scholarly journals Antinociceptive Effect of an Aqueous Extract and Essential Oil from Baccharis heterophylla

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 116
Author(s):  
Erika Castillejos-Ramírez ◽  
Araceli Pérez-Vásquez ◽  
Rafael Torres-Colín ◽  
Andrés Navarrete ◽  
Adolfo Andrade-Cetto ◽  
...  
Keyword(s):  
Essential Oil ◽  
Chlorogenic Acid ◽  
Aqueous Extract ◽  
Solid Phase ◽  
Antinociceptive Effect ◽  
Acid Methyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Second Phase ◽  
Active Components ◽  
Caffeoylquinic Acid

Infusions and poultices prepared from the aerial parts of Baccharis heterophylla Kunth (Asteraceae) are widely used in Oaxaca (Mexico) for relieving painful and inflammatory complaints. Therefore, the antinociceptive potential of an aqueous extract (31.6–316 mg/kg, p.o.) and essential oil (30–177 µg/paw, i.pl.) of the plant was assessed using the formalin test. Both preparations inhibited the formalin-induced nociception response (100–316 mg/kg and 100–177 µg/paw, respectively) during the test’s second phase. Chemical analysis of the aqueous extract revealed that the major active components were chlorogenic acid (1), 3,4-di-O-(E)-caffeoylquinic acid (2), 3,5-di-O-(E)-caffeoylquinic acid (3), 4,5-di-O-(E)-caffeoylquinic acid (4), 3,5-di-O-(E)-caffeoylquinic acid methyl ester (5), apigenin (6), genkwanin (7), acacetin (8). Compounds 1–5 and 8 are new for B. heterophylla. A high-pressure liquid chromatographic method for quantifying chlorogenic acid (1) and di-caffeoylquinic acids 2–4 in the plant was developed and validated. Analyses of the essential oil and the headspace solid-phase microextraction products, via gas-chromatography-mass spectrometry, revealed that the major volatiles were β-pinene, myrcene, D-limonene, β-caryophyllene, and α-caryophyllene, which have demonstrated antinociceptive properties.

scholarly journals Chemical composition and antinociceptive activity of volatile fractions of the aerial parts of Solidago chilensis (Compositae)

Rodriguésia ◽  
2020 ◽  
Vol 71 ◽  
Author(s):  
Simone Sacramento Valverde ◽  
Stefânia Priscilla de Souza ◽  
Temistocles Barroso de Oliveira ◽  
Andressa Maia Kelly ◽  
Nathalia Ferreira Costa ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Antinociceptive Effect ◽  
Antinociceptive Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Aerial Parts ◽  
Germacrene D ◽  
Solidago Chilensis

Abstract Thirty-six compounds were identified from aerial parts of Solidago chilensis cultivated at PAF/FIOCRUZ campus in Rio de Janeiro city (RJ) using solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) analysis. These compounds are mainly non-oxygenated mono and sesquiterpenes. Germacrene D is the major compound of two the essential oil analyzed samples (12 to 23%) and of two of the volatiles samples analyzed by SPME (central stems and dry inflorescences samples). Limonene is the major compound in the fresh inflorescences sample (about 21%). The bornil acetate is present in both volatile fractions (16%) and essential oils (7-8%). All the essential oils samples evaluated shown a great antinociceptive activity, considering the used dose of the samples (1 mg/kg) and the standard substance (50 mg/kg). Solidago chilensis is one of Brazil arnicas and it is a medicinal plant widely used by the brazilian population. Other plants of the genus Solidago are also used in traditional medicine in North America and Europe. This is the first report of SPME analysis from Solidago genus and of the antinociceptive effect from Solidago chilensis essential oil.

scholarly journals Identification of VOCs in essential oils extracted using ultrasound- and microwave-assisted methods from sweet cherry flower

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Huimin Zhang ◽  
Hongguang Yan ◽  
Quan Li ◽  
Hui Lin ◽  
Xiaopeng Wen
Keyword(s):  
Essential Oil ◽  
Sweet Cherry ◽  
Dimethyl Sulfide ◽  
Solid Phase ◽  
Oil Yield ◽  
Gas Chromatography Mass Spectrometry ◽  
Process Conditions ◽  
Important Indicator ◽  
Microwave Assisted ◽  
Essential Oil Yield

