scholarly journals Enhanced in vitro biocompatibility and osteogenesis of titanium substrates immobilized with dopamine-assisted superparamagnetic Fe 3 O 4 nanoparticles for hBMSCs

2018 ◽  
Vol 5 (8) ◽  
pp. 172033 ◽  
Author(s):  
Zhenfei Huang ◽  
Zhihong Wu ◽  
Bupeng Ma ◽  
Lingjia Yu ◽  
Yu He ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Clinical Applications ◽  
Bone Mesenchymal Stem Cells ◽  
Water Contact ◽  
Alizarin Red ◽  
In Vitro Biocompatibility ◽  
Related Transcription Factor ◽  
Nanoparticle Coatings ◽  
Bio Compatibility

Titanium (Ti) is an ideal bone substitute due to its superior bio-compatibility and remarkable corrosion resistance. However, in order to improve the osteoconduction and osteoinduction capacities in clinical applications, different kinds of surface modifications are typically applied to Ti alloys. In this study, we fabricated a tightly attached polydopamine-assisted Fe 3 O 4 nanoparticle coating on Ti with magnetic properties, aiming to improve the osteogenesis of the Ti substrates. The PDA-assisted Fe 3 O 4 nanoparticle coatings were characterized by scanning electron microscopy, energy dispersive spectroscopy, atomic force microscopy and water contact angle measurements. The cell attachment and proliferation rate of the human bone mesenchymal stem cells (hBMSCs) on the Ti surface significantly improved with the Fe 3 O 4 /PDA coating when compared with the pure Ti without a coating. Furthermore, the results of in vitro alkaline phosphatase (ALP) activity at 7 and 14 days and alizarin red S staining at 14 days showed that the Fe 3 O 4 /PDA coating on Ti promoted the osteogenic differentiation of hBMSCs. Moreover, hBMSCs co-cultured with the Fe 3 O 4 /PDA-coated Ti for approximately 14 days also exhibited a significantly higher mRNA expression level of ALP, osteocalcin and runt-related transcription factor-2 (RUNX2). Our in vitro results revealed that the present PDA-assisted Fe 3 O 4 nanoparticle surface coating is an innovative method for Ti surface modification and shows great potential for clinical applications.

scholarly journals Osteogenic Properties of 3D-Printed Silica-Carbon-Calcite Composite Scaffolds: Novel Approach for Personalized Bone Tissue Regeneration

2021 ◽  
Vol 22 (2) ◽  
pp. 475
Author(s):  
Parastoo Memarian ◽  
Francesco Sartor ◽  
Enrico Bernardo ◽  
Hamada Elsayed ◽  
Batur Ercan ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Bone Tissue Regeneration ◽  
Hemolysis Assay ◽  
Novel Approach ◽  
Related Transcription Factor ◽  
Diphenyl Tetrazolium Bromide

Carbon enriched bioceramic (C-Bio) scaffolds have recently shown exceptional results in terms of their biological and mechanical properties. The present study aims at assessing the ability of the C-Bio scaffolds to affect the commitment of canine adipose-derived mesenchymal stem cells (cAD-MSCs) and investigating the influence of carbon on cell proliferation and osteogenic differentiation of cAD-MSCs in vitro. The commitment of cAD-MSCs to an osteoblastic phenotype has been evaluated by expression of several osteogenic markers using real-time PCR. Biocompatibility analyses through 3-(4,5-dimethyl- thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), lactate dehydrogenase (LDH) activity, hemolysis assay, and Ames test demonstrated excellent biocompatibility of both materials. A significant increase in the extracellular alkaline phosphatase (ALP) activity and expression of runt-related transcription factor (RUNX), ALP, osterix (OSX), and receptor activator of nuclear factor kappa-Β ligand (RANKL) genes was observed in C-Bio scaffolds compared to those without carbon (Bio). Scanning electron microscopy (SEM) demonstrated excellent cell attachment on both material surfaces; however, the cellular layer on C-Bio fibers exhibited an apparent secretome activity. Based on our findings, graphene can improve cell adhesion, growth, and osteogenic differentiation of cAD-MSCs in vitro. This study proposed carbon as an additive for a novel three-dimensional (3D)-printable biocompatible scaffold which could become the key structural material for bone tissue reconstruction.

