scholarly journals Symptomatic and Asymptomatic Protist Infections in Hospital Inpatients in Southwestern China

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 684
Author(s):  
Shun-Xian Zhang ◽  
David Carmena ◽  
Cristina Ballesteros ◽  
Chun-Li Yang ◽  
Jia-Xu Chen ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Giardia Duodenalis ◽  
Female Ratio ◽  
Cryptosporidium Spp ◽  
Male Female Ratio ◽  
Blastocystis Sp ◽  
Stool Samples ◽  
Etiological Agents ◽  
Age Range ◽  
Protist Species

Cryptosporidium spp., Entamoeba histolytica, Giardia duodenalis, and Blastocystis sp. infections have been frequently reported as etiological agents for gastroenteritis, but also as common gut inhabitants in apparently healthy individuals. Between July 2016 and March 2017, stool samples (n = 507) were collected from randomly selected individuals (male/female ratio: 1.1, age range: 38‒63 years) from two sentinel hospitals in Tengchong City Yunnan Province, China. Molecular (PCR and Sanger sequencing) methods were used to detect and genotype the investigated protist species. Carriage/infection rates were: Blastocystis sp. 9.5% (95% CI: 7.1–12.4%), G. duodenalis 2.2% (95% CI: 1.1–3.8%); and E. histolytica 2.0% (95% CI: 0.9–3.6%). Cryptosporidium spp. was not detected at all. Overall, 12.4% (95% CI: 9.7–15.6) of the participants harbored at least one enteric protist species. The most common coinfection was E. histolytica and Blastocystis sp. (1.0%; 95% CI: 0.3–2.2). Sequence analyses revealed that 90.9% (10/11) of the genotyped G. duodenalis isolates corresponded to the sub-assemblage AI. The remaining sequence (9.1%, 1/11) was identified as sub-assemblage BIV. Five different Blastocystis subtypes, including ST3 (43.7%, 21/48), ST1 (27.1%, 13/48), ST7 (18.8%, 9/48), ST4 (8.3%, 4/48), and ST2 (2.1%, 1/48) were identified. Statistical analyses confirmed that (i) the co-occurrence of protist infections was purely random, (ii) no associations were observed among the four protist species found, and (iii) neither their presence, individually or jointly, nor the patient’s age was predictors for developing clinical symptoms associated with these infections. Overall, these protist mono- or coinfections are asymptomatic and do not follow any pattern.

scholarly journals Molecular Detection and Genotyping of Enteric Protists in Asymptomatic Schoolchildren and Their Legal Guardians in Madrid, Spain

Parasitologia ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 83-94
Author(s):  
Pamela Carolina Köster ◽  
Alejandro Dashti ◽  
Lucia Reh ◽  
Begoña Bailo ◽  
Marta Hernández-de-Mingo ◽  
...  
Keyword(s):  
Giardia Duodenalis ◽  
Epidemiological Survey ◽  
Healthy Children ◽  
Cryptosporidium Spp ◽  
Legal Guardians ◽  
Household Transmission ◽  
Blastocystis Sp ◽  
Stool Samples ◽  
Single Child ◽  
Assemblage B

Asymptomatic carriage of diarrhoea-causing enteric protist parasites in the general population is poorly understood, particularly in medium- to high-income countries. This molecular epidemiological survey investigates the presence, molecular diversity, and household transmission of Giardia duodenalis, Cryptosporidium spp., Blastocystis sp., and Enterocystozoon bieneusi in schoolchildren aged 2–13 years (n = 74) and their legal guardians (n = 6) in Madrid, Spain. Enteroparasite detection and genotyping was conducted in stool samples by molecular (PCR and Sanger sequencing) methods. Potential associations linked to infections were investigated through epidemiological questionnaires. Giardia duodenalis was the most prevalent enteric parasite found (14%, 95% CI: 7.1–23), followed by Blastocystis sp. (10%, 95% CI: 6.2–22) and Cryptosporidium spp. (3.8%, 95% CI: 0.78–11). None of the participants tested positive for E. bieneusi. Sequence analyses revealed the presence of G. duodenalis assemblage B, sub-assemblage BIV in a single child. The three Cryptosporidium isolates obtained were assigned to C. hominis, two of them belonging to the gp60 subtype IbA10G2. Four Blastocystis subtypes were identified including ST2 (38%, 3/8), ST3 (25%, 2/8), ST4 (25%, 2/8), and ST8 (12%, 1/8). All G. duodenalis and Cryptosporidium isolates were detected in children only. Blastocystis ST3 and ST4 were circulating in members of the same household. Blastocystis carriage rates increased with the age of the participants. Presence of diarrhoea-causing enteric protists was common in apparently healthy children.