AbstractThe floral fragrance of plants is an important indicator in their evaluation. The aroma of sweet cherry flowers is mainly derived from their essential oil. In this study, based on the results of a single-factor experiment, a Box–Behnken design was adopted for ultrasound- and microwave-assisted extraction of essential oil from sweet cherry flowers of the Brooks cultivar. With the objective of extracting the maximum essential oil yield (w/w), the optimal extraction process conditions were a liquid–solid ratio of 52 mL g−1, an extraction time of 27 min, and a microwave power of 435 W. The essential oil yield was 1.23%, which was close to the theoretical prediction. The volatile organic compounds (VOCs) of the sweet cherry flowers of four cultivars (Brooks, Black Pearl, Tieton and Summit) were identified via headspace solid phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS). The results showed that a total of 155 VOCs were identified and classified in the essential oil from sweet cherry flowers of four cultivars, 65 of which were shared among the cultivars. The highest contents of VOCs were aldehydes, alcohols, ketones and esters. Ethanol, linalool, lilac alcohol, acetaldehyde, (E)-2-hexenal, benzaldehyde and dimethyl sulfide were the major volatiles, which were mainly responsible for the characteristic aroma of sweet cherry flowers. It was concluded that the VOCs of sweet cherry flowers were qualitatively similar; however, relative content differences were observed in the four cultivars. This study provides a theoretical basis for the metabolism and regulation of the VOCs of sweet cherry flowers.

scholarly journals Characterization of Phenolic Constituents from Prunus cerasifera Ldb Leaves

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Wei Liu ◽  
Muhammad Farrukh Nisar ◽  
Chunpeng Wan
Keyword(s):  
Methyl Ester ◽  
Ethyl Acetate ◽  
Chlorogenic Acid ◽  
Acid Methyl Ester ◽  
Chemical Compositions ◽  
Preparative Hplc ◽  
Caffeoylquinic Acid ◽  
Prunus Cerasifera ◽  
Acid Methyl ◽  
Phenolic Constituents

To elucidate the chemical compositions of Prunus cerasifera Ldb leaves, the methanol extracts were firstly fractionated by ethyl acetate and n-butanol, respectively. The phenolic acid-rich fractions (ethyl acetate extracts) were further isolated by various chromatographic columns (CC) including MCI macroporous resin, normal-phase silica gel, Sephadex gel LH-20, octadecyl silane (ODS), and preparative HPLC to yield the phenolic compounds. The isolated compounds were analyzed by 1H-nuclear magnetic resonance (1H-NMR), 13C-NMR, and electrospray ionization mass spectral (ESI-MS) spectroscopy. Eleven phenolic acids were identified as p-coumaric acid (1), caffeic acid (2), ferulic acid (3), chlorogenic acid (4), 3-O-caffeoylquinic acid (5), 5-O-coumaroylquinic acid (6), 3-O-caffeoylquinic acid methyl ester (7), chlorogenic acid methyl ester (8), 3-O-caffeoyl-5-O-coumaroylquinic acid or 3-O-coumaroyl-5-O-caffeoylquinic acid (9), gallic acid (10), and protocatechuic acid (11). The current study pioneers to identify and report all the phenolic constituents from P. cerasifera Ldb leaves.

scholarly journals Comparison of Three Extraction Techniques for the Determination of Volatile Flavor Components in Broccoli

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 398
Author(s):  
Martyna Natalia Wieczorek ◽  
Małgorzata Majcher ◽  
Henryk Jeleń
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solid Phase ◽  
Food Product ◽  
Gas Chromatography Mass Spectrometry ◽  
Extraction Techniques ◽  
Active Components ◽  
Flight Mass Spectrometry ◽  
Full Profile ◽  
Simultaneous Distillation Extraction

To analyze aroma active components in a food product, the crucial step is to select a suitable extraction technique. It should provide isolation of all components responsible for aroma creation, without the formation of any artifacts during the procedure. Preferably, the extraction method should yield analyzed compounds in detectable levels. The presented study aimed to compare three popular extraction techniques used in flavor studies: solid-phase microextraction (SPME), solvent-assisted flavor evaporation (SAFE), and simultaneous distillation extraction (SDE) in order to isolate aroma components from broccoli (Brassica oleracea L. var. italica). Obtained extracts were analyzed by gas chromatography-olfactmetry (GC-O) to determine compounds with aroma activity as well as gas chromatography-mass spectrometry (GC-MS) and comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-ToFMS) to identify them. Thirty-four aroma active compounds were detected in broccoli by the applied techniques. SPME and SAFE together gave the full profile of aroma active components on chromatograms from GC-O, without artifacts that occurred in the SDE extract. SPME was particularly useful in the identification of early eluting compounds, while SAFE enabled isolating compounds with relatively low partition coefficients. Despite all the disadvantages of the SDE method, it leads to the identification of pyrazines, which were important contributors to the overall aroma.

