scholarly journals Optimizing the Efficiency of a Cytocompatible Carbon-Dots-Based FRET Platform and Its Application as a RiboflavinSensor in Beverages

Nanomaterials ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1981
Author(s):  
Roberto Sotolongo-García ◽  
Eustolia Rodríguez-Velázquez ◽  
Manuel Alatorre-Meda ◽  
Mercedes T. Oropeza-Guzmán ◽  
Antonio Tirado-Guízar ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Carbon Dots ◽  
Fluorescent Sensor ◽  
Low Cost ◽  
Resonance Energy ◽  
Standard Addition ◽  
Water Soluble ◽  
Process Conditions ◽  
Intracellular Sensing

In this work, the Förster resonance energy transfer (FRET) between carbon dots (CDs) as energy donors and riboflavin (RF) as an energy acceptor was optimized and the main parameters that characterize the FRET process were determined. The results were successfully applied in the development of an ultrasensitive ratiometric fluorescent sensor for the selective and sensitive determination of RF in different beverages. Water-soluble CDs with a high quantum yield (54%) were synthesized by a facile and direct microwave-assisted technique. The CDs were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), Zeta potential, and UV-visible and molecular fluorescence spectroscopy. The study of the FRET process at two donor concentrations showed that the energy transfer efficiency decreases as the donor concentration increases, confirming its dependence on the acceptor:donor ratio in nanoparticle-based systems. The results show the importance of optimizing the FRET process conditions to improve the corresponding output signal. The variation in the ratiometric signal with the concentration of RF showed linearity in a concentration range of 0 to 11 µM with R2 = 0.9973 and a detection limit of 0.025 µM. The developed nanosensor showed good selectivity over other possible types of interference. The sensor was then applied for the determination of RF in beverage samples using the standard addition method with recoveries between 96% and 106%. Preliminary cytocompatibility tests carried out with breast cancer cells (MDA-MB-231) revealed the nanosensor to be cytocompatible in its working concentration regime, even after long incubation times with cells. Altogether, the developed RF determination method was found to be fast, low-cost, highly sensitive, and selective and can be extended to other samples of interest in the biological and food sectors. Moreover, thanks to its long-lasting cytocompatibility, the developed platform can also be envisaged for other applications of biological interest, such as intracellular sensing and staining for live cell microscopy.

Facile synthesis of fluorescent carbon dots for determination of curcumin based on fluorescence resonance energy transfer

RSC Advances ◽  
2015 ◽  
Vol 5 (79) ◽  
pp. 64790-64796 ◽  
Author(s):  
Ying Shi ◽  
Chunyan Li ◽  
Shaopu Liu ◽  
Zhongfang Liu ◽  
Jinghui Zhu ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Carbon Dots ◽  
Fluorescence Probe ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Facile Synthesis ◽  
Fluorescence Resonance ◽  
Fluorescent Carbon Dots

A carbon dot-based fluorescence probe was designed for detecting curcumin via fluorescence resonance energy transfer.

A chemiluminescence resonance energy transfer for the determination of indolyl acetic acid using luminescent nitrogen-doped carbon dots as acceptors

2018 ◽  
Vol 42 (1) ◽  
pp. 388-394 ◽  
Author(s):  
Yan Liu ◽  
Suqin Han
Keyword(s):  
Acetic Acid ◽  
Energy Transfer ◽  
Carbon Dots ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Nitrogen Doped ◽  
Indolyl Acetic Acid ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

A CRET method was fabricated for the determination of IAA using Ce(iv)–Na2SO3 as the donor and N-CDs as the acceptor.

