scholarly journals Improvement of Cutaneous Wound Healing via Topical Application of Heat-Killed Lactococcus chungangensis CAU 1447 on Diabetic Mice

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2666
Author(s):  
YoHan Nam ◽  
Jong-Hwa Kim ◽  
Jihye Baek ◽  
Wonyong Kim
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Therapeutic Agents ◽  
Skin Wound ◽  
Skin Microbiome ◽  
Cutaneous Wound Healing ◽  
Diabetic Mice ◽  
Cutaneous Wound ◽  
Beneficial Effects ◽  
Systemic Network

Cutaneous wound healing comprises a complex systemic network. Probiotics, naturally extracted substances, medicine, and chemical compounds have been used for wound healing, but the application of postbiotics as therapeutic agents has yet to be explored. Our study shows potential beneficial effects of heat-killed Lactococcus chungangensis CAU 1447 on type 1 diabetic mice. The postbiotic strain significantly decreased the skin wound size. The activity of myeloperoxidase secreted from neutrophils also decreased. The molecular mechanism of wound healing was adjusted by important mediators, growth factors, chemokines, and cytokines. These elements regulated the anti-inflammatory activity and accelerated wound healing. To determine the role of the postbiotic in wound repair, we showed a similar taxonomic pattern as compared to the diabetic mice using skin microbiome analysis. These findings demonstrated that heat-killed Lactococcus chungangensis CAU 1447 had beneficial effects on wound healing and can be utilized as postbiotic therapeutic agents.

scholarly journals Beneficial Effects of Deoxyshikonin on Delayed Wound Healing in Diabetic Mice

2018 ◽  
Vol 19 (11) ◽  
pp. 3660 ◽  
Author(s):  
Jun Park ◽  
Myoung-Sook Shin ◽  
Gwi Hwang ◽  
Noriko Yamabe ◽  
Jeong-Eun Yoo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Umbilical Vein ◽  
Sesame Seed ◽  
Tube Formation ◽  
Skin Wound ◽  
Diabetic Mice ◽  
Impaired Wound Healing ◽  
Beneficial Effects ◽  
Wide Range

Shiunko ointment is composed of five ingredients including Lithospermi Radix (LR), Angelicae Gigantis Radix, sesame seed oil, beeswax, and swine oil. It is externally applied as a treatment for a wide range of skin conditions such as eczema, psoriasis, hair loss, burns, topical wounds, and atopic dermatitis. Deoxyshikonin is the major angiogenic compound extracted from LR. In this study, we investigated the efficacy of LR extract and deoxyshikonin on impaired wound healing in streptozotocin (STZ)-induced diabetic mice. Treatment with LR extract elevated tube formation in human umbilical vein endothelial cells (HUVECs) and exerted antioxidant activity. An open skin wound was produced on the backs of diabetic mice and was then topically treated with deoxyshikonin or vehicle. In addition, deoxyshikonin promoted tube formation in high glucose conditions exposed to HUVECs, and which may be regulated by increased VEGFR2 expression and phosphorylation of Akt and p38. Our results demonstrate that deoxyshikonin application promoted wound repair in STZ-induced diabetic mice. Collectively, these data suggest that deoxyshikonin is an active ingredient of LR, thereby contributing to wound healing in patients with diabetes.

scholarly journals Comparison of EGF with VEGF Non-Viral Gene Therapy for Cutaneous Wound Healing of Streptozotocin Diabetic Mice

2011 ◽  
Vol 35 (3) ◽  
pp. 226 ◽  
Author(s):  
Junghae Ko ◽  
Haejung Jun ◽  
Hyesook Chung ◽  
Changshin Yoon ◽  
Taekyoon Kim ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Wound Healing ◽  
Viral Gene ◽  
Cutaneous Wound Healing ◽  
Diabetic Mice ◽  
Cutaneous Wound ◽  
Viral Gene Therapy

scholarly journals Activated Protein C in Cutaneous Wound Healing: From Bench to Bedside

2019 ◽  
Vol 20 (4) ◽  
pp. 903 ◽  
Author(s):  
Ruilong Zhao ◽  
Haiyan Lin ◽  
Lara Bereza-Malcolm ◽  
Elizabeth Clarke ◽  
Christopher Jackson ◽  
...  
Keyword(s):  
Wound Healing ◽  
Clinical Studies ◽  
Protein C ◽  
Activated Protein C ◽  
Epithelial Barrier ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Beneficial Effects ◽  
The Past ◽  
Complex Process

Independent of its well-known anticoagulation effects, activated protein C (APC) exhibits pleiotropic cytoprotective properties. These include anti-inflammatory actions, anti-apoptosis, and endothelial and epithelial barrier stabilisation. Such beneficial effects have made APC an attractive target of research in a plethora of physiological and pathophysiological processes. Of note, the past decade or so has seen the emergence of its roles in cutaneous wound healing—a complex process involving inflammation, proliferation and remodelling. This review will highlight APC’s functions and mechanisms, and detail its pre-clinical and clinical studies on cutaneous wound healing.

scholarly journals Apoptotic bodies derived from mesenchymal stem cells promote cutaneous wound healing via regulating the functions of macrophages

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jin Liu ◽  
Xinyu Qiu ◽  
Yajie Lv ◽  
Chenxi Zheng ◽  
Yan Dong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Mouse Skin ◽  
Skin Wound ◽  
Therapeutic Effects ◽  
Cutaneous Wound Healing ◽  
Apoptotic Bodies ◽  
Skin Wound Healing ◽  
Cutaneous Wound

