scholarly journals Physical Properties and In Vitro Biocompatible Evaluation of Silicone-Modified Polyurethane Nanofibers and Films

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 367 ◽  
Author(s):  
Chuan Yin ◽  
Sélène Rozet ◽  
Rino Okamoto ◽  
Mikihisa Kondo ◽  
Yasushi Tamada ◽  
...  
Keyword(s):  
Thermal Conductivity ◽  
Cell Proliferation ◽  
Physical Properties ◽  
Water Retention ◽  
Embryonic Fibroblasts ◽  
Water Contact ◽  
Elongation At Break ◽  
In Vitro Biocompatibility ◽  
Short Period

In this study, the physical properties and the biocompatibility of electrospun silicone-modified polyurethane (PUSX) nanofibers were discussed and compared with PUSX films. To investigate the effects of different structures on the physical properties, tensile strength, elongation at break, Young’s modulus, water retention, water contact angle (WCA) and thermal conductivity measurements were performed. To prove the in vitro biocompatibility of the materials, cell adhesion, cell proliferation, and cytotoxicity were studied by NIH3T3 mouse embryonic fibroblasts cells following by lactate dehydrogenase (LDH) analysis. As a conclusion, the mechanical properties, water retention, and WCA were proven to be able to be controlled and improved by adjusting the structure of PUSX. A higher hydrophobicity and lower thermal conductivity were found in PUSX nanofibers compared with polyurethane (PU) nanofibers and films. An in vitro biocompatibility evaluation shows that the cell proliferation can be performed on both PUSX nanofibers and films. However, within a short period, cells prefer to attach and entangle on PUSX nanofibers rather than PUSX films. PUSX nanofibers were proven to be a nontoxic alternative for PU nano-membranes or films in the biomedical field, because of the controllable physical properties and the biocompatibility.

The Effect of Silane on the Biomechanical Properties of PEEK/HA Composite

2015 ◽  
Vol 1125 ◽  
pp. 426-431
Author(s):  
Ahmad Ramli Rashidi ◽  
Mat Uzir Wahit ◽  
M.R. Abdullah ◽  
Mohammed Rafiq Abdul Kadir
Keyword(s):  
Cell Proliferation ◽  
Coupling Agent ◽  
U937 Cell ◽  
Biomechanical Properties ◽  
Screw Extruder ◽  
Elongation At Break ◽  
Single Screw ◽  
Single Screw Extruder ◽  
Bioactive Composites

Bioactive composites containing Polyetheretherketone / Hydroxyapatite (PEEK/HA) were prepared using nanomixer single screw extruder and injection molding. The loading of HA in PEEK matrix was made at different concentrations ranging from 10 wt% to 30 wt%. HA was treated with (3-Aminopropyl) trimethoxysilane, coupling agent, and compared to untreated HA in PEEK/HA composite to evaluate the changes in the biomechanical properties and cytotoxicity. The biomechanical properties including elongation at break and impact properties were assessed. Cell proliferation test was also performed with U937 cell line in the silane treated and untreated PEEK/HA composite. The results showed that silane coupled PEEK-HA had in general improved biomechanical properties than untreated HA and did not show cytotoxicity in vitro.

scholarly journals Enhanced in vitro biocompatibility and osteogenesis of titanium substrates immobilized with dopamine-assisted superparamagnetic Fe 3 O 4 nanoparticles for hBMSCs

2018 ◽  
Vol 5 (8) ◽  
pp. 172033 ◽  
Author(s):  
Zhenfei Huang ◽  
Zhihong Wu ◽  
Bupeng Ma ◽  
Lingjia Yu ◽  
Yu He ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Clinical Applications ◽  
Bone Mesenchymal Stem Cells ◽  
Water Contact ◽  
Alizarin Red ◽  
In Vitro Biocompatibility ◽  
Related Transcription Factor ◽  
Nanoparticle Coatings ◽  
Bio Compatibility

