scholarly journals Administration of Lactobacillus reuteri Combined with Clostridium butyricum Attenuates Cisplatin-Induced Renal Damage by Gut Microbiota Reconstitution, Increasing Butyric Acid Production, and Suppressing Renal Inflammation

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2792
Author(s):  
Yu-Ping Hsiao ◽  
Hsiao-Ling Chen ◽  
Jen-Ning Tsai ◽  
Meei-Yn Lin ◽  
Jiunn-Wang Liao ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Butyric Acid ◽  
Acid Production ◽  
Lactobacillus Reuteri ◽  
Intestinal Barrier ◽  
Toxin Production ◽  
Clostridium Butyricum ◽  
Uremic Toxin ◽  
Renal Inflammation ◽  
Butyric Acid Production

Cisplatin-induced nephrotoxicity is associated with gut microbiota disturbance. The present study aimed to investigate whether supplementation of Lactobacillus reuteri and Clostridium butyricum (LCs) had a protective effect on cisplatin-induced nephrotoxicity through reconstruction of gut microbiota. Wistar rats were given different treatments: control, cisplatin (Cis), cisplatin + C. butyricum and L. reuteri (Cis+LCs), and C. butyricum and L. reuteri (LCs). We observed that cisplatin-treated rats supplemented with LCs exhibited significantly decreased renal inflammation (KIM-1, F4/80, and MPO), oxidative stress, fibrosis (collagen IV, fibronectin, and a-SMA), apoptosis, concentration of blood endotoxin and indoxyl sulfate, and increased fecal butyric acid production compared with those without supplementation. In addition, LCs improved the cisplatin-induced microbiome dysbiosis by maintaining a healthy gut microbiota structure and diversity; depleting Escherichia-Shigella and the Enterobacteriaceae family; and enriching probiotic Bifidobacterium, Ruminococcaceae, Ruminiclostridium_9, and Oscillibacter. Moreover, the LCs intervention alleviated the cisplatin-induced intestinal epithelial barrier impairment. This study indicated LCs probiotic serves as a mediator of the gut–kidney axis in cisplatin-induced nephrotoxicity to restore the intestinal microbiota composition, thereby suppressing uremic toxin production and enhancing butyrate production. Furthermore, the renoprotective effect of LCs is partially mediated by increasing the anti-inflammatory effects and maintaining the integrity of the intestinal barrier.

Butyric acid production by Clostridium butyricum with integrated extraction and pertraction

1999 ◽  
Vol 34 (8) ◽  
pp. 835-843 ◽  
Author(s):  
Jana Zigová ◽  
Ernest Šturdı́k ◽  
Dušan Vandák ◽  
Štefan Schlosser
Keyword(s):  
Butyric Acid ◽  
Acid Production ◽  
Clostridium Butyricum ◽  
Butyric Acid Production ◽  
Integrated Extraction

A novel isolate of Clostridium butyricum for efficient butyric acid production by xylose fermentation

2018 ◽  
Vol 68 (6) ◽  
pp. 321-330
Author(s):  
Xin Wang ◽  
Jianzheng Li ◽  
Xue Chi ◽  
Yafei Zhang ◽  
Han Yan ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Acid Production ◽  
Xylose Fermentation ◽  
Clostridium Butyricum ◽  
Butyric Acid Production

High-Selectivity Butyric Acid Production from Saccharina japonica Hydrolysate by Clostridium tyrobutyricum

2020 ◽  
Vol 59 (39) ◽  
pp. 17147-17155 ◽  
Author(s):  
Hongxin Fu ◽  
Jialei Hu ◽  
Xiaolong Guo ◽  
Jun Feng ◽  
Yanan Zhang ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Acid Production ◽  
High Selectivity ◽  
Saccharina Japonica ◽  
Clostridium Tyrobutyricum ◽  
Butyric Acid Production

scholarly journals The Association between Gut Microbiota and Uremia of Chronic Kidney Disease

