scholarly journals Electrospun Filaments Embedding Bioactive Glass Particles with Ion Release and Enhanced Mineralization

Nanomaterials ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 182 ◽  
Author(s):  
Francesca Serio ◽  
Marta Miola ◽  
Enrica Vernè ◽  
Dario Pisignano ◽  
Aldo Boccaccini ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Poly Lactic Acid ◽  
Uniaxial Tensile ◽  
Ion Release ◽  
Neat Polymer ◽  
Aligned Arrays ◽  
Uniform Dispersion ◽  
Biodegradable Poly ◽  
Uniaxial Tensile Stress

Efforts in tissue engineering aim at creating scaffolds that mimic the physiological environment with its structural, topographical and mechanical properties for restoring the function of damaged tissue. In this study we introduce composite fibres made by a biodegradable poly(lactic acid) (PLLA) matrix embedding bioactive silica-based glass particles (SBA2). Electrospinning is performed to achieve porous PLLA filaments with uniform dispersion of bioactive glass powder. The obtained composite fibres show in aligned arrays significantly increased elastic modulus compared with that of neat polymer fibres during uniaxial tensile stress. Additionally, the SBA2 bioactivity is preserved upon encapsulation as highlighted by the promoted deposition of hydroxycarbonate apatite (HCA) upon immersion in simulated body fluid solutions. HCA formation is sequential to earlier processes of polymer erosion and ion release leading to acidification of the surrounding solution environment. These findings suggest PLLA-SBA2 fibres as a composite, multifunctional system which might be appealing for both bone and soft tissue engineering applications.

scholarly journals Characterization of biodegradable poly(lactic acid) porous scaffolds prepared using selective enzymatic degradation for tissue engineering

RSC Advances ◽  
2017 ◽  
Vol 7 (54) ◽  
pp. 34063-34070 ◽  
Author(s):  
Ziqi Guo ◽  
Cheng Yang ◽  
Zuping Zhou ◽  
Shan Chen ◽  
Fan Li
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Enzymatic Degradation ◽  
Poly Lactic Acid ◽  
Porous Scaffolds ◽  
Sem Images ◽  
Biodegradable Poly

SEM images of MEF cells on PLA scaffolds prepared by selective enzymatic degradation after 7 days of culture. The results demonstrated that MEF cells attached more easily to the surface than in the interior of the PLA scaffolds.

scholarly journals Hypoxia-mimicking mesoporous bioactive glass scaffolds with controllable cobalt ion release for bone tissue engineering

Biomaterials ◽  
2012 ◽  
Vol 33 (7) ◽  
pp. 2076-2085 ◽  
Author(s):  
Chengtie Wu ◽  
Yinghong Zhou ◽  
Wei Fan ◽  
Pingping Han ◽  
Jiang Chang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Ion Release ◽  
Cobalt Ion ◽  
Mesoporous Bioactive Glass

scholarly journals Electrospun PCL Fiber Mats Incorporating Multi-Targeted B and Co Co-Doped Bioactive Glass Nanoparticles for Angiogenesis

Materials ◽  
2020 ◽  
Vol 13 (18) ◽  
pp. 4010 ◽  
Author(s):  
Si Chen ◽  
Dagmar Galusková ◽  
Hana Kaňková ◽  
Kai Zheng ◽  
Martin Michálek ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Soft Tissue ◽  
Bioactive Glass ◽  
Sol Gel ◽  
Spherical Shape ◽  
Biologically Active ◽  
Ion Release ◽  
Fiber Mats ◽  
Young’S Moduli ◽  
Co Doped

Vascularization is necessary in tissue engineering to keep adequate blood supply in order to maintain the survival and growth of new tissue. The synergy of biologically active ions with multi-target activity may lead to superior angiogenesis promotion in comparison to single-target approaches but it has been rarely investigated. In this study, polycaprolactone (PCL) fiber mats embedded with B and Co co-doped bioactive glass nanoparticles (BCo.BGNs) were fabricated as a tissue regeneration scaffold designed for promoting angiogenesis. BCo.NBGs were successfully prepared with well-defined spherical shape using a sol-gel method. The PCL fiber mats embedding co-doped bioactive glass nanoparticles were fabricated by electrospinning using benign solvents. The Young’s moduli of the nanoparticle containing PCL fiber mats were similar to those of the neat fiber mats and suitable for scaffolds utilized in soft tissue repair approaches. The mats also showed non-cytotoxicity to ST-2 cells. PCL fiber mats containing BCo.BGNs with a relatively high content of B and Co promoted the secretion of vascular endothelial growth factor to a greater extent than PCL fiber mats with a relatively low B and Co contents, which demonstrates the potential of dual ion release (B and Co) from bioactive glasses to enhance angiogenesis in soft tissue engineering.

