scholarly journals Antioxidant and Antityrosinase Activity ofFlemingia macrophyllaandGlycine tomentellaRoots

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Bor-Sen Wang ◽  
Lih-Jeng Juang ◽  
Jeng-Jer Yang ◽  
Li-Ying Chen ◽  
Huo-Mu Tai ◽  
...  
Keyword(s):  
High Performance ◽  
Radical Scavenging ◽  
Water Extract ◽  
Protective Effects ◽  
Tyrosinase Inhibitor ◽  
Bioactive Constituents ◽  
Chromatography Analysis ◽  
Glycine Tomentella ◽  
Phenolic Components ◽  
Antityrosinase Activity

The antioxidant and antityrosinase activities of the water extract ofFlemingia macrophyllaroot (WEFM) were investigated. The results showed that WEFM exhibited radical scavenging and reducing activities, as well as ferrous ion chelating property. In addition, WEFM also protected phospholipids against oxidation, indicating that WEFM could protect biomolecules from oxidative damage. Meanwhile, in the range of 50–100 μg/mL, the tyrosinase inhibitory activity of WEFM increased with an increase in sample concentration and was superior to that of the water extract ofGlycine tomentellaroot (WEGT). A high performance liquid chromatography analysis was used to determine the phenolic components, revealing that daidzin, daidzein, genistin, and genistein were present in WEFM and WEGT. Acting as an antioxidant and a tyrosinase inhibitor, these bioactive constituents could contribute to the protective effects of WEFM. Overall, the results showed that WEFM might serve as a natural antioxidant and tyrosinase inhibitor.

scholarly journals The Inhibitory Effects of Aqueous Extract from Guava Twigs,Psidium guajavaL., on Mutation and Oxidative Damage

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Zhi-Chyang Kang ◽  
Ming-Tsung Yen ◽  
Chih-Kwang Chiu ◽  
Horng-Cherng Wu ◽  
Shu-Ling Huang ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Aqueous Extract ◽  
High Performance ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Protective Effects ◽  
Inhibitory Effects ◽  
Phenolic Components ◽  
Phenolic Constituents

This study examines the inhibitory effects of the aqueous extract from guava twigs (GTE),Psidium guajavaL., on mutation and oxidative damage. The results show that GTE inhibits the mutagenicity of 4-nitroquinoline-N-oxide (4-NQO), a direct mutagen, and 2-aminoanthracene (2-AA), an indirect mutagen, towardSalmonella typhimuriumTA 98 and TA 100. In addition, GTE shows radical scavenging, reducing activities, tyrosinase inhibition, and liposome protection effects. Meanwhile, GTE in the range of 0.1–0.4 mg/mL protects liver cells fromtert-butyl-hydroperoxide-(t-BHP-) induced cytotoxicity. Furthermore, the cytotoxicity inhibition of GTE in thet-BHP-treated cells was demonstrated in a dose-dependent manner. High-performance liquid chromatography analysis suggests that the major phenolic constituents in GTE are gallic acid, ferulic acid, and myricetin. These active phenolic components may contribute to the biological protective effects of GTE in different models. The data suggest that GTE exhibiting biological activities can be applied to antimutation, antityrosinase, and antioxidative damage.

scholarly journals Development of a Rapid UHPLC-PDA Method for the Simultaneous Quantification of Flavonol Contents in Onions (Allium cepa L.)

