scholarly journals Development of a Rapid UHPLC-PDA Method for the Simultaneous Quantification of Flavonol Contents in Onions (Allium cepa L.)

2021 ◽  
Vol 14 (4) ◽  
pp. 310
Author(s):  
Ana V. González-de-Peredo ◽  
Mercedes Vázquez-Espinosa ◽  
Ceferino Carrera ◽  
Estrella Espada-Bellido ◽  
Marta Ferreiro-González ◽  
...  
Keyword(s):  
High Performance ◽  
Desirability Function ◽  
Radical Scavenging ◽  
Complete Separation ◽  
Raw Material ◽  
Antioxidant Compounds ◽  
Protective Effects ◽  
Quercetin Derivatives ◽  
Allium Cepa L ◽  
Successful Separation

Onion, one of the most consumed vegetables in the world, is also known to contain high levels of antioxidant compounds, with protective effects against different degenerative pathologies. Specifically, onion is rich in flavonols, mainly quercetin derivatives, which are compounds with high antioxidant and free radical scavenging power. For this reason, it is of the utmost importance to count on optimal analytical methods that allow for the determination and quantification of these compounds of interest. A rapid ultra-high performance liquid chromatography (UHPLC)-photo-diode array (PDA) method for the separation of the major flavonols in onions was developed using a Box–Behnken design in conjunction with multiresponse optimization on the basis of the desirability function. The conditions that provided a successful separation were 9.9% and 53.2% of phase B at the beginning and at the end of the gradient, respectively; 55 °C column working temperature; and 0.6 mL min−1 flow rate. The complete separation was achieved in less than 2.7 min with excellent chromatographic characteristics. The method was validated, and its high precision, low detection and quantification limits, good linearity, and robustness were confirmed. The correct applicability of the method improves the analysis of the raw material, increasing the quality of onions and its subproducts in terms of bioactive compounds and functional characteristics for consumers.

scholarly journals Optimized green extraction conditions of matcha green tea (Camellia sinensis) using central composite design for maximal polyphenol and antioxidant contents

BioResources ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 3255-3271
Author(s):  
Nor Hafiza Sayuti ◽  
Ammar Akram Kamarudin ◽  
Norazalina Saad ◽  
Nor Asma Ab. Razak ◽  
Norhaizan Mohd Esa
Keyword(s):  
Central Composite Design ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Desirability Function ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Green Extraction ◽  
Antioxidant Content ◽  
Optimum Extraction ◽  
Central Composite

Response surface methodology (RSM) was employed to optimize the extraction conditions of phenolic and antioxidant compounds from matcha green tea (Camellia sinensis) using central composite design (CCD). The desirability function was used to find the optimum extraction conditions. The highest polyphenol and antioxidant content yield were reached at a temperature of 80 °C, an extraction time of 20 min, a liquid-to-solid ratio of 100 mL/g, and a desirability value of 0.948. The experimental values for total phenolics under the optimum extraction conditions were 317.62 ± 3.45 mg GAE/g and 29.21 ± 0.38 mg RE/g for the total flavonoids. The antioxidant activity (AA) was evaluated using 2,2 diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), which showed radical scavenging activities at 88.28 ± 0.14% and 90.02 ± 0.14%, respectively. The high performance liquid chromatography (HPLC) analysis at the optimum condition revealed 14 compounds. Among the analyzed compounds, matcha green tea extract (MGTE) had the highest content of epigallocatechin gallate (EGCG) with 95.48 mg/g, followed by epicatechin gallate (ECG) at 74.48 mg/g, and catechin at 28.94 mg/g. The results suggested that the optimized parameters of heat-assisted extraction provide an ideal green extraction method for the extraction of the high polyphenol and antioxidant content in matcha green tea.

scholarly journals Synthesis, Characterization, and Antioxidant Activity of 8-Hydroxygenistein

2020 ◽  
Vol 15 (1) ◽  
pp. 1934578X2090139 ◽  
Author(s):  
Jin Shao ◽  
Tong Zhao ◽  
Hui-Ping Ma ◽  
Zheng-Ping Jia ◽  
Lin-Lin Jing
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Positive Control ◽  
Raw Material ◽  
Biochanin A ◽  
Total Antioxidant Activity ◽  
Nitric Oxide Radical ◽  
Total Antioxidant

It was reported that 8-hydroxygenistein (8-OHG) was synthesized by methylation, bromination, methoxylation, and demethylation using cheap and readily available biochanin A as raw material. All synthesized products were structurally confirmed by ultra-high-performance liquid chromatography (UHPLC), infrared spectroscopy, mass spectrometry, 1H-nuclear magnetic resonance (NMR), and 13C-NMR. In addition, we examined the antioxidant capacity of 8-OHG using 6 different methods such as 1,1-diphenyl-2-picrylhydrazyl radical scavenging, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) radical (ABTS) scavenging, nitric oxide radical (NO) scavenging, superoxide radical (O2 −•) scavenging, reducing power assay, and total antioxidant activity using ascorbic acid (VC) as a positive control. Compared with VC, 8-OHG exhibited higher total antioxidant activity and stronger scavenging activity on ABTS, NO, and O2 −•. These results indicate that 8-OHG is an excellent antioxidant agent and may be effective in preventing damage induced by free radical.

scholarly journals Antioxidant and Antityrosinase Activity ofFlemingia macrophyllaandGlycine tomentellaRoots

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Bor-Sen Wang ◽  
Lih-Jeng Juang ◽  
Jeng-Jer Yang ◽  
Li-Ying Chen ◽  
Huo-Mu Tai ◽  
...  
Keyword(s):  
High Performance ◽  
Radical Scavenging ◽  
Water Extract ◽  
Protective Effects ◽  
Tyrosinase Inhibitor ◽  
Bioactive Constituents ◽  
Chromatography Analysis ◽  
Glycine Tomentella ◽  
Phenolic Components ◽  
Antityrosinase Activity

The antioxidant and antityrosinase activities of the water extract ofFlemingia macrophyllaroot (WEFM) were investigated. The results showed that WEFM exhibited radical scavenging and reducing activities, as well as ferrous ion chelating property. In addition, WEFM also protected phospholipids against oxidation, indicating that WEFM could protect biomolecules from oxidative damage. Meanwhile, in the range of 50–100 μg/mL, the tyrosinase inhibitory activity of WEFM increased with an increase in sample concentration and was superior to that of the water extract ofGlycine tomentellaroot (WEGT). A high performance liquid chromatography analysis was used to determine the phenolic components, revealing that daidzin, daidzein, genistin, and genistein were present in WEFM and WEGT. Acting as an antioxidant and a tyrosinase inhibitor, these bioactive constituents could contribute to the protective effects of WEFM. Overall, the results showed that WEFM might serve as a natural antioxidant and tyrosinase inhibitor.

scholarly journals Comparison between Fermentation and Ultrasound-Assisted Extraction: Which Is the Most Efficient Method to Obtain Antioxidant Polyphenols from Sambucus nigra and Punicagranatum Fruits?

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 386
Author(s):  
Luana Beatriz dos Santos Nascimento ◽  
Antonella Gori ◽  
Ilaria Degano ◽  
Alessandro Mandoli ◽  
Francesco Ferrini ◽  
...  
Keyword(s):  
High Performance ◽  
Radical Scavenging ◽  
Punica Granatum ◽  
Sambucus Nigra ◽  
Ultrasound Assisted Extraction ◽  
Quercetin Derivatives ◽  
Antioxidant Capacities ◽  
Assisted Extraction ◽  
Pharmaceutical Industries ◽  
Ultrasound Assisted

