scholarly journals Non-typhoidal Salmonella in the Pig Production Chain: A Comprehensive Analysis of Its Impact on Human Health

Pathogens ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 19 ◽  
Author(s):  
Joana Campos ◽  
Joana Mourão ◽  
Luísa Peixe ◽  
Patrícia Antunes
Keyword(s):  
Public Health ◽  
Antibiotic Resistance ◽  
Food Chain ◽  
Health Concern ◽  
Animal Origin ◽  
Pork Meat ◽  
Production Chain ◽  
Pig Production ◽  
Salmonella Serotypes ◽  
Human Infections

Salmonellosis remains one of the most frequent foodborne zoonosis, constituting a worldwide major public health concern. The most frequent sources of human infections are food products of animal origin, being pork meat one of the most relevant. Currently, particular pig food production well-adapted and persistent Salmonella enterica serotypes (e.g., Salmonella Typhimurium, Salmonella 1,4,[5],12:i:-, Salmonella Derby and Salmonella Rissen) are frequently reported associated with human infections in diverse industrialized countries. The dissemination of those clinically-relevant Salmonella serotypes/clones has been related to the intensification of pig production chain and to an increase in the international trade of pigs and pork meat. Those changes that occurred over the years along the food chain may act as food chain drivers leading to new problems and challenges, compromising the successful control of Salmonella. Among those, the emergence of antibiotic resistance in non-typhoidal Salmonella associated with antimicrobials use in the pig production chain is of special concern for public health. The transmission of pig-related multidrug-resistant Salmonella serotypes, clones and/or genetic elements carrying clinically-relevant antibiotic resistance genes, frequently associated with metal tolerance genes, from pigs and pork meat to humans, has been reported and highlights the contribution of different drivers to the antibiotic resistance burden. Gathered data strengthen the need for global mandatory interventions and strategies for effective Salmonella control and surveillance across the pig production chain. The purpose of this review was to provide an overview of the role of pig and pork meat in human salmonellosis at a global scale, highlighting the main factors contributing to the persistence and dissemination of clinically-relevant pig-related Salmonella serotypes and clones.

scholarly journals Degradation of antibiotic resistance genes and mobile gene elements in dairy manure anerobic digestion

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0254836
Author(s):  
Yi Wang ◽  
Pramod K. Pandey ◽  
Sundaram Kuppu ◽  
Richard Pereira ◽  
Sharif Aly ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Anaerobic Digestion ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Dairy Manure ◽  
Health Concern ◽  
Animal Origin ◽  
Rrna Gene ◽  
Transposase Gene ◽  
Integrase Gene

Antibiotic resistance genes (ARGs) are emerging contaminants causing serious global health concern. Interventions to address this concern include improving our understanding of methods for treating waste material of human and animal origin that are known to harbor ARGs. Anaerobic digestion is a commonly used process for treating dairy manure, and although effective in reducing ARGs, its mechanism of action is not clear. In this study, we used three ARGs to conducted a longitudinal bench scale anaerobic digestion experiment with various temperatures (28, 36, 44, and 52°C) in triplicate using fresh dairy manure for 30 days to evaluate the reduction of gene abundance. Three ARGs and two mobile genetic elements (MGEs) were studied: sulfonamide resistance gene (sulII), tetracycline resistance genes (tetW), macrolide-lincosamide-streptogramin B (MLSB) superfamily resistance genes (ermF), class 1 integrase gene (intI1), and transposase gene (tnpA). Genes were quantified by real-time quantitative PCR. Results show that the thermophilic anaerobic digestion (52°C) significantly reduced (p < 0.05) the absolute abundance of sulII (95%), intI1 (95%), tnpA (77%) and 16S rRNA gene (76%) after 30 days of digestion. A modified Collins–Selleck model was used to fit the decay curve, and results suggest that the gene reduction during the startup phase of anaerobic digestion (first 5 days) was faster than the later stage, and reductions in the first five days were more than 50% for most genes.

scholarly journals Antibiotics in Food Chain: The Consequences for Antibiotic Resistance

Antibiotics ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 688
Author(s):  
Shashi B. Kumar ◽  
Shanvanth R. Arnipalli ◽  
Ouliana Ziouzenkova
Keyword(s):  
Public Health ◽  
Antibiotic Resistance ◽  
Food Chain ◽  
Bacterial Infections ◽  
Antibiotic Resistance Genes ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Alternative Strategies ◽  
Antibiotic Resistant ◽  
Continuous Use

