scholarly journals Effect of Lactobacillus plantarum TWK10 on Exercise Physiological Adaptation, Performance, and Body Composition in Healthy Humans

Nutrients ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2836 ◽  
Author(s):  
Wen-Ching Huang ◽  
Mon-Chien Lee ◽  
Chia-Chia Lee ◽  
Ker-Sin Ng ◽  
Yi-Ju Hsu ◽  
...  
Keyword(s):  
Body Composition ◽  
Lactobacillus Plantarum ◽  
Athletic Training ◽  
Exercise Performance ◽  
Exercise Physiology ◽  
Physiological Adaptation ◽  
High Dose ◽  
Dependent Manner ◽  
Double Blind ◽  
Healthy Humans

Probiotics have been rapidly developed for health promotion, but clinical validation of the effects on exercise physiology has been limited. In a previous study, Lactobacillus plantarum TWK10 (TWK10), isolated from Taiwanese pickled cabbage as a probiotic, was demonstrated to improve exercise performance in an animal model. Thus, in the current study, we attempted to further validate the physiological function and benefits through clinical trials for the purpose of translational research. The study was designed as a double-blind placebo-controlled experiment. A total of 54 healthy participants (27 men and 27 women) aged 20–30 years without professional athletic training were enrolled and randomly allocated to the placebo, low (3 × 1010 colony forming units (CFU)), and high dose (9 × 1010 CFU) TWK10 administration groups (n = 18 per group, with equal sexes). The functional and physiological assessments were conducted by exhaustive treadmill exercise measurements (85% VO2max), and related biochemical indices were measured before and after six weeks of administration. Fatigue-associated indices, including lactic acid, blood ammonia, blood glucose, and creatinine kinase, were continuously monitored during 30 min of exercise and a 90 min rest period using fixed intensity exercise challenges (60% VO2max) to understand the physiological adaptation. The systemic inflammation and body compositions were also acquired and analyzed during the experimental process. The results showed that TWK10 significantly elevated the exercise performance in a dose-dependent manner and improved the fatigue-associated features correlated with better physiological adaptation. The change in body composition shifted in the healthy direction for TWK10 administration groups, especially for the high TWK10 dose group, which showed that body fat significantly decreased and muscle mass significantly increased. Taken together, our results suggest that TWK10 has the potential to be an ergogenic aid to improve aerobic endurance performance via physiological adaptation effects.

scholarly journals A Single Administration of AZP-3601, a Novel, Long-Acting PTH Analog, Induces a Significant and Sustained Calcemic Response: Preliminary Data From a Randomized, Double-Blind, Placebo-Controlled Phase 1 Study

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A254-A254
Author(s):  
Soraya Allas ◽  
Michel Ovize ◽  
Michael D Culler ◽  
Clarisse Geraul ◽  
Jeroen van de Wetering ◽  
...  
Keyword(s):  
Serum Calcium ◽  
Clinical Symptoms ◽  
Phase 1 ◽  
High Dose ◽  
Single Administration ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Healthy Humans ◽  
Dose Dependent