Titanium (Ti) is an ideal bone substitute due to its superior bio-compatibility and remarkable corrosion resistance. However, in order to improve the osteoconduction and osteoinduction capacities in clinical applications, different kinds of surface modifications are typically applied to Ti alloys. In this study, we fabricated a tightly attached polydopamine-assisted Fe 3 O 4 nanoparticle coating on Ti with magnetic properties, aiming to improve the osteogenesis of the Ti substrates. The PDA-assisted Fe 3 O 4 nanoparticle coatings were characterized by scanning electron microscopy, energy dispersive spectroscopy, atomic force microscopy and water contact angle measurements. The cell attachment and proliferation rate of the human bone mesenchymal stem cells (hBMSCs) on the Ti surface significantly improved with the Fe 3 O 4 /PDA coating when compared with the pure Ti without a coating. Furthermore, the results of in vitro alkaline phosphatase (ALP) activity at 7 and 14 days and alizarin red S staining at 14 days showed that the Fe 3 O 4 /PDA coating on Ti promoted the osteogenic differentiation of hBMSCs. Moreover, hBMSCs co-cultured with the Fe 3 O 4 /PDA-coated Ti for approximately 14 days also exhibited a significantly higher mRNA expression level of ALP, osteocalcin and runt-related transcription factor-2 (RUNX2). Our in vitro results revealed that the present PDA-assisted Fe 3 O 4 nanoparticle surface coating is an innovative method for Ti surface modification and shows great potential for clinical applications.

scholarly journals Study On The Effect of Tunicate Cellulose Nanocrystals in The Preparation of Sodium Alginate-Based Enteric Capsule

2021 ◽  
Author(s):  
Dezhong Xu ◽  
Yanan Cheng ◽  
Shuai Wu ◽  
Qiuxia Zou ◽  
Ajoy Kanti Mondal ◽  
...  
Keyword(s):  
Contact Angle ◽  
Aspect Ratio ◽  
Tensile Stress ◽  
Sodium Alginate ◽  
Control Sample ◽  
Maximum Tensile Stress ◽  
Water Contact ◽  
Elongation At Break ◽  
Enteric Capsule

Abstract In this work, the tunicate cellulose nanocrystal (tCNC) was extracted from tunicate by bleaching and acid hydrolysis. It was used as filler in the preparation of sodium alginate-based enteric capsule. The addition of tCNC with high aspect ratio (65) rendered the enteric capsule excellent physical properties. Compared with the control sample, when the addition of tCNC was 10% (wt), the water contact angle of the capsule was enhanced by 46.0%, the opacity was increased by 356.8%, the maximum tensile stress was increased by 142.6%, the modulus of elasticity was increased by 240.3%, and the elongation at break was increased by 133.8%. In the in vitro degradation experiments, the capsule hardly degraded in the gastric environment (pH 1.2), while in the intestinal environment (pH 6.8), the degradation became slower with the increase of tCNC content, which was consistent with the properties of enteric capsule. This research developed a new direction for the application of tCNC in the pharmaceutical material productions.

scholarly journals 3D-Printed Poly(ε-Caprolactone)/Hydroxyapatite Scaffolds Modified with Alkaline Hydrolysis Enhance Osteogenesis In Vitro

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 257
Author(s):  
Sangbae Park ◽  
Jae Eun Kim ◽  
Jinsub Han ◽  
Seung Jeong ◽  
Jae Woon Lim ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Surface Characteristics ◽  
Water Soluble ◽  
Great Promise ◽  
Tetrazolium Salt ◽  
Water Contact ◽  
Alizarin Red ◽  
3D Printed ◽  
O2 Plasma

