scholarly journals Chlortetracycline-Functionalized Silver Nanoparticles as a Colorimetric Probe for Aminoglycosides: Ultrasensitive Determination of Kanamycin and Streptomycin

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 997 ◽  
Author(s):  
Ganesh Dattatraya Saratale ◽  
Rijuta Ganesh Saratale ◽  
Gajanan Ghodake ◽  
Surendra Shinde ◽  
Dae-Young Kim ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Color Change ◽  
Limit Of Detection ◽  
Colorimetric Assay ◽  
Uv Spectroscopy ◽  
Aminoglycoside Antibiotics ◽  
Sensing Element ◽  
Colorimetric Probe ◽  
Aqueous Conditions

Aminoglycosides (AMGs) have been extensively used to treat infectious diseases caused by Gram-negative bacteria in livestock and humans. A selective and sensitive colorimetric probe for the determination of streptomycin and kanamycin was proposed based on chlortetracycline-coated silver nanoparticles (AgNPs–CTC) as the sensing element. Almost all of the tested aminoglycoside antibiotics can rapidly induce the aggregation of AgNPs, along with a color change from yellow to orange/red. The selective detection of aminoglycoside antibiotics, including tobramycin, streptomycin, amikacin, gentamicin, neomycin, and kanamycin, with other types of antibiotics, can be achieved by ultraviolet (UV) spectroscopy. This developed colorimetric assay has ability to detect various AMGs using in-depth surface plasmon resonance (SPR) studies. With this determination of streptomycin and kanamycin was achieved at the picomolar level (pM) by using a UV–visible spectrophotometer. Under aqueous conditions, the linear range of the colorimetric sensor for streptomycin and kanamycin was 1000–1,1000 and 120–480 pM, respectively. The corresponding limit of detection was 2000 pM and 120 pM, respectively. Thus, the validated dual colorimetric and ratiometric method can find various analytical applications for the ultrasensitive and rapid detection of AMG antibiotics in water samples.

DETERMINATION OF AMINOGLYCOSIDE ANTIBIOTICS BY A COLORIMETRIC METHOD BASED ON THE AGGREGATION OF GOLD NANOPARTICLES

NANO ◽  
2013 ◽  
Vol 08 (04) ◽  
pp. 1350037 ◽  
Author(s):  
RUIYONG WANG ◽  
SHUMIN FAN ◽  
RUIQIANG WANG ◽  
RUI WANG ◽  
HUANJING DOU ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Incubation Time ◽  
Color Change ◽  
Limit Of Detection ◽  
Colorimetric Method ◽  
Aminoglycoside Antibiotics ◽  
Sensing Element ◽  
Experimental Conditions ◽  
Experimental Parameters

A sensitive and selective colorimetric biosensor for determination of gentamicin, amikacin and tobramycin was proposed with the unmodified gold nanoparticles (GNPs) as the sensing element. