Functional biomedical hydrogels for in vivo imaging

2016 ◽  
Vol 4 (48) ◽  
pp. 7793-7812 ◽  
Author(s):  
Kewen Lei ◽  
Qian Ma ◽  
Lin Yu ◽  
Jiandong Ding
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Structure And Function ◽  
Non Invasive ◽  
The Status ◽  
And Function

In vivo imaging of biomedical hydrogels enables real-time and non-invasive visualization of the status of structure and function of hydrogels.

Establishment of an mKate2-Expressing Cell Line for Non-Invasive Real-Time Breast Cancer In Vivo Imaging

2015 ◽  
Vol 17 (6) ◽  
pp. 811-818 ◽  
Author(s):  
Ivan Vuletic ◽  
Jinghao Liu ◽  
Honglian Wu ◽  
Yichen Ding ◽  
Yu Lei ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Real Time ◽  
In Vivo Imaging ◽  
Non Invasive

scholarly journals Fast widefield imaging of neuronal structure and function with optical sectioning in vivo

2020 ◽  
Vol 6 (19) ◽  
pp. eaaz3870 ◽  
Author(s):  
Ziwei Li ◽  
Qinrong Zhang ◽  
Shih-Wei Chou ◽  
Zachary Newman ◽  
Raphaël Turcotte ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
In Vivo Imaging ◽  
Neuromuscular Junctions ◽  
Structure And Function ◽  
Structured Illumination ◽  
Neuronal Structure ◽  
Optical Sectioning ◽  
Structured Illumination Microscopy ◽  
And Function

Optical microscopy, owing to its noninvasiveness and subcellular resolution, enables in vivo visualization of neuronal structure and function in the physiological context. Optical-sectioning structured illumination microscopy (OS-SIM) is a widefield fluorescence imaging technique that uses structured illumination patterns to encode in-focus structures and optically sections 3D samples. However, its application to in vivo imaging has been limited. In this study, we optimized OS-SIM for in vivo neural imaging. We modified OS-SIM reconstruction algorithms to improve signal-to-noise ratio and correct motion-induced artifacts in live samples. Incorporating an adaptive optics (AO) module to OS-SIM, we found that correcting sample-induced optical aberrations was essential for achieving accurate structural and functional characterizations in vivo. With AO OS-SIM, we demonstrated fast, high-resolution in vivo imaging with optical sectioning for structural imaging of mouse cortical neurons and zebrafish larval motor neurons, and functional imaging of quantal synaptic transmission at Drosophila larval neuromuscular junctions.

scholarly journals Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time

2020 ◽  
Vol 12 (12) ◽  
pp. 1123-1130
Author(s):  
Emily D. Cosco ◽  
Anthony L. Spearman ◽  
Shyam Ramakrishnan ◽  
Jakob G. P. Lingg ◽  
Mara Saccomano ◽  
...  
Keyword(s):  
Real Time ◽  
In Vivo Imaging ◽  
Non Invasive ◽  
Shortwave Infrared

scholarly journals In vivo imaging of photoreceptor structure and function in a non-human primate model of retinal degeneration

2017 ◽  
Vol 17 (7) ◽  
pp. 53
Author(s):  
Sarah Walters ◽  
Christina Schwarz ◽  
Robin Sharma ◽  
William S. Fischer ◽  
David DiLoreto ◽  
...  
Keyword(s):  
Retinal Degeneration ◽  
In Vivo Imaging ◽  
Structure And Function ◽  
Primate Model ◽  
And Function ◽  
Human Primate

Applications of phosphorescent materials for in-vivo imaging of brain structure and function

2016 ◽  
Author(s):  
Gregory Boverman ◽  
Xiaolei Shi ◽  
Victoria E. Cotero ◽  
Robert J. Filkins ◽  
Alok M. Srivastava ◽  
...  
Keyword(s):  
Brain Structure ◽  
In Vivo Imaging ◽  
Structure And Function ◽  
Brain Structure And Function ◽  
And Function

Osseointegration interface of calcified bone and endosseous implants

1992 ◽  
Vol 50 (2) ◽  
pp. 1096-1097
Author(s):  
K.E. Krizan ◽  
J.E. Laffoon ◽  
M.J. Buckley
Keyword(s):  
Structure And Function ◽  
Titanium Implants ◽  
En Bloc ◽  
Endosseous Implants ◽  
Ultrastructural Studies ◽  
The Past ◽  
Cacodylate Buffer ◽  
One Year ◽  
And Function

With increase use of tissue-integrated prostheses in recent years it is a goal to understand what is happening at the interface between haversion bone and bulk metal. This study uses electron microscopy (EM) techniques to establish parameters for osseointegration (structure and function between bone and nonload-carrying implants) in an animal model. In the past the interface has been evaluated extensively with light microscopy methods. Today researchers are using the EM for ultrastructural studies of the bone tissue and implant responses to an in vivo environment. Under general anesthesia nine adult mongrel dogs received three Brånemark (Nobelpharma) 3.75 × 7 mm titanium implants surgical placed in their left zygomatic arch. After a one year healing period the animals were injected with a routine bone marker (oxytetracycline), euthanized and perfused via aortic cannulation with 3% glutaraldehyde in 0.1M cacodylate buffer pH 7.2. Implants were retrieved en bloc, harvest radiographs made (Fig. 1), and routinely embedded in plastic. Tissue and implants were cut into 300 micron thick wafers, longitudinally to the implant with an Isomet saw and diamond wafering blade [Beuhler] until the center of the implant was reached.

Online Measurement of Glucose Consumption from HepG2 Cells Using an Integrated Bioreactor and Enzymatic Assay

2018 ◽  
Author(s):  
Anna Adams ◽  
Radha Krishna Murthy Bulusu ◽  
Nikita Mukhitov ◽  
Jose Mendoza-Cortes ◽  
Michael Roper
Keyword(s):  
Real Time ◽  
Glucose Concentration ◽  
Hepg2 Cells ◽  
Glucose Consumption ◽  
Microfluidic System ◽  
Structure And Function ◽  
Online Measurement ◽  
Fluorescent Assay ◽  
And Function ◽  
Integrated Bioreactor

In this work, we developed a microfluidic bioreactor for optimizing growth and maintaining structure and function of HepG2, and when desired, the device could be removed and the extracellular output from the bioreactor combined with enzymatic glucose reagents into a droplet-based microfluidic system. The intensity of the resulting fluorescent assay product in the droplets was measured, and was directly correlated to glucose concentration, allowing the effect of insulin on glucose consumption in the HepG2 cells to be observed and quantified online and in near real-time.

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