scholarly journals Genetic Analysis of Zoonotic Gastrointestinal Protozoa and Microsporidia in Shelter Cats in South Korea

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 894
Author(s):  
Dongmi Kwak ◽  
Min-Goo Seo
Keyword(s):  
Public Health ◽  
South Korea ◽  
Control Strategy ◽  
Parasitic Infection ◽  
Giardia Duodenalis ◽  
Enterocytozoon Bieneusi ◽  
Infection Rates ◽  
Intestinal Protozoa ◽  
Cryptosporidium Spp ◽  
Blastocystis Sp

Feral cats that are roaming outside can serve as reservoirs for zoonotic pathogens, negatively impacting public health. They may experience high levels of parasitic infection. Some gastrointestinal protozoa and microsporidia possessing zoonotic potential in cats include Cryptosporidium spp., Giardia duodenalis, Blastocystis sp., Enterocytozoon bieneusi, and Toxoplasma gondii. Here, we show the infection rates and risk factors of intestinal protozoa and microsporidia detected from shelter cats on Jeju Island in South Korea in 2020. Among 158 cats, we detected genes for five internal protozoa and microsporidia, namely, Cryptosporidium felis (0.6%), G. duodenalis (3.8%), Blastocystis sp. (0.6%), E. bieneusi (3.8%), and T. gondii (1.3%). Furthermore, 16 cats (10.1%) were PCR-positive for at least one protozoan or microsporidium. To our knowledge, this study is the first to describe the existence of C. felis, G. duodenalis assemblage F, Blastocystis sp. ST4 subtype, and E. bieneusi genotype Peru11 in cats in South Korea. Despite the small number of positive samples, this study expands our understanding of the incidence of zoonotic gastrointestinal protozoa and microsporidia in shelter cats and genetically characterizes the isolates found in the infected animals. Moreover, these findings emphasize the need for a better control strategy on protozoa and microsporidia in cats, tailored to their individual needs, to protect animal and public health.

scholarly journals Molecular Diversity of Giardia duodenalis, Cryptosporidium spp. and Blastocystis sp. in Asymptomatic School Children in Leganés, Madrid (Spain)

2020 ◽  
Vol 8 (4) ◽  
pp. 466 ◽  
Author(s):  
Aly Salimo Muadica ◽  
Pamela Carolina Köster ◽  
Alejandro Dashti ◽  
Begoña Bailo ◽  
Marta Hernández-de-Mingo ◽  
...  
Keyword(s):  
Giardia Duodenalis ◽  
Diarrhoeal Disease ◽  
Enterocytozoon Bieneusi ◽  
Health Concern ◽  
Personal Hygiene ◽  
Clinical Samples ◽  
Healthy Children ◽  
Pathogenic Potential ◽  
Cryptosporidium Spp ◽  
Blastocystis Sp

Enteric parasites including Giardia duodenalis, Cryptosporidium spp., and to a lesser extent, Blastocystis sp. and Enterocytozoon bieneusi, are major worldwide contributors to diarrhoeal disease. Assessing their molecular frequency and diversity is important to ascertain the sources of infection, transmission dynamics, and zoonotic potential. Little molecular information is available on the genotypes of these pathogens circulating in apparently healthy children. Here, we show that asymptomatic carriage of G. duodenalis (17.4%, 95% CI: 15.5–19.4%), Blastocystis sp. (13.0%, 95% CI: 11.4–14.8%), and Cryptosporidium spp. (0.9%, 95% CI: 0.5–1.5%) is common in children (1–16 years; n = 1512) from Madrid, Spain. Our genotyping data indicate that; (i) the observed frequency and diversity of parasite genetic variants are very similar to those previously identified in Spanish clinical samples, so that the genotype alone does not predict the clinical outcome of the infection, (ii) anthroponotic transmission accounts for a large proportion of the detected cases, highlighting that good personal hygiene practices are important to minimizing the risk of infection, (iii) Blastocystis ST4 may represent a subtype of the parasite with higher pathogenic potential, and (iv) Enterocytozoon bieneusi does not represent a public health concern in healthy children.

Infection rate of Giardia duodenalis , Cryptosporidium spp. and Enterocytozoon bieneusi in cashmere, dairy and meat goats in China

2016 ◽  
Vol 41 ◽  
pp. 26-31 ◽  
Author(s):  
Xian-Qi Peng ◽  
Ge-Ru Tian ◽  
Guan-Jing Ren ◽  
Zheng-Qing Yu ◽  
James Barron Lok ◽  
...  
Keyword(s):  
Infection Rate ◽  
Giardia Duodenalis ◽  
Enterocytozoon Bieneusi ◽  
Cryptosporidium Spp ◽  
Meat Goats

scholarly journals Symptomatic and Asymptomatic Protist Infections in Hospital Inpatients in Southwestern China