Comparative Studies of Chemical Constituents of Hibiscus rosa-sinensis Using Different Extraction Techniques

2020 ◽  
Vol 981 ◽  
pp. 296-301
Author(s):  
Nur Salsabila Ahmad Roslan ◽  
Seema Zareen ◽  
Normaiza Zamri
Keyword(s):  
Essential Oil ◽  
Bioactive Compounds ◽  
Capillary Column ◽  
Chemical Constituents ◽  
Acid Methyl Ester ◽  
Gas Chromatography Mass Spectrometry ◽  
Hibiscus Rosa Sinensis ◽  
Soxhlet Apparatus ◽  
The Family

Hibiscus rosa-sinensis which belongs to the family Malvaceae, is a national flower of Malaysia. This glabrous tree can usually grow around 1 to 3 meters tall. The objective of present study was to categorize the essential oil constituent of flowers and leaves extract using different extraction method namely Soxhlet and hydrodistillation. The essential oil obtained from Soxhlet (10.75%) and hydrodistillation (11.40%) were subjected to Gas Chromatography-Mass Spectrometry (GC-MS) for determination of bioactive compounds. The GC-MS analysis was carried out by Agilent 7980A series GC instrument and DB-1MS capillary column with the dimensions of 30 m × 0.25 mm. A total of 21 and 20 compounds were identified for Soxhlet and hyrodistillation technique, respectively. Major compound found in essential oil for Soxhlet apparatus method was (Z,Z,Z)-9-12,15-Octadecatrienoic acid, methyl ester (23.59%) while for hydrodistillation was 1-Methylene-2b-hydroxymethyl-3,3-dimethyl-4b-(3-methylbut-2-enyl)-cyclohexane (16.57%). The presence of numerous bioactive compounds provides insights to the potential of the Hibiscus rosa-sinensis in pharmaceutical industry.

scholarly journals New Chemovariety of Lippia alba From Colombia: Compositional Analysis of the Volatile Secondary Metabolites and Some in vitro Biological Activities of the Essential Oil From Plant Leaves

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1986290
Author(s):  
Amner Muñoz-Acevedo ◽  
María C. González ◽  
Juan D. Rodríguez ◽  
Yurina Sh. De Moya
Keyword(s):  
Secondary Metabolites ◽  
Essential Oil ◽  
Solid Phase ◽  
Biological Activities ◽  
Biological Properties ◽  
Northern Region ◽  
Chemical Diversity ◽  
Gas Chromatography Mass Spectrometry ◽  
Lippia Alba

Lippia alba is a plant widely studied due to both chemical diversity and bioactivities related to its ethnobotanical uses. In this work, the composition of the volatile secondary metabolites (volatile fractions/essential oil, EO) of the flower/leaves of L. alba (from northern region of Colombia) was determined by solid phase micro-extraction/distillation-solvent extraction/microwave-hydrodistillation/gas chromatography-mass spectrometry (MWHD/GC-MS), along with some in vitro biological properties (cytotoxicity and acetylcholinesterase enzyme [AChe] inhibition) from leaf EO. Outstanding results were found: (i) cis-piperitone oxide (~13%-46%), germacrene D (~11%-30%), and limonene (~10%-22%) characterized the volatile secondary metabolites from different parts of the plant; (ii) leaf EO showed a moderate hemolytic activity (HC50: 580 ± 1 µg/mL), a significant cytotoxicity on lymphocytes (LC50: 127 ± 3 µg/mL), a high cytotoxicity on HEp2 cell line (LC50: 38 ± 2 µg/mL), and a moderate inhibitory effect on AChE (IC50: 28 ± 2 µg/mL). Based on these results, a new chemovar of L. alba is reported (represented by cis-piperitone oxide) along with its promising cytotoxic and AChE inhibiting properties.

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