Abstract Background As the major interface between the body and the external environment, the skin is liable to various injuries. Skin injuries often lead to severe disability, and the exploration of promising therapeutic strategies is of great importance. Exogenous mesenchymal stem cell (MSC)-based therapy is a potential strategy due to the apparent therapeutic effects, while the underlying mechanism is still elusive. Interestingly, we observed the extensive apoptosis of exogenous bone marrow mesenchymal stem cells (BMMSCs) in a short time after transplantation in mouse skin wound healing models. Considering the roles of extracellular vesicles (EVs) in intercellular communication, we hypothesized that the numerous apoptotic bodies (ABs) released during apoptosis may partially contribute to the therapeutic effects. Methods ABs derived from MSCs were extracted, characterized, and applied in mouse skin wound healing models, and the therapeutic effects were evaluated. Then, the target cells of ABs were explored, and the effects of ABs on macrophages were investigated in vitro. Results We found ABs derived from MSCs promoted cutaneous wound healing via triggering the polarization of macrophages towards M2 phenotype. In addition, the functional converted macrophages further enhanced the migration and proliferation abilities of fibroblasts, which together facilitated the wound healing process. Conclusions Collectively, our study demonstrated that transplanted MSCs promoted cutaneous wound healing partially through releasing apoptotic bodies which could convert the macrophages towards an anti-inflammatory phenotype that plays a crucial role in the tissue repair process.

scholarly journals Cutaneous Wound Healing in Diabetic Mice Is Improved by Topical Mineralocorticoid Receptor Blockade

2020 ◽  
Vol 140 (1) ◽  
pp. 223-234.e7 ◽  
Author(s):  
Van Tuan Nguyen ◽  
Nicolette Farman ◽  
Roberto Palacios-Ramirez ◽  
Maria Sbeih ◽  
Francine Behar-Cohen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mineralocorticoid Receptor ◽  
Receptor Blockade ◽  
Cutaneous Wound Healing ◽  
Diabetic Mice ◽  
Cutaneous Wound ◽  
Mineralocorticoid Receptor Blockade

scholarly journals MSC-Derived Exosome Promotes M2 Polarization and Enhances Cutaneous Wound Healing

2019 ◽  
Vol 2019 ◽  
pp. 1-16 ◽  
Author(s):  
Xiaoning He ◽  
Zhiwei Dong ◽  
Yina Cao ◽  
Han Wang ◽  
Shiyu Liu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Wound Repair ◽  
Macrophage Polarization ◽  
Therapeutic Effects ◽  
Cutaneous Wound Healing ◽  
Bone Marrow Mscs ◽  
Wound Site ◽  
Cutaneous Wound ◽  
M2 Polarization

Mesenchymal stem cell transplantation (MSCT) promotes cutaneous wound healing. Numerous studies have shown that the therapeutic effects of MSCT appear to be mediated by paracrine signaling. However, the cell-cell interaction during MSCT between MSCs and macrophages in the region of cutaneous wound healing is still unknown. In this study, early depletion of macrophages delayed the wound repair with MSC injection, which suggested that MSC-mediated wound healing required macrophages. Moreover, we demonstrated that systemically infused bone marrow MSCs (BMMSCs) and jaw bone marrow MSCs (JMMSCs) could translocate to the wound site, promote macrophages toward M2 polarization, and enhance wound healing.In vitrococulture of MSCs with macrophages enhanced their M2 polarization. Mechanistically, we found that exosomes derived from MSCs induced macrophage polarization and depletion of exosomes of MSCs reduced the M2 phenotype of macrophages. Infusing MSCs without exosomes led to lower number of M2 macrophages at the wound site along with delayed wound repair. We further showed that the miR-223, derived from exosomes of MSCs, regulated macrophage polarization by targeting pknox1. These findings provided the evidence that MSCT elicits M2 polarization of macrophages and may accelerate wound healing by transferring exosome-derived microRNA.

scholarly journals Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing

2021 ◽  
Vol 9 ◽  
Author(s):  
Melanie Pichlsberger ◽  
Urška Dragin Jerman ◽  
Hristina Obradović ◽  
Larisa Tratnjek ◽  
Ana Sofia Macedo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Animal Models ◽  
Cutaneous Wound Healing ◽  
Isolated Cells ◽  
Cutaneous Wound ◽  
Beneficial Effects ◽  
Preclinical Animal Models ◽  
Large Animals ◽  
The Impact

Knowledge of the beneficial effects of perinatal derivatives (PnD) in wound healing goes back to the early 1900s when the human fetal amniotic membrane served as a biological dressing to treat burns and skin ulcerations. Since the twenty-first century, isolated cells from perinatal tissues and their secretomes have gained increasing scientific interest, as they can be obtained non-invasively, have anti-inflammatory, anti-cancer, and anti-fibrotic characteristics, and are immunologically tolerated in vivo. Many studies that apply PnD in pre-clinical cutaneous wound healing models show large variations in the choice of the animal species (e.g., large animals, rodents), the choice of diabetic or non-diabetic animals, the type of injury (full-thickness wounds, burns, radiation-induced wounds, skin flaps), the source and type of PnD (placenta, umbilical cord, fetal membranes, cells, secretomes, tissue extracts), the method of administration (topical application, intradermal/subcutaneous injection, intravenous or intraperitoneal injection, subcutaneous implantation), and the type of delivery systems (e.g., hydrogels, synthetic or natural biomaterials as carriers for transplanted cells, extracts or secretomes). This review provides a comprehensive and integrative overview of the application of PnD in wound healing to assess its efficacy in preclinical animal models. We highlight the advantages and limitations of the most commonly used animal models and evaluate the impact of the type of PnD, the route of administration, and the dose of cells/secretome application in correlation with the wound healing outcome. This review is a collaborative effort from the COST SPRINT Action (CA17116), which broadly aims at approaching consensus for different aspects of PnD research, such as providing inputs for future standards for the preclinical application of PnD in wound healing.

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