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 684
Author(s):  
Shun-Xian Zhang ◽  
David Carmena ◽  
Cristina Ballesteros ◽  
Chun-Li Yang ◽  
Jia-Xu Chen ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Giardia Duodenalis ◽  
Female Ratio ◽  
Cryptosporidium Spp ◽  
Male Female Ratio ◽  
Blastocystis Sp ◽  
Stool Samples ◽  
Etiological Agents ◽  
Age Range ◽  
Protist Species

Cryptosporidium spp., Entamoeba histolytica, Giardia duodenalis, and Blastocystis sp. infections have been frequently reported as etiological agents for gastroenteritis, but also as common gut inhabitants in apparently healthy individuals. Between July 2016 and March 2017, stool samples (n = 507) were collected from randomly selected individuals (male/female ratio: 1.1, age range: 38‒63 years) from two sentinel hospitals in Tengchong City Yunnan Province, China. Molecular (PCR and Sanger sequencing) methods were used to detect and genotype the investigated protist species. Carriage/infection rates were: Blastocystis sp. 9.5% (95% CI: 7.1–12.4%), G. duodenalis 2.2% (95% CI: 1.1–3.8%); and E. histolytica 2.0% (95% CI: 0.9–3.6%). Cryptosporidium spp. was not detected at all. Overall, 12.4% (95% CI: 9.7–15.6) of the participants harbored at least one enteric protist species. The most common coinfection was E. histolytica and Blastocystis sp. (1.0%; 95% CI: 0.3–2.2). Sequence analyses revealed that 90.9% (10/11) of the genotyped G. duodenalis isolates corresponded to the sub-assemblage AI. The remaining sequence (9.1%, 1/11) was identified as sub-assemblage BIV. Five different Blastocystis subtypes, including ST3 (43.7%, 21/48), ST1 (27.1%, 13/48), ST7 (18.8%, 9/48), ST4 (8.3%, 4/48), and ST2 (2.1%, 1/48) were identified. Statistical analyses confirmed that (i) the co-occurrence of protist infections was purely random, (ii) no associations were observed among the four protist species found, and (iii) neither their presence, individually or jointly, nor the patient’s age was predictors for developing clinical symptoms associated with these infections. Overall, these protist mono- or coinfections are asymptomatic and do not follow any pattern.

Molecular detection of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in school children at the Thai-Myanmar border

2021 ◽  
Author(s):  
Chantira Sutthikornchai ◽  
Supaluk Popruk ◽  
Aongart Mahittikorn ◽  
Dumrongkiet Arthan ◽  
Ngamphol Soonthornworasiri ◽  
...  
Keyword(s):  
School Children ◽  
Molecular Detection ◽  
Giardia Duodenalis ◽  
Enterocytozoon Bieneusi ◽  
Cryptosporidium Spp

scholarly journals Molecular Diversity of Giardia duodenalis, Cryptosporidium spp., and Blastocystis sp. in Symptomatic and Asymptomatic Schoolchildren in Zambézia Province (Mozambique)

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 255 ◽  
Author(s):  
Aly S. Muadica ◽  
Pamela C. Köster ◽  
Alejandro Dashti ◽  
Begoña Bailo ◽  
Marta Hernández-de-Mingo ◽  
...  
Keyword(s):  
Low Income ◽  
Molecular Diversity ◽  
Giardia Duodenalis ◽  
Molecular Data ◽  
Low Income Countries ◽  
Medical Attention ◽  
Cryptosporidium Spp ◽  
Asymptomatic Children ◽  
Common Causes ◽  
Blastocystis Sp

Infections by the protist enteroparasites Giardia duodenalis, Cryptosporidium spp., and, to a much lesser extent, Blastocystis sp. are common causes of childhood diarrhoea in low-income countries. This molecular epidemiological study assesses the frequency and molecular diversity of these pathogens in faecal samples from asymptomatic schoolchildren (n = 807) and symptomatic children seeking medical attention (n = 286) in Zambézia province, Mozambique. Detection and molecular characterisation of pathogens was conducted by polymerase chain reaction (PCR)-based methods coupled with Sanger sequencing. Giardia duodenalis was the most prevalent enteric parasite found [41.7%, 95% confidence interval (CI): 38.8–44.7%], followed by Blastocystis sp. (14.1%, 95% CI: 12.1–16.3%), and Cryptosporidium spp. (1.6%, 95% CI: 0.9–2.5%). Sequence analyses revealed the presence of assemblages A (7.0%, 3/43) and B (88.4%, 38/43) within G. duodenalis-positive children. Four Cryptosporidium species were detected, including C. hominis (30.8%; 4/13), C. parvum (30.8%, 4/13), C. felis (30.8%, 4/13), and C. viatorum (7.6%, 1/13). Four Blastocystis subtypes were also identified including ST1 (22.7%; 35/154), ST2 (22.7%; 35/154), ST3 (45.5%; 70/154), and ST4 (9.1%; 14/154). Most of the genotyped samples were from asymptomatic children. This is the first report of C. viatorum and Blastocystis ST4 in Mozambique. Molecular data indicate that anthropic and zoonotic transmission (the latter at an unknown rate) are important spread pathways of diarrhoea-causing pathogens in Mozambique.

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