scholarly journals Date Palm Extract (Phoenix dactylifera) PEGylated Nanoemulsion: Development, Optimization and Cytotoxicity Evaluation

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 735
Author(s):  
Hany Ezzat Khalil ◽  
Nashi K. Alqahtani ◽  
Hossam M. Darrag ◽  
Hairul-Islam Mohamed Ibrahim ◽  
Promise M. Emeka ◽  
...  
Keyword(s):  
Particle Size ◽  
Serum Protein ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Qualitative Assessment ◽  
Total Serum Protein ◽  
Total Serum ◽  
Total Phenolic ◽  
Palm Fruit

Date palm fruit (Phoenix dactylifera) is reputed to have numerous biological activities, including anticancer properties. To utilize the great fortune of this fruit, the current study aimed to maximize its pharmacological activity. Date palm extract (DPE) of Khalas cultivar was obtained in powder form and then was formulated into nanoemulsion (NE). The optimized DPE-NE was formulated along with its naked counterpart followed by studying their physical and chemical properties. A qualitative assessment of total serum protein associated with the surface of formulations was implemented. Studies for the in vitro release of DPE from developed NE before and after incubation with serum were investigated. Eventually, an MTT assay was conducted. Total phenolic and flavonoid contents were 22.89 ± 0.013 mg GAE/g of dry DPE and 9.90 ± 0.03 mg QE/g of dry DPE, respectively. Homogenous NE formulations were attained with appropriate particle size and viscosity that could be administered intravenously. The optimized PEGylated NE exhibited a proper particle size, PDI, and zeta potential. Total serum protein adsorbed on PEG-NE surface was significantly low. The release of the drug through in vitro study was effectively extended for 24 h. Ultimately; PEGylated NE of DPE attained significant inhibition for cancer cell viability with IC50 values of 18.6 ± 2.4 and 13.5 ± 1.8 µg/mL for MCF-7 and HepG2 cell lines, respectively. PEGylated NE of DPE of Khalas cultivar will open the gate for future adjuvants for cancer therapy.

scholarly journals Antioxidant Activity of Date Palm Fruit (Phoenix dactylifera L.) Extract for Oxidative Stabilisation of Butter Oil at Ambient Temperature

2015 ◽  
Vol 58 (2) ◽  
pp. 59-64
Author(s):  
Fazal Rahman ◽  
Muhammad Nadeem ◽  
Sabir Khan ◽  
Shakeel Ahmad ◽  
Yasir Zahoor
Keyword(s):  
Linoleic Acid ◽  
Ambient Temperature ◽  
Date Palm ◽  
Radical Scavenging ◽  
Phoenix Dactylifera ◽  
Oxidation Products ◽  
Total Phenolic ◽  
Palm Fruit ◽  
Butter Oil ◽  
Phoenix Dactylifera L

In this study, long term preservation of butter oil was achieved through ethanolic extract of date palm fruit (Phoenix dactylifera L.). Butter oil was supplemented with date palm fruit extract (DPFE) at three different concentrations i.e. 250, 500 and 750 ppm (T1, T2 and T3) and compared with a control. Total phenolic content, DPPH free radical scavenging activity and inhibition of linoleic acid peroxidation of the DPFE was 5.19 GAE, 74.2 and 81%, respectively. IC50 value of date extract for the inhibition of DPPH and linoleic acid peroxidation was 2.45 and 0.82 mg/mL, respectively. The loss of oleic acid and linoleic acid in control after six months of storage was 16 and 52% as compared to T3 which was 4% and 14%. T3 yielded the lowest concentration of primary and secondary oxidation products with no effect on sensory attributes. DPFE can be used to enhance the shelf life of  butter oil at ambient temperature. 

scholarly journals An extract from date palm fruit (Phoenix dactylifera) acts as a co-agonist ligand for the nuclear receptor FXR and differentially modulates FXR target-gene expression in vitro

PLoS ONE ◽  
2018 ◽  
Vol 13 (1) ◽  
pp. e0190210 ◽  
Author(s):  
Emilia Alfaro-Viquez ◽  
Brent F. Roling ◽  
Christian G. Krueger ◽  
Charlene J. Rainey ◽  
Jess D. Reed ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nuclear Receptor ◽  
Target Gene ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Palm Fruit ◽  
Target Gene Expression

Standardization of serum fructosamine assays

1990 ◽  
Vol 36 (1) ◽  
pp. 136-139 ◽  
Author(s):  
E D Schleicher ◽  
B W Vogt
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Hplc Method ◽  
Total Serum Protein ◽  
Total Serum ◽  
Binding Characteristics ◽  
Elementary Analysis ◽  
Glycated Proteins ◽  
Primary Standards