Abstract Hypoparathyroidism is a rare disease characterized by a deficiency in parathyroid hormone (PTH) that results in hypocalcemia and hyperphosphatemia. Current treatment approaches, including high dose oral calcium and active vitamin D, as well as recombinant human PTH (1–84), do not provide adequate or consistent control of either serum calcium or clinical symptoms over a full 24-hour period. AZP-3601 is a novel 36 amino-acid PTH analog that has been designed to potently bind to the R0 conformation of the PTH1 receptor, which results in prolonged signaling responses in vitro and prolonged calcemic responses in animals despite having a short circulating half-life. A Phase 1 double-blind, placebo-controlled, single and multiple ascending dose study is being conducted to evaluate the safety, tolerability and pharmacodynamics of AZP-3601 in healthy adults. Here we report data from the first cohorts of the single ascending dose portion of the study. Sequential cohorts of 4 (cohort 1) to 8 (cohort 2 to 4) healthy male subjects aged 18–60 years, with a body mass index of 19–28 kg/m2, were assigned to receive 5, 10, 20 or 40μg of AZP-3601 or placebo at a ratio of 3:1. The study drug was administered in the morning by subcutaneous injection in the abdominal wall and was well tolerated with no remarkable adverse events. As compared with placebo controls, AZP-3601 treatment produced a clear, dose-dependent increase in mean albumin-adjusted serum calcium values from baseline. The normal physiological diurnal variation of albumin-adjusted serum calcium was gradually attenuated with 5 and 10μg AZP-3601, and was completely eliminated with 20μg. With the dose of 40μg AZP-3601, mean albumin-adjusted serum calcium values were significantly increased but stayed within normal laboratory range and remained elevated through at least 24 hours post-administration. We observed a dose-dependent decrease in mean endogenous serum PTH that was significantly correlated with the concomitant increase in mean serum calcium. These data provide initial evidence of the pharmacodynamic effect of AZP-3601 in healthy humans characterized by a sustained calcemic response for at least 24 hours following a single administration.

scholarly journals Comparison of Serum Choline and Trimethylamine N-oxide after ingestion of Alpha Glyceryl Phosphoryl Choline and Choline Salts.

2021 ◽  
Vol 02 ◽  
Author(s):  
Greggory R. Davis ◽  
David Bellar ◽  
Randy L. Aldret
Keyword(s):  
Health Outcomes ◽  
Dietary Supplement ◽  
Exercise Performance ◽  
Low Dose ◽  
Choline Chloride ◽  
Serum Levels ◽  
High Dose ◽  
Concomitant Increase ◽  
Double Blind ◽  
Phosphoryl Choline

Background: Choline supplements may provide potential improvements to health outcomes and exercise performance, yet the bioavailability of choline supplements is poorly understood. Objective: The objective of the present investigation was to examine the levels of serum Choline and Trimethylamine N-oxide (TMAO) after two dose of Alpha Glyceryl Phosphoryl Choline (A-GPC) in comparison to common choline salts. Methods: High-dose and low-dose A-GPC along with choline salt supplements were administered to participants over the course of four weeks in a randomized double-blind fashion. Serum levels of choline and TMAO, the gut byproduct of choline were examined acutely over the course of four hours and again following one week and four weeks of supplementation. Results: High-dose A-GPC and the choline chloride supplement yielded significantly higher choline levels compared to low-dose A-GPC and choline bitartate (F=31.31, p<0.01) though the effect for time was not significant. TMAO levels were not significantly different between supplements (F=1.96. p=0.1361) or across time (F=0.0795, p=0.7795). Conclusion: A-GPC increases serum choline similar to that seen with high-dose choline chloride ingestion without a concomitant increase in TMAO levels and therefore, may be a desirable option as a dietary supplement.

scholarly journals High dose saccharin supplementation does not induce gut microbiota dysbiosis or glucose intolerance in healthy humans and mice

2020 ◽  
Author(s):  
Joan Serrano ◽  
Kathleen R Smith ◽  
Audra L Crouch ◽  
Vandana Sharma ◽  
Fanchao Yi ◽  
...  
Keyword(s):  
Glucose Tolerance ◽  
Gut Microbiota ◽  
Glucose Intolerance ◽  
Ex Vivo ◽  
Daily Intake ◽  
Sweet Taste ◽  
High Dose ◽  
Double Blind ◽  
Genetic Ablation ◽  
Healthy Humans

