scholarly journals Characterization of Phenolic Constituents from Prunus cerasifera Ldb Leaves

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Wei Liu ◽  
Muhammad Farrukh Nisar ◽  
Chunpeng Wan
Keyword(s):  
Methyl Ester ◽  
Ethyl Acetate ◽  
Chlorogenic Acid ◽  
Acid Methyl Ester ◽  
Chemical Compositions ◽  
Preparative Hplc ◽  
Caffeoylquinic Acid ◽  
Prunus Cerasifera ◽  
Acid Methyl ◽  
Phenolic Constituents

To elucidate the chemical compositions of Prunus cerasifera Ldb leaves, the methanol extracts were firstly fractionated by ethyl acetate and n-butanol, respectively. The phenolic acid-rich fractions (ethyl acetate extracts) were further isolated by various chromatographic columns (CC) including MCI macroporous resin, normal-phase silica gel, Sephadex gel LH-20, octadecyl silane (ODS), and preparative HPLC to yield the phenolic compounds. The isolated compounds were analyzed by 1H-nuclear magnetic resonance (1H-NMR), 13C-NMR, and electrospray ionization mass spectral (ESI-MS) spectroscopy. Eleven phenolic acids were identified as p-coumaric acid (1), caffeic acid (2), ferulic acid (3), chlorogenic acid (4), 3-O-caffeoylquinic acid (5), 5-O-coumaroylquinic acid (6), 3-O-caffeoylquinic acid methyl ester (7), chlorogenic acid methyl ester (8), 3-O-caffeoyl-5-O-coumaroylquinic acid or 3-O-coumaroyl-5-O-caffeoylquinic acid (9), gallic acid (10), and protocatechuic acid (11). The current study pioneers to identify and report all the phenolic constituents from P. cerasifera Ldb leaves.

scholarly journals The Apoptosis-inducing Antiproliferative Activity and Quantitative Phytochemical Profiling of Polyphenol-rich Part of Leea Aequata L. Leaves

2021 ◽  
Author(s):  
Md. Golam Mostofa ◽  
A. S.M. Ali Reza ◽  
Zidan Khan ◽  
Toshifumi Tsukahara ◽  
AHM Khurshid Alam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Methyl Ester ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Methanolic Extract ◽  
Acid Methyl Ester ◽  
Chemical Compositions ◽  
Acid Methyl ◽  
Anti Cancer

Abstract Aim/Objectives: Oxidative stress (OS) caused by the accumulation of free radicals in the body triggers many life-threatening diseases, including cancer. Plants are the rich source of safe and effective medicinal compounds like antioxidants that can prevent OS by scavenging free radicals. The aim of this study was to explore the chemical compositions of the methanolic extract of Leea aequata L. leaves possessed the highest antioxidant and cytotoxic activities.Methods and Materials: The Leea aequata leaves (LAL) was extracted with 80% methanol and the crude methanolic extract of LAL (CME) was successively fractionated with petroleum ether, chloroform, ethyl acetate and finally with water to get petroleum ether (PEF), chloroform (CHF), ethyl acetate (EAF) and aqueous (AQF) fraction, respectively. The antioxidant activity was evaluated by the in vitro standard spectrophotometric methods. The MTT colorimetric method was used to measure cytotoxic activity. Apoptosis was measured using fluorescein annexin V-FITC/PI staining method. GC-MS was used to investigate phytochemical compositions.Results: Among the fractions, the highest phenolic and flavonoid-rich fraction was EAF, which contained 267.05± 8.53 mg of gallic acid equivalent (GAE) and 152.98±1.96 mg of catechin equivalent (CAE)/gm of dried extract, respectively. The EAF was found to be the highest antioxidant and reducing power activities. Also, EAF had the strongest free radical scavenging activity with IC50 values of 5.11, 36.54 and 38.96 µg/mL for DPPH, hydroxyl and lipid peroxide free radicals, respectively. Moreover, EAF significantly reduced the HeLa cell proliferation and it was due to the induction of apoptosis of HeLa cells. Furthermore, GC-MS data revealed that EAF contained 17 compounds. Methyl esters of long chain fatty acid were the major chemical constituents. Interestingly, hexadecanoic acid, methyl ester; 9-octadecenoic acid (Z)-, methyl ester; 9,12-octadecadienoic acid, methyl ester (Z, Z) and phenol, 2,4-bis(1,1-dimethylethyl) are known to be antioxidant and cytotoxic. Antioxidant and anti-cancer activity were significantly correlated with the total content of phenolics and flavonoids, suggest that the fractions could be the good source of antioxidant and anti-cancer agents.Conclusion: The EAF of Leea aequata has strong polyphenolic content compared to the other fractions. The cytotoxic and antioxidant activity of Leea aequata were due to the presence of different bioactive compounds.