Fruit extracts of Sambucus nigra L. (elderberry) and Punica granatum L. (pomegranate) have several applications in nutraceutical, cosmetics, and pharmaceutical industries thanks to their richness in antioxidant polyphenols, whose composition changes with the extraction method applied. We aimed to compare the efficiency of the fermentation extraction, recently applied by industries, with the ultrasound-assisted extraction–UAE, a well-known and efficient technique, on the yield of antioxidant polyphenols from elderberry fruits and pomegranate fruit-peels. Extracts were obtained by both methods, analyzed by high-performance liquid chromatography (HPLC) and the antioxidant capacities were evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Hydroxyl Radical Scavenging (HRS) assays. The main compounds detected in elderberry were caffeoyl and quercetin derivatives, present in higher amounts in UAE extracts. In pomegranate, punicalagin were the main constituents, also detected in higher contents in the UAE extracts compared to fermented ones. The UAE was more suitable for extracting anthocyanins from pomegranate. In addition, higher antioxidant capacities were observed in UAE extracts, possibly due to their richness in polyphenols. Therefore, despite the recent wide applicability and the good performance of the fermentation process, the UAE may be considered more efficient for the extraction of polyphenols from S. nigra and P. granatum fruits and may be used to obtain polyphenolic antioxidant extracts to be applied by several industries.

scholarly journals Determination of Antioxidant Compounds, Proximate Compositions and Assessment of Free Radical Scavenging Activities of Nypa Fruticans Wurmb. Sap

2021 ◽  
Vol 29 (3) ◽  
Author(s):  
Siti Fatimah Roqiah Yahaya ◽  
Niza Samsuddin ◽  
Suhana Mamat ◽  
Rozita Hod ◽  
Nor Zamzila Abdullah ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Nypa Fruticans ◽  
Ic50 Value ◽  
Nipa Palm

Nipa palm sap (NPS) as source of medicine traditionally used to treat various diseases. This study identified good radical scavenging activity in NPS with the IC50 value of 33.36 μg/mL using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. NPS comprises of moisture (72.44%), ash (1.04%), protein (7.04%), carbohydrate (19.48%), fat (0%), and energy level (106 kcal). Glucose (0.3%) and fructose (1.8%) were detected using high-performance liquid chromatography. Maleic acid, cinnamic acid, chlorogenic acid, and kaempferol were the predominant compounds revealed by ultra-high-performance liquid chromatography. Overall, NPS has the potential antioxidants sources with significant health benefits and values for commercialisation.

scholarly journals Development and Validation of HPLC-DAD Method for Simultaneous Determination of Seven Food Additives and Caffeine in Powdered Drinks

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1119
Author(s):  
Imanulkhan Imanulkhan ◽  
Widiastuti Setyaningsih ◽  
Abdul Rohman ◽  
Miguel Palma
Keyword(s):  
Simultaneous Determination ◽  
Phosphate Buffer ◽  
High Performance ◽  
Desirability Function ◽  
Food Additives ◽  
Complete Separation ◽  
Sodium Saccharin ◽  
Array Detection ◽  
Development And Validation

The usage of food additives must respect the general legislation in force in the country and requires a reliable analytical method for surveillance. This research aimed to develop a high-performance liquid chromatography with diode array detection (HPLC-DAD) method for the simultaneous determination of seven food additives and caffeine in powdered drinks. Three factors likely to affect the chromatographic separation, namely, mobile phase composition at the beginning (x1, 0–10% of the amount of methanol in the phosphate buffer) and the end (x2, 60–100% of the amount of methanol in the phosphate buffer) of the gradient program and pH (x3, 3–7), were evaluated with the aid of a Box–Behnken Design (BBD). Subsequently, multi-response optimizations for chromatographic resolutions (Rs) and analysis time were performed using the response surface methodology (RSM) in conjunction with the desirability function (DF). Complete separation (Rs > 1.5) of seven food additives and caffeine was achieved in less than 16 min by applying 8.5% methanol in the phosphate buffer at the beginning and 90% at the end of the gradient program, in pH 6.7. The developed method was validated with low limits of detection (ranging from 1.16 mg kg−1 (sodium saccharin) to 3.00 mg kg−1 (acesulfame potassium)), low limits of quantification (ranging from 3.86 mg kg−1 (sodium saccharin) to 10.02 mg kg−1 (acesulfame potassium)), high precision (CV < 4%), and high accuracy (recoveries from 95 to 101% at 80, 100, and 120% of the target concentration). The method was successfully used to assess the seven food additives and caffeine in commercially available powdered drinks.

