scholarly journals Comparison of the Allergenicity and Immunogenicity of Camel and Cow’s Milk—A Study in Brown Norway Rats

Nutrients ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 1903 ◽  
Author(s):  
Natalia Maryniak ◽  
Egon Hansen ◽  
Anne-Sofie Ballegaard ◽  
Ana Sancho ◽  
Katrine Bøgh
Keyword(s):  
Protein Composition ◽  
Cross Reactivity ◽  
Camel Milk ◽  
Brown Norway ◽  
Cow’S Milk ◽  
Infant Formulas ◽  
Promising Alternative ◽  
Norway Rat ◽  
Milk Whey ◽  
Cow's Milk

Background: When breastfeeding is impossible or insufficient, the use of cow’s milk-based hypoallergenic infant formulas is an option for infants suffering from or at risk of developing cow’s milk allergy. As the Camelidae family has a large evolutionary distance to the Bovidae family and as camel milk differs from cow’s milk protein composition, there is a growing interest in investigating the suitability of camel milk as an alternative to cow’s milk-based hypoallergenic infant formulas. Methods: The aim of the study was to compare the allergenicity and immunogenicity of camel and cow’s milk as well as investigating their cross-reactivity using a Brown Norway rat model. Rats were immunised intraperitoneally with one of four products: camel milk, cow’s milk, cow’s milk casein or cow’s milk whey fraction. Immunogenicity, sensitising capacity, antibody avidity and cross-reactivity were evaluated by means of different ELISAs. The eliciting capacity was evaluated by an ear swelling test. Results: Camel and cow’s milk showed similarity in their inherent immunogenicity, sensitising and eliciting capacity. Results show that there was a lower cross-reactivity between caseins than between whey proteins from camel and cow’s milk. Conclusions: The study showed that camel and cow’s milk have a low cross-reactivity, indicating a low protein similarity. Results demonstrate that camel milk could be a promising alternative to cow’s milk-based hypoallergenic infant formulas.

scholarly journals Preclinical Brown Norway Rat Models for the Assessment of Infant Formulas in the Prevention and Treatment of Cow’s Milk Allergy

2019 ◽  
Vol 178 (4) ◽  
pp. 307-314 ◽  
Author(s):  
Louise Heydenreich Jensen ◽  
Jeppe Madura Larsen ◽  
Charlotte Bernhard Madsen ◽  
Rune Rønhave Laursen ◽  
Lotte Neergaard Jacobsen ◽  
...  
Keyword(s):  
Cow’S Milk Allergy ◽  
Brown Norway ◽  
Cow’S Milk ◽  
Infant Formulas ◽  
Milk Allergy ◽  
Norway Rat ◽  
Rat Models ◽  
Cow's Milk ◽  
Prevention And Treatment ◽  
Brown Norway Rat

Rabbit antibodies against the folate binding protein from cow's milk. Production, characterization and use for development of an enzyme-linked immunosorbent assay (ELISA)

1986 ◽  
Vol 6 (10) ◽  
pp. 895-900 ◽  
Author(s):  
Mimi Høier-Madsen ◽  
Steen Ingemann Hansen ◽  
Jan Holm
Keyword(s):  
Immunosorbent Assay ◽  
Binding Proteins ◽  
Binding Protein ◽  
Cross Reactivity ◽  
Cow’S Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Folate Binding Protein ◽  
Milk Whey ◽  
Cow's Milk ◽  
Folate Binding Proteins

Rabbit antibodies to purified folate binding protein from cow's milk whey were used for development of a two-site enzyme-linked immunosorbent assay (ELISA) for quantitation of bovine folate binding proteins, The folate binding proteins in human milk and serum showed no cross-reactivity. A partial saturation of purified bovine folate binder with folate gave rise to an increased antigenicity probably due to a ligand (folate)-induced exposure of antigenic sites on the protein.

scholarly journals HPLC Identification of Mare’s Milk and Its Mix with Cow’s Milk

