scholarly journals Enzymatic Hydrolysis of a Collagen Hydrolysate Enhances Postprandial Absorption Rate—A Randomized Controlled Trial

Nutrients ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 1064 ◽  
Author(s):  
Kathrine Skov ◽  
Mikkel Oxfeldt ◽  
Rebekka Thøgersen ◽  
Mette Hansen ◽  
Hanne Christine Bertram
Keyword(s):  
Enzymatic Hydrolysis ◽  
Absorption Rate ◽  
Controlled Trial ◽  
Plasma Concentrations ◽  
Collagen Hydrolysate ◽  
Beneficial Effects ◽  
Hydrolyzed Collagen ◽  
Collagen Protein ◽  
Collagen Hydrolysates ◽  
The Impact

Collagen is characterized by its high content of glycine, proline and hydroxyproline, and is found to exert beneficial effects on joint pain related to activity and osteoarthritis. However, to exert any beneficial effects it is essential that collagen is optimally absorbed. This study aimed to investigate the postprandial absorption of collagen and elucidate the impact of an exogenous enzymatic hydrolysis on absorption rate and bioavailability. A randomized, blinded, cross-over study was conducted where ten healthy male subjects received either 35 g enzymatically hydrolyzed collagen protein (EHC), 35 g non-enzymatically hydrolyzed collagen protein (NC) or placebo (250 mL water) on three nonconsecutive days. Blood samples were drawn before, and up to 240 min following, ingestion and the blood metabolome was characterized by nuclear magnetic resonance (NMR)-based metabolomics. A significant increase in the plasma concentration of nearly all amino acids (AAs) was observed over a 240 min period for both EHC and NC. In addition, the absorption rate and bioavailability of glycine, proline and hydroxyproline were significantly higher for EHC (p < 0.05). In conclusion, ingestion of collagen hydrolysates increases postprandial plasma concentrations of AAs over a period of 240 min, and an enzymatic hydrolysis increases the absorption rate and bioavailability of the collagen-rich AAs glycine, proline and hydroxyproline.

scholarly journals Coating of Seeds with Collagen Hydrolysates from Leather Waste

2019 ◽  
Vol 27 (4(136)) ◽  
pp. 59-64 ◽  
Author(s):  
Katarzyna Ławińska ◽  
Magdalena Lasoń-Rydel ◽  
Dorota Gendaszewska ◽  
Edyta Grzesiak ◽  
Katarzyna Sieczyńska ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
Growth And Yield ◽  
Collagen Hydrolysate ◽  
Drought Conditions ◽  
Different Types ◽  
Mineral Additive ◽  
Encapsulation Process ◽  
The Subject ◽  
Collagen Hydrolysates ◽  
The Impact

The subject of this paper is improvement in the growth and yield of three different types of legumes and rape in drought conditions by coating seeds with hydrolysed collagen from tanning waste. In addition, the impact of various additives in the seed shell on the growth of the plant was investigated. The encapsulation process of seeds was conducted on a disc granulator. A centrally placed seed was first coated with a layer of fungicides. The next layer was collagen hydrolysate, collagen hydrolysate with latex or a solution of yellow dextrin and polyvinyl alcohol. The outer layer was a mineral additive e.g. dolomite or kaolin. After the end of the encapsulation process on the disk granulator, all of the seeds tested were sown into soils. Seeds without coating were also sown as control seeds. Seedlings were maintained for 29 days with cultivation without irrigation. The length of the seedlings was analysed for all of the seeds sown. Higher seedling growth values were obtained for seeds coated with collagen hydrolysate in comparison with control seeds (without coating). The use of collagen hydrolysate gave slightly better results than in the case of a solution of dextrin with polyvinyl alcohol.