Abstract Background : Non-caloric artificial sweeteners (NCAS) are widely used as a substitute for dietary sugars to control body weight or glycemia. Paradoxically, saccharin and other NCAS have been reported to induce glucose intolerance in mice fed a high-fat diet and in a subset of humans by directly inducing unfavorable changes in gut microbiota. These findings have raised concerns about NCAS and called into question their broad use. Whether these results can be generalized to healthy populations consuming conventional diets is unknown. It is also unclear how different NCAS, that do not share a common chemical structure, can produce identical direct effects on gut microbiota. A common feature of all NCAS is their strong affinity for sweet taste receptors (STRs) which are expressed in the intestine. However, their role in mediating NCAS-induced effects has not been addressed. Results : We conducted a double-blind, placebo-controlled, parallel arm study exploring the effects of saccharin on gut microbiota and glucose tolerance in healthy men and women. Participants were randomized to placebo, saccharin, lactisole (STR inhibitor), or saccharin with lactisole administered in capsules twice daily to achieve the maximum acceptable daily intake for two weeks. In parallel, we performed a ten-week study administering high-dose saccharin in the drinking water of chow-fed mice with genetic ablation of STRs (T1R2-KO) and wild-type (WT) littermate controls. In humans and mice alike, none of the interventions affected glucose or hormonal responses to a glucose tolerance test, nor ex vivo glucose absorption in mice. Similarly, saccharin supplementation did not alter microbial diversity or abundance at any taxonomic level in humans or mice. No treatment effects were also noted in readouts of microbial activity such as fecal metabolites or short chain fatty acids (SCFA). However, compared to WT, T1R2-KO mice were protected from age-dependent increases in fecal SCFA and the development of glucose intolerance.

scholarly journals Pharmacokinetic Profile of Spectrum Reduced Nicotine Cigarettes

2019 ◽  
Vol 22 (2) ◽  
pp. 273-279 ◽  
Author(s):  
Helen M Kamens ◽  
Constanza P Silva ◽  
Russell T Nye ◽  
Carley N Miller ◽  
Nayantara Singh ◽  
...  
Keyword(s):  
Subjective Effects ◽  
Pharmacokinetic Profile ◽  
High Dose ◽  
Dependent Manner ◽  
Double Blind ◽  
Nicotine Content ◽  
Nicotine Concentration ◽  
Before And After ◽  
Double Blind Design ◽  
Dose Dependent

Abstract Introduction Spectrum research cigarettes have been developed with varying nicotine content for use in studies evaluating the effects of a regulatory policy reducing the permissible nicotine content in cigarettes. This study aimed to characterize the nicotine pharmacokinetic profile of Spectrum cigarettes. Methods Twelve daily smokers attended four sessions and had blood nicotine, exhaled carbon monoxide, and subjective effects measured before and after smoking either a single cigarette of their preferred brand or high (10.9 mg/cigarette), medium (3.2 mg/cigarette), or low (0.2 mg/cigarette) nicotine content Spectrum research cigarettes, in a double-blind design with order counterbalanced. Results The boost in blood nicotine concentration was dose-dependent, with a boost of 0.3, 3.9, and 17.3 ng/mL for low-, medium-, and high-nicotine content Spectrum cigarettes. The high dose Spectrum had a similar nicotine boost to the “preferred brand” cigarettes (19 ng/mL). Subjects took longer puffs on the low nicotine cigarettes, but smoked these cigarettes faster than other cigarette types. High nicotine Spectrum cigarettes reduced the urge to smoke more than other cigarette types. Conclusions This study shows that Spectrum research cigarettes produce blood nicotine absorption in a dose-dependent manner, and therefore, are appropriate for use in studies of nicotine reduction in cigarettes. Implications This is the first study to determine the pharmacokinetic profile of Spectrum reduced nicotine content research cigarettes following an overnight abstinence. These data could provide evidence to regulatory agencies about the effects of reduced nicotine cigarettes when considering regulations on tobacco reduction.

scholarly journals Bifidobacterium longum subsp. longum OLP-01 Supplementation during Endurance Running Training Improves Exercise Performance in Middle- and Long-Distance Runners: A Double-Blind Controlled Trial