scholarly journals Natural Products from Scorzonera aristata (Asteraceae)

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Manuela Jehle ◽  
Johanna Bano ◽  
Ernst P. Ellmerer ◽  
Christian Zidorn
Keyword(s):  
Natural Products ◽  
Methyl Ester ◽  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Acid Methyl Ester ◽  
Quinic Acid ◽  
The South ◽  
Caffeic Acid Derivatives ◽  
Aerial Parts ◽  
Acid Methyl

The aerial parts of Scorzonera aristata Ramond ex DC., collected in the South Tyrolean Dolomites, yielded the flavonoids quercetin 3- O-glucoside, rutin, and isoorientin, and the caffeic acid derivatives chlorogenic acid, 4,5-dicaffeoyl quinic acid, and 3,5-dicaffeoyl quinic acid. Sub-aerial parts contained caffeic acid methyl ester, 3,5-dicaffeoyl quinic acid, and the triterpenes 3α-hydroxyolean-5-ene, lupeol, and magnificol. Chemosystematic implications of the isolated compounds are discussed briefly.

scholarly journals Antioxidant Potential of Crude Methanol Leaf extract and Fraction of Mallotus oppositifolius

2021 ◽  
pp. 168-184
Author(s):  
Ifeanyi Peter Onyeka ◽  
Felix Ahamefule Onyegbule ◽  
Christopher Obodike Ezugwu ◽  
Chibueze Jeremiah Ike ◽  
Chidalu B. Ikeotuonye
Keyword(s):  
Methyl Ester ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Acid Methyl Ester ◽  
Ethyl Acetate Fraction ◽  
Antioxidant Potential ◽  
Acid Methyl

Mallotus oppositifolius (Geiseler) Mull. Arg.  (Euphorbiaceae) is a predominant edible shrub in Nigeria. It is locally used for treatment of common infections and wounds. This study evaluated the toxicity and antioxidant potential of the crude extract and fractions of M. oppositifolius using invitro 1,1-diphenyl-2-picrylhydrazine (DPPH) radical scavenging assay. The result showed abundance of flavonoids (28.21), tannins (17.72), alkaloids (7.79) and Saponins (0.23) in the crude extract of M. oppositifolius leaf extract. Also, the result showed that the LD50 is above 5000 mg/kg, therefore the crude extract is safe for consumption. The result further showed that the ethyl-acetate and butanol had significant (p<0.05) antioxidant activity when compared with the control at 125, 62.5 and 31.125 µg/ml respectively while at 250 and 500 µg/ml there was no significant different in the level of reduction of oxidation between the butanol and ethyl acetate when compared with the control. The result further showed that the butanol and ethyl-acetate fraction of M. oppositifolius had the best antioxidant potential among the fractions studied. The result of GCMS studies of ethyl-acetate fraction showed the presence of 7,10,13-eicosatrienoic acid methyl ester, korormicin, agosterol, phyllanthin, ricinoic acid, 2,4,6-decatrienoic acid 1a,2,5,5a,6,9,10,10a-octahydro-5,5a-dihydroxy-4-(hydroxymethyl)-1,1,7,9, limonin, com-pounds that have shown to possess strong antioxidant activities. Also, the GCMS result of butanol fraction showed the presence of seven compounds which include: ferulylhistamine, hippeastrine, 10,13-eicosadienoic acid methyl ester, ethyl 5,8,11,14-eicosatetraenoate, 6-hydroxy-powelline-N-nitroso-7-demethoxy-aldehyde, 6,7-epoxypregn-4-ene-9,11,18-triol-3,20-dione 11,18-diacetate and 5Alpha-androstane-3,17-dione 17-monooxime,  compounds which may serve as a new lead for the treatment of diseases associated with the oxidative stress.

Chlorogenic acid methyl ester exerts strong anti-inflammatory effectsviainhibiting the COX-2/NLRP3/NF-κB pathway

2018 ◽  
Vol 9 (12) ◽  
pp. 6155-6164 ◽  
Author(s):  
Lang Zhang ◽  
Ya Fan ◽  
Hanwen Su ◽  
Li Wu ◽  
Yuying Huang ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Chlorogenic Acid ◽  
Acid Methyl Ester ◽  
Acid Methyl ◽  
Cox 2 ◽  
Anti Inflammatory

In vivoandin vitrostudies show that chlorogenic acid methyl ester (CME) has been proven to be a potential nutraceutical for preventing inflammation.