Tuning Optical Properties of Nitrogen-Doped Carbon Dots Through Fluorescence Resonance Energy Transfer Using Rhodamine B for Ratiometric Sensing of Mercury ion

2021 ◽  
Author(s):  
Alagan Muthurasu ◽  
V GANESH
Keyword(s):  
Optical Properties ◽  
Energy Transfer ◽  
Rhodamine B ◽  
Carbon Dots ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Nitrogen Doped ◽  
Physical Chemical ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

Carbon dots (CDs) exhibiting fluorescence property are generally derived from carbonaceous materials and possessing ultra small size with various exciting physical, chemical and photo-properties that have been used in many...

scholarly journals Synthesis of Fluorescent Carbon Dots and Their Application in Ascorbic Acid Detection

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1246
Author(s):  
Tengfei Wang ◽  
Hui Luo ◽  
Xu Jing ◽  
Jiali Yang ◽  
Meijun Huo ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Fluorescence Intensity ◽  
Carbon Dots ◽  
Ph Dependence ◽  
Scale Up ◽  
Water Soluble ◽  
Jujube Fruit ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Water-soluble fluorescent carbon dots (CDs) were synthesized by a hydrothermal method using citric acid as the carbon source and ethylenediamine as the nitrogen source. The repeated and scale-up synthetic experiments were carried out to explore the feasibility of macroscopic preparation of CDs. The CDs/Fe3+ composite was prepared by the interaction of the CDs solution and Fe3+ solution. The optical properties, pH dependence and stability behavior of CDs or the CDs/Fe3+ composite were studied by ultraviolet spectroscopy and fluorescence spectroscopy. Following the principles of fluorescence quenching after the addition of Fe3+ and then the fluorescence recovery after the addition of asorbic acid, the fluorescence intensity of the carbon dots was measured at λex = 360 nm, λem = 460 nm. The content of ascorbic acid was calculated by quantitative analysis of the changing fluorescence intensity. The CDs/Fe3+ composite was applied to the determination of different active molecules, and it was found that the composite had specific recognition of ascorbic acid and showed an excellent linear relationship in 5.0–350.0 μmol·L−1. Moreover, the detection limit was 3.11 μmol·L−1. Satisfactory results were achieved when the method was applied to the ascorbic acid determination in jujube fruit. The fluorescent carbon dots composites prepared in this study may have broad application prospects in a rapid, sensitive and trace determination of ascorbic acid content during food processing.

scholarly journals Structural Determination of Lipid-bound ApoA-I Using Fluorescence Resonance Energy Transfer

2000 ◽  
Vol 275 (47) ◽  
pp. 37048-37054 ◽  
Author(s):  
Hui-hua Li ◽  
Douglas S. Lyles ◽  
Michael J. Thomas ◽  
Wei Pan ◽  
Mary G. Sorci-Thomas
Keyword(s):  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Structural Determination ◽  
Fluorescence Resonance

Fluorescence resonance energy transfer monitoring of pH-responsive doxorubicin release from carbon dots/aptamer functionalized magnetic mesoporous silica

Nanomedicine ◽  
2021 ◽  
Author(s):  
Abolghasem Abbasi Kajani ◽  
Masoud Ayatollahi Mehrgardi
Keyword(s):  
Drug Delivery ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Carbon Dots ◽  
Drug Loading ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Spectroscopic Techniques ◽  
Ph Responsive ◽  
Fluorescence Resonance

Aim: To develop a novel theranostic nanoplatform for simultaneous fluorescent monitoring and stimuli-triggered drug delivery. Materials & methods: Different microscopic and spectroscopic techniques were used for the characterization of nanocarriers. MCF-7 and human umbilical vein endothelial cell lines were cultured and treated with different doses of doxorubicin-loaded nanocarriers. The cell viability and drug release were studied using MTT assay and fluorescence microscopy. Results: Biocompatible and mono-disperse nanocarriers represent hollow and mesoporous structures with the calculated surface area of 552.83 m2.g-1, high magnetic activity (12.6 emu.g-1), appropriate colloidal stability and high drug loading capacity (up to 61%). Conclusion: Taxane-based carbon dots act as the pH-responsive gatekeepers for the controlled release of doxorubicin into cancer cells and provide a fluorescence resonance energy transfer system for real-time monitoring of drug delivery.

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