2021 ◽  
Vol 14 (4) ◽  
pp. 310
Author(s):  
Ana V. González-de-Peredo ◽  
Mercedes Vázquez-Espinosa ◽  
Ceferino Carrera ◽  
Estrella Espada-Bellido ◽  
Marta Ferreiro-González ◽  
...  
Keyword(s):  
High Performance ◽  
Desirability Function ◽  
Radical Scavenging ◽  
Complete Separation ◽  
Raw Material ◽  
Antioxidant Compounds ◽  
Protective Effects ◽  
Quercetin Derivatives ◽  
Allium Cepa L ◽  
Successful Separation

Onion, one of the most consumed vegetables in the world, is also known to contain high levels of antioxidant compounds, with protective effects against different degenerative pathologies. Specifically, onion is rich in flavonols, mainly quercetin derivatives, which are compounds with high antioxidant and free radical scavenging power. For this reason, it is of the utmost importance to count on optimal analytical methods that allow for the determination and quantification of these compounds of interest. A rapid ultra-high performance liquid chromatography (UHPLC)-photo-diode array (PDA) method for the separation of the major flavonols in onions was developed using a Box–Behnken design in conjunction with multiresponse optimization on the basis of the desirability function. The conditions that provided a successful separation were 9.9% and 53.2% of phase B at the beginning and at the end of the gradient, respectively; 55 °C column working temperature; and 0.6 mL min−1 flow rate. The complete separation was achieved in less than 2.7 min with excellent chromatographic characteristics. The method was validated, and its high precision, low detection and quantification limits, good linearity, and robustness were confirmed. The correct applicability of the method improves the analysis of the raw material, increasing the quality of onions and its subproducts in terms of bioactive compounds and functional characteristics for consumers.

scholarly journals In vitro antioxidant, antiglycation, and MGO scavenging effects of Leonurus sibiricus water extract

2021 ◽  
pp. 84-95
Author(s):  
Xiu Qing Chong ◽  
Kirtani AP Anamalay ◽  
Okechukwu Patrick Nwabueze ◽  
Hor Kuan Chan
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Antioxidant Property ◽  
Future Research ◽  
Total Phenolic ◽  
Bioactive Constituents ◽  
Bioactive Component ◽  
Glycation End Products

Recently, extensive research has been conducted to evaluate the inhibitory activity of different plant species on the advanced glycation end products (AGEs). L. sibiricus is a traditional herb that has been used for postpartum confinement meals in Sarawak, Malaysia and pharmacologically possess anti-hemorrhagic, antioxidant, anti-diabetic, and anti-cancer. The aim of this research was to evaluate the antioxidant, anti-AGEs, and preliminary biochemical screening of bioactive component present in L. sibiricus in water extract. Free radical scavenging activity of L. sibiricus was evaluated via DPPH, hydroxyl radical, nitric oxide, lipid peroxidation, chelating capacity, and total phenolic content was evaluated comparing with gallic acid. Inhibition of formation AGEs by L. sibiricus was evaluated using BSA-MGO, BSA-glucose and MGO scavenging assays. Flavonoids, phenols, saponins, alkaloids, phytosterol, and diterpenoids were identified in L. sibiricus extract. It also seems to inhibit early and late formation of AGE and MGO scavenging ability. L. sibiricus was able to inhibit the formation of early and late formation of AGE through the scavenging of the formation of reactive dicarbonyl intermediates and reduce the formation of methylquinoxaline adducts through the scavenge of MGO. The inhibition of AGEs formation by L. sibiricus maybe due its antioxidant property and the presence phytochemical bioactive constituents which has been previously reported to possess antioxidant and anti-AGE activity. Future research is ongoing to identify the adducts formed because of MGO scavenging by L. sibiricus using HPLC.

scholarly journals The Health-Promoting Potential of Salix spp. Bark Polar Extracts: Key Insights on Phenolic Composition and In Vitro Bioactivity and Biocompatibility

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 609 ◽  
Author(s):  
Patrícia A. B. Ramos ◽  
Catarina Moreirinha ◽  
Sara Silva ◽  
Eduardo M. Costa ◽  
Mariana Veiga ◽  
...  
Keyword(s):  
High Performance ◽  
Radical Scavenging Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Phenolic Components ◽  
Polar Extracts ◽  
Salix Spp

Salix spp. have been exploited for energy generation, along with folk medicine use of bark extracts for antipyretic and analgesic benefits. Bark phenolic components, rather than salicin, have demonstrated interesting bioactivities, which may ensure the sustainable bioprospection of Salix bark. Therefore, this study highlights the detailed phenolic characterization, as well as the in vitro antioxidant, anti-hypertensive, Staphylococcus aureus growth inhibitory effects, and biocompatibility of Salix atrocinerea Brot., Salix fragilis L., and Salix viminalis L. bark polar extracts. Fifteen phenolic compounds were characterized by ultra-high-performance liquid chromatography-ultraviolet detection-mass spectrometry analysis, from which two flavan-3-ols, an acetophenone, five flavanones, and a flavonol were detected, for the first time, as their bark components. Salix bark extracts demonstrated strong free radical scavenging activity (5.58–23.62 µg mL−1 IC50 range), effective inhibition on angiotensin-I converting enzyme (58–84%), and S. aureus bactericidal action at 1250–2500 µg mL−1 (6–8 log CFU mL−1 reduction range). All tested Salix bark extracts did not show cytotoxic potential against Caco-2 cells, as well as S. atrocinerea Brot. and S. fragilis L. extracts at 625 and 1250 µg mL−1 against HaCaT and L929 cells. These valuable findings can pave innovative and safer food, nutraceutical, and/or cosmetic applications of Salix bark phenolic-containing fractions.

scholarly journals Hepatoprotective Effects of acerola cherry extract powder against d-galactosamine-induced liver injury in rats and its bioactive compounds

2004 ◽  
Vol 22 (SI - Chem. Reactions in Foods V) ◽  
pp. S159-S162 ◽  
Author(s):  
I. Nagamine ◽  
M. Fujita ◽  
I. Hongo ◽  
H. T T Nguyen ◽  
M. Miyahara ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Serum Levels ◽  
Lemon Juice ◽  
Protective Effects ◽  
Trimethylsilyl Ethers ◽  
Hepatic Lipid Peroxidation ◽  
Dpph Radical Scavenging

Treatment with the water and tropical lemon juice extract powders from acerola fruit purees and leaves (100 mg/kg) significantly ameliorates the hepatic inflammatory responses such as increased serum levels of AST, ALT, and GGT in rats subjected to acute D-galactosamine (GalN) intoxication. The protective effects of their constituents could be related to their antioxidant activities to neutralise free radicals to attenuate hepatic lipid peroxidation and thus can protect liver damage. The effect of the water extract powder from fruit purees (100 mg/kg) was moderately stronger than that of ascorbic acid (10 mg/kg), but weaker than that of cyanidin-3-O-rhamnoglucoside (13.3 mg/kg). The water and lemon juice extract powders from Acerola fruit purees possess the 18.6 and 24.1-fold higher DPPH radical scavenging activities, respectively, than those from leaves, the higher so for those extracted with lemon juice than for those extracted with water. The vitamin C contents were much more higher in the extract powders from fruit purees compared with those from leaves. γ-Tocopherol predominated in the extract powders from fruit purees and α-tocopherol in those from leaves. Polyphenolic compounds were identified and analysed by GC/MS-SIM after acid hydrolysis, extraction and derivatisation to trimethylsilyl ethers.

scholarly journals Chemical characterization, fatty acid profile and antioxidant activity of Gustavia macarenensis fruit mesocarp and its oil from the Amazonian region of Ecuador as an unconventional source of vegetable oil

2019 ◽  
Vol 70 (2) ◽  
pp. 298
Author(s):  
J. J. Reyes Mera ◽  
R. Abreu-Naranjo ◽  
J. M. Alvarez-Suarez ◽  
D. Viafara
Keyword(s):  
Antioxidant Activity ◽  
Fatty Acid ◽  
Fatty Acid Profile ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Characterization ◽  
Total Phenolic ◽  
Linear Discriminant ◽  
Chromatography Analysis

To our knowledge, this study is the first to report on the nutritional characterization, bioactive compounds and antioxidant activity of Amazonian G. macarenensis fruit. The fatty acid profile was determined using a high performance liquid chromatography analysis. The total phenolic content and antioxidant activity were determined using Folin Ciocalteu’s method and by radical scavenging activity, respectively. Moreover, a cluster analysis was carried out in order to classify the G. macarenensis fruit oil according to its fatty acid profile. Seven Fisher linear discriminant functions were obtained from the discriminant analysis. These models allow one to classify new fruits on the basis of their fatty acid profile. A high value for total lipids was obtained (53.57%). Its main components were palmitic and oleic acid. The TPC value (156.49 ± 2.62 mg GAE/Kg of oil) obtained from the G. macarenensis fruit pulp oil is higher than what was reported for some olive oil and Brazilian mango oil varieties.

scholarly journals Comparison of the major chemical constituents and antioxidant effects in Amauroderma rugosum and Ganoderma lucidum

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Renkai Li ◽  
Jingjing Li ◽  
Timothy Man-Yau Cheung ◽  
Bryan Siu-Yin Ho ◽  
George Pak-Heng Leung
Keyword(s):  
Ganoderma Lucidum ◽  
Healthy Aging ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Water Extract ◽  
Edible Mushroom ◽  
Total Phenolic ◽  
Protective Effects ◽  
Major Chemical ◽  
Antioxidant Effects

Aging is a major risk factor for many diseases, including cardiovascular diseases, neurological disorders, cancer and diabetes mellitus. Oxidative stress plays a key role in the aging process. Amauroderma rugosum is an edible mushroom that has rarely been studied. The aims of this study were to compare the major chemical constituents and to investigate the antioxidant effects of Amauroderma lucidum and Ganoderma lucidum. The water extract of Amauroderma lucidum contained a higher amount of total phenolic compounds than that of Ganoderma lucidum. The total polysaccharide and triterpene content in water extracts of Amauroderma rugosum and Ganoderma lucidum did not significantly differ. The water extract of Amauroderma rugosum demonstrated free radical scavenging capacity and could reduce doxorubicin-induced damage in H9c2 cardiomyoblasts. Amauroderma rugosum has stronger antioxidant and cellular protective effects than Ganoderma lucidum. Amauroderma rugosum may be beneficial in healthy aging, and further study should be encouraged

scholarly journals Isolation of Strong Antioxidants from Paeonia Officinalis Roots and Leaves and Evaluation of Their Bioactivities

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 249 ◽  
Author(s):  
Lijana Dienaitė ◽  
Milda Pukalskienė ◽  
Audrius Pukalskas ◽  
Carolina V. Pereira ◽  
Ana A. Matias ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Hydroxyl Radical ◽  
Antioxidant Capacity ◽  
High Performance ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Water Extract ◽  
Dependent Manner ◽  
Leaf Extracts

Paeonia officinalis extracts from leaves and roots were tested for their antioxidant potential using in vitro chemical (Folin-Ciocalteu, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), oxygen radical absorbance capacity (ORAC), hydroxyl radical antioxidant capacity (HORAC), hydroxyl radical scavenging capacity HOSC)) and cellular antioxidant activity (CAA) assays. Leaf extracts were stronger antioxidants than root extracts, while methanol was a more effective solvent than water in chemical assays. However, the selected water extract of leaves was a stronger antioxidant in CAA than the methanol extract (0.106 vs. 0.046 µmol quercetin equivalents/mg). Twenty compounds were identified by ultra performance liquid chromatography-quadrupole-time-of-flight (UPLC-Q-TOF) mass spectrometer, while on-line screening of their antioxidant capacity by high performance liquid chromatography (HPLC) with a DPPH•-scavenging detector revealed that gallic acid derivatives are the major peony antioxidants. Root water and leaf methanol extracts inhibited α-amylase in a dose dependent manner. The IC50 value for the strongest inhibitor, the methanol extract of leaves, was 1.67 mg/mL. In addition, the cytotoxicity assessment of extracts using human Caco-2 cells demonstrated that none of them possessed cytotoxic effects.