MO793COMPARATIVE STUDY BETWEEN THE IMPACT OF  ETELCALCETIDE AND CINACALCET ON LEVELS OF IPTH, FGF-23, KLOTHO PROTEIN, SCLEROSTIN, CA AND P IN DIALYSIS PATIENTS  WITH SECONDARY HYPERPARATHYROIDISM. RESULTS FROM A PROSPECTIVE RANDOMIZED TRIAL

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Evgeny Shutov ◽  
Galina Kotlyarova ◽  
Ksenia Lysenko ◽  
Galina Ryabinskaya ◽  
Sergey Lashutin
Keyword(s):  
Alkaline Phosphatase ◽  
Secondary Hyperparathyroidism ◽  
Controlled Trial ◽  
Intravenous Route ◽  
Ipth Level ◽  
Beneficial Effects ◽  
Protein Levels ◽  
Klotho Protein ◽  
Fgf 23 ◽  
The Impact

Abstract Background and Aims The treatment of secondary hyperparathyroidism is one of the main tasks in the correction of mineral and bone disorders (MBD) in patients with chronic kidney disease (CKD). However, the results of therapy for secondary hyperparathyroidism are still unsatisfactory. In our prospective randomized controlled trial were evaluated the effect and safety of 26 weeks of treatment with etelcalcetide (intravenous route of administration) compare with cinacalcet (oral administration) on CKD-MBD parameters in patients on program hemodialysis with secondary hyperparathyroidism. Method The study group included 50 stable patients receiving hemodialysis with secondary hyperparathyroidism (PTH &gt; 300 pg/ml) and corrected Ca level greater than 2.2 mmol/L, who were randomized in a 1: 1 ratio for treatment with etelcalcetide (n = 25) or cinacalcet (n = 25) for 26 weeks. All patients were monthly evaluated the levels of P, Ca, intact parathyroid hormone (iPTH), alkaline phosphatase (ALP); the levels of FGF 23, Klotho protein and sclerostin were assessed once in 3 months. The dose of both drugs was adjusted according to the serum iPTH level. The nature, frequency, and severity of treatment-emergent adverse events were assessed. Results Therapy with cinacalcet and etelcalcetide led to a significant decrease in the level of iPTH in the blood serum from 613.1 ± 235.1 to 302.2 ± 205.1pg/ml (p&lt; 0.01) and from 671.2 ± 272.3 to 358,6 ± 292.5 pg/ml (p &lt;0.01), by 49.2% and 53.4%, respectively. A significant decrease in the levels of corrected Ca was noted in both groups: in the etelcalcetide group from 2.20 ± 0.12 to 2.06 ± 0.18 mmol/L (p &lt;0.05), in the cinacalcet group from 2.25 ± 0.12 to 2.04 ± 0.21 mmol/l (p &lt;0.05). There was no significant change in the P levels. The alkaline phosphatase level significantly decreased in the cinacalcet group from 178.7 ± 116.8 to 78.9 ± 34.1 U/L, p &lt;0.05) and in the etelcalcetide group from 170,3 ± 115.7 to 87.1 ± 30.8 U/L, p &lt;0.05. There was a significant increase in Klotho protein levels by the end of the study from 17.9 ± 5.0 to 57.1 ± 39.3 (p &lt;0.05) and from 17.6 ± 3.7 to 91.6 ± 56.2 pg/ml (p &lt;0.05), respectively, in the cinacalcet and etelcalcetide group. Changes in FGF-23 and sclerostin by 6 months reached statistically significant changes only in the etelcalcetide group, a decrease from the FGF-23 level from 42.7 ± 22.2 to 23.0 ± 12.3 pg/ml and an increase in the level of sclerostin from 1, 59 ± 0.31 to 2.20 ± 0.33 ng/ml (p &lt;0.05). During the study, 2 patients in the cinacalcet group dropped out due to dyspeptic symptoms and 1 patient in the etelcalcetide group dropped out due to hypocalcemia. Conclusion Etelcalcetide and cinacalcet are effective PTH-lowering drugs with a comparable safety profile. Treatment with etelcalcetide, in contrast to cinacalcet, was associated with significant increases in sclerostin and decreases in FGF-23, which may have beneficial effects on outcomes and requires further study.

scholarly journals Impact of Obesity on the Rate of Recurrent Spontaneous Preterm Birth in Women Treated with 17-alpha Hydroxyprogesterone Caproate

