scholarly journals Antimicrobial and Antioxidant Performance of Various Essential Oils and Natural Extracts and Their Incorporation into Biowaste Derived Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Layers Made from Electrospun Ultrathin Fibers

Nanomaterials ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 144 ◽  
Author(s):  
Kelly J. Figueroa-Lopez ◽  
António A. Vicente ◽  
Maria A.M. Reis ◽  
Sergio Torres-Giner ◽  
Jose M. Lagaron

In this research, the antibacterial and antioxidant properties of oregano essential oil (OEO), rosemary extract (RE), and green tea extract (GTE) were evaluated. These active substances were encapsulated into ultrathin fibers of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) derived from fruit waste using solution electrospinning, and the resultant electrospun mats were annealed to produce continuous films. The incorporation of the active substances resulted in PHBV films with a relatively high contact transparency, but it also induced a slightly yellow appearance and increased the films opacity. Whereas OEO significantly reduced the onset of thermal degradation of PHBV, both the RE and GTE-containing PHBV films showed a thermal stability profile that was similar to the neat PHBV film. In any case, all the active PHBV films were stable up to approximately 200 °C. The incorporation of the active substances also resulted in a significant decrease in hydrophobicity. The antimicrobial and antioxidant activity of the films were finally evaluated in both open and closed systems for up to 15 days in order to anticipate the real packaging conditions. The results showed that the electrospun OEO-containing PHBV films presented the highest antimicrobial activity against two strains of food-borne bacteria, as well as the most significant antioxidant performance, ascribed to the films high content in carvacrol and thymol. Therefore, the PHBV films developed in this study presented high antimicrobial and antioxidant properties, and they can be applied as active layers to prolong the shelf life of the foods in biopackaging applications.

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3898
Author(s):  
Surakshi Wimangika Rajapaksha ◽  
Naoto Shimizu

Antioxidant polyphenols in black tea residue are an underused source of bioactive compounds. Microencapsulation can turn them into a valuable functional ingredient for different food applications. This study investigated the potential of using spent black tea extract (SBT) as an active ingredient in food packaging. Free or microencapsulated forms of SBT, using a pectin–sodium caseinate mixture as a wall material, were incorporated in a cassava starch matrix and films developed by casting. The effect of incorporating SBT at different polyphenol contents (0.17% and 0.34%) on the structural, physical, and antioxidant properties of the films, the migration of active compounds into different food simulants and their performance at preventing lipid oxidation were evaluated. The results showed that adding free SBT modified the film structure by forming hydrogen bonds with starch, creating a less elastic film with antioxidant activity (173 and 587 µg(GAE)/g film). Incorporating microencapsulated SBT improved the mechanical properties of active films and preserved their antioxidant activity (276 and 627 µg(GAE)/g film). Encapsulates significantly enhanced the release of antioxidant polyphenols into both aqueous and fatty food simulants. Both types of active film exhibited better barrier properties against UV light and water vapour than the control starch film and delayed lipid oxidation up to 35 d. This study revealed that starch film incorporating microencapsulated SBT can be used as a functional food packaging to protect fatty foods from oxidation.


Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4633
Author(s):  
Aleksandra Ostapiuk ◽  
Łukasz Kurach ◽  
Maciej Strzemski ◽  
Jacek Kurzepa ◽  
Anna Hordyjewska

Silver birch, Betula pendula Roth, is one of the most common trees in Europe. Due to its content of many biologically active substances, it has long been used in medicine and cosmetics, unlike the rare black birch, Betula obscura Kotula. The aim of the study was therefore to compare the antioxidant properties of extracts from the inner and outer bark layers of both birch trees towards the L929 line treated with acetaldehyde. Based on the lactate dehydrogenase test and the MTT test, 10 and 25% concentrations of extracts were selected for the antioxidant evaluation. All extracts at tested concentrations reduced the production of hydrogen peroxide, superoxide anion radical, and 25% extract decreased malonic aldehyde formation in acetaldehyde-treated cells. The chemical composition of bark extracts was accessed by IR and HPLC-PDA methods and surprisingly, revealed a high content of betulin and lupeol in the inner bark extract of B. obscura. Furthermore, IR analysis revealed differences in the chemical composition of the outer bark between black and silver birch extracts, indicating that black birch may be a valuable source of numerous biologically active substances. Further experiments are required to evaluate their potential against neuroinflammation, cancer, viral infections, as well as their usefulness in cosmetology.


Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4390
Author(s):  
Traian Zaharescu ◽  
Ignazio Blanco

In this work the analysis on the stabilization activities of some natural antioxidants (rosemary extract, capsaicin, quercetin or oleanolic acid) is presented. A similar contribution of an inorganic structure—polyhedral oligomeric silsesquioxane (POSS) nanoparticles—is also evaluated. The stabilization effects on the oxidation protection were investigated for several formulations based on ethylene-propylene-diene-terpolymer (EPDM). The samples were examined in pristine state or after γ-irradiation, when the accelerated degradation scission of polymer macromolecules followed by the mitigation of oxidation. Three evaluation procedures: chemiluminescence, FTIR spectroscopy and thermal analysis were applied for the characterization of stability efficiency. The delaying effect of oxidative aging in EPDM matrix is illustrated by the values of activation energy, which are correlated with the type and concentration of embedded compounds. The durability of studied EPDM formulations is discussed for the assessment of material life. The improved behavior of structured hybrids useful for the optimization application regimes is essentially based on the antioxidant properties of polyphenolic components in the cases of natural antioxidants or on the penetration of free radical intermediates into the free volumes of POSS.


Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 327 ◽  
Author(s):  
Frederico V. R. Castro ◽  
Mariana A. Andrade ◽  
Ana Sanches Silva ◽  
Maria Fátima Vaz ◽  
Fernanda Vilarinho

Active packaging is becoming progressively more significant as a response to the dynamic changes in current consumer demand and market tendencies. Active packaging is projected to interact directly with the packaged food or with the headspace within the package with the aim of maintaining or extending product quality and shelf-life. Aiming for sustainability, the potential application as biodegradable films of whey protein concentrate (WPC) was evaluated. Aromatic plant’s extracts present high antioxidant properties, representing an alternative for synthetic food additives. The main objective of this study was to verify the effectiveness of an edible WPC film incorporated with a plant-based extract on retarding the lipid oxidation of fresh salmon. Green tea extract (GTE) was chosen to be incorporated into the active film. Fresh salmon was packaged with the control film (WPC) and with active film (WPC–GTE). The oxidation level of non-packaged samples and packaged samples were tested for different storage times. Four methods were applied to evaluate lipid oxidation state of fresh salmon: peroxide value, p-anisidine value, thiobarbituric acid reactive substances (TBARS) assay, and monitoring of hexanal. The results obtained in this study indicate that the whey protein active film was successfully produced, and it was effective in delaying lipid oxidation of fresh salmon samples until the 14th day of storage.


Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2356
Author(s):  
Kelly Figueroa-Lopez ◽  
Sergio Torres-Giner ◽  
Inmaculada Angulo ◽  
Maria Pardo-Figuerez ◽  
Jose Escuin ◽  
...  

Active multilayer films based on polyhydroxyalkanoates (PHAs) with and without high barrier coatings of cellulose nanocrystals (CNCs) were herein successfully developed. To this end, an electrospun antimicrobial hot-tack layer made of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) derived from cheese whey, a by-product from the dairy industry, was deposited on a previously manufactured blown film of commercial food contact PHA-based resin. A hybrid combination of oregano essential oil (OEO) and zinc oxide nanoparticles (ZnONPs) were incorporated during the electrospinning process into the PHBV nanofibers at 2.5 and 2.25 wt%, respectively, in order to provide antimicrobial properties. A barrier CNC coating was also applied by casting from an aqueous solution of nanocellulose at 2 wt% using a rod at 1m/min. The whole multilayer structure was thereafter assembled in a pilot roll-to-roll laminating system, where the blown PHA-based film was located as the outer layers while the electrospun antimicrobial hot-tack PHBV layer and the barrier CNC coating were placed as interlayers. The resultant multilayer films, having a final thickness in the 130–150 µm range, were characterized to ascertain their potential in biodegradable food packaging. The multilayers showed contact transparency, interlayer adhesion, improved barrier to water and limonene vapors, and intermediate mechanical performance. Moreover, the films presented high antimicrobial and antioxidant activities in both open and closed systems for up to 15 days. Finally, the food safety of the multilayers was assessed by migration and cytotoxicity tests, demonstrating that the films are safe to use in both alcoholic and acid food simulants and they are also not cytotoxic for Caco-2 cells.


