scholarly journals Bactericidal and In Vitro Cytotoxicity of Moringa oleifera Seed Extract and Its Elemental Analysis Using Laser-Induced Breakdown Spectroscopy

2020 ◽  
Vol 13 (8) ◽  
pp. 193
Author(s):  
Reem K. Aldakheel ◽  
Suriya Rehman ◽  
Munirah A. Almessiere ◽  
Firdos A. Khan ◽  
Mohammed A. Gondal ◽  
...  
Keyword(s):  
Mtt Assay ◽  
Inhibitory Action ◽  
Moringa Oleifera ◽  
In Vitro Cytotoxicity ◽  
Laser Induced Breakdown Spectroscopy ◽  
Breakdown Spectroscopy ◽  
Laser Induced Breakdown ◽  
Hct 116 ◽  
Moringa Oleifera Seed

In the current study, we present the correlation between the capability of laser-induced breakdown spectroscopy (LIBS) to monitor the elemental compositions of plants and their biological effects. The selected plant, Moringa oleifera, is known to harbor various minerals and vitamins useful for human health and is a potential source for pharmaceutical interventions. From this standpoint, we assessed the antibacterial and in vitro cytotoxicity of the bioactive components present in Moringa oleifera seed (MOS) extract. Detailed elemental analyses of pellets of MOSs were performed via LIBS. Furthermore, the LIBS outcome was validated using gas chromatography–mass spectrometry (GC-MS). The LIBS signal was recorded, and the presence of the essential elements (Na, Ca, Se, K, Mg, Zn, P, S, Fe and Mn) in the MOSs were examined. The bactericidal efficacy of the alcoholic MOS extract was examined against Escherichia coli (E. coli) and Staphylococcus aureus(S. aureus) by agar well diffusion (AWD) assays and scanning electron microscopy (SEM), which depicted greater inhibition against Gram-positive bacteria. The validity and DNA nuclear morphology of human colorectal carcinoma cells (HCT-116) cells were evaluated via an MTT assay and DAPI staining. The MTT assay results manifested a profoundly inhibitory action of MOS extract on HCT116 cell growth. Additionally, MOS extracts produced inhibitory action in colon cancer cells (HCT-116), whereas no inhibitory action was seen using the same concentrations of MOS extract on HEK-293 cells (non-cancerous cells), suggesting that MOS extracts could be non-cytotoxic to normal cells. The antibacterial and anticancer potency of these MOS extracts could be due to the presence of various bioactive chemical complexes, such as ethyl ester and D-allose and hexadecenoic, oleic and palmitic acids, making them an ideal candidate for pharmaceutical research and applications.

scholarly journals Elemental analysis of Moringa oleifera seeds by Laser Induced Breakdown Spectroscopy (LIBS) and its anti-cancer and anti-microbial studies

2020 ◽  
Author(s):  
Mohammed A Gondal
Keyword(s):  
Cell Viability ◽  
Elemental Analysis ◽  
Moringa Oleifera ◽  
Laser Induced Breakdown Spectroscopy ◽  
Bioactive Components ◽  
Hek 293 ◽  
Breakdown Spectroscopy ◽  
Laser Induced Breakdown ◽  
Hct 116 ◽  
Microbial Potential

Moringa oleifera plant contains a numerous antioxidants, antibiotics and nutrients (vitamins and minerals) which makes it prospective for diverse biomedical applications. This report investigated the anti-cancerous and anti-microbial potential of the Moringa oleifera seeds (MOSs) due to the bioactive components a detail elemental analysis. MOSs in the form of pellets were used in elemental analysis. Alcoholic extraction of were utilized in anti-cancerous and anti-microbial activity.To clarify the anti-cancerous and anti-microbial potential of the (MOSs) because of the bioactive components. An elemental analysis was conducted using Laser Induced Breakdown Spectroscopy (LIBS) . The GC-MS used for the validation of the LIBS outcome. Additionally, the anti-cancerous and anti-microbial activity of the MOSs was evaluated. Herein, the human colorectal carcinoma cells (HCT-116) were treated with the seeds aqueous extracts for 48 h and the cell viability plus the DNA nuclear morphology were measured via the MTT assay and DAPI staining. The cell viability of the normal human embryonic stem cells (HEK-293) was also examined after the treatment. Percentage of cell viability and inhibitory concentration (IC 50 ) of both normal and treated cells were determined.The recorded LIBS signal of the MOSs revealed the existence of elements like Ca, K, Mg, P, S, Fe, Mn, Zn, Na and Se (vital for human health). The results of the MTT assay revealed a profound inhibitory action of the MOSs extracts against the HCT-116 cells growth. On top, such extracts did not affect the HEK-293 cells growth, indicating the specificity of the proposed extracts towards cancer cells escalation hindrance. The anti-microbial activity of the ethanolic MOSs extracts was tested on the S. aureus and E. coli bacteria using the Agar well diffusion assay. The observed anti-cancerous and the anti-microbial activities of the MOSs extracts can be attributed to the charisma of various bioactive compounds including the oleic acid, palmitic acid, hexadecenoic acid ethyl ester and D-allose. Conclusion: Current observations may contribute towards the development of the MOSs-based biomedicine (organic without any side effects, cheap, plentiful, pure and sustainable) effective for the cancer and bacterial infection cure.

scholarly journals Feasibility Study on Discrimination of Neo-plastic and Non-Neoplastic Gastric Tissues Using Spark Discharge Assisted Laser Induced Breakdown Spectroscopy

2018 ◽  
Vol 10 (1) ◽  
pp. 64-69 ◽  
Author(s):  
Aida Seifalinezhad ◽  
Maryam Bahreini ◽  
Mohammad Mahdi Hassani Matin ◽  
Seyed Hassan Tavassoli
Keyword(s):  
Light Emission ◽  
In Vitro Study ◽  
Cost Effective ◽  
Laser Pulses ◽  
Spark Discharge ◽  
Plasma Radiation ◽  
Laser Induced Breakdown Spectroscopy ◽  
Breakdown Spectroscopy ◽  
Laser Induced Breakdown

Introduction: The present work is a novel in vitro study that evaluated the possibility of diagnosing neoplastic from nonneoplastic gastric tissues using spark discharge assisted laser induced breakdown spectroscopy (SD-LIBS) method. Methods: In these experiments, the low energy laser pulses ablated a tiny amount of tissue surface leading to plasma formation. Then, a spark discharge was applied to plasma in order to intensify the plasma radiation. Light emission from plasma was recorded as spectra which were analyzed. Gastric tissues of 5 people were studied through this method. Results: The SD-LIBS technique had the potential to discriminate normal and cancerous tissues based on the significant differences in the intensities of some particular elements. The comparison of normalized calcium (Ca) and magnesium (Mg) peaks of neoplastic and nonneoplastic gastric tissues could be viewed as a practical measure for tissue discrimination since Ca and Mg peaks in spectra of neoplastic were noticeably higher than nonneoplastic. Conclusion: Considering the identification of gastric cancer, the applied method in these experiments seems quite fast, noninvasive and cost-effective with respect to other conventional methods. The significant increment of specific Ca and Mg lines of neoplastic gastric tissues in comparison to the nonneoplastic ones can be considered as valuable information that might bring about tissue classification. The number of samples in this work, however, was not sufficient for a decisive conclusion and further researches is needed to generalize this idea.

Nanosecond laser-induced breakdown assisted by femtosecond laser pre-ionization in air: the effect on spatial resolution and continuous radiation

2020 ◽  
Vol 92 (2) ◽  
pp. 20701
Author(s):  
Bo Li ◽  
Xiaofeng Li ◽  
Zhifeng Zhu ◽  
Qiang Gao
Keyword(s):  
Femtosecond Laser ◽  
Spatial Resolution ◽  
Laser Induced Breakdown Spectroscopy ◽  
Nanosecond Laser ◽  
Breakdown Threshold ◽  
Powerful Technique ◽  
Breakdown Spectroscopy ◽  
Continuous Radiation ◽  
Laser Induced Breakdown

Laser-induced breakdown spectroscopy (LIBS) is a powerful technique for quantitative diagnostics of gases. The spatial resolution of LIBS, however, is limited by the volume of plasma. Here femtosecond-nanosecond dual-pulsed LIBS was demonstrated. Using this method, the breakdown threshold was reduced by 80%, and decay of continuous radiation was shortened. In addition, the volume of the plasma was shrunk by 85% and hence, the spatial resolution of LIBS was significantly improved.

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