scholarly journals Clinicopathological features of pilomatricoma cases: Analysis of 21 cases

2014 ◽  
Vol 4 (7) ◽  
pp. 530-533
Author(s):  
D Ghartimagar ◽  
A Ghosh ◽  
K Gautam ◽  
S Thapa ◽  
SR Shrestha ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Needle Aspiration ◽  
Common Finding ◽  
Clinical Impression ◽  
Aspiration Cytology ◽  
Female Ratio ◽  
Male Female Ratio ◽  
Needle Aspiration Cytology ◽  
Age Range ◽  
Slow Growing

Background: Pilomatricoma is a slow-growing, asymptomatic tumour originating from outer sheath cells of hair follicle. In this study, we describe the clinical presentation and histopathological features of pilomatricoma. Materials and Methods: This was a hospital based retrospective study carried out in all patients who were diagnosed as pilomatricoma over a period of January 2001 to December 2013. The study was done in department of pathology, Manipal Teaching Hospital, Nepal. Results: A total of 21 cases of pilomatricoma were reported with age range of 9-53 years (mean age 23.7) and male female ratio of 1:1.1. The most common site of occurrence was neck and preauricular region. The size of the tumour ranged from 0.3 to 4.7cm with a mean of 1.2cm. Multiple occurrences were seen in 3 patients and ossifying pilomarticoma was seen in 4 cases. Conclusion: Pilomatricoma is a benign skin neoplasm of hair follicle matrix cells. Calcification is a common finding while dystrophic ossification also can occur in the tumour. Histopathology gives the definite diagnosis as fine needle aspiration cytology and clinical impression may be misleading. DOI: http://dx.doi.org/10.3126/jpn.v4i7.10293 Journal of Pathology of Nepal (2014) Vol. 4, 530-533

Highly sensitive and specific detection of Giardia duodenalis, Entamoeba histolytica, and Cryptosporidium spp. in human stool samples by the BD MAX™ Enteric Parasite Panel

2017 ◽  
Vol 117 (2) ◽  
pp. 447-451 ◽  
Author(s):  
Marijo Parčina ◽  
Ingrid Reiter-Owona ◽  
Frank P. Mockenhaupt ◽  
Valerija Vojvoda ◽  
Jean Bosco Gahutu ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Giardia Duodenalis ◽  
Specific Detection ◽  
Cryptosporidium Spp ◽  
Highly Sensitive ◽  
Stool Samples ◽  
Human Stool

scholarly journals Multiple Fatal Intoxications Caused by Improper Consumption of an Alcoholic Para-Pharmaceutical Product

2019 ◽  
Vol 70 (7) ◽  
pp. 2471-2476
Author(s):  
Camelia Liana Buhas ◽  
Bogdan Adrian Buhas ◽  
Lucia Georgeta Daina ◽  
Bianca Hanganu ◽  
Irina Smaranda Manoilescu ◽  
...  
Keyword(s):  
Pharmaceutical Products ◽  
Pharmaceutical Product ◽  
Chronic Ethanol ◽  
Deep Coma ◽  
Female Ratio ◽  
Examination Result ◽  
Chronic Ethanol Consumption ◽  
Male Female Ratio ◽  
Severe Intoxication ◽  
Age Range

Ingestion of cheap para-pharmaceuticals (such as disinfectants, deodorizing solutions, etc.) which are designed only for external use is an unfortunate habit encountered in the recent decades in the economically disadvantaged areas of Romania inhabited by poor, uninformed, and sanitary uneducated population. These para-pharmaceutical products are based on different concentrations of ethanol. Occasionally, the manufacturer modifies the product formula, or worse omits on the label that the ethanol was replaced with methanol, resulting in mass poisoning with a large number of casualties. The authors present a case of mass poisoning by methanol that occurred during one month and resulted in 40 cases of methanol intoxication. Only 5 out of the 40 victims survived the intoxication. All the dead victims underwent medico-legal autopsy which revealed only general features, liable to poisoning. The toxicological examination result was positive for methanol in all the cases. All the intoxicated victims were homeless; they were heavy ethanol consumers, especially of the product rubbing alcohol. The male: female ratio was 31:4 and the age range was between 25 and 70 years old, with an average of 50 years. All the victims were hospitalized in deep coma, showing obvious pathological changes specific to chronic ethanol consumption. Conclusions: some alcoholic para-pharmaceutical products manufactured for external use are ingested by chronic ethanol drinkers. When these products contain methanol, they can cause severe intoxication followed by an impressively large number of deaths.

