scholarly journals Differential and Synergistic Effects of Low Birth Weight and Western Diet on Skeletal Muscle Vasculature, Mitochondrial Lipid Metabolism and Insulin Signaling in Male Guinea Pigs

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4315
Author(s):  
Kristyn Dunlop ◽  
Ousseynou Sarr ◽  
Nicole Stachura ◽  
Lin Zhao ◽  
Karen Nygard ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lipid Metabolism ◽  
Birth Weight ◽  
Amino Acid ◽  
Low Birth Weight ◽  
Insulin Signaling ◽  
Western Diet ◽  
High Fat ◽  
High Sugar ◽  
Mitochondrial Lipid

Low birth weight (LBW) offspring are at increased risk for developing insulin resistance, a key precursor in metabolic syndrome and type 2 diabetes mellitus. Altered skeletal muscle vasculature, extracellular matrix, amino acid and mitochondrial lipid metabolism, and insulin signaling are implicated in this pathogenesis. Using uteroplacental insufficiency (UPI) to induce intrauterine growth restriction (IUGR) and LBW in the guinea pig, we investigated the relationship between UPI-induced IUGR/LBW and later life skeletal muscle arteriole density, fibrosis, amino acid and mitochondrial lipid metabolism, markers of insulin signaling and glucose uptake, and how a postnatal high-fat, high-sugar “Western” diet (WD) modulates these changes. Muscle of 145-day-old male LBW glucose-tolerant offspring displayed diminished vessel density and altered acylcarnitine levels. Disrupted muscle insulin signaling despite maintained whole-body glucose homeostasis also occurred in both LBW and WD-fed male “lean” offspring. Additionally, postnatal WD unmasked LBW-induced impairment of mitochondrial lipid metabolism, as reflected by increased acylcarnitine accumulation. This study provides evidence that early markers of skeletal muscle metabolic dysfunction appear to be influenced by the in utero environment and interact with a high-fat/high-sugar postnatal environment to exacerbate altered mitochondrial lipid metabolism, promoting mitochondrial overload.

scholarly journals Differential and synergistic effects of low birth weight and Western diet on skeletal muscle vasculature, mitochondrial lipid metabolism and insulin signaling in male guinea pigs

2021 ◽  
Author(s):  
Kristyn Dunlop ◽  
Ousseynou Sarr ◽  
Nicole Stachura ◽  
Lin Zhao ◽  
Karen Nygard ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lipid Metabolism ◽  
Birth Weight ◽  
Amino Acid ◽  
Low Birth Weight ◽  
Insulin Signaling ◽  
Western Diet ◽  
Whole Body ◽  
High Fat ◽  
Mitochondrial Lipid

Low birth weight (LBW) offspring are at increased risk for developing insulin resistance, a key precursor in metabolic syndrome and type 2 diabetes mellitus. Altered skeletal muscle vasculature, extracellular matrix, amino acid and mitochondrial lipid metabolism, and insulin signaling are implicated in this pathogenesis. Using uteroplacental insufficiency (UPI) to induce intrauterine growth restriction (IUGR) and LBW in the guinea pig, we investigated the relationship between UPI-induced IUGR/LBW and later life skeletal muscle arteriole density, fibrosis, amino acid and mitochondrial lipid metabolism, markers of insulin signaling and glucose uptake, and how a postnatal high-fat, high-sugar Western diet (WD) modulates these changes. Muscle of 145-day-old male LBW glucose tolerant offspring displayed diminished vessel density and altered acylcarnitine levels. Disrupted muscle insulin signaling despite maintained whole-body glucose homeostasis also occurred in both LBW and WD-fed male lean offspring. Additionally, postnatal WD unmasked LBW-induced impairment of mitochondrial lipid metabolism as reflected by in-creased acylcarnitine accumulation. This study provides evidence that early markers of skeletal muscle metabolic dysfunction appear to be influenced by the in utero environment and interact with a high fat-sugar postnatal environment to exacerbate altered mitochondrial lipid metabolism promoting mitochondrial overload.

scholarly journals Plasma amino acid levels are elevated in young, healthy low birth weight men exposed to short-term high-fat overfeeding

2016 ◽  
Vol 4 (23) ◽  
pp. e13044 ◽  
Author(s):  
Amalie Ribel-Madsen ◽  
Lars I. Hellgren ◽  
Charlotte Brøns ◽  
Rasmus Ribel-Madsen ◽  
Christopher B. Newgard ◽  
...  
Keyword(s):  
Birth Weight ◽  
Amino Acid ◽  
Low Birth Weight ◽  
Plasma Amino Acid ◽  
High Fat ◽  
Short Term ◽  
Amino Acid Levels

scholarly journals Transcript profile of skeletal muscle lipid metabolism genes affected by diet in a piglet model of low birth weight

PLoS ONE ◽  
2019 ◽  
Vol 14 (10) ◽  
pp. e0224484
Author(s):  
Quentin L. Sciascia ◽  
Gürbüz Daş ◽  
Steffen Maak ◽  
Claudia Kalbe ◽  
Barbara U. Metzler-Zebeli ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lipid Metabolism ◽  
Birth Weight ◽  
Low Birth Weight ◽  
Transcript Profile ◽  
Muscle Lipid ◽  
Skeletal Muscle Lipid ◽  
Lipid Metabolism Genes

Adiponectin resistance precedes the accumulation of skeletal muscle lipids and insulin resistance in high-fat-fed rats

