scholarly journals Effects of Eicosapentaenoic Acid and Docosahexaenoic Acid on Uncoupling Protein 3 Gene Expression in C2C12 Muscle Cells

Nutrients ◽  
2013 ◽  
Vol 5 (5) ◽  
pp. 1660-1671 ◽  
Author(s):  
Mak-Soon Lee ◽  
In-Hwan Kim ◽  
Yangha Kim
Keyword(s):  
Gene Expression ◽  
Docosahexaenoic Acid ◽  
Eicosapentaenoic Acid ◽  
Uncoupling Protein ◽  
Muscle Cells ◽  
Uncoupling Protein 3 ◽  
C2c12 Muscle Cells

scholarly journals Up-Regulation of Uncoupling Protein 3 Gene Expression by Fatty Acids and Agonists for PPARs in L6 Myotubes

Endocrinology ◽  
2001 ◽  
Vol 142 (10) ◽  
pp. 4189-4194 ◽  
Author(s):  
Cheol Son ◽  
Kiminori Hosoda ◽  
Junichi Matsuda ◽  
Junji Fujikura ◽  
Shin Yonemitsu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
Uncoupling Protein ◽  
L6 Myotubes ◽  
Uncoupling Protein 3

scholarly journals 225 Effects of late gestation Calcium salts of fatty acids supplementation to beef cows on offspring pre-weaning growth performance and gene expression

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 136-136
Author(s):  
Taoqi Shao ◽  
Frank Ireland ◽  
Joshua Mccann ◽  
Daniel W Shike
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Eicosapentaenoic Acid ◽  
Growth Performance ◽  
Treatment Time ◽  
Beef Cows ◽  
Late Gestation ◽  
Time Interaction ◽  
Subcutaneous Adipose

Abstract Objectives were to investigate the effects of supplementing Ca salts of fatty acids to beef cows during late gestation on the pre-weaning growth performance and gene expression of steer progeny. Ninety-six fall-calving, Angus x Simmental cows (BW = 601±76 kg) were randomly assigned into 8 pastures (4 pastures/treatment; 12 cows/pasture), supplemented soyhulls mixed with 80 g/cow/d Strata + 80 g/cow/d Prequel (PUFA, rich in linoleic acid, eicosapentaenoic acid, and docosahexaenoic acid) or 155 g/cow/d EnerGII (SFA/MUFA, rich in palmitic and oleic acids) for 76.5±6.0 d prepartum. Cow/calf pairs were comingled after calving. Biopsies of Longissimus dorsi (LD) and subcutaneous adipose were taken at birth and 3 wk before weaning (186±6 d). The data were analyzed using the MIXED procedure of SAS. Supplementing PUFA increased (P < 0.01) cow plasma eicosapentaenoic acid and docosahexaenoic acid at midpoint of supplementation. At birth, PUFA steers had greater (P < 0.05) plasma EPA and lower (P < 0.01) plasma linolenic acid and oleic acid than SFA/MUFA steers. Weaning BW and pre-weaning ADG of the SFA/MUFA steers tended (P < 0.07) to be greater than PUFA steers. SFA/MUFA steers had increased (P = 0.04) expression of Zfp423 in adipose. There tended to be a treatment×time interaction (P = 0.06) for expression of Myf5 in LD; expression of Myf5 was greatest in SFA/MUFA steers at birth. There was a treatment×time interaction (P = 0.05) for expression of Pax7; expression was greater in SFA/MUFA steers at birth than any treatment at weaning. There were treatment×time interactions (P < 0.01) for expression of Myh7 and C/EBPβ in LD; expressions were greatest in PUFA steers at weaning. Supplementing Ca salts of PUFA to beef cows during late gestation altered plasma fatty acid profile of cow and calf, affected gene expression, and decreased offspring growth performance.

scholarly journals Uncoupling protein-3 gene expression: reduced skeletal muscle mRNA in obese humans during pronounced weight loss

Diabetologia ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 302-309 ◽  
Author(s):  
H. Esterbauer ◽  
H. Oberkofler ◽  
G. Dallinger ◽  
D. Breban ◽  
E. Hell ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Weight Loss ◽  
Uncoupling Protein ◽  
Uncoupling Protein 3

scholarly journals Thyroid hormones directly activate the expression of the human and mouse uncoupling protein-3 genes through a thyroid response element in the proximal promoter region

2005 ◽  
Vol 386 (3) ◽  
pp. 505-513 ◽  
Author(s):  
Gemma SOLANES ◽  
Neus PEDRAZA ◽  
Verónica CALVO ◽  
Antonio VIDAL-PUIG ◽  
Bradford B. LOWELL ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Thyroid Hormone ◽  
Thyroid Hormones ◽  
Uncoupling Protein ◽  
Gene Promoter ◽  
Proximal Promoter ◽  
Ucp3 Gene ◽  
Uncoupling Protein 3

The transcription of the human UCP3 (uncoupling protein-3) gene in skeletal muscle is tightly regulated by metabolic signals related to fatty acid availability. However, changes in thyroid status also modulate UCP3 gene expression, albeit by unknown mechanisms. We created transgenic mice bearing the entire human UCP3 gene to investigate the effect of thyroid hormones on human UCP3 gene expression. Treatment of human UCP3 transgenic mice with thyroid hormones induced the expression of the human gene in skeletal muscle. In addition, transient transfection experiments demonstrate that thyroid hormones activate the transcription of the human UCP3 gene promoter when MyoD and the TR (thyroid hormone receptor) were co-transfected. The action of thyroid hormones on UCP3 gene transcription is mediated by the binding of the TR to a proximal region in the UCP3 gene promoter that contains a direct repeat structure. An intact DNA sequence of this site is required for thyroid hormone responsiveness and TR binding. Chromatin immunoprecipitation assays revealed that the TR binds this element in vivo. The murine Ucp3 gene promoter was also dependent on MyoD and responsive to thyroid hormone in transient transfection assays. However, it was much less sensitive to thyroid hormone than the human UCP3 promoter. In summary, UCP3 gene transcription is activated by thyroid hormone treatment in vivo, and this activation is mediated by a TRE (thyroid hormone response element) in the proximal promoter region. Such regulation suggests a link between UCP3 gene expression and the effects of thyroid hormone on mitochondrial function in skeletal muscle.

Sign in / Sign up

Export Citation Format

Share Document