimicrobial functions.

Evaluation of bio-agents and neem based products against late blight disease (Phytophthora infestans) of potato

2021 ◽  
Vol 74 (1) ◽  
pp. 181-187
Author(s):  
Mehi Lal ◽  
Sorabh Chaudhary ◽  
Sanjay Rawal ◽  
Sanjeev Sharma ◽  
Manoj Kumar ◽  
...  
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Late Blight Disease ◽  
Blight Disease

scholarly journals Phenotypic Characterization of Recent Clonal Lineages of Phytophthora infestans in the United States

Plant Disease ◽  
2013 ◽  
Vol 97 (7) ◽  
pp. 873-881 ◽  
Author(s):  
G. Danies ◽  
I. M. Small ◽  
K. Myers ◽  
R. Childers ◽  
W. E. Fry
Keyword(s):  
United States ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
The United States ◽  
Phenotypic Characterization ◽  
Phenotypic Traits ◽  
Mating Types ◽  
Late Blight Disease ◽  
Blight Disease

Phytophthora infestans, the causal agent of late blight disease, has been reported in the United States and Canada since the mid-nineteenth century. Due to the lack of or very limited sexual reproduction, the populations of P. infestans in the United States are primarily reproducing asexually and, thus, show a simple genetic structure. The emergence of new clonal lineages of P. infestans (US-22, US-23, and US-24) responsible for the late blight epidemics in the northeastern region of the United States in the summers of 2009 and 2010 stimulated an investigation into phenotypic traits associated with these genotypes. Mating type, differences in sensitivity to mefenoxam, differences in pathogenicity on potato and tomato, and differences in rate of germination were studied for clonal lineages US-8, US-22, US-23, and US-24. Both A1 and A2 mating types were detected. Lineages US-22, US-23, and US-24 were generally sensitive to mefenoxam while US-8 was resistant. US-8 and US-24 were primarily pathogenic on potato while US-22 and US-23 were pathogenic on both potato and tomato. Indirect germination was favored at lower temperatures (5 and 10°C) whereas direct germination, though uncommon, was favored at higher temperatures (20 and 25°C). Sporangia of US-24 released zoospores more rapidly than did sporangia of US-22 and US-23. The association of characteristic phenotypic traits with genotype enables the prediction of phenotypic traits from rapid genotypic analyses for improved disease management.

Field application of Bacillus subtilis isolates for controlling late blight disease of potato caused by Phytophthora infestans

2019 ◽  
Vol 22 ◽  
pp. 101366 ◽  
Author(s):  
Bhimanagoud Kumbar ◽  
Riaz Mahmood ◽  
S.N. Nagesha ◽  
M.S. Nagaraja ◽  
D.G. Prashant ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Field Application ◽  
Late Blight Disease ◽  
Blight Disease

Identification and Screening of Introduced Tomato Varieties Resistant to late Blight

2012 ◽  
Vol 610-613 ◽  
pp. 3472-3477
Author(s):  
Dan Luo ◽  
Xi Chun Zhang ◽  
Xiao Han Wen
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
New Disease ◽  
Tomato Production ◽  
Disease Resistant ◽  
Resistant Varieties ◽  
Detached Leaves ◽  
Late Blight Disease ◽  
Blight Disease ◽  
Selection Of

Tomato late blight disease that infected by Phytophthora infestans (Mont.) de Bary has already become one of the main obstacles that influence the tomato production. To resolve tomato late blight disease, cultivating the disease-resistant breed is importance. Based on the previous study, different introduced varieties were used as materials in this test for the identification and varieties selection of tomato resistant to late blight, by measuring the DIS of inoculated seedlings and detached leaves. It indicated that Jingle 502 and O-33-1 are possible to become resistant varieties to late blight or the parents for cultivating new disease resistant varieties.

scholarly journals Genotype reaction of Solanum tuberosum, andigena and phureja groups to late blight (Phytophthora infestans Mont. De Bary)

2019 ◽  
Vol 36 (E) ◽  
pp. 138-157
Author(s):  
Jennyfer Moncayo ◽  
Roberth Delgado ◽  
Carlos Marcillo ◽  
Claudia Salazar ◽  
Carlos Betancourth
Keyword(s):  
Solanum Tuberosum ◽  
Late Blight ◽  
Phytophthora Infestans ◽  
Potato Crop ◽  
Block Design ◽  
Food Product ◽  
Randomized Complete Block Design ◽  
Late Blight Disease ◽  
Disease Effect ◽  
Blight Disease

Potato crop is the fourth main food product in the world, but is affected by Phytophthora infestans, the late blight disease causal agent. This research was carried out in a town of Pasto high plateau, South of Colombia. In order to evaluate the reaction of different genotypes of creole potato (Solanum tuberosum groups Phureja and Andigena) with regard the disease. A Randomized Complete Block design was established with three repetitions, where 30 genotypes were distributed and three plants were evaluated per repetition during two crop cycles in natural exposure to the pathogen. To assess the disease effect on production, the same genotypes were maintained with chemical control in an adjacent batch. Genotypes classified as tolerant are Chaucha Manzana, Cachuda, Criolla Colombia, Malvaseña, Andina and Criolla, during the first cycle with performance values ​​between 23 to 26t.ha-1 and during the second to Calavera Negra, Chaucha Paisa, Ratona, Criolla Galeras, Ratona Roja, Tornilla Roja and Aguacata with performance values ​​between 23 to 28t.ha-1. The graphic severity scale allowed genotypes to be categorized as susceptible and highly susceptible. The results indicate that for the prevalent pathogen race in this study area, there are no resistance sources within the studied collection. However, genotypes such as Criolla Colombia and Andina showed a better performance in terms of yield.

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