scholarly journals Genome Mining and Comparative Analysis of Streptococcus intermedius Causing Brain Abscess in a Child

Pathogens ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 22 ◽  
Author(s):  
Elio Issa ◽  
Tamara Salloum ◽  
Balig Panossian ◽  
David Ayoub ◽  
Edmond Abboud ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Metal Ions ◽  
Genome Evolution ◽  
Brain Abscess ◽  
Survival Rates ◽  
Genome Mining ◽  
Genomic Islands ◽  
Polymorphism Analysis ◽  
Streptococcus Intermedius ◽  
Pan Genome

Streptococcus intermedius (SI) is associated with prolonged hospitalization and low survival rates. The genetic mechanisms involved in brain abscess development and genome evolution in comparison to other members of the Streptococcus anginosus group are understudied. We performed a whole-genome comparative analysis of an SI isolate, LAU_SINT, associated with brain abscess following sinusitis with all SI genomes in addition to S. constellatus and S. anginosus. Selective pressure on virulence factors, phages, pan-genome evolution and single-nucleotide polymorphism analysis were assessed. The structural details of the type seven secretion system (T7SS) was elucidated and compared with different organisms. ily and nanA were both abundant and conserved. Nisin resistance determinants were found in 47% of the isolates. Pan-genome and SNPs-based analysis didn’t reveal significant geo-patterns. Our results showed that two SC isolates were misidentified as SI. We propose the presence of four T7SS modules (I–IV) located on various genomic islands. We detected a variety of factors linked to metal ions binding on the GIs carrying T7SS. This is the first detailed report characterizing the T7SS and its link to nisin resistance and metal ions binding in SI. These and yet uncharacterized T7SS transmembrane proteins merit further studies and could represent potential therapeutic targets.

scholarly journals Experimental, Clinical, and Morphological Analysis of H-Ras Oncoproteins for Locally Advanced Breast Cancer

2020 ◽  
Vol 8 (B) ◽  
pp. 1077-1082
Author(s):  
Ainura Maratovna Zhumakayeva ◽  
K. D. Rakhimov ◽  
I. M. Omarova ◽  
S. M. Adekenov ◽  
S. S. Zhumakayeva
Keyword(s):  
Breast Cancer ◽  
Comparative Analysis ◽  
Neoadjuvant Chemotherapy ◽  
Locally Advanced Breast Cancer ◽  
Locally Advanced ◽  
Survival Rates ◽  
Relapse Free Survival ◽  
Free Survival ◽  
Positive Expression ◽  
Egfr Expression

BACKGROUND: Activated forms of RAS increase both in breast cancer and in cell lines in the presence of estimated glomerular filtration rate (EGFR) or HER2 expression. HRAS oncoproteins play an important role in enhancing the proliferation and resistance of breast cancer tumor cells to apoptosis. A number of studies have shown a significant decrease in EGFR expression after neoadjuvant chemotherapy, which has been clinical, manifested by an improvement in immediate efficacy and an increase in overall and relapse-free breast cancer survival rates. AIM: The aim of the study was to study relapse-free survival depending on the expression of the H-RAS oncoprotein in patients with breast cancer who received different treatment regimens for the farnesyltransferase inhibitor. METHODS: H-RAS status was assessed by immunohistochemistry. RESULTS: A comparative analysis of patients with negative expression of H-RAS oncoproteins showed a statistically significant increase in relapse-free survival in the subgroups who received neoadjuvant chemotherapy according to the AC regimen (adriablastin + cyclophosphamide) and AC + arglabin, compared with monotherapy by arglabin: Kruskal–Wallis= 12.56, where p = 0.001. A comparative analysis of patients with positive expression of H-RAS showed that in the subgroups treated with arglabin and AC+arglabin, there was a statistically significant increase in relapse-free survival compared with the AC subgroup: Kruskal–Wallis = 10.96, where p = 0.004. It was established that the positive expression of H-RAS negatively affects not only the direct effectiveness of neoadjuvant therapy but also worsens the rates of relapse-free survival. However, in patients with positive H-RAS expression who received arglabin in monotherapy, there was a statistically significant increase in relapse-free survival up to 16.5 ± 1.1 months compared with the standard AC regimen (13.5 ± 1.1 months) (р ˂ 0.05), the addition of arglabin to the standard AC regime also increased this indicator to 16.4 ± 1.2 months (р ˂ 0.05). CONCLUSION: These results may indicate the clinical applicability of determining H-RAS as a prognostic factor for relapse-free survival in breast cancer.