Osteogenic Potential of Autogenous Bone Grafts Harvested with Four Different Surgical Techniques

2011 ◽  
Vol 90 (12) ◽  
pp. 1428-1433 ◽  
Author(s):  
R.J. Miron ◽  
E. Hedbom ◽  
N. Saulacic ◽  
Y. Zhang ◽  
A. Sculean ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Surgical Techniques ◽  
Bone Grafts ◽  
Osteogenic Potential ◽  
Autogenous Bone ◽  
Mrna Levels ◽  
Alizarin Red ◽  
Osteogenic Response ◽  
Autogenous Bone Grafts

The osteogenic potential of autogenous bone grafts is superior to that of allografts and xenografts because of their ability to release osteoinductive growth factors and provide a natural osteoconductive surface for cell attachment and growth. In this in vitro study, autogenous bone particles were harvested by four commonly used techniques and compared for their ability to promote an osteogenic response. Primary osteoblasts were isolated and seeded on autogenous bone grafts prepared from the mandibles of miniature pigs with a bone mill, piezo-surgery, bone scraper, and bone drill (bone slurry). The osteoblast cultures were compared for their ability to promote cell attachment, proliferation, and differentiation. After 4 and 8 hrs, significantly higher cell numbers were associated with bone mill and bone scraper samples compared with those acquired by bone slurry and piezo-surgery. Similar patterns were consistently observed up to 5 days. Furthermore, osteoblasts seeded on bone mill and scraper samples expressed significantly elevated mRNA levels of collagen, osteocalcin, and osterix at 3 and 14 days and produced more mineralized tissue as assessed by alizarin red staining. These results suggest that the larger bone graft particles produced by bone mill and bone scraper techniques have a higher osteogenic potential than bone slurry and piezo-surgery.

scholarly journals Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 497 ◽  
Author(s):  
Moumita Ghosh ◽  
Michal Halperin-Sternfeld ◽  
Itzhak Grinberg ◽  
Lihi Adler-Abramovich
Keyword(s):  
Extracellular Matrix ◽  
Bone Regeneration ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Composite Hydrogel ◽  
Bone Tissue Regeneration ◽  
Pcr Analysis ◽  
Alizarin Red ◽  
In Vitro Biocompatibility

The high demand for tissue engineering scaffolds capable of inducing bone regeneration using minimally invasive techniques prompts the need for the development of new biomaterials. Herein, we investigate the ability of Alginate incorporated with the fluorenylmethoxycarbonyl-diphenylalanine (FmocFF) peptide composite hydrogel to serve as a potential biomaterial for bone regeneration. We demonstrate that the incorporation of the self-assembling peptide, FmocFF, in sodium alginate leads to the production of a rigid, yet injectable, hydrogel without the addition of cross-linking agents. Scanning electron microscopy reveals a nanofibrous structure which mimics the natural bone extracellular matrix. The formed composite hydrogel exhibits thixotropic behavior and a high storage modulus of approximately 10 kPA, as observed in rheological measurements. The in vitro biocompatibility tests carried out with MC3T3-E1 preosteoblast cells demonstrate good cell viability and adhesion to the hydrogel fibers. This composite scaffold can induce osteogenic differentiation and facilitate calcium mineralization, as shown by Alizarin red staining, alkaline phosphatase activity and RT-PCR analysis. The high biocompatibility, excellent mechanical properties and similarity to the native extracellular matrix suggest the utilization of this hydrogel as a temporary three-dimensional cellular microenvironment promoting bone regeneration.

Enhanced Biocompatibility of GPC by Ion Implantation and Deposition

2005 ◽  
Vol 908 ◽  
Author(s):  
Robert Lee Zimmerman ◽  
Ismet Gürhan ◽  
Claudiu I. Muntele ◽  
Daryush Ila ◽  
Feyzan Özdal-Kurt ◽  
...  
Keyword(s):  
Ion Implantation ◽  
Biomedical Applications ◽  
Heart Valves ◽  
Cell Attachment ◽  
Blood Stream ◽  
Silver Deposition ◽  
In Vitro Biocompatibility ◽  
Artificial Heart Valves ◽  
Model Cell

AbstractBiocompatible Glassy Polymeric Carbon (GPC) is used for artificial heart valves and in other biomedical applications. Although it is ideally suited for implants in the blood stream, tissue that normally forms around the moving parts of a GPC heart valve sometimes loses adhesion and creates embolisms downstream. Here we compare silver ion implantation and silver deposition, each of which strongly inhibits cell attachment on GPC. Inhibition of cell adhesion is a desirable improvement to current GPC cardiac implants. In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that traces of silver can favorably influence the surface of GPC for biomedical applications.

