scholarly journals Characterization of Ex Vivo Nonmelanoma Skin Tissue Using Raman Spectroscopy

Photonics ◽  
2021 ◽  
Vol 8 (7) ◽  
pp. 282
Author(s):  
Hieu T. M. Nguyen ◽  
Yao Zhang ◽  
Austin J. Moy ◽  
Xu Feng ◽  
Katherine R. Sebastian ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Skin Cancer ◽  
Cell Carcinoma ◽  
Raman Spectra ◽  
Ex Vivo ◽  
Hair Follicles ◽  
Nonmelanoma Skin Cancer ◽  
Skin Tissue ◽  
Biophysical Model ◽  
Resection Margins

Raman spectroscopy has shown great potential in detecting nonmelanoma skin cancer accurately and quickly; however, little direct evidence exists on the sensitivity of measurements to the underlying anatomy. Here, we aimed to correlate Raman measurements directly to the underlying tissue anatomy. We acquired Raman spectra of ex vivo skin tissue from 25 patients undergoing Mohs surgery with a fiber probe. We utilized a previously developed biophysical model to extract key biomarkers in the skin from the Raman spectra. We then examined the correlations between the biomarkers and the major skin structures (including the dermis, sebaceous glands, hair follicles, fat, and two types of nonmelanoma skin cancer—basal cell carcinoma (BCC) and squamous cell carcinoma (SCC)). SCC had a significantly different concentration of keratin, collagen, and nucleic acid than normal structures, while ceramide differentiated BCC from normal structures. Our findings identified the key proteins, lipids, and nucleic acids that discriminate nonmelanoma tumors and healthy skin using Raman spectroscopy. These markers may be promising surgical guidance tools for detecting tumors in resection margins.

Discrimination of non-melanoma skin cancer and keratosis from normal skin tissue in vivo and ex vivo by Raman spectroscopy

2019 ◽  
Vol 100 ◽  
pp. 131-141 ◽  
Author(s):  
Ana Mara Ferreira Lima ◽  
Camila Ribeiro Daniel ◽  
Ricardo Scarparo Navarro ◽  
Benito Bodanese ◽  
Carlos Augusto Pasqualucci ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Skin Cancer ◽  
Ex Vivo ◽  
Normal Skin ◽  
Skin Tissue ◽  
Non Melanoma Skin Cancer ◽  
Melanoma Skin

scholarly journals Basal Cell Carcinoma in a Child

2011 ◽  
Vol 2011 ◽  
pp. 1-3 ◽  
Author(s):  
Samet Vasfi Kuvat ◽  
Zuhal Gücin ◽  
Barış Keklik ◽  
Gülzade Özyalvaçlı ◽  
Karaca Başaran
Keyword(s):  
Skin Cancer ◽  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Safety Margin ◽  
Nonmelanoma Skin Cancer ◽  
Pathologic Examination ◽  
Pigmented Lesion

Basal cell carcinoma is the most commonly seen nonmelanoma skin cancer which is rarely encountered in the childhood period. An 11-year old child was admitted to our clinic due to an erythematous and a slightly pigmented lesion with a3×4 cm diameter on his posterior scalp. Macroscopically, the lesion was excised with a 10 mm safety margin. Pathologic examination revealed a basal cell carcinoma. No symptoms or signs of a syndrome were observed both in the patient and his family.

scholarly journals Assessment of Skin Deep Layer Biochemical Profile Using Spatially Offset Raman Spectroscopy

2021 ◽  
Vol 11 (20) ◽  
pp. 9498
Author(s):  
Martha Z. Vardaki ◽  
Konstantinos Seretis ◽  
Georgios Gaitanis ◽  
Ioannis D. Bassukas ◽  
Nikolaos Kourkoumelis
Keyword(s):  
Raman Spectroscopy ◽  
Skin Cancer ◽  
Deep Layer ◽  
Signal To Noise Ratio ◽  
Skin Tissue ◽  
Raman Signal ◽  
Histopathological Analysis ◽  
Biochemical Profile ◽  
Skin Cancer Screening ◽  
Zero Offset

Skin cancer is currently the most common type of cancer with millions of cases diagnosed worldwide yearly. The current gold standard for clinical diagnosis of skin cancer is an invasive and relatively time-consuming procedure, consisting of visual examination followed by biopsy collection and histopathological analysis. Raman spectroscopy has been shown to efficiently aid the non-invasive diagnosis of skin cancer when probing the surface of the skin. In this study, we employ a recent development of Raman spectroscopy (Spatially Offset Raman Spectroscopy, SORS) which is able to look deeper in tissue and create a deep layer biochemical profile of the skin in areas where cancer lesions subtly evolve. After optimizing the measurement parameters on skin tissue phantoms, we then adopted SORS on human skin tissue from different anatomical areas to investigate the contribution of the different skin layers to the recorded Raman signal. Our results show that using a diffuse beam with zero offset to probe a sampling volume where the lesion is typically included (surface to epidermis-dermis junction), provides the optimum signal-to-noise ratio (SNR) and may be employed in future skin cancer screening applications.