2020 ◽  
Vol 8 (6) ◽  
pp. 907 ◽  
Author(s):  
Ji Eun Kim ◽  
Hyo-Eun Kim ◽  
Ji In Park ◽  
Hyunjeong Cho ◽  
Min-Jung Kwak ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Gut Microbiota ◽  
Kidney Function ◽  
Kidney Diseases ◽  
Toxin Production ◽  
Positive Interactions ◽  
Uremic Toxin ◽  
Serum Concentrations ◽  
Pyruvate Metabolism

Chronic kidney disease (CKD)-associated uremia aggravates—and is aggravated by—gut dysbiosis. However, the correlation between CKD severity and gut microbiota and/or their uremic metabolites is unclear. We enrolled 103 CKD patients with stage 1 to 5 and 46 healthy controls. We analyzed patients’ gut microbiota by MiSeq system and measured the serum concentrations of four uremic metabolites (p-cresyl sulfate, indoxyl sulfate, p-cresyl glucuronide, and trimethylamine N-oxide) by liquid chromatography–tandem mass spectrometry. Serum concentrations of the uremic metabolites increased with kidney function deterioration. Gut microbial diversity did not differ among the examined patient and control groups. In moderate or higher stage CKD groups, Oscillibacter showed positive interactions with other microbiota, and the proportions of Oscillibacter were positively correlated with those of the uremic metabolites. The gut microbiota, particularly Oscillibacter, was predicted to contribute to pyruvate metabolism which increased with CKD progression. Relative abundance of Oscillibacter was significantly associated with both serum uremic metabolite levels and kidney function. Predicted functional analysis suggested that kidney-function-associated changes in the contribution of Oscillibacter to pyruvate metabolism in CKD may greatly affect the gut environment according to kidney function, resulting in dysbiosis concomitant with uremic toxin production. The gut microbiota could be associated with uremia progression in CKD. These results may provide basis for further metagenomics analysis of kidney diseases.

scholarly journals Probiotics Prevents Sensitization to Oral Antigen and Subsequent Increases in Intestinal Tight Junction Permeability in Juvenile–Young Adult Rats

2019 ◽  
Vol 7 (10) ◽  
pp. 463 ◽  
Author(s):  
Tulyeu ◽  
Kumagai ◽  
Jimbo ◽  
Watanabe ◽  
Yokoyama ◽  
...  
Keyword(s):  
Food Allergy ◽  
Young Adult ◽  
Gut Microbiota ◽  
Tight Junction ◽  
Intestinal Permeability ◽  
Lactobacillus Reuteri ◽  
Intestinal Barrier ◽  
Mucosal Inflammation ◽  
Adult Rats ◽  
Epithelial Barrier Function

Increased intestinal permeability is thought to underlie the pathogenesis of food allergy. We explore the mechanism responsible for changes in the morphology and function of the intestinal barrier using a rat model of food allergy, focusing on the contribution of intestinal microbiota. Juvenile–young adult rats were sensitized with ovalbumin and treated with antibiotics or probiotics (Clostridium butyricum and Lactobacillus reuteri), respectively. The serum ovalbumin-IgE levels, intestinal permeability, histopathological features, tight junction (TJ)-associated proteins, Th2 cytokines, and gut microbiota in feces were analyzed in each group. Sensitized rats showed an increase in ovalbumin-IgE levels and intestinal permeability with gut mucosal inflammation, whereas rats that received probiotics were only mildly affected. Rats given ovalbumin, but not those given probiotics, showed a reduction in both TJ-related protein expression and localization. Th2 cytokine levels were increased in the sensitized rats, but not in those given probiotics. TJs in rats treated with ovalbumin and antibiotics were disrupted, but those in rats administered probiotics were undamaged. Clostridiaceae were increased in the probiotics groups, especially Alkaliphilus, relative to the ovalbumin-sensitized group. Gut microbiota appears to play a role in regulating epithelial barrier function, and probiotics may help to prevent food sensitization through the up-regulation of TJ proteins.

Sign in / Sign up

Export Citation Format

Share Document