scholarly journals Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 941
Author(s):  
Xuejun Lin ◽  
Ruimei Li ◽  
Yangjiao Zhou ◽  
Fenlian Tang ◽  
Yajie Wang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Stress Responses ◽  
Transgenic Arabidopsis ◽  
Salt Bridge ◽  
Auxin Signaling ◽  
Storage Roots ◽  
Calcium Dependent ◽  
Auxin Signaling Pathway ◽  
Mda Content ◽  
Regulatory Effects

Annexins are a superfamily of soluble calcium-dependent phospholipid-binding proteins that have considerable regulatory effects in plants, especially in response to adversity and stress. The Arabidopsis thaliana AtAnn1 gene has been reported to play a significant role in various abiotic stress responses. In our study, the cDNA of an annexin gene highly similar to AtAnn1 was isolated from the cassava genome and named MeAnn2. It contains domains specific to annexins, including four annexin repeat sequences (I–IV), a Ca2+-binding sequence, Ca2+-independent membrane-binding-related tryptophan residues, and a salt bridge-related domain. MeAnn2 is localized in the cell membrane and cytoplasm, and it was found to be preferentially expressed in the storage roots of cassava. The overexpression of MeAnn2 reduced the sensitivity of transgenic Arabidopsis to various Ca2+, NaCl, and indole-3-acetic acid (IAA) concentrations. The expression of the stress resistance-related gene AtRD29B and auxin signaling pathway-related genes AtIAA4 and AtLBD18 in transgenic Arabidopsis was significantly increased under salt stress, while the Malondialdehyde (MDA) content was significantly lower than that of the control. These results indicate that the MeAnn2 gene may increase the salt tolerance of transgenic Arabidopsis via the IAA signaling pathway.

scholarly journals Overexpression of UGT74E2, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice

2020 ◽  
Vol 21 (19) ◽  
pp. 7239
Author(s):  
Ting Wang ◽  
Pan Li ◽  
Tianjiao Mu ◽  
Guangrui Dong ◽  
Chengchao Zheng ◽  
...  
Keyword(s):  
Seed Germination ◽  
Stress Tolerance ◽  
Signaling Pathway ◽  
Stress Responses ◽  
Auxin Signaling ◽  
Rice Seed ◽  
Aba Signaling ◽  
Auxin Signaling Pathway ◽  
Pathway Gene ◽  
Drought And Salt Stresses

UDP-glycosyltransferases (UGTs) play key roles in modulating plant development and responses to environmental challenges. Previous research reported that the Arabidopsis UDP-glucosyltransferase 74E2 (AtUGT74E2), which transfers glucose to indole-3-butyric acid (IBA), is involved in regulating plant architecture and stress responses. Here, we show novel and distinct roles of UGT74E2 in rice. We found that overexpression of AtUGT74E2 in rice could enhance seed germination. This effect was also observed in the presence of IBA and abscisic acid (ABA), as well as salt and drought stresses. Further investigation indicated that the overexpression lines had lower levels of free IBA and ABA compared to wild-type plants. Auxin signaling pathway gene expression such as for OsARF and OsGH3 genes, as well as ABA signaling pathway genes OsABI3 and OsABI5, was substantially downregulated in germinating seeds of UGT74E2 overexpression lines. Consistently, due to reduced IBA and ABA levels, the established seedlings were less tolerant to drought and salt stresses. The regulation of rice seed germination and stress tolerance could be attributed to IBA and ABA level alterations, as well as modulation of the auxin/ABA signaling pathways by UGT74E2. The distinct roles of UGT74E2 in rice implied that complex and different molecular regulation networks exist between Arabidopsis and rice.

scholarly journals A chemically induced proximity system engineered from the plant auxin signaling pathway

2018 ◽  
Vol 9 (26) ◽  
pp. 5822-5827 ◽  
Author(s):  
Weiye Zhao ◽  
Huong Nguyen ◽  
Guihua Zeng ◽  
Dan Gao ◽  
Hao Yan ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Auxin Signaling ◽  
Auxin Signaling Pathway ◽  
Chemically Induced

A new chemically induced proximity system is developed by engineering the plant auxin signaling pathway.