Study on Cold Flow Properties of Waste Cooking Methyl Ester

2014 ◽  
Vol 881-883 ◽  
pp. 635-640
Author(s):  
Guang Wu ◽  
Jia Hui Shong ◽  
Shen Tao Xu ◽  
Yong Bin Lai ◽  
Xiu Chen ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Acid Methyl Ester ◽  
Chemical Compositions ◽  
Flow Properties ◽  
Cold Flow ◽  
Cold Flow Properties ◽  
Acid Methyl ◽  
Flow Improver

The chemical compositions of waste cooking methyl ester (WCME) were analyzed by GC-MS, and the cold flow properties of WCME were studied by cold filer plugging point tester and viscosity tester. Through blending with petrodiesel and adding cold flow improver (CFI), the cold flow properties of WCME were improved efficiently. The study shows that WCME was mainly composed of saturated fatty acid methyl ester (SFAME) and unsaturated fatty acid methyl ester (UFAME), and contents of SFAME and UFAME were 27.63 and 71.81 w%, respectively. The cold filer plugging point (CFPP) of WCME was 0 °C, and the viscosity of it was 4.41 mm2·s-1 at 40 °C. Blending with 0 petrodiesel (0PD) and-10 petrodiesel (-10PD) decreased the CFPP of WCME to-4 °C and-13 °C, respectively. Meanwhile with WCME blending ratio increasing the viscosities of blending oils increased at the same temperature, and the viscosities increased gradually when the temperature decreased. Treating with CFI could significantly reduce the CFPP of blending oils which were composed of WCME and petrodiesel. The viscosities of blending oils with adding CFI were slightly higher than those with out CFI.

scholarly journals Anti-mycobacterial assessment and characterization of 5-O-caffeoylquinic acid methyl ester and rutin from Pavetta crassipes

2016 ◽  
pp. 001-007
Author(s):  
Patricia Odumosu ◽  
William Lough ◽  
Davis Yakubu ◽  
Keith Thomas ◽  
Gemma Williamson ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Acid Methyl Ester ◽  
Caffeoylquinic Acid ◽  
Acid Methyl

scholarly journals Characterization of Chemical Composition of Bryophyllum pinnatum Leaf Ethyl Acetate Fraction

2021 ◽  
pp. 15-24
Author(s):  
E. S. Asiwe ◽  
C. U. Igwe ◽  
V. A. Onwuliri ◽  
K. M. E. Iheanacho ◽  
J. N. Iheanacho
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Methyl Ester ◽  
Ethyl Acetate ◽  
Acid Methyl Ester ◽  
Ethyl Acetate Fraction ◽  
Potential Candidate ◽  
Acid Methyl ◽  
Ms Analysis ◽  
Bryophyllum Pinnatum

Aims: To characterize the chemical composition of Bryophyllum pinnatum leaf ethyl acetate fraction. Methodology: Quantitative phytochemical composition was assessed using gas chromatography fitted with flame ionization detector (GC-FID), while chemical characterization was via gas chromatography-mass spectrophotometry (GC-MS) analysis. The mass spectra peaks were matched with those found in the National Institute of Standard and Technology (NIST) spectral database. Results: Results revealed a rich presence of proanthocyanin, rutin, quinine, flavan-3-ol, anthocyanin, lunamarin, sapogenin, phenol, flavonones, steroids, epicatechin, kaempferol, phytate, oxalate, resveratol, catechin, flavones, tannin, ribalinidine, naringin, and spartein in varying proportion in B. pinnatum leaves ethylacetate fractions. GC-MS analysis of the fractions revealed the presence of at least 50 constituents. The major constituents were Hexadecanoic acid, methyl ester (24.88%), 10,13-Octadecadienoic acid, methyl ester (29.69%), Tetracosanoic acid, methyl ester (7.84%), Methyl stearate (6.97%), cis-Methyl 11-eicosenoate (6.26%), Methyl 18-methylnonadecanoate (4.99%), Docosanoic acid, methyl ester (3.71%) and  4,7-Methano-1H-indene, octahydro- (2.43%). Conclusion: This rich array of chemical compounds presents B. pinnatum leaves as an important source of potential lead compounds with biological and pharmacological benefits and hence a potential candidate for drug discovery.

Reduce Cold Filter Plugging Point and Kinematic Viscosity of Cottonseed-Based Biodiesel Fuel

2014 ◽  
Vol 1033-1034 ◽  
pp. 129-132
Author(s):  
Yong Bin Lai ◽  
Yu Qi Zhang ◽  
Xiu Chen ◽  
Yin Nan Yuan ◽  
Ling Ling Cai ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Kinematic Viscosity ◽  
Volume Fraction ◽  
Acid Methyl Ester ◽  
Biodiesel Fuel ◽  
Chemical Compositions ◽  
Cold Filter Plugging Point ◽  
Acid Methyl

