scholarly journals Omega-3 Polyunsaturated Fatty Acids Trigger Cell Cycle Arrest and Induce Apoptosis in Human Neuroblastoma LA-N-1 Cells

Nutrients ◽  
2015 ◽  
Vol 7 (8) ◽  
pp. 6956-6973 ◽  
Author(s):  
Wai So ◽  
Wai Liu ◽  
Kwok Leung
Keyword(s):  
Cell Cycle ◽  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Cell Cycle Arrest ◽  
Omega 3 ◽  
Human Neuroblastoma ◽  
Cycle Arrest

scholarly journals Omega-3 Polyunsaturated Fatty Acids Enhance Neuronal Differentiation in Cultured Rat Neural Stem Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Masanori Katakura ◽  
Michio Hashimoto ◽  
Toshiyuki Okui ◽  
Hossain Md Shahdat ◽  
Kentaro Matsuzaki ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Fatty Acids ◽  
Stem Cells ◽  
Polyunsaturated Fatty Acids ◽  
Neural Stem Cells ◽  
Cell Cycle Arrest ◽  
Neuronal Differentiation ◽  
Mrna Levels ◽  
Cycle Arrest ◽  
Hes1 Expression

Polyunsaturated fatty acids (PUFAs) can induce neurogenesis and recovery from brain diseases. However, the exact mechanisms of the beneficial effects of PUFAs have not been conclusively described. We recently reported that docosahexaenoic acid (DHA) induced neuronal differentiation by decreasing Hes1 expression and increasingp27kip1expression, which causes cell cycle arrest in neural stem cells (NSCs). In the present study, we examined the effect of eicosapentaenoic acid (EPA) and arachidonic acid (AA) on differentiation, expression of basic helix-loop-helix transcription factors (Hes1, Hes6, and NeuroD), and the cell cycle of cultured NSCs. EPA also increased mRNA levels of Hes1, an inhibitor of neuronal differentiation, Hes6, an inhibitor of Hes1, NeuroD, and Map2 mRNA and Tuj-1-positive cells (a neuronal marker), indicating that EPA induced neuronal differentiation. EPA increased the mRNA levels ofp21cip1andp27kip1, a cyclin-dependent kinase inhibitor, which indicated that EPA induced cell cycle arrest. Treatment with AA decreased Hes1 mRNA but did not affect NeuroD and Map2 mRNA levels. Furthermore, AA did not affect the number of Tuj-1-positive cells or cell cycle progression. These results indicated that EPA could be involved in neuronal differentiation by mechanisms alternative to those of DHA, whereas AA did not affect neuronal differentiation in NSCs.

Activation of the BRCA1/Chk1/p53/p21Cip1/Waf1 pathway by nitric oxide and cell cycle arrest in human neuroblastoma NB69 cells

Nitric Oxide ◽  
2012 ◽  
Vol 26 (3) ◽  
pp. 182-191 ◽  
Author(s):  
Marlies Van de Wouwer ◽  
Célia Couzinié ◽  
Miguel Serrano-Palero ◽  
Óscar González-Fernández ◽  
Clara Galmés-Varela ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Human Neuroblastoma ◽  
Cycle Arrest

MCPIP1 overexpression in human neuroblastoma cell lines causes cell‐cycle arrest by G1/S checkpoint block

2020 ◽  
Vol 121 (5-6) ◽  
pp. 3406-3425 ◽  
Author(s):  
Elżbieta Boratyn ◽  
Iwona Nowak ◽  
Elżbieta Karnas ◽  
Damian Ryszawy ◽  
Dawid Wnuk ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Cycle Arrest ◽  
Neuroblastoma Cell Lines

scholarly journals Key role for p27Kip1, retinoblastoma protein Rb, and MYCN in polyamine inhibitor-induced G1 cell cycle arrest in MYCN-amplified human neuroblastoma cells

Oncogene ◽  
2005 ◽  
Vol 24 (36) ◽  
pp. 5606-5618 ◽  
Author(s):  
Christopher J Wallick ◽  
Ivonne Gamper ◽  
Mike Thorne ◽  
David J Feith ◽  
Kelsie Y Takasaki ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Neuroblastoma Cells ◽  
Retinoblastoma Protein ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Cycle Arrest ◽  
G1 Cell Cycle Arrest

scholarly journals Anti-Tumor Effect of Rutin on Human Neuroblastoma Cell Lines through Inducing G2/M Cell Cycle Arrest and Promoting Apoptosis

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Hongyan Chen ◽  
Qing Miao ◽  
Miao Geng ◽  
Jing Liu ◽  
Yazhuo Hu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cell Apoptosis ◽  
Cell Cycle Progression ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Human Neuroblastoma ◽  
Cycle Progression ◽  
Cycle Arrest ◽  
Flow Cytometric

Aims. To further investigate the antineuroblastoma effect of rutin which is a type of flavonoid.Methods. The antiproliferation of rutin in human neuroblastoma cells LAN-5 were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Chemotaxis of LAN-5 cells was assessed using transwell migration chambers and scratch wound migration assay. The cell cycle arrest and apoptosis in a dose-dependent manner was measured by flow cytometric and fluorescent microscopy analyses. The apoptosis-related proteins BAX and BCL2 as well as MYCN mRNA express were determined by RT-PCR analysis. Secreted TNF-αlevel were determined using specific enzyme-linked immunosorbent assay kits.Results. Rutin significantly inhibited the growth of LAN-5 cells and chemotactic ability. Flow cytometric analysis revealed that rutin induced G2/M arrest in the cell cycle progression and induced cell apoptosis. The RT-PCR showed that rutin could decrease BCL2 expression and BCL2/BAX ratio. In the meantime, the MYCN mRNA level and the secretion of TNF-αwere inhibited.Conclusion. These results suggest that rutin produces obvious antineuroblastoma effects via induced G2/M arrest in the cell cycle progression and induced cell apoptosis as well as regulating the expression of gene related to apoptosis and so on. It supports the viability of developing rutin as a novel therapeutic prodrug for neuroblastoma treatment, as well as providing a new path on anticancer effect of Chinese traditional drug.

Guanine nucleotide depletion triggers cell cycle arrest and apoptosis in human neuroblastoma cell lines

2003 ◽  
Vol 108 (6) ◽  
pp. 812-817 ◽  
Author(s):  
Elisa Messina ◽  
Paola Gazzaniga ◽  
Vanna Micheli ◽  
Maria Rosaria Guaglianone ◽  
Silvia Barbato ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cell Lines ◽  
Neuroblastoma Cell ◽  
Human Neuroblastoma Cell ◽  
Guanine Nucleotide ◽  
Human Neuroblastoma ◽  
Cycle Arrest ◽  
Neuroblastoma Cell Lines
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