2018 ◽  
Vol 35 (09) ◽  
pp. 809-814 ◽  
Author(s):  
Lara Lemon ◽  
Allison Serra ◽  
Shringi Sharma ◽  
Raman Venkataramanan ◽  
Steve Caritis ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Controlled Trial ◽  
Plasma Concentrations ◽  
Pharmacokinetic Studies ◽  
Maternal Body Mass Index ◽  
Omega 3 ◽  
Spontaneous Preterm Birth ◽  
Obesity Class ◽  
Hydroxyprogesterone Caproate ◽  
The Impact

Objective We sought to determine if the rate of recurrent spontaneous preterm birth (PTB) in women treated with 17-α hydroxyprogesterone caproate (17-OHPC) is modified by maternal body mass index (BMI). Study Design We performed a secondary analysis of the Maternal-Fetal Medicine Units Network omega-3 fatty acid supplementation to prevent recurrent PTB randomized controlled trial. All women received 17-OHPC. Results A total of 708 women were included. Rates of spontaneous PTB did not vary significantly by BMI category. With stratification by obesity class and gestational age at delivery, the unadjusted risk for PTB using earlier gestational cutoffs (< 35, 32, and 28 weeks) demonstrated an association between preterm delivery and increasing severity of obesity. With adjustment for potential confounders, there was no statistically significant relationship between BMI and spontaneous PTB. Conclusion We demonstrated that the risk of PTB in women receiving 250 mg 17-OHPC is not dependent on maternal BMI after adjustment for confounding variables. Pharmacokinetic studies have demonstrated a wide variation in plasma concentration of 17-OHPC across the population with likely considerable overlap in plasma concentrations among the obese and nonobese population. Further studies are needed to evaluate the impact of BMI on efficacy of 17-OHPC prior to any dose adjustment in this population.

scholarly journals Effects of Daily Almond Consumption for Six Months on Cognitive Measures in Healthy Older Adults: A Randomized Control Trial

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 1213-1213
Author(s):  
Elizabeth Johnson ◽  
Jelena Mustra Rakic ◽  
Jirayu Tanprasertsuk ◽  
Tammy M Scott ◽  
Helen Rasmussen ◽  
...  
Keyword(s):  
Older Adults ◽  
Working Memory ◽  
Cognitive Function ◽  
Visual Memory ◽  
Controlled Trial ◽  
Serum Markers ◽  
Alpha Tocopherol ◽  
Beneficial Effects ◽  
Funding Sources ◽  
The Impact

Abstract Objectives Cognitive impairment is a major risk factor for the development of dementia. Almonds are rich in nutrients recognized to have beneficial effects on cognitive function. The objective of this study is to determine the impact of almond consumption on cognition in elderly adults. Methods In a 6-mo single-blinded randomized controlled trial the effects of an almond intervention on cognition in healthy middle-aged to older adults were tested. Subjects were assigned to one of three groups: 1.5 oz/d almond (n = 19), 3 oz/d almond (n = 24) or 1.5 oz/d snack mix (matched for macronutrients in 3.0 oz almonds; n = 17). Dietary interviews, serum analyses for tocopherols, magnesium, oxidative status and inflammation biomarkers and cognitive function were assessed at baseline (M0), three (M3) and six (M6) months. Results At M6 serum alpha-tocopherol concentrations were increased by 8% from M0 in the 3 oz almond group (P &lt; 0.05) and no increases were observed in the other groups. Serum markers of oxidative stress and inflammation were not significantly different at M0, M3 and M6 among the three groups. At M6 there were significant improvements in visuospatial working memory, visual memory and learning and spatial planning and working memory in subjects consuming 3 oz/d almonds. The snack mix and 1.5 oz/d almond groups showed no significant changes in these measures. Conclusions The study findings suggest that a long-term intervention with almonds may be an effective dietary strategy for preventing cognitive decline in an older population Funding Sources Almond Board of California, USDA CRIS #8050–51,000-095–02S.

scholarly journals Effects of Extrinsic Wheat Fiber Supplementation on Fecal Weight; A Randomized Controlled Trial

