scholarly journals Frequency of Detection and Phylogenetic Analysis of Porcine circovirus 3 (PCV-3) in Healthy Primiparous and Multiparous Sows and Their Mummified Fetuses and Stillborn

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 533 ◽  
Author(s):  
Viviane Saporiti ◽  
Susanna Martorell ◽  
Taís F. Cruz ◽  
Francini Klaumann ◽  
Florencia Correa-Fiz ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Porcine Circovirus ◽  
Reproductive Parameters ◽  
Reproductive Disorders ◽  
Time Points ◽  
Lack Of Information ◽  
Complete Genomes ◽  
Reproductive Disease ◽  
Porcine Circovirus 3 ◽  
Intrauterine Infections

Porcine circovirus 3 (PCV-3) has been suggested as a putative causal agent of swine reproductive disease. A number of different studies have pointed out this association, but there is still a lack of information regarding the normal rates of PCV-3 infection in farms with normal reproductive parameters. The objective of the present study was to assess the frequency of PCV-3 detection in primiparous and multiparous sows and in tissues from their respective fetuses from farms with average reproductive parameters. Sera from 57 primiparous and 64 multiparous sows from 3 different farms were collected at two time points. Brain and lung tissues from 49 mummies and 206 stillborn were collected at farrowing. Samples were tested by PCR, and when positive, quantified by quantitative PCR. Thirty-nine complete genomes were obtained and phylogenetically analyzed. All sera from multiparous sows were negative, while 19/57 (33.3%) primiparous sows were PCV-3 PCR positive. From the 255 tested fetuses, 86 (33.7%) had at least one tissue positive to PCV-3. The frequency of detection in fetuses from primiparous sows (73/91, 80.2%) was significantly higher than those from multiparous ones (13/164, 7.9%). It can be concluded that PCV-3 is able to cause intrauterine infections in absence of overt reproductive disorders.

scholarly journals First detection and phylogenetic analysis of porcine circovirus 3 in female donkeys with reproductive disorders

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Tongtong Wang ◽  
Wenqiong Chai ◽  
Yonghui Wang ◽  
Wenqiang Liu ◽  
Zetong Huang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Real Time Pcr ◽  
Large Scale ◽  
Clinical Signs ◽  
Reproductive Failure ◽  
Porcine Circovirus ◽  
Deer Mice ◽  
Reproductive Disorders ◽  
Blood Samples ◽  
Porcine Circovirus 3

Abstract Background PCV3 is a pathogen associated with porcine dermatitis and nephropathy syndrome (PDNS)-like clinical signs, reproductive failure, and cardiac and multiorgan inflammation, which was newly identified in 2016 in sows in USA. Recently, PCV3 has also been identified from several non-porcine species like (cattle, dog, wild boar, deer, mice and ticks). However, PCV3 infection in donkey is not well established. Since 2019, 300 blood samples were collected from female donkey, which was characterized by abortion and sterility, in Liaocheng city of China. Results In the present study, an investigation of PCV3 in donkey blood samples was undertaken employing by real time PCR. Positive rates of PCV3 in donkeys reach to 21.0 %. In addition, one full-length PCV3 genome sequence was obtained, and it had a highest identity with porcine circovirus 3 PCV3/CN/Nanjing2017 strain and is clustered to PCV3a genotype based on ORF2 sequences. Conclusions This is the first report of detection of PCV3 from female donkeys presenting reproductive failure in large-scale donkey farms, China. In addition, the PCV3 strain identified in this study shared the closest relationship with those from porcine, suggesting that PCV3 may be transmitted from pigs to donkeys. Totally, PCV3 infection in donkey should be concerned although the association between it and reproductive failure are not better understood.

Detection and phylogenetic analysis of porcine circovirus 3 in part of northern China from 2016 to 2018

2020 ◽  
Vol 165 (9) ◽  
pp. 2003-2011
Author(s):  
Wenyuan Gu ◽  
Qiankai Shi ◽  
Leyi Wang ◽  
Jianlou Zhang ◽  
Guangfu Yuan ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Northern China ◽  
Porcine Circovirus ◽  
Porcine Circovirus 3

scholarly journals Porcine Circovirus 3 Detection in Aborted Fetuses and Stillborn Piglets from Swine Reproductive Failure Cases

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 264
Author(s):  
Viviane Saporiti ◽  
Laura Valls ◽  
Jaime Maldonado ◽  
Mónica Perez ◽  
Florencia Correa-Fiz ◽  
...  
Keyword(s):  
High Viral Load ◽  
Reproductive Failure ◽  
Porcine Circovirus ◽  
Respiratory Syndrome Virus ◽  
Porcine Parvovirus ◽  
Reproductive Disease ◽  
Porcine Circovirus 3 ◽  
Clinical Conditions ◽  
Domestic Swine