Abstract We have calibrated a secondary serum protein standard by use (as primary standards) of samples of albumin and polylysine glycated with [14C]glucose in vitro, the glycation of which was assessed by radioactivity measurements and by elementary analysis for C and N. Using this standard for calibration in our improved fructosamine assay, one obtains an average fructosamine value of 247 mumol/L for nondiabetic individuals (or, in terms of total serum protein, 3.2 mumol/g)--about a tenth the value we obtained when we used the fructosamine assay of Johnson et al. (Clin Chim Acta 1983;127:87-95), standardized with desoxymorpholinofructose. In contrast, results corresponded well with the value for mean glycation of serum proteins, 3 mumol/g, determined by a furosine/HPLC method. Evidently the proposed procedure, in which a standard sharing the binding characteristics of endogenous glycated proteins is used together with our modified new fructosamine assay, leads to more realistic values for the concentrations of glycated serum proteins.

Anthelmintic Effect of Date Palm Fruit: A Systematic Review

2018 ◽  
Vol 17 (3) ◽  
pp. 276-281
Author(s):  
A. Rauf ◽  
F.H. Addnan ◽  
N.F.M. Manzor ◽  
W.O. Abdullah ◽  
M.A. Elkadi ◽  
...  
Keyword(s):  
Systematic Review ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Economic Losses ◽  
Trichuris Muris ◽  
Palm Fruit ◽  
Parasitic Helminths ◽  
Anthelmintic Effect

Soil-transmitted helminth infections constitute a global affliction affecting people and livestock, resulting in a significant disease burden and economic losses in endemic regions. However, control has not been entirely successful and the problem is compounded by the development of resistance to available chemotherapy. Novel alternative compounds are urgently needed, encouraging the screening of largely plant-based sources for anthelmintic effects. Date palm fruit (Phoenix dactylifera) is a popular and nutritious food much coveted for its nutraceutical effect. There are convincing reports documenting the antibacterial, antiviral and antifungal effects of date palm fruit but evidence for any potential anthelmintic effect remains unclear. Here we conducted a systematic review of the published information through an electronic search using six scientific databases; SCOPUS, MEDLINE COMPLETE, OVID, EMBASE, WEB OF SCIENCE and CINAHL COMPLETE. Out of 58 studies that were initially identified only 2 manuscripts fitted our inclusion criteria. The first article reported on the anthelmintic effect of date palm fruit against the rodent nematodes Trichuris muris and Angiostrongylus cantonensis in vitro and in vivo while the second article was based only on in vivo assessment (with coconut) of the effect on the rodent cestode Hymenolepis microstoma. Our review revealed limited anecdotal reports of the anthelmintic effect of date palm fruit against parasitic helminths, however, the majority of reports were inconclusive due to mixed results, limited focus on date palm fruit and lack of any statistical analyses. Rigorous, systematic and fully controlled studies focusing on date palm fruit are needed to validate the anecdotal evidence.

PARALLEL STUDIES OF COMPLEMENT AND COAGULATION IV. EFFECT OF CARBON TETRACHLORIDE

1951 ◽  
Vol 29 (2) ◽  
pp. 48-58
Author(s):  
Christine E. Rice ◽  
Paul Boulanger ◽  
P. J. G. Plummer
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Serum Protein ◽  
Guinea Pigs ◽  
Fatty Degeneration ◽  
Blood Groups ◽  
Total Serum Protein ◽  
Total Serum ◽  
Coagulation Time ◽  
Complement Activity

To determine whether liver injury would result in a parallel decline in the complement titer and coagulative properties of the blood, groups of guinea pigs were given series of injections of the liver poison, carbon tetrachloride. Marked fatty degeneration of the liver, a decline in total serum protein and albumin, a decrease in complement activity, and a prolongation of coagulation time was observed in the treated animals. A general relationship was noted between the albumin-globulin ratio and the complement titer of the serum and between the complement titer and the coagulation time of the plasma.