The 3D-printed bioactive ceramic incorporated Poly(ε-caprolactone) (PCL) scaffolds show great promise as synthetic bone graft substitutes. However, 3D-printed scaffolds still lack adequate surface properties for cells to be attached to them. In this study, we modified the surface characteristics of 3D-printed poly(ε-caprolactone)/hydroxyapatite scaffolds using O2 plasma and sodium hydroxide. The surface property of the alkaline hydrolyzed and O2 plasma-treated PCL/HA scaffolds were evaluated using field-emission scanning microscopy (FE-SEM), Alizarin Red S (ARS) staining, and water contact angle analysis, respectively. The in vitro behavior of the scaffolds was investigated using human dental pulp-derived stem cells (hDPSCs). Cell proliferation of hDPSCs on the scaffolds was evaluated via immunocytochemistry (ICC) and water-soluble tetrazolium salt (WST-1) assay. Osteogenic differentiation of hDPSCs on the scaffolds was further investigated using ARS staining and Western blot analysis. The result of this study shows that alkaline treatment is beneficial for exposing hydroxyapatite particles embedded in the scaffolds compared to O2 plasma treatment, which promotes cell proliferation and differentiation of hDPSCs.

scholarly journals Low Molecular Weight and Polymeric Modifiers as Toughening Agents in Poly(3-Hydroxybutyrate) Films

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2446
Author(s):  
Adriana Nicoleta Frone ◽  
Cristian Andi Nicolae ◽  
Mihaela Carmen Eremia ◽  
Vlad Tofan ◽  
Marius Ghiurea ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Photoelectron Spectroscopy ◽  
Biomedical Applications ◽  
Low Molecular Weight ◽  
Elongation At Break ◽  
Force Microscopy ◽  
In Vitro Biocompatibility ◽  
Atomic Force ◽  
Lower Temperature

The inherent brittleness of poly(3-hydroxybutyrate) (PHB) prevents its use as a substitute of petroleum-based polymers. Low molecular weight plasticizers, such as tributyl 2-acetyl citrate (TAC), cannot properly solve this issue. Herein, PHB films were obtained using a biosynthesized poly(3-hydroxyoctanoate) (PHO) and a commercially available TAC as toughening agents. The use of TAC strongly decreased the PHB thermal stability up to 200 °C due to the loss of low boiling point plasticizer, while minor weight loss was noticed at this temperature for the PHB-PHO blend. Both agents shifted the glass transition temperature of PHB to a lower temperature, the effect being more pronounced for TAC. The elongation at break of PHB increased by 700% after PHO addition and by only 185% in the case of TAC; this demonstrates an important toughening effect of the polymeric modifier. Migration of TAC to the upper surface of the films and no sign of migration in the case of PHO were highlighted by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) results. In vitro biocompatibility tests showed that all the PHB films are non-toxic towards L929 cells and have no proinflammatory immune response. The use of PHO as a toughening agent in PHB represents an attractive solution to its brittleness in the case of packaging and biomedical applications while conserving its biodegradability and biocompatibility.

scholarly journals Activation of the mTOR/ Akt pathway in thymic epithelial cells derived from thymomas

2018 ◽  
Author(s):  
Jean-Michel Maury ◽  
Claire Merveilleux du Vignaux ◽  
Gabrielle Drevet ◽  
Virginie Zarza ◽  
Lara Chalabreysse ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cells ◽  
Cell System ◽  
Mtor Pathway ◽  
Thymic Epithelial Cells ◽  
Thymic Epithelial Tumors ◽  
Molecular Abnormalities ◽  
Short Period ◽  
Thymic Tumors

AbstractThe pathogenesis of thymic epithelial tumors remains poorly elucidated. The PI3K/Akt/mTOR pathway plays a key role in various cancers; interestingly, several phase I/II study reported a positive effect of mTOR inhibitors in disease control in thymoma patients. A major limit for deciphering cellular and molecular events leading to the transformation of thymic epithelial cells or for testing drug candidates is the lack of reliable in vitro cell systemWe analyzed protein expression and activation of key players of the Akt/mTOR pathway namely Akt, mTOR, and P70S6K in thirteen A, B and AB thymomas as well as in normal thymuses. While only Akt and the phospho-Akt were expressed in normal thymuses, both Akt and mTOR were activated, with B2 thymomas expressing higher level of activated phospho-Akt than A or AB subtypes. Phospho-P70S6K was expressed in all thymic tumors whatever their subtypes, and absent in normal thymus. Interestingly, in primary thymic epithelial cells maintained for short period of time after their derivation from seven AB and B thymomas, we report the activation of Akt; mTOR and P70S6. Finally, we analyzed the effect of mTOR inhibitor on thymoma derived epithelial cells and showed that rapamycin (100 nM/ ml) significantly reduced cell proliferation.Our results suggest that the activation of the Akt/ mTOR pathway might participate to the cell proliferation associated with tumor growth. Ultimately, our data enhance the potential role of thymic epithelial cells derived from tissue specimens forin vitroexploration of molecular abnormalities specific to rare thymic tumors.