Identification of Auxin Activity Like 1, a chemical with weak functions in auxin signaling pathway

2018 ◽  
Vol 98 (3) ◽  
pp. 275-287
Author(s):  
Wenbo Li ◽  
Haimin Li ◽  
Peng Xu ◽  
Zhi Xie ◽  
Yajin Ye ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Auxin Signaling ◽  
Auxin Activity ◽  
Auxin Signaling Pathway

scholarly journals Molecular Rewiring of the Jasmonate Signaling Pathway to Control Auxin-Responsive Gene Expression

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 641
Author(s):  
Ning Li ◽  
Linggai Cao ◽  
Wenzhuo Miu ◽  
Ruibin Cao ◽  
Mingbo Peng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signaling Pathway ◽  
Developmental Process ◽  
Expression System ◽  
Transient Gene Expression ◽  
Transcriptional Repressor ◽  
Auxin Signaling ◽  
General Strategy ◽  
Auxin Signaling Pathway ◽  
Responsive Gene

The plant hormone jasmonic acid (JA) has an important role in many aspects of plant defense response and developmental process. JA triggers interaction between the F-box protein COI1 and the transcriptional repressors of the JAZ family that leads the later to proteasomal degradation. The Jas-motif of JAZs is critical for mediating the COI1 and JAZs interaction in the presence of JA. Here, by using the protoplast transient gene expression system we reported that the Jas-motif of JAZ1 was necessary and sufficient to target a foreign reporter protein for COI1-facilitated degradation. We fused the Jas-motif to the SHY2 transcriptional repressor of auxin signaling pathway to create a chimeric protein JaSHY. Interestingly, JaSHY retained the transcriptional repressor function while become degradable by the JA coreceptor COI1 in a JA-dependent fashion. Moreover, the JA-induced and COI1-facilitated degradation of JaSHY led to activation of a synthetic auxin-responsive promoter activity. These results showed that the modular components of JA signal transduction pathway can be artificially redirected to regulate auxin signaling pathway and control auxin-responsive gene expression. Our work provides a general strategy for using synthetic biology approaches to explore and design cell signaling networks to generate new cellular functions in plant systems.

scholarly journals A Novel AP2-Type Transcription Factor, SMALL ORGAN SIZE1, Controls Organ Size Downstream of an Auxin Signaling Pathway

2014 ◽  
Vol 55 (5) ◽  
pp. 897-912 ◽  
Author(s):  
Koichiro Aya ◽  
Tokunori Hobo ◽  
Kanna Sato-Izawa ◽  
Miyako Ueguchi-Tanaka ◽  
Hidemi Kitano ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Signaling Pathway ◽  
Organ Size ◽  
Auxin Signaling ◽  
Auxin Signaling Pathway

scholarly journals A ROP GTPase-Dependent Auxin Signaling Pathway Regulates the Subcellular Distribution of PIN2 in Arabidopsis Roots

2012 ◽  
Vol 22 (14) ◽  
pp. 1319-1325 ◽  
Author(s):  
Deshu Lin ◽  
Shingo Nagawa ◽  
Jisheng Chen ◽  
Lingyan Cao ◽  
Xu Chen ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Subcellular Distribution ◽  
Auxin Signaling ◽  
Auxin Signaling Pathway ◽  
Rop Gtpase

scholarly journals Transcriptome Analysis Reveals Multiple Hormones, Wounding and Sugar Signaling Pathways Mediate Adventitious Root Formation in Apple Rootstock

2018 ◽  
Vol 19 (8) ◽  
pp. 2201 ◽  
Author(s):  
Ke Li ◽  
Yongqi Liang ◽  
Libo Xing ◽  
Jiangping Mao ◽  
Zhen Liu ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Signalling Pathways ◽  
Auxin Signaling ◽  
Strong Positive Correlation ◽  
Sugar Signaling ◽  
Apple Rootstocks ◽  
Auxin Signaling Pathway

Adventitious roots (AR) play an important role in the vegetative propagation of apple rootstocks. The potential role of hormone, wounding, and sugar signalling pathways in mediating AR formation has not been adequately explored and the whole co-expression network in AR formation has not been well established in apple. In order to identify the molecular mechanisms underlying AR formation in ‘T337’ apple rootstocks, transcriptomic changes that occur during four stages of AR formation (0, 3, 9 and 16 days) were analyzed using high-throughput sequencing. A total of 4294 differentially expressed genes were identified. Approximately 446 genes related to hormones, wounding, sugar signaling, root development, and cell cycle induction pathways were subsequently selected based on their potential to be involved in AR formation. RT-qPCR validation of 47 genes with known functions exhibited a strong positive correlation with the RNA-seq data. Interestingly, most of the candidate genes involved in AR formation that were identified by transcriptomic sequencing showed auxin-responsive expression patterns in an exogenous Indole-3-butyric acid (IBA)-treatment assay: Indicating that endogenous and exogenous auxin plays key roles in regulating AR formation via similar signalling pathways to some extent. In general, AR formation in apple rootstocks is a complex biological process which is mainly influenced by the auxin signaling pathway. In addition, multiple hormones-, wounding- and sugar-signaling pathways interact with the auxin signaling pathway and mediate AR formation in apple rootstocks.

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