2009 ◽  
Vol 296 (2) ◽  
pp. R243-R251 ◽  
Author(s):  
Kerry L. Mullen ◽  
Janet Pritchard ◽  
Ian Ritchie ◽  
Laelie A. Snook ◽  
Adrian Chabowski ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Lipid Metabolism ◽  
Protein Kinase ◽  
Glucose Transport ◽  
Insulin Signaling ◽  
Lipid Accumulation ◽  
Time Course ◽  
Protein Kinase B ◽  
High Fat

High-fat (HF) diets can induce insulin resistance (IR) by altering skeletal muscle lipid metabolism. An imbalance between fatty acid (FA) uptake and oxidation results in intramuscular lipid accumulation, which can impair the insulin-signaling cascade. Adiponectin (Ad) is an insulin-sensitizing adipokine known to stimulate skeletal muscle FA oxidation and reduce lipid accumulation. Evidence of Ad resistance has been shown in obesity and following chronic HF feeding and may contribute to lipid accumulation observed in these conditions. Whether Ad resistance precedes and is associated with the development of IR is unknown. We conducted a time course HF feeding trial for 3 days, 2 wk, or 4 wk to determine the onset of Ad resistance and identify the ensuing changes in lipid metabolism and insulin signaling leading to IR in skeletal muscle. Ad stimulated FA oxidation (+28%, P ≤ 0.05) and acetyl-CoA carboxylase phosphorylation (+34%, P ≤ 0.05) in control animals but failed to do so in any HF-fed group (i.e., as early as 3 days). By 2 wk, plasma membrane FA transporters and intramuscular diacylglycerol (DAG) and ceramide were increased, and insulin-stimulated phosphorylation of both protein kinase B and protein kinase B substrate 160 was blunted compared with control animals. After 4 wk of HF feeding, maximal insulin-stimulated glucose transport was impaired compared with control. Taken together, our results demonstrate that an early loss of Ad's stimulatory effect on FA oxidation precedes an increase in plasmalemmal FA transporters and the accumulation of intramuscular DAG and ceramide, blunted insulin signaling, and ultimately impaired maximal insulin-stimulated glucose transport in skeletal muscle induced by HF diets.

Enhanced nutrient supply to very low birth weight infants is associated with higher blood amino acid concentrations and improved growth

2017 ◽  
Vol 18 ◽  
pp. 16-22 ◽  
Author(s):  
Kenneth Strømmen ◽  
Alexander Haag ◽  
Sissel J. Moltu ◽  
Marit B. Veierød ◽  
Elin W. Blakstad ◽  
...  
Keyword(s):  
Birth Weight ◽  
Amino Acid ◽  
Low Birth Weight ◽  
Very Low Birth Weight ◽  
Nutrient Supply ◽  
Low Birth Weight Infants ◽  
Amino Acid Concentrations

Myostatin expression in porcine tissues: tissue specificity and developmental and postnatal regulation

1998 ◽  
Vol 275 (4) ◽  
pp. R1265-R1273 ◽  
Author(s):  
Shaoquan Ji ◽  
R. L. Losinski ◽  
S. G. Cornelius ◽  
G. R. Frank ◽  
G. M. Willis ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Mammary Gland ◽  
Birth Weight ◽  
Low Birth Weight ◽  
Tissue Specificity ◽  
Muscle Growth ◽  
Mrna Abundance ◽  
Longissimus Muscle ◽  
Gland Development ◽  
Level 2

The objective of this study was to establish the developmental pattern and tissue specificity of porcine myostatin expression and to evaluate expression in skeletal muscle during circumstances in which muscle growth was altered. Northern blot analysis revealed two transcripts (1.5 and 0.8 kb). Myostatin mRNA was detected in whole fetuses at 21 and 35 days and was markedly increased ( P < 0.05) by 49 days. At birth, mRNA abundance in longissimus muscle had declined significantly ( P < 0.05) from that at day 105 of gestation and continued to decrease ( P < 0.05) to its lowest level 2 wk postnatally (4 kg body wt). Myostatin expression was higher ( P < 0.05) at 55, 107, and 162 kg body wt than at 4 kg body wt. Postnatally, myostatin mRNA was detected in skeletal muscle and mammary gland. Expression at birth was 65% higher ( P < 0.04) in longissimus muscle of low-birth-weight piglets (0.57 ± 0.052 kg body wt) vs. normal (1.37 ± 0.077 kg body wt) littermates, irrespective of gender. However, suppression of longissimus muscle growth by food deprivation (3 days) did not alter ( P > 0.15) myostatin expression in either 4- or 7-wk-old piglets. Additionally, myostatin mRNA abundance was not changed by porcine growth hormone administration in growing animals. These data indicate that myostatin expression in skeletal muscle peaks prenatally and that greater expression is associated with low birth weight. Expression in mammary gland indicates a possible role for myostatin in mammary gland development and/or lactation.

scholarly journals Intramyocellular Lipid Accumulation After High-Fat Diet Is Associated with the Gene Expression Involved in Lipid Metabolism in Skeletal Muscle of Non-Obese Men

2016 ◽  
Vol 62 (Suppl.1) ◽  
pp. 144-145
Author(s):  
SAORI KAKEHI ◽  
YOSHIFUMI TAMURA ◽  
KAGEUMI TAKENO ◽  
YUKO SAKURAI ◽  
MINAKO KAWAGUCHI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Lipid Metabolism ◽  
Lipid Accumulation ◽  
High Fat Diet ◽  
Intramyocellular Lipid ◽  
High Fat
Sign in / Sign up

Export Citation Format

Share Document