scholarly journals Miso (Fermented Soybean Paste) Suppresses Visceral Fat Accumulation in Mice, Especially in Combination with Exercise

Nutrients ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 560 ◽  
Author(s):  
Ran Okouchi ◽  
Yuto Sakanoi ◽  
Tsuyoshi Tsuduki
Keyword(s):  
Adipose Tissue ◽  
White Adipose Tissue ◽  
High Fat Diet ◽  
Exercise Group ◽  
Peroxisome Proliferator ◽  
High Fat ◽  
Tissue Weight ◽  
Adipose Tissue Weight ◽  
Soybean Paste ◽  
Freeze Dried

We investigated whether the difference in miso consumption between the Japanese diets of 1975 and 2010 has influenced the observed increase in diet-induced obesity. To recreate the 2010 and 1975 Japanese high-fat diets with the corresponding proportions of miso, freeze-dried miso was added to high-fat mouse feed at 1.6% and 2.6%, respectively. When 5-week-old male Institute of Cancer Research (ICR) mice were provided each of these diets ad libitum for 8 weeks, it was found that the white adipose tissue weight and adipocyte area were lower in mice receiving the 1975 diet than in those receiving the 2010 diet. Therefore, high miso consumption is one reason why the 1975 Japanese diet tended to not lead to obesity. Next, the combined effects of treadmill exercise and miso consumption were investigated. The mice were divided into three groups, which were provided either a high-fat diet (group C), a high-fat diet with exercise (group C + E), or a miso-supplemented high-fat diet with exercise (group M + E) for 8 weeks. In this experiment, the white adipose tissue weight and adipocyte area in group M + E were lower than in group C. When the mRNA expression of lipid metabolism-associated genes in adipose tissue was measured, we found that expression of Hsl (lipase, hormone sensitive), which is involved in lipolysis, and Pparγ (peroxisome proliferator activated receptor gamma), which regulates adipocyte differentiation upstream of Hsl, was increased in group M + E. These results clearly demonstrated that lipid accumulation in the adipose tissues is suppressed by miso consumption in combination with exercise.

scholarly journals Allium hookeri Root Extract Inhibits Adipogenesis by Promoting Lipolysis in High Fat Diet-Induced Obese Mice

Nutrients ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 2262 ◽  
Author(s):  
Kim ◽  
Jang ◽  
Lee
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
White Adipose Tissue ◽  
High Fat Diet ◽  
Obese Mice ◽  
High Fat ◽  
Tissue Weight ◽  
Adipose Tissue Weight ◽  
Allium Hookeri

: Allium hookeri (AH) is widely consumed as a herbal medicine. It possesses biological activity against metabolic diseases. The objective of this study was to investigate effects of AH root water extract (AHR) on adipogenesis in 3T3-L1 cells and in high-fat diet (HFD)-induced obese mice. AHR inhibited lipid accumulation during adipocyte differentiation by downregulation of gene expression, such as hormone sensitive lipase (HSL), lipoprotein lipase (LPL) and an adipogenic gene, CCAAT/enhancer binding protein-α in 3T3-L1 preadipocytes. Oral administration of AHR significantly suppressed body weight gain, adipose tissue weight, serum leptin levels, and adipocyte cell size in HFD-induced obese mice. Moreover, AHR significantly decreased hepatic mRNA expression levels of cholesterol synthesis genes, such as 3-hydroxy-3-methylglutaryl CoA reductase, sterol regulatory element-binding transcription factor (SREBP)-2, and low-density lipoprotein receptor, as well as fatty acid synthesis genes, such as SREBP-1c and fatty acid synthase. Serum triglyceride levels were also lowered by AHR, likely as a result of the upregulating gene involved in fatty acid β-oxidation, carnitine palmitoyltransferase 1a, in the liver. AHR treatment activated gene expression of peroxisome proliferator-activated receptor-γ, which might have promoted HSL and LPL-medicated lipolysis, thereby reducing white adipose tissue weight. In conclusion, AHR treatment can improve metabolic alterations induced by HFD in mice by modifying expression levels of genes involved in adipogenesis, lipogenesis, and lipolysis in the white adipose tissue and liver.

scholarly journals Anti-Obesity and Hypocholesterolemic Actions of Protamine-Derived Peptide RPR (Arg-Pro-Arg) and Protamine in High-Fat Diet-Induced C57BL/6J Mice