scholarly journals An uncommon brain abscess caused by Streptococcus intermedius due to periodontal inflammation

2017 ◽  
Vol 18 (4) ◽  
pp. 254-257
Author(s):  
Vladimír Mihál ◽  
Marta Neklanová ◽  
Eva Klásková ◽  
David Krahulík ◽  
Kamila Michálková
Keyword(s):  
Brain Abscess ◽  
Streptococcus Intermedius ◽  
Periodontal Inflammation

scholarly journals A Novel Approach to Helicobacter pylori Pan-Genome Analysis for Identification of Genomic Islands

PLoS ONE ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. e0159419 ◽  
Author(s):  
Ikuo Uchiyama ◽  
Jacob Albritton ◽  
Masaki Fukuyo ◽  
Kenji K. Kojima ◽  
Koji Yahara ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Genome Analysis ◽  
Genomic Islands ◽  
Pan Genome ◽  
Novel Approach

scholarly journals The pan-genome of Treponema pallidum reveals differences in genome plasticity between subspecies related to venereal and non-venereal syphilis

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Arun Kumar Jaiswal ◽  
Sandeep Tiwari ◽  
Syed Babar Jamal ◽  
Letícia de Castro Oliveira ◽  
Leandro Gomes Alves ◽  
...  
Keyword(s):  
Treponema Pallidum ◽  
Genomic Islands ◽  
Personal Contact ◽  
Pathogenicity Islands ◽  
High Morbidity ◽  
Genome Plasticity ◽  
Acid Biosynthesis ◽  
Diverse Range ◽  
Pan Genome ◽  
Genomic Core

Abstract Background Spirochetal organisms of the Treponema genus are responsible for causing Treponematoses. Pathogenic treponemes is a Gram-negative, motile, spirochete pathogen that causes syphilis in human. Treponema pallidum subsp. endemicum (TEN) causes endemic syphilis (bejel); T. pallidum subsp. pallidum (TPA) causes venereal syphilis; T. pallidum subsp. pertenue (TPE) causes yaws; and T. pallidum subsp. Ccarateum causes pinta. Out of these four high morbidity diseases, venereal syphilis is mediated by sexual contact; the other three diseases are transmitted by close personal contact. The global distribution of syphilis is alarming and there is an increasing need of proper treatment and preventive measures. Unfortunately, effective measures are limited. Results Here, the genome sequences of 53 T. pallidum strains isolated from different parts of the world and a diverse range of hosts were comparatively analysed using pan-genomic strategy. Phylogenomic, pan-genomic, core genomic and singleton analysis disclosed the close connection among all strains of the pathogen T. pallidum, its clonal behaviour and showed increases in the sizes of the pan-genome. Based on the genome plasticity analysis of the subsets containing the subspecies T pallidum subsp. pallidum, T. pallidum subsp. endemicum and T. pallidum subsp. pertenue, we found differences in the presence/absence of pathogenicity islands (PAIs) and genomic islands (GIs) on subsp.-based study. Conclusions In summary, we identified four pathogenicity islands (PAIs), eight genomic islands (GIs) in subsp. pallidum, whereas subsp. endemicum has three PAIs and seven GIs and subsp. pertenue harbours three PAIs and eight GIs. Concerning the presence of genes in PAIs and GIs, we found some genes related to lipid and amino acid biosynthesis that were only present in the subsp. of T. pallidum, compared to T. pallidum subsp. endemicum and T. pallidum subsp. pertenue.

Brain Abscess Due To Aggregatibacter Aphrophilus and Streptococcus Intermedius: A Case Report

2017 ◽  
Author(s):  
Sevliya Öcal Demir ◽  
Eda Kepenekli Kadayifci ◽  
Gülşen Akkoç ◽  
Nurhayat Yakut ◽  
Adnan Dağçınar ◽  
...  
Keyword(s):  
Case Report ◽  
Brain Abscess ◽  
Streptococcus Intermedius ◽  
Aggregatibacter Aphrophilus

scholarly journals Comparative analysis of essential genes in prokaryotic genomic islands

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Xi Zhang ◽  
Chong Peng ◽  
Ge Zhang ◽  
Feng Gao
Keyword(s):  
Comparative Analysis ◽  
Essential Genes ◽  
Genomic Islands
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