Nutrients ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 1972
Author(s):  
Che-Li Lin ◽  
Yi-Ju Hsu ◽  
Hsieh-Hsun Ho ◽  
Yung-Cheng Chang ◽  
Yi-Wei Kuo ◽  
...  
Keyword(s):  
Body Composition ◽  
Exercise Performance ◽  
Controlled Trial ◽  
Bifidobacterium Longum ◽  
Animal Experiments ◽  
Walking Distance ◽  
Distance Runners ◽  
Endurance Running ◽  
Long Distance ◽  
Double Blind

Bifidobacterium longum subsp. longum Olympic No. 1 (OLP-01) has been shown in previous animal experiments to improve exercise endurance performance, but this effect has not been confirmed in humans, or more particularly, in athletes. Toward this end, the current study combined OLP-01 supplementation with regular exercise training in well-trained middle- and long-distance runners at the National Taiwan Sport University. The study was designed as a double-blind placebo-controlled experiment. Twenty-one subjects (14 males and seven females aged 20–30 years) were evenly distributed according to total distance (meters) traveled in 12 min to one of the following two groups: a placebo group (seven males and three females) and an OLP-01 (1.5 × 1010 colony forming units (CFU)/day) group (seven males and four females). All the participants received placebo or OLP-01 supplements for five consecutive weeks consisting of three weeks of regular training and two weeks of de-training. Before and after the experiment, the participants were tested for 12-min running/walking distance, and body composition, blood/serum, and fecal samples were analyzed. The results showed that OLP-01 significantly increased the change in the 12-min Cooper’s test running distance and the abundance of gut microbiota. Although no significant change in body composition was found, OLP-01 caused no adverse reactions or harm to the participants’ bodies. In summary, OLP-01 can be used as a sports nutrition supplement, especially for athletes, to improve exercise performance.

scholarly journals Effects of Various Doses of Caffeine Ingestion on Intermittent Exercise Performance and Cognition

2020 ◽  
Vol 10 (9) ◽  
pp. 595 ◽  
Author(s):  
Cuicui Wang ◽  
Yuechuan Zhu ◽  
Cheng Dong ◽  
Zigui Zhou ◽  
Xinyan Zheng
Keyword(s):  
Stroop Task ◽  
Exercise Performance ◽  
Power Output ◽  
Intermittent Exercise ◽  
Peak Power Output ◽  
High Dose ◽  
Double Blind ◽  
Caffeine Ingestion ◽  
Positive Effects ◽  
The Mean

To date, no study has examined the effects of caffeine on prolonged intermittent exercise performance that imitates certain team-sports, and the suitable concentration of caffeine for improved intermittent exercise performance remains elusive. The purpose of the present cross-over, double-blind preliminary study was to investigate effects of low, moderate, and high doses of caffeine ingestion on intermittent exercise performance and cognition. Ten males performed a familiarization session and four experimental trials. Participants ingested capsules of placebo or caffeine (3, 6, or 9 mg/kg) at 1 h before exercise, rested quietly, and then performed cycling for 2 × 30 min. The cycling protocol consisted of maximal power pedaling for 5 s (mass × 0.075 kp) every minute, separated by unloaded pedaling for 25 s and rest for 30 s. At pre-ingestion of capsules, 1 h post-ingestion, and post-exercise, participants completed the Stroop task. The mean power-output (MPO), peak power-output (PPO), and response time (RT) in the Stroop task were measured. Only 3 mg/kg of caffeine had positive effects on the mean PPO and MPO; 3 mg/kg caffeine decreased RTs significantly in the incongruent and congruent conditions. These results indicate that the ingestion of low-dose caffeine had greater positive effects on the participants’ physical strength during prolonged intermittent exercise and cognition than moderate- or high-dose caffeine.

scholarly journals Quercetin with vitamin C and niacin does not affect body mass or composition