Antibiotics have been used as essential therapeutics for nearly 100 years and, increasingly, as a preventive agent in the agricultural and animal industry. Continuous use and misuse of antibiotics have provoked the development of antibiotic resistant bacteria that progressively increased mortality from multidrug-resistant bacterial infections, thereby posing a tremendous threat to public health. The goal of our review is to advance the understanding of mechanisms of dissemination and the development of antibiotic resistance genes in the context of nutrition and related clinical, agricultural, veterinary, and environmental settings. We conclude with an overview of alternative strategies, including probiotics, essential oils, vaccines, and antibodies, as primary or adjunct preventive antimicrobial measures or therapies against multidrug-resistant bacterial infections. The solution for antibiotic resistance will require comprehensive and incessant efforts of policymakers in agriculture along with the development of alternative therapeutics by experts in diverse fields of microbiology, biochemistry, clinical research, genetic, and computational engineering.

scholarly journals Isolation and molecular characterization of Arcobacter butzleri and Arcobacter cryaerophilus from the pork production chain in Brazil

2018 ◽  
Vol 38 (3) ◽  
pp. 393-399 ◽  
Author(s):  
Débora D.S. Gobbi ◽  
Maria G. Spindola ◽  
Luisa Z. Moreno ◽  
Carlos E.C. Matajira ◽  
Maria G.X. Oliveira ◽  
...  
Keyword(s):  
Animal Origin ◽  
Isolation And Identification ◽  
Production Chain ◽  
Fecal Samples ◽  
Pork Production ◽  
True Incidence ◽  
Arcobacter Butzleri ◽  
Meat Markets ◽  
Human Infections

ABSTRACT: Arcobacter is an emerging zoonotic pathogen, and the major transmission routes to humans are the handling or consumption of contaminated raw/undercooked food products of animal origin, water and seafood. The isolation and identification of Arcobacter species are not routine in clinical laboratories; therefore, its true incidence in human infections may be underestimated. The present study aimed to isolate and characterize Arcobacter from carcasses and fecal samples collected at swine slaughterhouses and from meat markets in São Paulo State, Brazil. The isolates were identified using multiplex-PCR to differentiate the species and analyzed by single-enzyme amplified fragment length polymorphism (SE-AFLP). Arcobacter spp. were isolated from 73.0% of swine carcasses, 4% of fecal samples and 10% of pork samples. A. butzleri was the most prevalent species identified, followed by A. cryaerophilus. Interestingly, the carcasses presented higher frequency of A. butzleri isolation, whereas only A. cryaerophilus was isolated from fecal samples. SE-AFLP enabled the characterization of A. butzleri and A. cryaerophilus into 51 and 63 profiles, respectively. The great genetic heterogeneity observed for both species corroborates previous reports. This study confirms the necessity for a standard isolation protocol and the improvement of molecular tools to further elucidate Arcobacter epidemiology.

scholarly journals Prevalence of Staphylococcus Aureus Isolated from Hamburger Samples in Tehran and their Antibiotic Susceptibility

2019 ◽  
Author(s):  
Negin Momtaz Bokharaei ◽  
Zahra Rajabi ◽  
MohammadMehdi Soltan Dallal
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Inhibitory Concentration ◽  
Food Poisoning ◽  
Health Concern ◽  
Diffusion Method ◽  
Public Health Concern ◽  
Disk Diffusion Method ◽  
Food Borne

Introduction:Staphylococcal food poisoning is one of the most common food-borne diseases. The antibiotic resistance of Staphylococcus aureus has been reported globally. Today, strains of Staphylococcus aureus in food have become a problem in clinical infections and are considerde as a serious public health concern. Methods: A total of 100 samples were tested in the laboratory; including 75 handmade and 25 industrial hamburger samples. The Staphylococcus aureus samples were isolated and identified according to the National Iranian Standard No. 6806-3. Antibiotic resistance was determined by disk diffusion method and minimum inhibitory concentration was performed. In this study, the antibiotics penicillin, ciprofloxacin, erythromycin, clindamycin, chloramphenicol, cefoxitin, trimethoprim-sulfamethoxazole, tetracycline, gentamicin, vancomycin, and oxacillin were used. Results: Of 100 analyzed hamburger samples, 39 samples were infected with Staphylococcus aureus (23 industrial and 16 ­ handmade samples). All isolates of Staphylococcus aureus (100%) were susceptible to gentamicin and vancomycin antibiotics. Conclusions: Due to the importance of Staphylococcus aureus, as the supergene antigen and its role in causing food poisoning, contamination of foodstuffs with Staphylococcus aureus can threaten the consumers' health.

scholarly journals Antimicrobial Resistance and Virulence Genes inEnterococcus faeciumandEnterococcus faecalisfrom Humans and Retail Red Meat

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Majda Golob ◽  
Mateja Pate ◽  
Darja Kušar ◽  
Urška Dermota ◽  
Jana Avberšek ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Red Meat ◽  
Health Concern ◽  
Animal Origin ◽  
Common Pattern ◽  
Exact Test ◽  
Microdilution Method ◽  
Food Of Animal Origin ◽  
Broth Microdilution Method ◽  
Human Infections