Measurement of Utility in Nonmelanoma Skin Cancer

2008 ◽  
Vol 12 (3) ◽  
pp. 102-106 ◽  
Author(s):  
William Lear ◽  
Jennifer Ellen Akeroyd ◽  
Nicole Mittmann ◽  
Christian Murray
Keyword(s):  
Quality Of Life ◽  
Skin Cancer ◽  
Cell Carcinoma ◽  
Standard Gamble ◽  
Health Utility ◽  
Nonmelanoma Skin Cancer ◽  
Assess Quality ◽  
Standard Gamble Method ◽  
Utility Scores

Background: Quality of life is an important variable in assessing the impact of a condition on patients. The current literature shows a minimal effect of nonmelanoma skin cancer (NMSC) on patients' quality of life. This contrasts with our own experience. Given this disparity, we sought to perform an additional study in this area. Past studies have used multiattribute methods to assess quality of life. In contrast, the present study uses health utility methods, which rate a patient's quality of life from 0 to 1, with 1 representing perfect health. Methods: Forty-one patients were guided through two standardized scenarios using a standard gamble process with a trained interviewer. Health utility scores were determined for both basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) using various NMSC treatment modalities. Results: All patients had health utility scores of 0.99 or higher. The standard gamble method showed no statistically significant differences in health utility scores for any treatment scenario for BCC or SCC using raw data comparisons. However, a modified standard gamble approach showed significantly higher health utility scores for both BCC and SCC treated using surgical modalities. Conclusion: Using the standard gamble health utility method in patients with BCC or SCC, it appears that these tumors have a minimal impact on the quality of life in the present study group. However, the results may simply reflect the poor sensitivity of the standard gamble health utility method to accurately assess quality of life changes in patients with NMSC. A modification of the standard gamble method did show that patients with NMSC associated surgical treatments with a better health outcome. New outcome measures need to be devised to accurately assess the toll of NMSC on patients.

scholarly journals Evaluation of tongue squamous cell carcinoma resection margins using ex-vivo MR

2017 ◽  
Vol 12 (5) ◽  
pp. 821-828 ◽  
Author(s):  
Stefan C. A. Steens ◽  
Elise M. Bekers ◽  
Willem L. J. Weijs ◽  
Geert J. S. Litjens ◽  
Andor Veltien ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Ex Vivo ◽  
Tongue Squamous Cell Carcinoma ◽  
Resection Margins

scholarly journals Nonmelanoma Skin Cancer in Childhood and Young Adult Cancer Survivors Previously Treated With Radiotherapy

2019 ◽  
Vol 17 (3) ◽  
pp. 237-243 ◽  
Author(s):  
Stefanie L. Thorsness ◽  
Azael Freites-Martinez ◽  
Michael A. Marchetti ◽  
Cristian Navarrete-Dechent ◽  
Mario E. Lacouture ◽  
...  
Keyword(s):  
Skin Cancer ◽  
General Population ◽  
Cell Carcinoma ◽  
Nonmelanoma Skin Cancer ◽  
Cancer Center ◽  
Young Adult Cancer Survivors ◽  
Common Diagnosis ◽  
Adult Cancer Survivors ◽  
The Mean

Background: Radiotherapy (RT) is a risk factor for nonmelanoma skin cancer (NMSC), specifically basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), but whether features, histology, or recurrence of NMSC after RT resemble those observed in the general population is unknown.Methods: A retrospective review (1994–2017) was performed within the Adult Long-Term Follow-Up Program and Dermatology Service at Memorial Sloan Kettering Cancer Center. Demographics, clinical features, histology, treatment, and recurrence were collected for this patient cohort that was under close medical surveillance. Pathology images were reviewed when available.Results: A total of 946 survivors (mean age, 40 years [SD, 13]) were assessed for NMSC. The mean age at first cancer diagnosis was 16 years (range, 0–40 years [11]), and the most common diagnosis was Hodgkin lymphoma (34%; n=318). In 63 survivors, 281 primary in-field lesions occurred, of which 273 (97%) were BCC and 8 (3%) were SCC. Mean intervals from time of RT to BCC and SCC diagnosis were 24 years (range, 2–44 years) and 32 years (range, 14–46 years), respectively. The most common clinical presentation of BCC was macule (47%; n=67), and the most common histologic subtypes were superficial for BCC (48%; n=131) and in situ for SCC (55%; n=5). Mohs surgery predominated therapeutically (42%; n=117), the mean duration of follow-up after treatment was 6 years (range, 12 days–23 years), and the 5-year recurrence rate was 1% (n=1).Conclusions: Most NMSCs arising in sites of prior RT were of low-risk subtypes. Recurrence was similar to that observed in the general population. Current guidelines recommend surgical intervention for tumors arising in sites of prior RT because they are considered to be at high risk for recurrence. These findings suggest that an expanded role for less aggressive therapy may be appropriate, but further research is needed.

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