2011 ◽  
Vol 36 (3) ◽  
pp. 331-338 ◽  
Author(s):  
Amy M. Knab ◽  
R. Andrew Shanely ◽  
Fuxia Jin ◽  
Melanie D. Austin ◽  
Wei Sha ◽  
...  
Keyword(s):  
Body Composition ◽  
Vitamin C ◽  
Body Mass ◽  
Heterogeneous Group ◽  
P Value ◽  
High Dose ◽  
Normal Body Mass Index ◽  
Double Blind ◽  
Obese Subjects

In vitro and animal data suggest that quercetin affects adipogenesis and basal metabolism; however, whether this metabolic effect translates to reductions in body mass or improvement in body composition in humans is unknown. This study investigated 12-week supplementation of 2 different doses of quercetin, combined with vitamin C and niacin, on body mass and composition in a large, heterogeneous group of adults (n = 941; 60% female, 40% male; 18–85 years of age; 45% normal body mass index, 30% overweight, 25% obese). Subjects were randomized into 3 groups, with supplements administered in double-blind fashion: Q500 = 500 mg quercetin·day–1, Q1000 = 1000 mg quercetin·day–1, and placebo. Quercetin supplements were consumed twice daily over a 12-week period, and pre- and poststudy body mass and composition measurements were taken in an overnight fasted state. A general linear model was used to predict change in body mass and composition across groups with adjustment for demographic and lifestyle factors. Plasma quercetin increased in a dose-responsive manner in both Q500 and Q1000 groups relative to placebo. After adjustment for confounders, no significant differences in body mass (males interaction p value = 0.721, females p = 0.366) or body composition (males p = 0.650, females p = 0.639) were found between Q500 or Q1000 groups compared with placebo. No group differences in body mass or body composition were found in a subgroup of overweight and obese subjects. High-dose quercetin supplementation (500 and 1000 mg·day–1) for 12 weeks in a large, heterogeneous group of adults did not affect body mass or composition.

scholarly journals Lactobacillus plantarum PS128 Improves Physiological Adaptation and Performance in Triathletes through Gut Microbiota Modulation

Nutrients ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 2315 ◽  
Author(s):  
Wen-Ching Huang ◽  
Chun-Hsu Pan ◽  
Chen-Chan Wei ◽  
Hui-Yu Huang
Keyword(s):  
Lactobacillus Plantarum ◽  
Exercise Performance ◽  
Maximal Oxygen Consumption ◽  
Endurance Performance ◽  
Short Chain Fatty Acids ◽  
Physiological Adaptation ◽  
Endurance Running ◽  
Supplement Group ◽  
Significant Difference ◽  
And Performance

A triathlon is an extremely high-intensity exercise and a challenge for physiological adaptation. A triathlete’s microbiome might be modulated by diet, age, medical treatments, lifestyle, and exercise, thereby maintaining aerobiosis and optimum health and performance. Probiotics, prebiotics, and synbiotics have been reported to have health-promoting activities (e.g., immunoregulation and cancer prevention). However, few studies have addressed how probiotics affect the microbiota of athletes and how this translates into functional activities. In our previous study, we found that Lactobacillus plantarum PS128 could ameliorate inflammation and oxidative stress, with improved exercise performance. Thus, here we investigate how the microbiota of triathletes are altered by L. plantarum PS128 supplementation, not only for exercise performance but also for possible physiological adaptation. The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG). Both groups continued with their regular exercise training for the next 4 weeks. The endurance performance, body composition, biochemistries, blood cells, microbiota, and associated metabolites were further investigated. PS128 significantly increased the athletes’ endurance, by about 130% as compared to the PG group, but there was no significant difference in maximal oxygen consumption (VO2max) and composition between groups. The PS128 supplementation (LG) modulated the athlete’s microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG. Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota. In conclusion, PS128 supplementation was associated with an improvement on endurance running performance through microbiota modulation and related metabolites, but not in maximal oxygen uptake.

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