scholarly journals Real-Time Translocation and Function of PKCβII Isoform in Response to Nociceptive Signaling via the TRPV1 Pain Receptor

2011 ◽  
Vol 4 (11) ◽  
pp. 1503-1517 ◽  
Author(s):  
Sravan Mandadi ◽  
Patricia J. Armati ◽  
Basil D. Roufogalis
Keyword(s):  
Real Time ◽  
And Function

Online Measurement of Glucose Consumption from HepG2 Cells Using an Integrated Bioreactor and Enzymatic Assay

2018 ◽  
Author(s):  
Anna Adams ◽  
Radha Krishna Murthy Bulusu ◽  
Nikita Mukhitov ◽  
Jose Mendoza-Cortes ◽  
Michael Roper
Keyword(s):  
Real Time ◽  
Glucose Concentration ◽  
Hepg2 Cells ◽  
Glucose Consumption ◽  
Microfluidic System ◽  
Structure And Function ◽  
Online Measurement ◽  
Fluorescent Assay ◽  
And Function ◽  
Integrated Bioreactor

In this work, we developed a microfluidic bioreactor for optimizing growth and maintaining structure and function of HepG2, and when desired, the device could be removed and the extracellular output from the bioreactor combined with enzymatic glucose reagents into a droplet-based microfluidic system. The intensity of the resulting fluorescent assay product in the droplets was measured, and was directly correlated to glucose concentration, allowing the effect of insulin on glucose consumption in the HepG2 cells to be observed and quantified online and in near real-time.

Online Measurement of Glucose Consumption from HepG2 Cells Using an Integrated Bioreactor and Enzymatic Assay

2018 ◽  
Author(s):  
Anna Adams ◽  
Radha Krishna Murthy Bulusu ◽  
Nikita Mukhitov ◽  
Jose Mendoza-Cortes ◽  
Michael Roper
Keyword(s):  
Real Time ◽  
Glucose Concentration ◽  
Hepg2 Cells ◽  
Glucose Consumption ◽  
Microfluidic System ◽  
Structure And Function ◽  
Online Measurement ◽  
Fluorescent Assay ◽  
And Function ◽  
Integrated Bioreactor

In this work, we developed a microfluidic bioreactor for optimizing growth and maintaining structure and function of HepG2, and when desired, the device could be removed and the extracellular output from the bioreactor combined with enzymatic glucose reagents into a droplet-based microfluidic system. The intensity of the resulting fluorescent assay product in the droplets was measured, and was directly correlated to glucose concentration, allowing the effect of insulin on glucose consumption in the HepG2 cells to be observed and quantified online and in near real-time.

W1363 Real Time Detection and Function of Melatonin Release from the Mucosa of Guinea Pig Ileum

2008 ◽  
Vol 134 (4) ◽  
pp. A-688 ◽  
Author(s):  
Bhavik A. Patel ◽  
Xiaochun Bian ◽  
James Galligan ◽  
Greg M. Swain
Keyword(s):  
Guinea Pig ◽  
Real Time ◽  
Guinea Pig Ileum ◽  
Real Time Detection ◽  
And Function

Assessment of left atrial volume and function in patients with psoriasis by using real time three-dimensional echocardiography

2015 ◽  
Vol 127 (21-22) ◽  
pp. 858-863 ◽  
Author(s):  
Halil Atas ◽  
Alper Kepez ◽  
Mehmet Bozbay ◽  
Dilek Seckin Gencosmanoglu ◽  
Altug Cincin ◽  
...  
Keyword(s):  
Real Time ◽  
Left Atrial ◽  
Three Dimensional ◽  
Left Atrial Volume ◽  
Atrial Volume ◽  
Dimensional Echocardiography ◽  
Three Dimensional Echocardiography ◽  
And Function

scholarly journals Characterization and Localization of Calb2 in Both the Testis and Ovary of the Japanese Flounder (Paralichthys olivaceus)

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1503
Author(s):  
Yuting Xiang ◽  
Yahui Wu ◽  
Haoran Zhang ◽  
Jikui Wu ◽  
Junling Zhang
Keyword(s):  
Real Time ◽  
Western Blotting ◽  
Real Time Pcr ◽  
Paralichthys Olivaceus ◽  
Neighboring Gene ◽  
Syntenic Relationship ◽  
Reading Frame ◽  
Cdna Sequences ◽  
Its Gene ◽  
And Function

Although its function in mammalian gonads has been gradually recognized, the expression and function of calretinin (CALB2)—a Ca2+-binding protein—in the testis and ovary of fish are still unclear. Here, we identified the cDNA sequences of calb2 in Paralichthys olivaceus (P. olivaceus); analyzed its gene structure and phylogenetic and syntenic relationship by bioinformatics; and investigated its tissue distribution and localization in the gonads by real-time PCR, western blotting, and immunohistochemistry. The P. olivaceuscalb2 gene has 11 exons and 10 introns, and the full-length cDNA is 1457 bp, including an open reading frame (ORF) of 816 bp encoding 271 amino acids. The CALB2 of P. olivaceus has a higher homology with Lates calcarifer (99%) compared with other species. The conserved synteny of calb2 neighboring gene loci was also detected in fish. Real-time PCR showed that the expression of calb2 mRNA is abundant not only in the brain, but also in the gonads, and exhibits a higher expression in the testis than in the ovary. Western blotting indicated that the CALB2 protein has a higher expression in the testis compared with the ovary. Immunohistochemistry demonstrated that the CALB2 protein appears in Leydig cells and the ovarian germ epithelium. These results reveal that calb2 plays an important role in the gonads of P. olivaceus.

Functional biomedical hydrogels for in vivo imaging

2016 ◽  
Vol 4 (48) ◽  
pp. 7793-7812 ◽  
Author(s):  
Kewen Lei ◽  
Qian Ma ◽  
Lin Yu ◽  
Jiandong Ding
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Structure And Function ◽  
Non Invasive ◽  
The Status ◽  
And Function

In vivo imaging of biomedical hydrogels enables real-time and non-invasive visualization of the status of structure and function of hydrogels.

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