The chemical compositions, cold filter plugging point (CFPP) and kinematic viscosity of cottonseed methyl ester (CSME) are investigated. Through blending with-10 petrodiesel (-10PD) and treating with Flow Fit, the CFPP and kinematic viscosity of CSME are improved. The study shows that CSME is mainly composed of fatty acid methyl esters (FAME), and the contents of saturated fatty acid methyl ester (SFAME) and unsaturated fatty acid methyl ester (UFAME) were 27.69% and 71.65% respectively. The CFPP and kinematic viscosity (40 °C) of CSME are-1 °C and 4.63 mm2/s respectively. Blending with-10PD decreased the CFPP of CSME to-12 °C.With temperature decreasing, the kinematic viscosities of CSME and CSME/-10PD increase. The lower the temperature is, the more differenced the kinematic viscosities of CSME and CSME/-10PD are. Treating with less than 3% (volume fraction) of Flow Fit, the CFPP of CSME and CSME/-10PD decreased significantly.

scholarly journals Phenolic Constituents with Antioxidant and α-glucosidase Inhibitory Activities from Sugar Maple (Acer saccharum Marsh.) Fall Leaves

2019 ◽  
Author(s):  
Liangliang Yao ◽  
Suyou Zhu ◽  
Chuying Chen ◽  
Youxin Yang ◽  
Wenwen Peng ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Phenolic Acids ◽  
Sugar Maple ◽  
Antioxidant Activities ◽  
Chemical Compositions ◽  
Preparative Hplc ◽  
Phenolic Constituents ◽  
Inhibitory Activities ◽  
First Time ◽  
Alpha Glucosidase

To elucidate the chemical compositions of the sugar fall maple leaves, the methanol extracts were firstly fractionated by ethyl acetate and n-butanol respectively. The phenolic acids-rich fractions (ethyl acetate extracts) were further purified by various chromatographic columns including XAD macroporous resin, Sephadex LH-20, ODS and semi-preparative HPLC to yield the compounds. The isolated compounds were characterized by 1H-Nuclear Magnetic Resonance (1H-NMR), 13C-NMR, and high resolution electrospray ionisation mass spectral (HR-ESI-MS) spectroscopy. Twenty eight phenolics including fourteen flavonoids (1-14), five quinic acid derivatives (15-19), five galloyl tannins (20-24) and four other phenolic acids (25-28) were isolated and their structures were identified. The isolated compounds were evaluated for their antioxidant and &alpha;-glucosidase inhibitory activities. All of the phenolics constituents showed DPPH scavenging antioxidant activities. While, glycosides of quercetin and myricetin, galloyl tannins were showed promising &alpha;-glucosidase inhibitory activity. All of the compounds except 4, 11, 12 and 28 were isolated from sugar maple for the first time. Moreover, Compounds 9, 10, 14, 20, 21, 23, 25 and 26 were isolated from the Acer species for the first time.

scholarly journals Chemical Constituents of Pyrrosia calvata

2015 ◽  
Vol 10 (7) ◽  
pp. 1934578X1501000
Author(s):  
Yu-Jie Chen ◽  
Guo-Yong Xie ◽  
Guang-Kai Xu ◽  
Yi-Qun Dai ◽  
Lu Shi ◽  
...  
Keyword(s):  
Methyl Ester ◽  
Chemical Constituents ◽  
Acid Methyl Ester ◽  
Caffeoylquinic Acid ◽  
Acid Methyl ◽  
Dicaffeoylquinic Acid ◽  
Whole Plant ◽  
The Family ◽  
First Time ◽  
Flavanone Glycoside

A novel flavanone glycoside, 3′,5′,5,7-tetrahydroxy-6- C- β-D-glucopyranosyl-flavanone (1), along with 16 known compounds, ( R/ S)-eriodictyol-8- C- β-D-glucopyranoside (2), quercetin-3- O- α-D-rhamnosyl (1′”→3′”)- β-D-glucopyranoside (3), hemipholin (4), 4 β-carboxymethyl-(-)-epicatechin methyl ester (5), kaempferol (6), quercetin (7), mangiferin (8), chlorogenic acid (9), 1,5- O-dicaffeoylquinic acid (10), 3,5- O-dicaffeoylquinic acid (11), 3- O-caffeoylquinic acid methyl ester (12), 1- O-caffeoyl glycoside (13), 4- O- β-D-glucopyranosyl-caffeic acid (14), 3′- O-methyleplcatechin-7- O- β-D-glucopyranoside (15), hop-22(29)-en-30-ol (16) and diploptene (17), were isolated from the whole plant of Pyrrosia calvata (Backer) Ching. Among them, compounds 2, 3, 4, 10, 11, 13 and 14 were isolated from the family Polypodiaceae for the first time, and compound 5 has not been recorded previously from the genus Pyrrosia.

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