Development of three-dimensional printing filaments based on poly(lactic acid)/hydroxyapatite composites with potential for tissue engineering

2021 ◽  
pp. 002199832098856
Author(s):  
Marcela Piassi Bernardo ◽  
Bruna Cristina Rodrigues da Silva ◽  
Luiz Henrique Capparelli Mattoso
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Fused Deposition Modeling ◽  
Three Dimensional ◽  
Poly Lactic Acid ◽  
Scanning Calorimetry ◽  
Three Dimensional Printing ◽  
Fused Deposition ◽  
Deposition Modeling ◽  
3D Printed

Injured bone tissues can be healed with scaffolds, which could be manufactured using the fused deposition modeling (FDM) strategy. Poly(lactic acid) (PLA) is one of the most biocompatible polymers suitable for FDM, while hydroxyapatite (HA) could improve the bioactivity of scaffold due to its chemical composition. Therefore, the combination of PLA/HA can create composite filaments adequate for FDM and with high osteoconductive and osteointegration potentials. In this work, we proposed a different approache to improve the potential bioactivity of 3D printed scaffolds for bone tissue engineering by increasing the HA loading (20-30%) in the PLA composite filaments. Two routes were investigated regarding the use of solvents in the filament production. To assess the suitability of the FDM-3D printing process, and the influence of the HA content on the polymer matrix, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC) and scanning electron microscopy (SEM) were performed. The HA phase content of the composite filaments agreed with the initial composite proportions. The wettability of the 3D printed scaffolds was also increased. It was shown a greener route for obtaining composite filaments that generate scaffolds with properties similar to those obtained by the solvent casting, with high HA content and great potential to be used as a bone graft.

Structure and Properties of Poly(α-hydroxyl acids)/Nano Hydroxyapatite Composite Scaffolds

2002 ◽  
Vol 735 ◽  
Author(s):  
Guobao Wei ◽  
Peter X. Ma
Keyword(s):  
Tissue Engineering ◽  
Pore Structure ◽  
The Body ◽  
Polymer Scaffolds ◽  
Composite Scaffolds ◽  
Average Pore ◽  
Thermally Induced ◽  
Tissue Engineering Approach ◽  
Biodegradable Poly ◽  
Organ Failures

ABSTRACTTissue losses and organ failures resulting from injuries or diseases remain frequent and serious health problems despite great advances in medical technologies. Transplantation and reconstructive surgeries are seriously challenged by donor tissue shortage. We take a tissue engineering approach to design 3D scaffolds for cells to grow and synthesize new tissues. The scaffolds are biodegradable and will be absorbed after fulfilling the purpose as 3D templates, leaving nothing foreign in the body. To better mimic natural bone structurally, mechanically and biologically, nano-sized hydroxyapatite particles (N-HAP) were formulated with biodegradable poly(α-hydroxyl acids) to form composite scaffolds with well-controlled pore structures using thermally induced phase separation (TIPS) in this work. The pore structure and mechanical properties of the scaffolds were optimized by the use of multiple solvent systems, different quenching rates and quenching depths. The fabricated scaffolds possessed porosities higher than 90% and average pore sizes ranging from 50 to 500 μm. The scaffolds containing N-HAP maintained open and regular 3D pore structure similar to those of plain polymer scaffolds, implying that N-HAP particles were dispersed within the polymer pore walls of the scaffolds. The addition of N-HAP increased the compressive modulus by 20∼80% over that of plain polymer scaffolds. These results indicate that poly(α-hydroxyl acids)/N-HAP scaffolds may provide excellent 3D substrates for bone tissue engineering.

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