scholarly journals Evaluation of the In Vitro Biocompatibility of PEDOT:Nafion Coatings

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 2022
Author(s):  
Sonia Guzzo ◽  
Stefano Carli ◽  
Barbara Pavan ◽  
Alice Lunghi ◽  
Mauro Murgia ◽  
...  
Keyword(s):  
Neutral Red ◽  
In Vitro Cytotoxicity ◽  
Water Contact ◽  
Promising Alternative ◽  
Force Microscopy ◽  
In Vitro Biocompatibility ◽  
Angle Measurements ◽  
Organic Bioelectronics

Poly(3,4-ethylenedioxythiophene)-Nafion (PEDOT:Nafion) is emerging as a promising alternative to PEDOT-polystyrene sulfonate (PEDOT:PSS) in organic bioelectronics. However, the biocompatibility of PEDOT:Nafion has not been investigated to date, limiting its deployment toward in vivo applications such as neural recording and stimulation. In the present study, the in vitro cytotoxicity of PEDOT:Nafion coatings, obtained by a water-based PEDOT:Nafion formulation, was evaluated using a primary cell culture of rat fibroblasts. The surface of PEDOT:Nafion coating was characterized by Atomic Force Microscopy (AFM) and water contact angle measurements. Fibroblasts adhesion and morphology was investigated by scanning electron microscopy (SEM) and AFM measurements. Cell proliferation was assessed by fluorescence microscopy, while cell viability was quantified by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), lactate dehydrogenase (LDH) and neutral red assays. The results showed that PEDOT:Nafion coatings obtained by the water dispersion were not cytotoxic, making the latter a reliable alternative to PEDOT:PSS dispersion, especially in terms of chronic in vivo applications.

scholarly journals Flat Die Extruded Biocompatible Poly(Lactic Acid) (PLA)/Poly(Butylene Succinate) (PBS) Based Films

Polymers ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1857 ◽  
Author(s):  
Vito Gigante ◽  
Maria-Beatrice Coltelli ◽  
Alessandro Vannozzi ◽  
Luca Panariello ◽  
Alessandra Fusco ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Poly Lactic Acid ◽  
Butylene Succinate ◽  
Elongation At Break ◽  
Melt Strength ◽  
In Vitro Biocompatibility ◽  
Extruded Films ◽  
Acrylic Copolymers ◽  
Sanitary Products

Biodegradable polymers are promising materials for films and sheets used in many widely diffused applications like packaging, personal care products and sanitary products, where the synergy of high biocompatibility and reduced environmental impact can be particularly significant. Plasticized poly(lactic acid) (PLA)/poly(butylene succinate) (PBS) blend-based films, showing high cytocompatibility and improved flexibility than pure PLA, were prepared by laboratory extrusion and their processability was controlled by the use of a few percent of a commercial melt strength enhancer, based on acrylic copolymers and micro-calcium carbonate. The melt strength enhancer was also found effective in reducing the crystallinity of the films. The process was upscaled by producing flat die extruded films in which elongation at break and tear resistance were improved than pure PLA. The in vitro biocompatibility, investigated through the contact of flat die extruded films with cells, namely, keratinocytes and mesenchymal stromal cells, resulted improved with respect to low density polyethylene (LDPE). Moreover, the PLA-based materials were able to affect immunomodulatory behavior of cells and showed a slight indirect anti-microbial effect. These properties could be exploited in several applications, where the contact with skin and body is relevant.

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