Persistent Inhibition of Cell Growth on Silver Implanted Glassy Polymeric Carbon

2006 ◽  
Vol 950 ◽  
Author(s):  
Robert L. Zimmerman ◽  
Ismet Gürhan ◽  
F. Ozdal-Kurt ◽  
B. H. Sen ◽  
Marcello Rodrigues ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Attachment ◽  
Surface Deposition ◽  
Near Surface ◽  
In Vitro Biocompatibility ◽  
Model Cell ◽  
One Year ◽  
Polymeric Carbon ◽  
Argon Ion

ABSTRACTWe have shown that silver ion implantation or argon ion assisted surface deposition of silver inhibits cell growth on Glassy Polymeric Carbon (GPC), a desirable improvement of current cardiac implants. In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that near surface implantation of silver in GPC can completely inhibit cell attachment on implanted areas while leaving adjacent areas vulnerable to strong cell adhesion. After cleaning and sterilization and more than one year in physiologic solution, the silver implanted GPC persists in inhibiting cell attachment.

scholarly journals Physical Properties and In Vitro Biocompatible Evaluation of Silicone-Modified Polyurethane Nanofibers and Films

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 367 ◽  
Author(s):  
Chuan Yin ◽  
Sélène Rozet ◽  
Rino Okamoto ◽  
Mikihisa Kondo ◽  
Yasushi Tamada ◽  
...  
Keyword(s):  
Thermal Conductivity ◽  
Cell Proliferation ◽  
Physical Properties ◽  
Water Retention ◽  
Embryonic Fibroblasts ◽  
Water Contact ◽  
Elongation At Break ◽  
In Vitro Biocompatibility ◽  
Short Period

In this study, the physical properties and the biocompatibility of electrospun silicone-modified polyurethane (PUSX) nanofibers were discussed and compared with PUSX films. To investigate the effects of different structures on the physical properties, tensile strength, elongation at break, Young’s modulus, water retention, water contact angle (WCA) and thermal conductivity measurements were performed. To prove the in vitro biocompatibility of the materials, cell adhesion, cell proliferation, and cytotoxicity were studied by NIH3T3 mouse embryonic fibroblasts cells following by lactate dehydrogenase (LDH) analysis. As a conclusion, the mechanical properties, water retention, and WCA were proven to be able to be controlled and improved by adjusting the structure of PUSX. A higher hydrophobicity and lower thermal conductivity were found in PUSX nanofibers compared with polyurethane (PU) nanofibers and films. An in vitro biocompatibility evaluation shows that the cell proliferation can be performed on both PUSX nanofibers and films. However, within a short period, cells prefer to attach and entangle on PUSX nanofibers rather than PUSX films. PUSX nanofibers were proven to be a nontoxic alternative for PU nano-membranes or films in the biomedical field, because of the controllable physical properties and the biocompatibility.

Role of Osteogenesis of MC3T3-E1 Induced by Demineralized Tooth Block with Composite Acids: An In Vitro Study

2020 ◽  
Vol 10 (8) ◽  
pp. 1085-1093
Author(s):  
Liu-Zhu Jin ◽  
Xiao-Qian Gu ◽  
Jing-Jing Li ◽  
Yuan Ma ◽  
Lu Cai ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Cell Attachment ◽  
Alizarin Red S ◽  
Permanent Teeth ◽  
Alizarin Red ◽  
Superior Result ◽  
Significant Difference ◽  
Group A ◽  
Demineralized Dentin

More and more research had focused on the osteogenesis of demineralized dentin in clinic, especially when the first application of deminerized dentin in 2008. The study tried to compare the osteogenetic ability of the demineralized dentin block, which were processed two different regents by VacuaSonic system. The extracted human permanent teeth were demineralized by two different methods. Then the MC3T3-E1 cells were invited to culture on the surface of these demineralized dentin blocks (DDB). The cell attachment, proliferation and differentiation were tested. Adhesion of MC3T3-E1 on DDM was observed using scanning electron microscopy and confocal test, the Alizarin Red S, ALP activity, and the protein of BMP-2/-7 and OCN were employed to confirm the level of cell differentiation. The P value was set at 0.05. The microfilaments established a good contact and formed a network in Group A. The Group A had more full cytoskeleton and actin stretched more obviously than Group C, the number of cells on three scaffolds were difference (p < 0 05). The MTT results showed no cytotoxicity in all experiment groups, and Group C had a significant difference in cell proliferation than other groups (p < 0 05) except for day 1. While when related to the cell differentiation, Group A showed a similar result with Group C, but in Alizarin Red S, Group A had a superior result (p < 0 05). The tooth dentin scaffold processed with composite acids in Group A presents the superiority in osteoconduction and preferable osteogenesis ability, which could be an alternative method to process the tooth scaffold.

scholarly journals 3D-Printed Poly(ε-Caprolactone)/Hydroxyapatite Scaffolds Modified with Alkaline Hydrolysis Enhance Osteogenesis In Vitro