Muscle Weight Relationship to Serum Proteins, Hemocytes, and Hepatopancreas in the Lobster, Homarus americanus

1967 ◽  
Vol 24 (11) ◽  
pp. 2339-2354 ◽  
Author(s):  
James E. Stewart ◽  
John W. Cornick ◽  
Diane M. Foley ◽  
M. F. Li ◽  
C. M. Bishop
Keyword(s):  
Serum Protein ◽  
Physiological Condition ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Experimental Conditions ◽  
Muscle Weight ◽  
Beef Liver ◽  
The Relationship

Total serum protein values, hemocyte numbers, and muscle weights were determined for 216 intermolt lobsters immediately after their capture, and for 230 others held captive under a variety of dietary and environmental conditions. Average muscle values ranged from approximately 13% to the more normal 20–25% of the live animals' weight, depending upon experimental conditions. The total serum protein up to a level of 55 mg/ml was shown to be a reliable indicator of muscle weights, although the relationship was not identical for all lobster groups. It appeared to be modified chiefly by the areas from which the different groups were taken. Diet was more important than the temperatures (5 to 14 C) in affecting changes in muscle and serum protein values. Starvation caused a greater reduction (50 to 70%) in the size of the hepatopancreas than in the muscle. Histological examination of the hepatopancreatic tissue showed that the lipid content was markedly reduced upon starvation and that a degeneration of this organ was apparent for lobsters fed a beef liver and herring diet. Measurement of serum proteins would appear to be a useful technique in experiments on lobster nutrition and have value, within specified limits, for assessing the physiological condition of wild lobsters.

Floral induction in date palm seedlings (Phoenix dactylifera var. Deglet Nour) cultured in vitro

1987 ◽  
Vol 65 (1) ◽  
pp. 137-142 ◽  
Author(s):  
Saïda Ammar ◽  
Abdellatif Benbadis ◽  
Bal Krishna Tripathi
Keyword(s):  
Date Palm ◽  
Root Formation ◽  
Early Stage ◽  
Floral Induction ◽  
Phoenix Dactylifera ◽  
Flower Bud ◽  
Precocious Flowering ◽  
Cultural Conditions ◽  
Bud Initiation

Flower bud initiation in 5-month-old seedlings of date palm (Phoenix dactylifera L. var. Deglet Nour) was studied under controlled conditions. Normally inflorescence formation in mature plants takes 8 to 10 years. In juvenile plants inflorescence formation was induced in a 16-h day at 28 °C, by a combination of 6-benzylaminopurine, indoleacetic acid, and glucose or sucrose. The present investigation has determined favourable cultural conditions for floral induction in date palm in vitro at a very early stage of ontogeny. Both male and female flowers were induced on young plants. Floral induction usually occurred only when root formation was completely inhibited. The apparent antagonism between root formation and floral development suggests a possible competition in the young plant for growth substances, although production of floral inhibitory substances from the root cannot be precluded. These observations on the induction of precocious flowering in date palm seedlings suggest a model of development, corresponding to neoteny, of this tree as an herb.

scholarly journals Potency of Ethanol Extracts Palm Seeds (Phoenix dactylifera L.) as Antidiabetic with Inhibition Kinetics Parameter

2020 ◽  
Vol 6 (2) ◽  
pp. 1-10
Author(s):  
Lisa Giovanny ◽  
Faliha Arinda Lestari ◽  
Nurul Marfira ◽  
Laksmi Ambarsari ◽  
Siti Warnasih
Keyword(s):  
Inhibitory Activity ◽  
Date Palm ◽  
Ethanol Extract ◽  
Phoenix Dactylifera ◽  
Total Phenolic ◽  
Phoenix Dactylifera L ◽  
Palm Juice ◽  
Ethanol Extracts ◽  
Fraction Measurement ◽  
Phenolic And Flavonoid

Dates seeds are one of the byproducts of the date palm juice industry which has the potential for further processing. Dates seeds contain active compounds that are known to have ability to inhibit αglucosidase. This study aims to measure the activity of 70% ethanol extract and the fraction of date palm seeds with a mechanism in inhibiting αglucosidase. The measurement of inhibitory activity was carried out by extracting date palm seeds using 70% water and ethanol. The extraction product is then fractionated with n-hexane, ethyl acetate, n-butanol, and water. Then the total phenolic and flavonoid calculations were calculated for each extract and fraction. Measurement of α-glucosidase inhibition activity was measured based on reaction of substrate to enzyme by the addition of inhibitors. The results showed that the ethanolextract had the highest total phenolic and flavonoid, which were 76.86 mg GAE / g and 21.19 mg QE / g, respectively. n-hexane fraction have the best inhibitory activity seen from the IC50 value, which is 12.69 mg/L. The kinetics of inhibition of 70% ethanol extract of date palm seeds are mixed inhibition. Keywords: α-glucosidase, date seeds, flavonoids, kinetic inhibition, phenolics

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