The emergence of antimicrobial-resistant and virulent enterococci is a major public health concern. While enterococci are commonly found in food of animal origin, the knowledge on their zoonotic potential is limited. The aim of this study was to determine and compare the antimicrobial susceptibility and virulence traits ofEnterococcus faecalisandEnterococcus faeciumisolates from human clinical specimens and retail red meat in Slovenia. A total of 242 isolates were investigated: 101 from humans (71E. faecalis, 30E. faecium) and 141 from fresh beef and pork (120E. faecalis, 21E. faecium). The susceptibility to 12 antimicrobials was tested using a broth microdilution method, and the presence of seven common virulence genes was investigated using PCR. In both species, the distribution of several resistance phenotypes and virulence genes was disparate for isolates of different origin. All isolates were susceptible to daptomycin, linezolid, teicoplanin, and vancomycin. In both species, the susceptibility to antimicrobials was strongly associated with a food origin and the multidrug resistance, observed in 29.6% ofE. faecalisand 73.3%E. faeciumclinical isolates, with a clinical origin (Fisher’s exact test). Among meat isolates, in total 66.0% ofE. faecalisandE. faeciumisolates were susceptible to all antimicrobials tested and 32.6% were resistant to either one or two antimicrobials. InE. faecalis, several virulence genes were significantly associated with a clinical origin; the most common (31.0%) gene pattern included all the tested genes excepthyl. In meat isolates, the virulence genes were detected inE. faecalisonly and the most common pattern includedace,efaA, andgelE(32.5%), of whichgelEshowed a statistically significant association with a clinical origin. These results emphasize the importance ofE. faecalisin red meat as a reservoir of virulence genes involved in its persistence and human infections with reported severe outcomes.

scholarly journals Analysis of foreign matter in foodstuffs using the light filth test: report 2012-2013

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Maria Giovanna Tilocca ◽  
Elisa Caneglias ◽  
Bruna Vodret ◽  
Maria Rosalba Mancuso ◽  
Antonina Zimmardi ◽  
...  
Keyword(s):  
Public Health ◽  
Veterinary Medicine ◽  
Food Chain ◽  
Food Production ◽  
Suitable Method ◽  
Animal Origin ◽  
Foreign Matter ◽  
Test Report ◽  
European Legislation ◽  
Public Health Protection

The European legislation set the new hygiene standards in food chain with the purpose to ensure high levels of public health protection in relation to food production. In order to guarantee excellent hygiene standards in food chain, particular attention must be paid to the presence of foreign matter, like light solid impurities of mineral, vegetable or animal origin. The light filth test is a suitable method used to detect and count light solid impurities applicable to different foodstuffs. We report the results of the analysis of 93 foodstuffs official samples investigated for the presence of foreign matter at the Institute for Experimental Veterinary Medicine of Sardinia, from 2012 to 2013. Insect fragments were found in a sample of semolina and in a sample of canned tomato; plastic fragments were found in a sample of grated bread.

Superbugs: Antibiotic Resistance Is Becoming a Major Public Health Concern

2016 ◽  
Vol 29 (2) ◽  
pp. 130-133 ◽  
Author(s):  
Patricia T. Alpert
Keyword(s):  
Public Health ◽  
Antibiotic Resistance ◽  
Clostridium Difficile ◽  
Current Treatment ◽  
Health Concern ◽  
Treatment Modalities ◽  
Public Health Concern ◽  
Carbapenem Resistant ◽  
Control And Prevention ◽  
Carbapenem Resistant Enterobacteriaceae

Antibiotic resistance has led to the development of so-called “superbugs” that no longer respond to the current treatment modalities. The array of antibiotics available to treat these infections is dwindling with very few antibiotics in the pipeline. This article discussed the pathogens the Centers for Disease Control and Prevention (CDC) has placed in their urgent category: (1) Clostridium difficile, (2) Carbapenem-resistant Enterobacteriaceae, and (3) Neisseria gonorrhoeae. In addition, a few suggestions for prevention of resistance are offered.

scholarly journals Integration of Mobile Devices and Point-Of-Care Diagnostic Devices—The Case of C-Reactive Protein Diagnosis

Diagnostics ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 181 ◽  
Author(s):  
Xin-Fang Wu ◽  
Ching-Fen Shen ◽  
Chao-Min Cheng
Keyword(s):  
Public Health ◽  
Antibiotic Resistance ◽  
Mobile Devices ◽  
Point Of Care ◽  
Health Concern ◽  
Global Public Health ◽  
C Reactive Protein ◽  
Public Health Concern ◽  
Reactive Protein

In recent years, the misuse and overuse of antibiotics has promoted antibiotic resistance, which has now become a global public health concern [...]