Polymers ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 257
Author(s):  
Sangbae Park ◽  
Jae Eun Kim ◽  
Jinsub Han ◽  
Seung Jeong ◽  
Jae Woon Lim ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Surface Characteristics ◽  
Water Soluble ◽  
Great Promise ◽  
Tetrazolium Salt ◽  
Water Contact ◽  
Alizarin Red ◽  
3D Printed ◽  
O2 Plasma

The 3D-printed bioactive ceramic incorporated Poly(ε-caprolactone) (PCL) scaffolds show great promise as synthetic bone graft substitutes. However, 3D-printed scaffolds still lack adequate surface properties for cells to be attached to them. In this study, we modified the surface characteristics of 3D-printed poly(ε-caprolactone)/hydroxyapatite scaffolds using O2 plasma and sodium hydroxide. The surface property of the alkaline hydrolyzed and O2 plasma-treated PCL/HA scaffolds were evaluated using field-emission scanning microscopy (FE-SEM), Alizarin Red S (ARS) staining, and water contact angle analysis, respectively. The in vitro behavior of the scaffolds was investigated using human dental pulp-derived stem cells (hDPSCs). Cell proliferation of hDPSCs on the scaffolds was evaluated via immunocytochemistry (ICC) and water-soluble tetrazolium salt (WST-1) assay. Osteogenic differentiation of hDPSCs on the scaffolds was further investigated using ARS staining and Western blot analysis. The result of this study shows that alkaline treatment is beneficial for exposing hydroxyapatite particles embedded in the scaffolds compared to O2 plasma treatment, which promotes cell proliferation and differentiation of hDPSCs.

Fabrication and evaluation of polylactic acid/pectin composite scaffold via freeze extraction for tissue engineering

2020 ◽  
Vol 40 (5) ◽  
pp. 421-431
Author(s):  
Mohd Syahir Anwar Hamzah ◽  
Saiful Izwan Abd Razak ◽  
Mohammed Rafiq Abdul Kadir ◽  
Siti Pauliena Mohd Bohari ◽  
Nadirul Hasraf Mat Nayan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Polylactic Acid ◽  
Cell Attachment ◽  
Porous Scaffold ◽  
Composite Scaffold ◽  
Engineering Material ◽  
Water Contact ◽  
Good Biocompatibility ◽  
Proliferation In Vitro

AbstractThis work reports the fabrication and characterizations of porous scaffold made up of polylactic acid (PLA) with the inclusion of pectin (1, 3, 5, 7, 9, 11 wt%) for potential tissue engineering material. The composite scaffold was prepared using a facile method of freeze extraction. Based on the physical evaluations, the scaffold was suggested to be optimum at 5 wt% of pectin loading. Water contact angle of the scaffold was significantly reduced to 46.5o with the inclusion of 5 wt% of pectin. Morphological and topographic of the PLA scaffold revealed that the pectin induced more porous structure and its surface became rougher which was suitable for cell attachment and proliferation. In vitro studies of the PLA/pectin composite scaffold using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidelt (MTT) assay revealed good biocompatibility whereas Live-Dead kit assay resulted in 91% cell viability after 7 days of incubation.

scholarly journals Beraprost ameliorates postmenopausal osteoporosis by regulating Nedd4-induced Runx2 ubiquitination

2021 ◽  
Vol 12 (5) ◽  
Author(s):  
Huo-Liang Zheng ◽  
Wen-Ning Xu ◽  
Wen-Sheng Zhou ◽  
Run-Ze Yang ◽  
Peng-Bo Chen ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Mass ◽  
Postmenopausal Osteoporosis ◽  
Vital Role ◽  
Therapeutic Drug ◽  
Dependent Manner ◽  
Bone Mesenchymal Stem Cells ◽  
Related Transcription Factor ◽  
Neuronal Precursor Cell

AbstractBone health requires adequate bone mass, which is maintained by a critical balance between bone resorption and formation. In our study, we identified beraprost as a pivotal regulator of bone formation and resorption. The administration of beraprost promoted differentiation of mouse bone mesenchymal stem cells (M-BMSCs) through the PI3K–AKT pathway. In co-culture, osteoblasts stimulated with beraprost inhibited osteoclastogenesis in a rankl-dependent manner. Bone mass of p53 knockout mice remained stable, regardless of the administration of beraprost, indicating that p53 plays a vital role in the bone mass regulation by beraprost. Mechanistic in vitro studies showed that p53 binds to the promoter region of neuronal precursor cell-expressed developmentally downregulated 4 (Nedd4) to promote its transcription. As a ubiquitinating enzyme, Nedd4 binds to runt-related transcription factor 2 (Runx2), which results in its ubiquitination and subsequent degradation. These data indicate that the p53–Nedd4–Runx2 axis is an effective regulator of bone formation and highlight the potential of beraprost as a therapeutic drug for postmenopausal osteoporosis.

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