scholarly journals Colon Bioaccessibility under In Vitro Gastrointestinal Digestion of Different Coffee Brews Chemically Profiled through UHPLC-Q-Orbitrap HRMS

Foods ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 179
Author(s):  
Luigi Castaldo ◽  
Luana Izzo ◽  
Alfonso Narváez ◽  
Yelko Rodríguez-Carrasco ◽  
Michela Grosso ◽  
...  
Keyword(s):  
High Performance ◽  
Coffee Consumption ◽  
Antioxidant Compounds ◽  
Protective Effects ◽  
Gastrointestinal Digestion ◽  
Orbitrap Mass Spectrometry ◽  
Health Related ◽  
Related Compounds ◽  
Coffee Brews

Coffee represents one of the most traditionally consumed beverages worldwide, containing a broad range of human health–related compounds. According to previous studies, regular coffee consumption may display protective effects against colorectal cancer and other chronic diseases. The main goal of this research was to evaluate the bioaccessibility of phenolic content and variation in antioxidant capacity of three different types of coffee brews after simulated gastrointestinal digestion. This would allow to elucidate how antioxidant compounds present in coffee may exert their effect on the human body, especially in the colonic stage. Moreover, the content of bioactive compounds namely chlorogenic acids (CGAs, n = 11) and caffeine was also assessed throughout ultra-high-performance liquid chromatography followed by high-resolution Orbitrap mass spectrometry (UHPLC-Q-Orbitrap HRMS). The three main isomers of caffeoylquinic acid constituted the highest fraction of CGAs present in the samples, accounting for 66.0% to 70.9% of total CGAs. The bioaccessibility of coffee polyphenols significantly increased in digested samples from 45.9% to 62.9% at the end of the colonic passage, compared to the non-digested samples. These results point to the colonic stage as the major biological site of action of the active antioxidant coffee compounds.

scholarly journals β-ecdysone content and antioxidant capacity in different organs of Brazilian ginseng

2021 ◽  
Vol 51 (5) ◽  
Author(s):  
Lucas Dutra Zani da Silva Souza ◽  
Stéphane Raquel Almeida Velande da Fonseca ◽  
Ariana Ferrari ◽  
Daniele Fernanda Felipe
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Dpph Radical ◽  
Brazilian Ginseng ◽  
Dpph Radical Scavenging ◽  
Β Carotene

ABSTRACT: Plants that contain antioxidant compounds have attracted increasing interest for their vital role in the attenuation of oxidative damage caused by free radicals and in the treatment of various diseases. The present study investigated the β-ecdysone content and the antioxidant activity of Brazilian ginseng (Pfaffia glomerata) extracts obtained from inflorescences, stems, and roots. The P. glomerata extracts were tested for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, β-carotene bleaching test, and phosphomolybdenum method. The β-ecdysone content of P. glomerata extracts was measured by high-performance liquid chromatography (HPLC). The P. glomerata inflorescences showed the strongest DPPH radical scavenging activity and the strongest antioxidant activity in the β-carotene bleaching assay and phosphomolybdenum test. The roots showed the lowest antioxidant capacity in all of the assays. The concentration of β-ecdysone in the plant organs followed the following decreasing order: inflorescences > stems > roots. The present study showed that P. glomerata inflorescence extract had high antioxidant capacity that could be attributed to the presence of β-ecdysone.

scholarly journals Cellular Antioxidant Activity of Olive Pomace Extracts: Impact of Gastrointestinal Digestion and Cyclodextrin Encapsulation

