scholarly journals Optimization of a siRNA Carrier Modified with a pH-Sensitive Cationic Lipid and a Cyclic RGD Peptide for Efficiently Targeting Tumor Endothelial Cells

Pharmaceutics ◽  
2015 ◽  
Vol 7 (3) ◽  
pp. 320-333 ◽  
Author(s):  
Tomoya Hada ◽  
Yu Sakurai ◽  
Hideyoshi Harashima
Keyword(s):  
Endothelial Cells ◽  
Cationic Lipid ◽  
Rgd Peptide ◽  
Ph Sensitive ◽  
Tumor Endothelial Cells

Modulation of calcium homeostasis in cultured rat aortic endothelial cells by intracellular acidification

1993 ◽  
Vol 265 (4) ◽  
pp. H1424-H1433 ◽  
Author(s):  
R. C. Ziegelstein ◽  
L. Cheng ◽  
P. S. Blank ◽  
H. A. Spurgeon ◽  
E. G. Lakatta ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Cytosolic Calcium ◽  
Ph Sensitive ◽  
Vascular Endothelial ◽  
Intracellular Acidification ◽  
Cytosolic Ph ◽  
Aortic Endothelial Cells ◽  
Endothelial Monolayers ◽  
Aortic Endothelial

Acidosis produces vasodilation in a process that may involve the vascular endothelium. Because synthesis and release of endothelium-derived vasodilatory substances are linked to an increase in cytosolic calcium concentration ([Ca2+]i), we examined the effect of intracellular acidification on cultured rat aortic endothelial cells loaded either with the pH-sensitive probe carboxy-seminaphthorhodafluor-1 or the Ca(2+)-sensitive fluorescent probe indo 1. The basal cytosolic pH (pHi) of endothelial monolayers in a 5% CO2-HCO3- buffer was 7.27 +/- 0.02 and that in a bicarbonate-free solution was 7.22 +/- 0.03. Acidification was induced either by removal of NH4Cl (delta pHi = -0.10 +/- 0.02), changing from a bicarbonate-free to a 5% CO2-HCO3(-)-buffered solution at constant buffer pH (delta pHi = -0.18 +/- 0.03), or changing from a 5% to a 20% CO2-HCO3- solution (delta pHi = -0.27 +/- 0.07). Regardless of the method used, intracellular acidification increased [Ca2+]i as indexed by indo 1 fluorescence. The increase in [Ca2+]i induced by changing from a 5 to a 20% CO2-HCO3- solution was not significantly altered by removal of buffer Ca2+ either before or after depletion of bradykinin- and thapsigargin-sensitive intracellular Ca2+ stores. Thus intracellular acidification of vascular endothelial cells releases Ca2+ into the cytosol either from pH-sensitive intracellular buffer sites, mitochondria, or from bradykinin- and thapsigargin-insensitive intracellular stores. This Ca2+ mobilization may be linked to endothelial synthesis and release of vasodilatory substances during acidosis.

scholarly journals Inhibition of Multidrug Transporter in Tumor Endothelial Cells Enhances Antiangiogenic Effects of Low-Dose Metronomic Paclitaxel

2015 ◽  
Vol 185 (2) ◽  
pp. 572-580 ◽  
Author(s):  
Kosuke Akiyama ◽  
Nako Maishi ◽  
Noritaka Ohga ◽  
Yasuhiro Hida ◽  
Yusuke Ohba ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Low Dose ◽  
Multidrug Transporter ◽  
Tumor Endothelial Cells

137 THE ROLE OF LYSYL OXIDASE ON PROANGIOGENIC PHENOTYPES OF TUMOR ENDOTHELIAL CELLS

2012 ◽  
Vol 187 (4S) ◽  
Author(s):  
Takahiro Osawa ◽  
Kyoko Hida ◽  
Noritaka Ohga ◽  
Yasuhiro Hida ◽  
Kazuko Kitayama ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Lysyl Oxidase ◽  
Tumor Endothelial Cells

scholarly journals Isolation of tumor endothelial cells from murine cancer

2019 ◽  
Vol 464 ◽  
pp. 105-113 ◽  
Author(s):  
Kazuhiro Taguchi ◽  
Takashi Onoe ◽  
Tomoaki Yoshida ◽  
Yoshinori Yamashita ◽  
Kiyomi Taniyama ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tumor Endothelial Cells

scholarly journals Two Modes of Toxicity of Lipid Nanoparticles Containing a pH-Sensitive Cationic Lipid on Human A375 and A375-SM Melanoma Cell Lines

BPB Reports ◽  
2019 ◽  
Vol 2 (4) ◽  
pp. 48-55
Author(s):  
Ahmed Y. AlBaloul ◽  
Yusuke Sato ◽  
Nako Maishi ◽  
Kyoko Hida ◽  
Hideyoshi Harashima
Keyword(s):  
Cell Lines ◽  
Melanoma Cell ◽  
Cationic Lipid ◽  
Lipid Nanoparticles ◽  
Ph Sensitive ◽  
Melanoma Cell Lines
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