2021 ◽  
Vol 51 (2) ◽  
pp. 402-412
Author(s):  
Vladimir Kurchenko ◽  
Elena Simonenko ◽  
Natalia Sushynskaya ◽  
Tatsiana Halavach ◽  
Andrey Petrov ◽  
...  
Keyword(s):  
Retention Time ◽  
High Performance ◽  
Whey Proteins ◽  
Protein Composition ◽  
Food Product ◽  
Sample Volume ◽  
Cow’S Milk ◽  
Array Detector ◽  
Milk Whey ◽  
Cow's Milk

Introduction. Mare’s milk is a valuable food product with medicinal properties. In combination with cow’s milk, it is used to create new functional foods. Efficient identification of mare’s milk, cow’s milk, and their mixes prevent falsification. Study objects and methods. The protein composition of mare’s and cow’s milk whey and their mixes was analyzed by high performance liquid chromatography (HPLC) using an Agilent 1200 chromatograph with an Agilent G1315C diode array detector. Separation was performed using a column Machinery Nagel C 18 4.6×250, 5 μm. Results and discussion. The standard HPLC method was optimized to analyse whey proteins in the milk samples. The separation of whey proteins included the following optimal parameters: chromatography time = 60 min, linear gradient of acetonitrile concentration = 0–50%, and sample volume for injection = 20 μl. Alpha-lactoalbumin proved to be the protein of mare’s milk and cow’s milk. The retention time of mare’s α-lactoalbumin was 45.16 min, and that of cow’s milk – 40.09 min. The differences in the retention time of α-lactoalbumin were associated with the presence of 33 amino acid substitutions in the primary structure of both milks. The areas of α-lactoalbumin peaks were used to calculate the amount of cow’s milk added to mare’s milk and the related percentage. Conclusion. A HPLC analysis of whey proteins made it possible to determine up to 50 mL of added cow’s milk in 1 liter of mare’s milk.

Manganese Absorption from Human Milk, Cow’s Milk and Infant Formulas

1988 ◽  
pp. 511-512 ◽  
Author(s):  
L. Davidsson ◽  
Å. Cederblad ◽  
B. Lönnerdal ◽  
B. Sandström
Keyword(s):  
Human Milk ◽  
Cow’S Milk ◽  
Infant Formulas ◽  
Cow's Milk

Levels of polychlorinated dibenzodioxins and dibenzofurans in cow's milk and in soy bean derived infant formulas sold in the United States and other countries

Chemosphere ◽  
1989 ◽  
Vol 19 (1-6) ◽  
pp. 913-918 ◽  
Author(s):  
Arnold Schecter ◽  
Peter Fürst ◽  
Christiane Fürst ◽  
Hans-Albert Meemken ◽  
Wilhelm Groebel ◽  
...  
Keyword(s):  
United States ◽  
The United States ◽  
Cow’S Milk ◽  
Infant Formulas ◽  
Cow's Milk ◽  
Polychlorinated Dibenzodioxins

Cow's Milk Proteins Cause Infantile Colic in Breast-Fed Infants: A Double-Blind Crossover Study

PEDIATRICS ◽  
1983 ◽  
Vol 71 (2) ◽  
pp. 268-271 ◽  
Author(s):  
Iréne Jakobsson ◽  
Tor Lindberg
Keyword(s):  
Whey Protein ◽  
Sequential Analysis ◽  
Protein A ◽  
Milk Proteins ◽  
Cow’S Milk ◽  
Infantile Colic ◽  
Double Blind ◽  
Milk Whey ◽  
Cow's Milk ◽  
Breast Fed

Sixty-six mothers of 66 breast-fed infants with infantile colic were put on a diet free from cow's milk. The colic disappeared in 35 infants; it reappeared on at least two challenges (cow's milk to mother) in 23 infants (35%). A double-blind crossover trial with cow's milk whey protein was performed in 16 of these 23 mothers and infants. Six infants had to be taken out of the study for various reasons; of the remaining ten infants, nine reacted with colic after their mothers' intake of whey protein-containing capsules. Sequential analysis showed a high correlation between infantile colic in breast-fed infants and their mothers' consumption of cow's milk protein. A diet free of cow's milk is suggested for the mothers as a first trial of treatment of infantile colic in breast-fed infants.

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