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5027
Author(s):  
Kristina Radić ◽  
Ivana Vinković Vrček ◽  
Ivan Pavičić ◽  
Dubravka Vitali Čepo
Keyword(s):  
Matrix Effects ◽  
Cell Damage ◽  
Radical Scavenging ◽  
Raw Material ◽  
Protective Effects ◽  
Olive Pomace ◽  
Gastrointestinal Digestion ◽  
Polyphenolic Extract ◽  
Tert Butylhydroperoxide ◽  
Pre Treatment

Olive pomace is a valuable secondary raw material rich in polyphenols, left behind after the production of olive oil. The present study investigated the protective effect of a polyphenolic extract from olive pomace (OPE) on cell viability and antioxidant defense of cultured human HepG2 cells submitted to oxidative stress induced by tert-butylhydroperoxide (tBOOH). The investigation considered possible matrix effects, impact of gastrointestinal digestion and cyclodextrin (CD) encapsulation. Pre-treatment of cells with OPE prevented cell damage and increased intracellular glutathione but did not affect the activity of glutathione peroxidase and superoxide dismutase. OPE matrix significantly enhanced cell protective effects of major antioxidants, such as hydroxytyrosol (HTS), while cyclodextrin encapsulation enhanced activity of OPE against intracellular reactive oxygen species (ROS) accumulation. The obtained results show that OPE is more potent antioxidant in comparison to equivalent dose of main polyphenols (HTS and TS) and that increasing solubility of OPE polyphenols by CD encapsulation or digestion enhances their potential to act as intracellular antioxidants. Antioxidative protection of cells by OPE was primarily achieved through direct radical-scavenging/reducing actions rather than activation of endogenous defense systems in the cell.

scholarly journals Potential antioxidant effects of Narcissus tazetta phenolic compounds against cadmium chloride induced hepatotoxicity in Swiss albino mice

2021 ◽  
Author(s):  
Saoussen Ben-Abdallah ◽  
Mediha Sefi ◽  
Nejla Soudani ◽  
Amel Hamdi ◽  
Safa Bejaoui ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Cadmium Chloride ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Protective Effects ◽  
Gamma Glutamyl Transpeptidase ◽  
Narcissus Tazetta ◽  
Protein Thiols

Abstract Narcissus tazetta (Amaryllidaceae) is a medicinal plant, widely used for cut flowers and potted ornamental plant in Tunisia flora. The current study evaluated the phenolic composition and antioxidant properties of its flower extracts and investigated its potential protective activity against cadmium chloride (CdCl2)-induced hepatotoxicity in mice. Adult Swiss mice were exposed either to CdCl2 (0.16 mg/kg bw) or to Narcissus tazetta extract (100 or mg/kg bw) or to their combination. Cadmium chloride and Narcissus tazetta flower extracts were given by intraperitoneal injection and gavage respectively for eight consecutive days. Results indicated that ethanolic flower extracts contained high levels of total phenolic and flavonoid contents along with a strong total antioxidant and 2,2-Diphenyl-1-picrylhydrazyl free radical scavenging activities. High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) analysis identified eight phenolic compounds, including rutin, Kaempferol glycosides and chlorogenic acids. The extract also exhibited marked hepato-protective effects against CdCl2 toxicity by reducing hepatic levels of malondialdehyde, advanced oxidation protein products, hydrogen peroxide, metallothioneins and DNA degradation. Additionally, co-administration of Narcissus tazetta flower extracts lowered the plasma activities of transaminases, gamma glutamyl transpeptidase and lactate dehydrogenase, and increased hepatic levels of reduced glutathione, non-protein thiols, vitamin C, and catalase activity. The hepatoprotective effects of the extract were demonstrated by histopathological improvement of liver disorders. The current study provided ethnopharmacological application of Narcissus tazetta flower extracts against CdCl2 induced oxidative stress, suggesting its chemoprevention role of its phenolic compounds as a natural antioxidant.

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