Nutrients ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 298
Author(s):  
Beate Brandl ◽  
Yu-Mi Lee ◽  
Andreas Dunkel ◽  
Thomas Hofmann ◽  
Hans Hauner ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Controlled Trial ◽  
Dry Weight ◽  
Food Products ◽  
Short Chain Fatty Acids ◽  
Normal Diet ◽  
Outcome Variable ◽  
Beneficial Effects ◽  
Wet Weight ◽  
The Impact

Higher fiber intake may confer beneficial effects on health. Our objective was to investigate the impact of 10 g extrinsic wheat fiber on fecal bulk. Therefore, we performed two randomized intervention studies in which we provided extrinsic wheat fiber-enriched products or appropriate control products for five days together with normal diet. In one trial, 10 participants received fiber-enriched food products, whereas in the second study, 19 participants supplemented their daily diet with fiber-enriched drinks. The main outcome variable of this intervention was fecal bulk. Other outcomes were gut microbiota composition, short chain fatty acids in feces, and stool consistency and frequency. Fecal wet weight was significantly increased (p < 0.02) with extrinsic wheat fiber-enriched foods. In contrast, ingestion of extrinsic wheat fiber in the form of drinks did not significantly change fecal wet weight. In both groups, fecal dry weight was not altered upon extrinsic wheat fiber supplementation. However, the intake of fiber-enriched foods resulted in higher acetic acid levels in feces compared to fiber-enriched drinks. Regarding gut microbiota profiles, extrinsic wheat fiber-enriched food products were not associated with substantial alterations. In conclusion, 10 g extrinsic wheat fiber added to a normal diet increased fecal bulk if administered in a solid food matrix, but not if applied in the form of drinks. DRKS, DRKS00015792.Registered 30 October 2018.

scholarly journals Randomized controlled trial of the mySmartSkin web-based intervention to promote skin self-examination and sun protection among individuals diagnosed with melanoma

2021 ◽  
Author(s):  
Sharon L Manne ◽  
Carolyn J Heckman ◽  
Deborah A Kashy ◽  
Lee M Ritterband ◽  
Frances P Thorndike ◽  
...  
Keyword(s):  
Controlled Trial ◽  
Sun Protection ◽  
Web Based ◽  
Participant Engagement ◽  
Beneficial Effects ◽  
Core Components ◽  
Self Examination ◽  
The Impact ◽  
Univariate Analyses

Abstract Adherence to regular, thorough skin self-examination (SSE) and consistent sun protection behaviors among melanoma survivors is relatively low. This study reports on the impact of an online intervention, called mySmartSkin (MSS), on engagement in SSE and sun protection behaviors among melanoma survivors, as well as the mediators of the intervention effects. The intervention was compared with usual care (UC), and primary outcomes were assessed at 24 and 48 weeks. Short-term outcomes were also evaluated at 8 weeks postbaseline. Results demonstrate a significant effect on SSE and sun protection. At all three follow-up assessments, the proportion of participants reporting conducting a thorough SSE in the time since the previous assessment was significantly greater in MSS than in UC. In addition, both multivariate and univariate analyses indicated that engagement in sun protection behaviors was significantly higher in MSS than UC at 24 weeks, but the effect on sun protection at 48 weeks was significant only in multivariate analyses. Beneficial effects of MSS were significantly mediated by knowledge about melanoma and characteristics of suspicious lesions, as well as self-efficacy. Participant engagement in MSS was satisfactory, with approximately two-thirds of participants completing at least two of the three core components. Content was rated as highly trusted, easy to understand, easy to navigate, and helpful. In conclusion, MSS illustrated significant and durable effects on SSE and mixed results on sun protection. Future studies should consider ways to further enhance treatment effects and engagement in MSS.

scholarly journals Evaluation of a Food Supplement with Collagen Hydrolysate and Micronutrients on Skin Appearance and Beauty Effects: A Randomized, Double-Blind, PlaceboControlled Clinical Study with Healthy Subjects

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Modinger Y ◽  
Schon C ◽  
Vogel K ◽  
Brandt M ◽  
Bielfeldt S ◽  
...  
Keyword(s):  
Structural Integrity ◽  
Natural Aging ◽  
Food Supplement ◽  
Collagen Structure ◽  
Collagen Network ◽  
Double Blind ◽  
Collagen Hydrolysate ◽  
Beneficial Effects ◽  
Collagen Peptides ◽  
Hydrolyzed Collagen