2008 ◽  
Vol 43 (10) ◽  
pp. 1887-1895 ◽  
Author(s):  
Ekambaram Padmini ◽  
Krishnan Prema ◽  
Bose Vijaya Geetha ◽  
Munuswamy Usha Rani

Author(s):  
Andruţa Elena MUREŞAN ◽  
Sevastiţa MUSTE ◽  
Georgiana PETRUŢ ◽  
Romina Alina VLAIC ◽  
Simona Maria MAN ◽  
...  

Tonic wines are true natural elixirs, having the property to fortify the organism. To achieve a complex product and to supplement the antioxidant properties of the wine, the hawthorn fruits were added. They completed the product by maceration, so that the tonic wine got major active substances which have great medicinal value being effective in treating cardiovascular diseases. The content of polyphenols of tonic wine is about six times higher than in simple wine, the hawthorn representing a product rich in polyphenols (510.2 mg GAE/100g). Antioxidant capacity increased when hawthorn fruits were added, as they have an important contribution in terms of new product antioxidant properties. Hawthorn, honey and rosemary added extra minerals in the total dry extract. The relative density increased due to the substances present in hawthorn. The concentration of alcohol slightly increased due to the fermentation which triggers during maceration. The acidity of the new tonic wine developed here was higher, as compared to the simple wine. Higher acidity also contributed to the palatability, the new tonic wine showing a pleasant refreshing taste.


2020 ◽  
pp. 5-18
Author(s):  
Mikhail Yur'yevich Akimov ◽  
Irina Vasil'yevna Luk’yanchuk ◽  
Ekaterina Viktorovna Zhbanova ◽  
Aleksandr Sergeyevich Lyzhin

For the last time a lot of attention has been paid to strawberry as a garden crop. All this thanks to its preventive and medical benefits related to the consumption of its fruit. The paper presents the data analysis of foreign and domestic researchers on the accumulation of basic food components (sugars, organic acids), vitamins, mineral substances and other valuable ones in. Which determine the high antioxidant properties of strawberries. On the basis of scientific literature sources, the vitamin profile was compiled wich reflects the accumulation of vitamin groups, specific for a given crop. With all diversity of vitamins found in strawberry fruits, the fruits of this plant are primarily good as a valuable source of ascorbic acid, folic acid, polyphenolic compounds (anthocyanins, ellagic acid). As for mineral substances, the presence of iron amount confirms the fact. In spite of fact that the strawberry composition is studied enough, the further detailed characterization of genetic resources is necessary both by total antioxidant activity and by definite chemical components, the last ones, obviously, present the antioxidant complex of strawberry fruits. The given information will be a base for future related investigations of phytochemical compounds of fruits and berries. They are extraordinary important in healthy food of humans as well as in creation of nutraceutical products.


2019 ◽  
Vol 6 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Aleksandra Sentkowska ◽  
Krystyna Pyrzynska

Introduction: The antioxidant interactions between the commonly used pharmaceuticals (diclofenac, ibuprofen and naproxen) and green tea polyphenols were evaluated. Methods: The antioxidant properties of the mixtures were evaluated by a scavenging effect on the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical. Results: The mixtures contained green tea extract and each drug exhibited lower antioxidant activity than the mathematical sum of the results obtained for individual components showing antagonistic effect. The results were obtained using isobolographic analysis and interaction factors also suggested the antagonistic type of interaction. Only when the concentration of the green tea infusion was relatively high (in comparison to the drug), an additive effect could be concluded. Conclusion: The high concentration of green tea infusion in comparison to the drug should be used in developing the new formulations as it can help in the therapy due to their antioxidant properties.


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