scholarly journals A chemically induced proximity system engineered from the plant auxin signaling pathway

2018 ◽  
Vol 9 (26) ◽  
pp. 5822-5827 ◽  
Author(s):  
Weiye Zhao ◽  
Huong Nguyen ◽  
Guihua Zeng ◽  
Dan Gao ◽  
Hao Yan ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Auxin Signaling ◽  
Auxin Signaling Pathway ◽  
Chemically Induced

A new chemically induced proximity system is developed by engineering the plant auxin signaling pathway.

Identification of Auxin Activity Like 1, a chemical with weak functions in auxin signaling pathway

2018 ◽  
Vol 98 (3) ◽  
pp. 275-287
Author(s):  
Wenbo Li ◽  
Haimin Li ◽  
Peng Xu ◽  
Zhi Xie ◽  
Yajin Ye ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Auxin Signaling ◽  
Auxin Activity ◽  
Auxin Signaling Pathway

scholarly journals Molecular Rewiring of the Jasmonate Signaling Pathway to Control Auxin-Responsive Gene Expression

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 641
Author(s):  
Ning Li ◽  
Linggai Cao ◽  
Wenzhuo Miu ◽  
Ruibin Cao ◽  
Mingbo Peng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Signaling Pathway ◽  
Developmental Process ◽  
Expression System ◽  
Transient Gene Expression ◽  
Transcriptional Repressor ◽  
Auxin Signaling ◽  
General Strategy ◽  
Auxin Signaling Pathway ◽  
Responsive Gene

The plant hormone jasmonic acid (JA) has an important role in many aspects of plant defense response and developmental process. JA triggers interaction between the F-box protein COI1 and the transcriptional repressors of the JAZ family that leads the later to proteasomal degradation. The Jas-motif of JAZs is critical for mediating the COI1 and JAZs interaction in the presence of JA. Here, by using the protoplast transient gene expression system we reported that the Jas-motif of JAZ1 was necessary and sufficient to target a foreign reporter protein for COI1-facilitated degradation. We fused the Jas-motif to the SHY2 transcriptional repressor of auxin signaling pathway to create a chimeric protein JaSHY. Interestingly, JaSHY retained the transcriptional repressor function while become degradable by the JA coreceptor COI1 in a JA-dependent fashion. Moreover, the JA-induced and COI1-facilitated degradation of JaSHY led to activation of a synthetic auxin-responsive promoter activity. These results showed that the modular components of JA signal transduction pathway can be artificially redirected to regulate auxin signaling pathway and control auxin-responsive gene expression. Our work provides a general strategy for using synthetic biology approaches to explore and design cell signaling networks to generate new cellular functions in plant systems.

scholarly journals A Novel AP2-Type Transcription Factor, SMALL ORGAN SIZE1, Controls Organ Size Downstream of an Auxin Signaling Pathway

2014 ◽  
Vol 55 (5) ◽  
pp. 897-912 ◽  
Author(s):  
Koichiro Aya ◽  
Tokunori Hobo ◽  
Kanna Sato-Izawa ◽  
Miyako Ueguchi-Tanaka ◽  
Hidemi Kitano ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Signaling Pathway ◽  
Organ Size ◽  
Auxin Signaling ◽  
Auxin Signaling Pathway

scholarly journals A ROP GTPase-Dependent Auxin Signaling Pathway Regulates the Subcellular Distribution of PIN2 in Arabidopsis Roots

2012 ◽  
Vol 22 (14) ◽  
pp. 1319-1325 ◽  
Author(s):  
Deshu Lin ◽  
Shingo Nagawa ◽  
Jisheng Chen ◽  
Lingyan Cao ◽  
Xu Chen ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Subcellular Distribution ◽  
Auxin Signaling ◽  
Auxin Signaling Pathway ◽  
Rop Gtpase

scholarly journals Transcriptome Analysis Reveals Multiple Hormones, Wounding and Sugar Signaling Pathways Mediate Adventitious Root Formation in Apple Rootstock

2018 ◽  
Vol 19 (8) ◽  
pp. 2201 ◽  
Author(s):  
Ke Li ◽  
Yongqi Liang ◽  
Libo Xing ◽  
Jiangping Mao ◽  
Zhen Liu ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Signalling Pathways ◽  
Auxin Signaling ◽  
Strong Positive Correlation ◽  
Sugar Signaling ◽  
Apple Rootstocks ◽  
Auxin Signaling Pathway