scholarly journals Early Outcome of Laparoscopic Abdominoperineal Resection, Our Experience in Bangabandhu Sheikh Mujib Medical University

2014 ◽  
Vol 8 (2) ◽  
pp. 53-55
Author(s):  
Mohammed Tanvir Jalal ◽  
Shahadot Hossain Sheikh ◽  
Md Rayhanur Rahman ◽  
Mesbah Uddin Noman ◽  
Jesmin Akhter Leena ◽  
...  
Keyword(s):  
Abdominoperineal Resection ◽  
Operative Procedure ◽  
Operation Time ◽  
Postoperative Outcomes ◽  
Female Ratio ◽  
Early Outcome ◽  
Male Female Ratio ◽  
Laparoscopic Abdominoperineal Resection ◽  
Medical University ◽  
Age Range

Anorectal malignancies that require abdominoperineal resection (APR) is very common. Laparoscopic APR can be a better option. Laparoscopic APR has been seldom studied. This study aims to evaluate perioperative and early postoperative outcomes of laparoscopic APRs performed for the treatment of ano-rectal carcinomas. Patients operated for ano-rectal carcinoma between June 2011 to June 2013 in Bangabandhu Sheikh Mujib Medical University (BSMMU) were observed. Demographics, tumor and procedure-related parameters, perioperative results, early postoperative outcomes and survival were observed. Total 22 patients were under went laparoscopic APR. Male: Female ratio was 15:7 (68.18%: 31.82%). Age range was from 30-65 years with a mean age of 36.55 years. Mean operation time was 165 minutes and mean post-operative hospital stay was 6.8 days. Overall complication rate was 45.45%. Laparoscopic APR is a safe, effective and technically feasible procedure. It can be a better operative procedure than open APR. DOI: http://dx.doi.org/10.3329/fmcj.v8i2.20281 Faridpur Med. Coll. J. 2013;8(2): 53-55

scholarly journals Evaluation of intestinal parasites in patients with chronic spontaneous urticaria in a territory hospital in Turkey

2019 ◽  
Vol 13 (10) ◽  
pp. 927-932
Author(s):  
Sedat Vezir ◽  
Filiz Kaya ◽  
Emine Vezir ◽  
Nermin Karaosmanoğlu ◽  
Ali Kudret Adiloğlu
Keyword(s):  
Patient Group ◽  
Chronic Urticaria ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Adult Patients ◽  
Chronic Spontaneous Urticaria ◽  
Parasitic Infections ◽  
Cutaneous Manifestations ◽  
Blastocystis Sp ◽  
Stool Samples

Introduction: Chronic spontaneous urticaria (CSU) which develops without a known stimulation is defined as the occurrence of spontaneous wheals, angioedema or both for longer than six weeks. Infections, autoimmunity, food intolerance and internal parasitic infections are supposed to be underlying causes of CSU. The aim of this study was to evaluate the intestinal parasites in children and adult patients diagnosed as CSU, to determine the frequency of parasites in chronic urticaria, and to compare these patients with healthy demographic control groups. Methodology: Seventy six children and 38 adult patients with CSU were examined in terms of parasitic infections. The patients whom parasites were detected received anti-parasitic therapy and the improvements in CSU symptoms were evaluated. Stool samples were examined with direct microscopic examination (native-lugol), stool concentration and trichrome staining methods. Results: In pediatric patient group, 18.4% (n = 14) of the stool samples were positive for Blastocystis sp., 2.6% (n = 2), Dientamoeba fragilis and 1.3% (n = 1), Giardia duodenalis. In adult patient group, Blastocystis sp. was detected in 18.4% (n = 7) of the stool samples. Anti-parasitic therapy yielded substantial improvement in urticaria symptoms in 57.1% of pediatric and 60.0% of adult patients. Conclusions: Blastocystis sp. and D. fragilis may play a role in chronic urticaria which seriously disrupts the patient's quality of life. Parasitic infections should not be neglected in patients with cutaneous manifestations.