Objective: Skin health, skin appearance and skin beauty are influenced by collagen composition of the dermis. Natural aging affects the structural integrity of the collagen network, resulting in drier skin, wrinkle formation and reduced skin elasticity. Orally ingested hydrolyzed collagen reaches the skin tissue and exerts beneficial effects on human skin from within. However, not only collagen peptides, but also micronutrients can beneficially affect skin appearance. Thus, dietary supplements for cosmetic and beauty effects containing combinations of collagen peptides and selected micronutrients are in demand. Aim of this study was to investigate the effect of a food supplement with collagen hydrolysate and micronutrients on skin beauty. The test product was assessed for its capability to reduce wrinkles and to improve skin hydration and collagen structure.

scholarly journals Efficacy of Chondroprotective Food Supplements Based on Collagen Hydrolysate and Compounds Isolated from Marine Organisms

Marine Drugs ◽  
2021 ◽  
Vol 19 (10) ◽  
pp. 542
Author(s):  
Thomas Eckert ◽  
Mahena Jährling-Butkus ◽  
Helen Louton ◽  
Monika Burg-Roderfeld ◽  
Ruiyan Zhang ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Treatment Options ◽  
Clinical Parameter ◽  
Food Supplement ◽  
Marine Organisms ◽  
Early Osteoarthritis ◽  
German Shepherd ◽  
Collagen Hydrolysate ◽  
Beneficial Effects ◽  
Collagen Hydrolysates

Osteoarthritis belongs to the most common joint diseases in humans and animals and shows increased incidence in older patients. The bioactivities of collagen hydrolysates, sulfated glucosamine and a special fatty acid enriched dog-food were tested in a dog patient study of 52 dogs as potential therapeutic treatment options in early osteoarthritis. Biophysical, biochemical, cell biological and molecular modeling methods support that these well-defined substances may act as effective nutraceuticals. Importantly, the applied collagen hydrolysates as well as sulfated glucosamine residues from marine organisms were strongly supported by both an animal model and molecular modeling of intermolecular interactions. Molecular modeling of predicted interaction dynamics was evaluated for the receptor proteins MMP-3 and ADAMTS-5. These proteins play a prominent role in the maintenance of cartilage health as well as innate and adapted immunity. Nutraceutical data were generated in a veterinary clinical study focusing on mobility and agility. Specifically, key clinical parameter (MMP-3 and TIMP-1) were obtained from blood probes of German shepherd dogs with early osteoarthritis symptoms fed with collagen hydrolysates. Collagen hydrolysate, a chondroprotective food supplement was examined by high resolution NMR experiments. Molecular modeling simulations were used to further characterize the interaction potency of collagen fragments and glucosamines with protein receptor structures. Potential beneficial effects of collagen hydrolysates, sulfated glycans (i.e., sulfated glucosamine from crabs and mussels) and lipids, especially, eicosapentaenoic acid (extracted from fish oil) on biochemical and physiological processes are discussed here in the context of human and veterinary medicine.

scholarly journals Trial participants’ experiences of early enhanced speech and language therapy after stroke compared with employed visitor support: a qualitative study nested within a randomized controlled trial

2012 ◽  
Vol 27 (2) ◽  
pp. 174-182 ◽  
Author(s):  
Alys Young ◽  
Timothy Gomersall ◽  
Audrey Bowen ◽  
Keyword(s):  
Randomized Controlled Trial ◽  
Qualitative Study ◽  
Controlled Trial ◽  
Speech And Language ◽  
Language Therapy ◽  
Speech And Language Therapy ◽  
Beneficial Effects ◽  
Randomized Controlled ◽  
The Impact ◽  
Trial Participants