scholarly journals Determination of antibacterial residues in raw cattle beef in some selective areas of Mymensingh District during Eid-ul-Adha

2021 ◽  
Vol 18 (2) ◽  
Author(s):  
M. Comruzzaman ◽  
A. K. M. H. Kabir ◽  
A. Nahar ◽  
N. Hassan ◽  
M. M. Alam
Keyword(s):  
Public Health ◽  
High Performance ◽  
Health Concern ◽  
Animal Origin ◽  
Withdrawal Period ◽  
Drug Residue ◽  
The Mean ◽  
Public Health Hazards ◽  
Antibiotic Residue

Background: Antibiotic residue in foods of animal origin is a growing public health concern in recent time. Indiscriminate use of antibacterials in food animal production and ignoring proper withdrawal period may lead to drug residue in milk, beef and eggs. The present study was conducted to detect antibacterial residues in raw cattle beef samples. Methods: One hundred cattle beef samples were collected from four Upazillas of Mymensingh named Gofargaon, Fulpur, Dhobaura and Bhaluka, Bangladesh during the period of Eid-ul-adha in 2018. The residue of two most commonly used antibacterials named oxytetracycline (OTC) and sulphadimidin (SDI) were quantified by using High Performance Liquid Chromatography (HPLC). Results The mean concentration of OTC and SDI residues in raw beef samples were 0.145 µg/ml and 0.094 µg/ml and the concentration ranged from 0.104-0.435 µg/ml and 0.083-0.357 µg/ml, respectively. About 59% and 43% beef samples contained some levels of OTC and SDI residue, respectively. Importantly, 19% and 12% beef samples exceeded maximum residue limit (MRL) for OTC and SDI, respectively. Conclusions: The results indicate that drug residue is common in raw beef samples and a good proportion of beef samples exceeded MRL. Farmer’s education to ensure rational use of antibacterials and to follow withdrawal period may help control this public health hazards in Bangladesh context.

scholarly journals Antibiotic resistance profiles of Staphylococcus spp. from white button mushrooms and handlers

2021 ◽  
Vol 117 (11/12) ◽  
Author(s):  
Stacey Duvenage ◽  
Werner Rossouw ◽  
Germán Villamizar-Rodríguez ◽  
Erika M. du Plessis ◽  
Lise Korsten
Keyword(s):  
Public Health ◽  
Antibiotic Resistance ◽  
Opportunistic Infections ◽  
Meca Gene ◽  
Antibiotic Resistance Genes ◽  
Multidrug Resistant ◽  
Health Concern ◽  
Staphylococcus Spp ◽  
White Button Mushrooms ◽  
Potential Public Health

The presence of Staphylococcus spp. has increasingly been reported in food products and poses a public health threat. The aim of this study was to determine the diversity of Staphylococcus spp. and the antibiotic resistance profiles of isolates obtained from freshly harvested and packed ready-to-eat mushrooms (n=432) and handlers’ hands (n=150). A total of 56 Staphylococcus isolates [46.4% (n=26) from hands and 53.6% (n=30) from mushrooms] were recovered belonging to 10 species. Staphylococcus succinus isolates (n=21) were the most prevalent, of which 52.4% came from mushrooms and 47.6% from hands. This was followed by S. equorum isolates [n=12; 91.7% (n=11) from mushrooms and 8.3% (n=1) from hands] and S. saprophyticus [n=9; 66.7% (n=6) from mushrooms and 33.3% (n=3) from hands]. Six isolates that were characterised as multidrug resistant were isolated from hands of handlers. Most (83.9%; n=47) of the 56 isolates were resistant to penicillin [53.2% (n=25) from mushrooms and 46.8% (n=22) from hands] and 14.3% (n=8) were resistant to cephalosporin classes [25% (n=2) from mushrooms and 75% (n=6) from hands], both of which are used to treat staphylococcal infections. Antibiotic resistance genes blaZ [25.0% (n=14) of all isolates of which 71.4% (n=10) were from hands and 28.57% (n=4) from mushrooms], tetL and tetK [both 1.8% (n=1) from hands], mecA [5.4% (n=3) from hands] and ermA [1.8% (n=1) from mushrooms] were detected from the 56 isolates. Only two (25.0%) of the eight methicillin-resistant staphylococci harboured the mecA gene, while only 11 (23%) of the 47 penicillin-resistant isolates harboured the blaZ gene [36.4% (n=4) from mushrooms and 63.6% (n=7) from hands]. Our results demonstrate that food handlers and harvested and packed ready-to-eat mushrooms could be a source of diverse Staphylococcus spp. that exhibit antimicrobial resistance. Clinically relevant S. aureus was only detected on one handler’s hand; however, the isolate was not multidrug resistant. The presence of diverse Staphylococcus spp. on mushrooms and the hands of handlers is a potential public health concern due to their potential to cause opportunistic infections.

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