scholarly journals Cognitive Style of Humanities, Commerce and Science Students – A Study on Higher Secondary Students from West Bengal

2016 ◽  
Vol 3 (2) ◽  
Author(s):  
Sukrita Mukherjee ◽  
Dr. Ishita Chatterjee
Keyword(s):  
Cognitive Style ◽  
Secondary Students ◽  
Economic Status ◽  
Science Students ◽  
School Students ◽  
Female Ratio ◽  
Male Female Ratio ◽  
Educational Guidance ◽  
And Gender ◽  
Age Range

Cognitive style or thinking style is a term used in cognitive psychology to describe the way individuals think, perceive and remember information. The present study aims to understand the cognitive style adapted by higher secondary students. The study sample consists of 90 higher secondary school students (30 each from science, humanities and commerce background). The male female ratio was 50:50. The age range was from 16-18 years. They were all from middle socio-economic status. They were administered an adapted version of cognitive style questionnaire by Ancona, Kochan, Scully, Van maanen and Westney. The results indicate for the dimension of Sensing there was significant effect of stream of study and gender. For the Intuition dimension again there was a significant effect of stream and gender. For Perceiving dimension also there was a significant effect of stream and gender. Sensing was highest for the science stream and lowest for the Humanities. Again Intuition was highest for the Humanities stream and lowest for the Science stream. The males have always scored highest in the dimension of Perceiving than their female counterparts both in Humanities and Science stream. And the female irrespective of the stream of study has scored higher in the Intuitive dimension. The present study is helpful in the field of designing educational guidance and curriculum for the Higher Secondary Students.

scholarly journals Prevalence of Lymphoma Cancer in Punjab, Pakistan

2015 ◽  
Vol 3 (2) ◽  
pp. 342-346
Author(s):  
Muhammad Zohaib Nawaz ◽  
Muhammad Bilal ◽  
Muhammad Aamer Mehmood ◽  
Muhammad Asgher
Keyword(s):  
Health Program ◽  
Cancer Type ◽  
Future Health ◽  
Female Ratio ◽  
Low Socioeconomic ◽  
Leading Role ◽  
High Magnitude ◽  
Male Female Ratio ◽  
Age Range ◽  
Registry Record

Pakistan has a high magnitude of cancer from its independence. The burden of lymphoma cancer continues to increase in Pakistan. The low socioeconomic districts of Punjab such as those in Faisalabad most severely affected by lymphoma. The objective of this study was to analyze the lymphoma cancer registry record of patients from the districts in Faisalabad in order to achieve a net lymphoma incidence for use in raising future health program against cancer. The medical records of 110 patients registered at the Punjab Institute of Nuclear Medicine in Faisalabad were investigated. Information regarding age, gender, socioeconomic status, affected sites and cancer type were obtained. Among 110 cases of lymphoma cancer, the male: female ratio was 3:1, with an age range of 5-85 years. Of the 110 patients, 74% were male and 26% were female. Similar to the subtype distribution of lymphoma in the different areas of Faisalabad and its surroundings, Non-Hodgkin’s lymphoma (NHL) was more frequent, 74 cases (67%) than Hodgkin’s disease (HD), 36 cases (33%).The leading cancers were breast 36% (n=248), lymphoma 16% (n=110), and prostate cancer 10% (n=68). The growing burden of cancer especially lymphoma in Faisalabad is extremely alarming. Since the current work was an initial investigation, it could play a leading role in prevention and future planning regarding the battle against lymphoma in Pakistan.Int J Appl Sci Biotechnol, Vol 3(2): 342-346 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12756 

scholarly journals Genetic Analysis of Zoonotic Gastrointestinal Protozoa and Microsporidia in Shelter Cats in South Korea

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 894
Author(s):  
Dongmi Kwak ◽  
Min-Goo Seo
Keyword(s):  
Public Health ◽  
South Korea ◽  
Control Strategy ◽  
Parasitic Infection ◽  
Giardia Duodenalis ◽  
Enterocytozoon Bieneusi ◽  
Infection Rates ◽  
Intestinal Protozoa ◽  
Cryptosporidium Spp ◽  
Blastocystis Sp