Porcine circovirus 3 (PCV-3) has been widely detected in healthy and diseased pigs; among different pathologic conditions, the strongest evidence of association comes from reproductive disease cases. However, simple viral detection does not imply the causality of the clinical conditions. Detection of PCV-3 within lesions may provide stronger evidence of causality. Thus, this study aimed to assess the frequency of PCV-3 detection in tissues from fetuses/stillborn piglets in cases of reproductive problems in domestic swine, as well as the histopathologic assessment of fetal tissues. Fetuses or stillborn piglets from 53 cases of reproductive failure were collected and analyzed by PCV-3 qPCR. The presence of porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus 2 (PCV-2), and porcine parvovirus 1 (PPV1) was also checked. PCV-3 qPCR positive samples with a high viral load were tested by PCV-3 in situ hybridization (ISH), sequenced, and phylogenetically analyzed. PCV-3 DNA was detected in 18/53 (33.9%) reproductive failure cases and in 16 of them PCV-3 was the only pathogen found. PCV-2 DNA was found in 5/53 (9.4%), PRRSV RNA in 4/53 (7.5%) and PPV1 was not detected. Four out of the six PCV-3 qPCR-positive cases with Ct value <30 were positive when tested by ISH. In these samples, PCV-3 was detected within mild histopathologic lesions, such as arteritis and periarteritis in multiple tissues. The present work emphasizes the need to include PCV-3 as a potential causative agent of reproductive failure in swine.

scholarly journals Recent Progress on Epidemiology and Pathobiology of Porcine Circovirus 3

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1944
Author(s):  
Si Chen ◽  
Liying Zhang ◽  
Xue Li ◽  
Guyu Niu ◽  
Linzhu Ren
Keyword(s):  
Immune Responses ◽  
Respiratory Diseases ◽  
Porcine Circovirus ◽  
Multiple Infections ◽  
Affected Animal ◽  
Intermediate Hosts ◽  
Reproductive Disorders ◽  
Swine Industry ◽  
Digestive Disorders ◽  
Porcine Circovirus 3

The recently discovered porcine circovirus 3 (PCV3) belongs to the Circovirus genus of the Circoviridae family together with the other three PCVs, PCV1, PCV2, and PCV4. As reported, PCV3 can infect pig, wild boar, and several other intermediate hosts, resulting in single or multiple infections in the affected animal. The PCV3 infection can lead to respiratory diseases, digestive disorders, reproductive disorders, multisystemic inflammation, and immune responses. Up to now, PCV3 infection, as well as the disease caused by PCV3, has been reported in many swine farms worldwide with high positive rates, which indicates that the virus may be another important pathogen in the swine industry. Therefore, we reviewed the current progress on epidemiology and pathobiology of PCV3, which may provide the latest knowledge of the virus and PCV3-related diseases.

Molecular Characterization of Novel Porcine Circovirus 3 (PCV3) in Pig Populations in the North of Vietnam

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Nguyen Van Giap ◽  
Chung Hee Chun ◽  
Huynh Thi My Le ◽  
Cao Thi Bich Phuong ◽  
Vu Thi Ngoc ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Porcine Circovirus ◽  
The North ◽  
Porcine Circovirus 3

Enzymatic recombinase amplification coupled with CRISPR-Cas12a for ultrasensitive, rapid, and specific Porcine circovirus 3 detection

2021 ◽  
Vol 59 ◽  
pp. 101763
Author(s):  
Wuyin Zhang ◽  
Liang Xu ◽  
Qi Liu ◽  
Yingli Cao ◽  
Kankan Yang ◽  
...  
Keyword(s):  
Porcine Circovirus ◽  
Porcine Circovirus 3

scholarly journals Molecular detection and clinical aspects of porcine circovirus 3 infection in pigs from Brazil

2020 ◽  
Vol 72 (5) ◽  
pp. 1731-1736
Author(s):  
A.C.M. Cruz ◽  
I.L.F. Rodrigues ◽  
A.E. Souza ◽  
F.B. Knackfuss ◽  
R.L. Silveira ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Porcine Circovirus ◽  
Southeastern Brazil ◽  
Clinical Aspects ◽  
Statistical Association ◽  
Serum Samples ◽  
Clinical Presentations ◽  
Porcine Circovirus 3 ◽  
Clinical Conditions ◽  
Swine Serum

ABSTRACT Porcine circovirus 3 (PCV-3) DNA has been detected in serum samples from apparently healthy pigs as well as pigs with different clinical conditions. Molecular detection of PCV-3 was observed in swine serum samples from Southeastern - Brazil using a nested PCR designed specifically for this study. The epidemiology and clinical aspects of PCV-3 infection were evaluated. The samples originated from 154 pigs of both genders from different production phases and with different clinical presentations, sampled from 31 pig farms visited between 2013 and 2018. In this study, PCV-3 was detected in 26.7% of samples from all populations across varying ages. Statistical association (P=0.0285) was observed only between animals with respiratory signs and PCV-3; no PCV-3-positive animal had diarrhea. No statistical association was observed between PCV-3 and age, or gender of the pigs. Because PCV-3 is a newly discovered virus, there is very little information about its epidemiology. We hope that these data can help in future studies investigating PCV-3 epidemiology.

Retrospective survey and phylogenetic analysis of porcine circovirus type 3 in Jiangsu province, China, 2008 to 2017

2018 ◽  
Vol 163 (9) ◽  
pp. 2531-2538 ◽  
Author(s):  
Dong Zhao ◽  
Xiaobo Wang ◽  
Qingqing Gao ◽  
Changchao Huan ◽  
Wanbin Wang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Porcine Circovirus Type ◽  
Jiangsu Province ◽  
Porcine Circovirus ◽  
Retrospective Survey ◽  
Type 3
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