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2501
Author(s):  
Maihemuti Mijiti ◽  
Ryosuke Mori ◽  
Bingyu Huang ◽  
Kenichiro Tsukamoto ◽  
Keisuke Kiriyama ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
High Fat Diet ◽  
Fecal Excretion ◽  
Control Group ◽  
High Fat ◽  
Tissue Weight ◽  
Cholesterol Levels ◽  
Adipose Tissue Weight ◽  
Fas Mrna ◽  
Hypocholesterolemic Peptide

Dietary protamine can ameliorate hyperlipidemia; however, the protamine-derived active peptide and its hypolipidemic mechanism of action are unclear. Here, we report the discovery of a novel anti-obesity and hypocholesterolemic peptide, RPR (Arg-Pro-Arg), derived from protamine in mice fed a high-fat diet for 50 days. Serum cholesterol levels were significantly lower in the protamine and RPR groups than in the control group. White adipose tissue weight was significantly decreased in the protamine and RPR groups. The fecal excretion of cholesterol and bile acid was significantly higher in the protamine and RPR groups than in the control group. We also observed a significant decrease in the expression of hepatic SCD1, SREBP1, and adipocyte FAS mRNA, and significantly increased expression of hepatic PPARα and adipocyte PPARγ1 mRNA in the protamine group. These findings demonstrate that the anti-obesity effects of protamine are linked to the upregulation of adipocyte PPARγ1 and hepatic PPARα and the downregulation of hepatic SCD1 via SREBP1 and adipocyte FAS. RPR derived from protamine has a crucial role in the anti-obesity action of protamine by evaluating the effective dose of adipose tissue weight loss.

Burdock Fermented byAspergillus awamoriElevates CecalBifidobacterium, and Reduces Fecal Deoxycholic Acid and Adipose Tissue Weight in Rats Fed a High-Fat Diet

2013 ◽  
Vol 77 (1) ◽  
pp. 53-57 ◽  
Author(s):  
Yukako OKAZAKI ◽  
Novita Vivi SITANGGANG ◽  
Satoko SATO ◽  
Nanae OHNISHI ◽  
Junji INOUE ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
High Fat Diet ◽  
Deoxycholic Acid ◽  
High Fat ◽  
Tissue Weight ◽  
Adipose Tissue Weight

Stimulatory Effects of Extracts Prepared from Salix babylonica L. on Fat Catabolism in Mice Fed High-Fat Diet

2012 ◽  
Vol 518-523 ◽  
pp. 498-501
Author(s):  
Ke Yue Liu
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
High Fat Diet ◽  
Lipid Emulsion ◽  
High Fat ◽  
Tissue Weight ◽  
Adipose Tissue Weight ◽  
Ethanol Extracts ◽  
Salix Babylonica

Effects of the ethanol extracts prepared from Salix babylonica L. leaves on the fat deposit induced in mice by feeding a high-fat-diet for 9 wks were studied. Increase in body weight and parametrial adipose tissue weight containing 2,5 or 10g (extract) /kg food was suppressed as compared to that observed in mice fed the high-fat-diet alone. Futhermore, the enthanol extract inhibited the elevation of blood triacylglycerol in rats administered orally a lipid emulsion as compared to that in rats given the emulsion alone. Experiments are now undergoing to isolate other ingredients from the extract and test them for anti-obesity effects.

scholarly journals Antidiabetic Effects ofCarassius auratusComplex Formula in High Fat Diet Combined Streptozotocin-Induced Diabetic Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Zhi-Hong Wang ◽  
Cheng-Chin Hsu ◽  
Hui-Hsuan Lin ◽  
Jing-Hsien Chen
Keyword(s):  
Adipose Tissue ◽  
Blood Glucose ◽  
Glucose Tolerance ◽  
High Fat Diet ◽  
Carassius Auratus ◽  
Diabetic Mice ◽  
High Fat ◽  
Tissue Weight ◽  
Adipose Tissue Weight ◽  
Antidiabetic Effects