Objectives: To explore trial participants’ experiences of the process and outcomes of early, enhanced speech and language therapy after stroke with support from an employed visitor. Design: Qualitative study nested within a randomized controlled trial. Participants: Twney-two people who, after stroke, had a diagnosis of aphasia (12), dysarthria (5) or both (5) and who participated in the ACT NoW study. Setting: Eight English NHS usual care settings. Method: Individual interviews. Thematic content analysis assisted by a bespoke data transformation protocol for incorporating non-verbal and semantically ambiguous data. Results: Participants highly regarded regular and sustained contact with someone outside of immediate family/friends who engaged them in deliberate activities/communication in the early months after stroke. Participants identified differences in the process of intervention between speech and language therapists and employed visitors. But no major discriminations were made between the impact or value of this contact according to whether provided by a speech and language therapist or employed visitor. Participant-defined criteria for effectiveness of contact included: impact on mood and confidence, self-recognition of progress and the meeting of individual needs. Conclusions: As in the randomized controlled trial, participants reported no evidence of added benefit of early communication therapy beyond that from attention control. The findings do not imply that regular contact with any non-professional can have beneficial effects for someone with aphasia or dysarthria in the early weeks following a stroke. The study points to specific conditions that would have to be met for contact to have a positive effect.

scholarly journals Impact of Preventive and Therapeutic Zinc Supplements on Citrulline Concentration and the kynurenine: tryptophan Ratio Among Lao Children: A Randomized Controlled Trial (OR07-02-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
K Ryan Wessells ◽  
Guy-Marino Hinnouho ◽  
Maxwell Barffour ◽  
Somphou Sayasone ◽  
Charles Arnold ◽  
...  
Keyword(s):  
Linear Growth ◽  
Controlled Trial ◽  
Plasma Concentrations ◽  
Intestinal Function ◽  
Linear Regression Models ◽  
Double Blind ◽  
Randomized Controlled ◽  
Zinc Supplements ◽  
The Impact ◽  
Parent Trial

Abstract Objectives To investigate the impact of different forms of zinc supplementation on plasma citrulline (CIT), kynurenine (KYN) and tryptophan (TRP) concentrations and the kynurenine: tryptophan ratio (KTR), considered as markers of intestinal function and systemic inflammatory response, among young Lao children. Methods In a randomized controlled double-blind trial, 3407 children aged 6–23 mo were randomized into one of four groups and followed for ∼36 weeks: daily preventive zinc dispersible tablet (7 mg zinc; PZ), daily multiple micronutrient powder (10 mg zinc, 6 mg iron and 13 other micronutrients; MNP), therapeutic zinc supplements for the treatment of diarrhea (20 mg/d for 10 days with each diarrhea episode; TZ), or daily placebo powder (Control). Plasma samples at baseline and endline for 359 children participating in the parent trial were analyzed at the NIH West Coast Metabolomics Center (UC Davis); plasma CIT, KYN and TRP concentrations were determined by hydrophilic interaction chromatography (HILIC) quadrupole time of flight mass spectrometer (QTOF) tandem mass spectrometry (MS/MS). Linear regression models were used to assess the treatment effect, controlling for baseline value, child age and district. Results The parent trial found no overall group-wise effects on linear growth or diarrhea outcomes. In the subgroup included in the present analyses, mean age at enrollment was 16.0 ± 4.9 mo, 37% were stunted and 83% were zinc deficient. At baseline, mean plasma CIT, KYN and TRP concentrations were 24.6 ± 5.4 µM, 3.27 ± 0.83 µM and 72.3 ± 12.9 µM, respectively; the mean KT ratio was 0.046 ± 0.013. 5% of children had low CIT (< 17 µM) and no children had low TRP (< 35 µM). At endline, there were no differences among intervention groups in mean plasma CIT (25.0–26.6 µM, P = 0.287), KYN (2.96–3.11 µM, P = 0.115), TRP (66.1–70.0 µM, P = 0.151) or the KTR (0.046–0.047, P = 0.981). Conclusions In this population, PZ, MNP and TZ had no overall effect on plasma concentrations of CIT, KYN, TRP and the KTR. We plan to further explore if these markers of intestinal function were predictive of subsequent linear growth, or modified the growth response to supplementation. Funding Sources The Bill & Melinda Gates Foundation, Nutrition International and the Mathile Institute for the Advancement of Human Nutrition.

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