Feral cats that are roaming outside can serve as reservoirs for zoonotic pathogens, negatively impacting public health. They may experience high levels of parasitic infection. Some gastrointestinal protozoa and microsporidia possessing zoonotic potential in cats include Cryptosporidium spp., Giardia duodenalis, Blastocystis sp., Enterocytozoon bieneusi, and Toxoplasma gondii. Here, we show the infection rates and risk factors of intestinal protozoa and microsporidia detected from shelter cats on Jeju Island in South Korea in 2020. Among 158 cats, we detected genes for five internal protozoa and microsporidia, namely, Cryptosporidium felis (0.6%), G. duodenalis (3.8%), Blastocystis sp. (0.6%), E. bieneusi (3.8%), and T. gondii (1.3%). Furthermore, 16 cats (10.1%) were PCR-positive for at least one protozoan or microsporidium. To our knowledge, this study is the first to describe the existence of C. felis, G. duodenalis assemblage F, Blastocystis sp. ST4 subtype, and E. bieneusi genotype Peru11 in cats in South Korea. Despite the small number of positive samples, this study expands our understanding of the incidence of zoonotic gastrointestinal protozoa and microsporidia in shelter cats and genetically characterizes the isolates found in the infected animals. Moreover, these findings emphasize the need for a better control strategy on protozoa and microsporidia in cats, tailored to their individual needs, to protect animal and public health.

Demographic Analysis Of 90 Polycythemia Vera Egyptian Cases :  An Egyptian Reference Center Experience

Blood ◽  
2013 ◽  
Vol 122 (21) ◽  
pp. 5245-5245
Author(s):  
Mervat Mattar ◽  
Nihad Tawfik ◽  
Noha Elhosseiny ◽  
Mohamed Morad
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Polycythemia Vera ◽  
Gastrointestinal Symptoms ◽  
Female Ratio ◽  
Reference Center ◽  
Vein Thrombosis ◽  
Male Female Ratio ◽  
Lost To Follow Up ◽  
Age Range

Abstract Objective We report 90 cases of Polycythemia Vera ( PV )during the period between 2003 and 2013 followed up in an adult Cairo  hematology reference center Results Among 90 PV cases registered between 2003 and 2013, sixty eight cases (75.6%) of  whompresented in the last three years alone.  The age range was between 19 and 72 years with a mean of 53.5 years and 25 cases ( 27.78%) presented in the age group between 50-55 years.   Male : female ratio was 1.3:1.Fifty per cent of patients were urban while 50% lived in the country. Symptoms included headache (24 cases) (26.67%), tinnitus (7 cases) (7.78%), fatigue (14 cases) (15.56%), weight loss (5 cases)(5.56%), fever and sweating( 7 cases)( 7.78%) , bone or joint pains (22 cases)(24.44%), parasthesias (13 cases)(14.44%), pruritis(7 cases) (7.78%)  or abdominal discomfort (16 cases)(17.78% (Fig 1) Figure (1) : Incidence Of Symptoms Among 90 Egyptian PV Cases Severity of symptoms was assessed by a simple score of zero (none), +1 (mild),+ 2 (moderate) and +3 (severe) (table1) Nine cases (10%) presented by venous thrombosis , 4 of whom had portal vein thrombosis (4.44%) Cerebro-vascular strokes were noted in 5 cases (5.56%) and acute myocardial infarction in 2 cases (2.22%). Splenomegaly was noted in 29 cases (32.22%) of cases and hepatomegaly in 13 cases (14.44%). Associated conditions included hypertension in 24 cases (26.67%), gastrointestinal symptoms in 16 cases(17.78%) Diabetes mellitus in 15 cases (16.67%),Hepatitis C virus infection in 6 cases ( 6.67%), Hepatitis B in 2 cases ( 2.26%), coronory heart disease in 5 cases (5 .56%) and renal problems in 3 cases ( 3.33%). Bleeding episodes included 6 (6.67%) GI and 3(3.33%) gum bleeds. Familial MPN, Cancer prostate, intestinal Schistosomiasis   and post-liver transplant done post- hepatitis C virus cirrhosis were reported in one case each. Twenty two cases(24.44%) were controlled on Hydroxyuria only,16 cases 17.78%) on venesection plus hydroxyuria,16 cases(17.78%) on venesection only. Progression  was noted in 13 cases (14.44%) with marrow fibrosis in five cases (5.56%) and 14 cases (15.56%) were lost to follow-up. Mortality was seen in 10% (nine cases). Conclusion PV among Egyptians may have distinct presentations regarding incidence, age and  presentation.Simpler methods of symptom severity assessments may be more suitable for Egyptian patients. Disclosures: Mattar: Novartis, BMS, Amgen: Honoraria.

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