Carassius auratuscomplex formula, includingCarassius auratus, Rhizoma dioscoreae,Lycium chinense, andRehmannia glutinosaLibosch, is a combination prescription of traditional Chinese medicine, which has always been used to treat diabetes mellitus in ancient China. In this study, we provided experimental evidence for the use ofCarassius auratuscomplex formula in the treatment of high fat diet combined streptozotocin- (STZ-) induced type 2 diabetes.Carassius auratuscomplex formula aqueous extract was prepared and the effects of it on blood glucose, serum insulin, adipose tissue weight, oral glucose tolerance test (OGTT), total cholesterol, and triglyceride (TG) levels in mice were measured. Moreover, adiponectin, TG synthesis related gene expressions, and the inhibitory effect of aldose reductase (AR) were performed to evaluate its antidiabetic effects. After the 8-week treatment, blood glucose, insulin levels, and adipose tissue weight were significantly decreased. OGTT and HOMA-IR index showed improved glucose tolerance. It could also lower plasma TG, TC, and liver TG levels. Furthermore,Carassius auratuscomplex formula could inhibit the activity of AR and restore adiponectin expression in serum. Based on these findings, it is suggested thatCarassius auratuscomplex formula possesses potent anti-diabetic effects on high fat diet combined STZ-induced diabetic mice.

scholarly journals Effective Suppression of Adipose Tissue Weight Gain in High-fat Diet-fed Mice by 'Benifuuki' Green Tea Consumption

2009 ◽  
Vol 56 (7) ◽  
pp. 403-411 ◽  
Author(s):  
Hiroyuki Inagaki ◽  
Masanori Sugitani ◽  
Yuko Setoguchi ◽  
Ryouichi Ito ◽  
Yukihiro Oritani ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Weight Gain ◽  
Green Tea ◽  
High Fat Diet ◽  
Tea Consumption ◽  
High Fat ◽  
Tissue Weight ◽  
Adipose Tissue Weight ◽  
Effective Suppression

Seasonal obesity in Syrian hamsters: effects of age, diet, photoperiod, and melatonin

1984 ◽  
Vol 247 (2) ◽  
pp. R328-R334 ◽  
Author(s):  
G. N. Wade ◽  
T. J. Bartness
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
High Fat Diet ◽  
Reproductive Function ◽  
Fat Content ◽  
High Fat ◽  
Melatonin Treatment ◽  
Tissue Weight ◽  
Adipose Tissue Weight ◽  
Brown Adipose

Two experiments examined the effects of photoperiod, melatonin, and diet on body weight in female Syrian hamsters (Mesocricetus auratus). In experiment 1, daily injections of 25 micrograms melatonin increased body weight when given 3 h before lights-out but not when given at the midpoint of the light phase, in a 16-h light-8-h dark cycle (LD 16:8). Ten micrograms of melatonin, given 3 h before lights-out, were sufficient to increase body weight and fat content, to increase interscapular brown adipose tissue weight, to decrease uterine weight, and to interrupt estrous cyclicity. However, 2.5 micrograms of melatonin increased body weight and fat content without affecting brown adipose tissue weight or reproductive function. In experiment 2, melatonin treatment, exposure to a short photoperiod (LD 8:16), and feeding a high-fat diet increased body weight gain in weanling (25-day-old) female hamsters. When melatonin treatment or high-fat diet were withdrawn, hamsters reduced their food intake, and their body weight and fat content returned to control levels. After 15-17 wk in the short photoperiod, hamsters also began to undereat, and their body weight and fat content returned to control levels. These findings suggest several conclusions. 1) As with the changes in reproductive function, melatonin is effective at increasing body weight only when given at certain times of day. 2) Not all end points are equally responsive to melatonin, suggesting that they are independent of one another. 3) Weanling hamsters respond to photoperiod, melatonin, and diet just as adults do. 4) The striking obesities induced by these manipulations are completely reversible.

scholarly journals Octacosanol affects lipid metabolism in rats fed on a high-fat diet

1995 ◽  
Vol 73 (3) ◽  
pp. 433-441 ◽  
Author(s):  
S. Kato ◽  
K.-I. Karino ◽  
S. Hasegawa ◽  
J. Nagasawa ◽  
A. Nagasaki ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipid Metabolism ◽  
High Fat Diet ◽  
Hormone Sensitive Lipase ◽  
Lipid Absorption ◽  
Epididymal Adipose Tissue ◽  
High Fat ◽  
Perirenal Adipose Tissue ◽  
Tissue Weight ◽  
Adipose Tissue Weight