Adventitious roots (AR) play an important role in the vegetative propagation of apple rootstocks. The potential role of hormone, wounding, and sugar signalling pathways in mediating AR formation has not been adequately explored and the whole co-expression network in AR formation has not been well established in apple. In order to identify the molecular mechanisms underlying AR formation in ‘T337’ apple rootstocks, transcriptomic changes that occur during four stages of AR formation (0, 3, 9 and 16 days) were analyzed using high-throughput sequencing. A total of 4294 differentially expressed genes were identified. Approximately 446 genes related to hormones, wounding, sugar signaling, root development, and cell cycle induction pathways were subsequently selected based on their potential to be involved in AR formation. RT-qPCR validation of 47 genes with known functions exhibited a strong positive correlation with the RNA-seq data. Interestingly, most of the candidate genes involved in AR formation that were identified by transcriptomic sequencing showed auxin-responsive expression patterns in an exogenous Indole-3-butyric acid (IBA)-treatment assay: Indicating that endogenous and exogenous auxin plays key roles in regulating AR formation via similar signalling pathways to some extent. In general, AR formation in apple rootstocks is a complex biological process which is mainly influenced by the auxin signaling pathway. In addition, multiple hormones-, wounding- and sugar-signaling pathways interact with the auxin signaling pathway and mediate AR formation in apple rootstocks.

scholarly journals Knockdown of miR393 Promotes the Growth and Biomass Production in Poplar

2021 ◽  
Vol 12 ◽  
Author(s):  
Liwei Chu ◽  
Xuejiao He ◽  
Wenbo Shu ◽  
Lijuan Wang ◽  
Fang Tang
Keyword(s):  
Signaling Pathway ◽  
Biomass Production ◽  
Lignin Content ◽  
Plant Root ◽  
Secondary Growth ◽  
Auxin Signaling ◽  
Optimal Size ◽  
Auxin Signaling Pathway ◽  
Cell Layers ◽  
Chemical Determination

Short tandem target mimic (STTM), which is composed of two short sequences mimicking small RNA target sites, separated by a linker of optimal size, can block the functions of all members in a miRNA family. microRNA393 (miR393), which is one of the conserved miRNA families in plants, can regulate plant root growth, leaf development, plant architecture, and stress resistance. In order to verify the role of miR393 in the secondary growth of trees, we created its STTM transgenic poplar lines (STTM393). The expression of miR393 in STTM393 lines was reduced by over 10 times compared with the control plants. STTM393 lines showed promoted growth with about 20% higher, 15% thicker, and 2–4 more internodes than the control plants after 3 months of growth. The cross-section of the stems showed that STTM393 lines had wider phloem, xylem, and more cambium cell layers than control plants, and the lignin content in STTM393 lines was also higher as revealed by staining and chemical determination. Based on the transcriptome analysis, the genes related to the auxin signaling pathway, cell cyclin, cell expansion, and lignin synthesis had higher expression in STTM393 lines than that in control plants. The higher expression levels of FBL family members suggested that the auxin signaling pathway was strengthened in STTM393 lines to promote plant growth. Therefore, the knockdown of miR393 using the STTM approach provides a way to improve poplar growth and biomass production.

scholarly journals CsMYB4a from Camellia sinensis Regulates the Auxin Signaling Pathway by Interacting with CsIAA4

2021 ◽  
Author(s):  
Guoliang Ma ◽  
Yingling Wu ◽  
Changjuan Jiang ◽  
Yifan Chen ◽  
Dawei Xing ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Signaling Pathway ◽  
Transgenic Tobacco ◽  
Camellia Sinensis ◽  
Auxin Signaling ◽  
Homologous Gene ◽  
Naphthaleneacetic Acid ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Auxin Signaling Pathway

Members of the R2R3-MYB4 subgroup are well-known negative regulatory transcription factors of phenylpropane and lignin pathways. In this study, we found that transgenic tobacco plants overexpressing a R2R3-MYB4 subgroup gene from Camellia sinensis (CsMYB4a) showed inhibited growth that was not regulated by phenylpropane and lignin pathways, and these plants exhibited altered sensitivity to synthetic auxin 1-naphthaleneacetic acid (α-NAA) treatment. An auxin/indole-3-acetic acid 4 (AUX/IAA4) gene from Camellia sinensis (CsIAA4) participating in the regulation of the auxin signal transduction pathway was screened from the yeast two-hybrid library with CsMYB4a as the bait protein, and tobacco plants overexpressing this gene showed a series of auxin-deficiency phenotypes, such as dwarfism, small leaves, reduced lateral roots, and a shorter primary root. CsIAA4 transgenic tobacco plants were less sensitive to exogenous α-NAA than control plants, which was consistent with the findings for CsMYB4a transgenic tobacco plants. The knockout of the endogenous NtIAA4 gene (a CsIAA4 homologous gene) in tobacco plants alleviated growth inhibition in CsMYB4a transgenic tobacco plants. Furthermore, protein-protein interaction experiments proved that domain II of CsIAA4 is the key motif for the interaction between CsIAA4 and CsMYB4a and that the degradation of CsIAA4 is prevented when CsMYB4a interacts with CsIAA4. In summary, our results suggest that CsMYB4a is a multifunctional transcription factor that regulates the auxin signaling pathway, phenylpropane and lignin pathways. This study provides new insights into the multiple functions of R2R3-MYB4 subgroup members as a group of well-known negative regulatory transcription factors.

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