scholarly journals Potential antioxidant effects of Narcissus tazetta phenolic compounds against cadmium chloride induced hepatotoxicity in Swiss albino mice

2021 ◽  
Author(s):  
Saoussen Ben-Abdallah ◽  
Mediha Sefi ◽  
Nejla Soudani ◽  
Amel Hamdi ◽  
Safa Bejaoui ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Cadmium Chloride ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Protective Effects ◽  
Gamma Glutamyl Transpeptidase ◽  
Narcissus Tazetta ◽  
Protein Thiols

Abstract Narcissus tazetta (Amaryllidaceae) is a medicinal plant, widely used for cut flowers and potted ornamental plant in Tunisia flora. The current study evaluated the phenolic composition and antioxidant properties of its flower extracts and investigated its potential protective activity against cadmium chloride (CdCl2)-induced hepatotoxicity in mice. Adult Swiss mice were exposed either to CdCl2 (0.16 mg/kg bw) or to Narcissus tazetta extract (100 or mg/kg bw) or to their combination. Cadmium chloride and Narcissus tazetta flower extracts were given by intraperitoneal injection and gavage respectively for eight consecutive days. Results indicated that ethanolic flower extracts contained high levels of total phenolic and flavonoid contents along with a strong total antioxidant and 2,2-Diphenyl-1-picrylhydrazyl free radical scavenging activities. High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) analysis identified eight phenolic compounds, including rutin, Kaempferol glycosides and chlorogenic acids. The extract also exhibited marked hepato-protective effects against CdCl2 toxicity by reducing hepatic levels of malondialdehyde, advanced oxidation protein products, hydrogen peroxide, metallothioneins and DNA degradation. Additionally, co-administration of Narcissus tazetta flower extracts lowered the plasma activities of transaminases, gamma glutamyl transpeptidase and lactate dehydrogenase, and increased hepatic levels of reduced glutathione, non-protein thiols, vitamin C, and catalase activity. The hepatoprotective effects of the extract were demonstrated by histopathological improvement of liver disorders. The current study provided ethnopharmacological application of Narcissus tazetta flower extracts against CdCl2 induced oxidative stress, suggesting its chemoprevention role of its phenolic compounds as a natural antioxidant.

scholarly journals Protective Effect of Dealcoholized Persimmonwine on H2O2 - Induced Oxidative Injury in H9c2 Cardiomyocytes

2013 ◽  
Vol 2 (4) ◽  
pp. 61
Author(s):  
Jin Taek Hwang ◽  
Chan Kyu Han ◽  
Sang Yoon Choi ◽  
Sung Soo Kim
Keyword(s):  
Oxidative Stress ◽  
Gallic Acid ◽  
High Performance ◽  
Cell Injury ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Protective Effects ◽  
H9c2 Cardiomyocytes ◽  
Dose Dependent

<p>In this study, we investigated the antioxidant capacity of persimmon wine (PW) and dealcoholized persimmon wine (DPW). Both PW and DPW showed radical scavenging activity in the DPPH (1-diphenyl-2-picrylhydrazyl) assay. We next analyzed the phenolic content and major compounds present in PW using high-performance liquid chromatography (HPLC). Phenolic compounds, including gallic acid, catechin, and epicatechin, were found in PW. Gallic acid was the most abundant phenolic compound (157.5 µg/ml) in PW. In addition, the protective effects of DPW and gallic acid against H<sub>2</sub>O<sub>2</sub>-induced cell injury in H9c2 cardiomyocytes were investigated. Pretreatment with DPW or gallic acid strongly inhibited H<sub>2</sub>O<sub>2</sub>-induced cell death in a dose-dependent manner. These results suggested that PW and its major phenolic component, gallic acid, were effective inhibitors of oxidative stress and oxidative stress-induced cardiomyocyte injury.</p>

Sign in / Sign up

Export Citation Format

Share Document