The effect of dietary octacosanol, a long-chain alcohol, on lipid metabolism was investigated in rats fed on a high-fat diet for 20 d. The addition of octacosanol (10 g/kg diet) to the high-fat diet led to a significant reduction (P < 0·05) in the perirenal adipose tissue weight without decrease of the cell number, suggesting that octacosanol may suppress lipid accumulation in this tissue, whereas no effect was seen in the epididymal adipose tissue weight and in the lipid content in liver. Octacosanol supplementation decreased the serum triacylglycerol concentration, and enhanced the concentration of serum fatty acids, probably through inhibition of hepatic phosphatidate phosphohydrolase (EC 3·1·3·4). Though the activity of hormone-sensitive lipase (EC 3·1·1·3) was not influenced by octacosanol, higher activities of lipoprotein lipase (EC 3·1·1·34) in the perirenal adipose tissue and the total oxidation rate of fatty acid in muscle were observed. Lipid absorption was not affected by the inclusion of octacosanol. Thus, the present results suggest that the dietary incorporation of octacosanol into a high-fat diet affects some aspects of lipid metabolism.

scholarly journals A Standardized Aframomum melegueta Seed Extract Regulates Browning of White Adipose Tissue in High-Fat Diet Model Mice

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110337
Author(s):  
Heggar Venkataramana Sudeep ◽  
Illuri Ramanaiah ◽  
Raj Amritha ◽  
Puttaswamy Naveen ◽  
Kuluvar Gouthamchandra ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
White Adipose Tissue ◽  
High Fat Diet ◽  
Seed Extract ◽  
Peroxisome Proliferator ◽  
High Fat ◽  
Peroxisome Proliferator Activated Receptor ◽  
Bat Activity ◽  
Brown Adipose ◽  
Beige Adipocytes

Thermogenesis-mediated energy expenditure is a promising strategy to combat obesity. Aframomum melegueta commonly known as grains of paradise (GP) is a popular spice with medicinal attributes in promoting health. We have demonstrated the thermogenic effects of a standardized A melegueta seed extract (AMSE) containing not <10% 6-paradol in high fat diet-fed (HFD) mice. The 6-week oral ingestion of 20 and 40 mg/kg AMSE significantly limited the weight gain, improved the brown adipose tissue (BAT) activity in HFD mice. Interestingly, AMSE markedly induced the beige adipocytes in epididymal white adipose tissue (eWAT). AMSE treatment led to the upregulation of marker proteins i.e., uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor-gamma-coactivator 1-alpha (PGC-1α), and peroxisome proliferator-activated receptor gamma (PPARγ) in eWAT and BAT. Our findings add to the current understanding of the thermogenic potentials of GP seed extract and report that the extract can stimulate the browning of WATs in addition to enhanced BAT activity. AMSE requires clinical validation to be explored as a dietary supplement/functional ingredient with thermogenic effect in food and beverages.

Irisin induces white adipose tissue browning in mice as assessed by magnetic resonance imaging

2021 ◽  
pp. 153537022110060
Author(s):  
Yue Chen ◽  
Jie Ding ◽  
Yufei Zhao ◽  
Shenghong Ju ◽  
Hui Mao ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Adipose Tissue ◽  
Magnetic Resonance ◽  
White Adipose Tissue ◽  
High Fat Diet ◽  
Chow Diet ◽  
Resonance Imaging ◽  
High Fat ◽  
White Adipocytes ◽  
Fat Fraction

This study aimed to track and evaluate the effect of low-dose irisin on the browning of white adipose tissue (WAT) in mice using magnetic resonance imaging (MRI) noninvasively in vivo. Mature white adipocytes extracted from mice were cultured, induced and characterized before being treated by irisin. The volume and fat fraction of WAT were quantified using MRI in normal chow diet and high fat mice after injection of irisin. The browning of cultured white adipocytes and WAT in mice were validated by immunohistochemistry and western blotting for uncoupling protein 1 (UCP1) and deiodinase type II (DIO2). The serum indexes were examined with high fat diet after irisin intervention. UCP1 and DIO2 in adipocytes showed increases responding to the irisin treatment. The size of white adipocytes in mice receiving irisin intervention was reduced. MRI measured volumes and fat fraction of WAT were significantly lower after Irisin treatment. Blood glucose and cholesterol levels were reduced in high fat diet mice after irisin treatment. Irisin intervention exerted browning of WAT, resulting reduction of volume and fat fraction of WAT as measured by MRI. Furthermore, it improved the condition of mice with diet-induced obesity and related metabolic disorders.

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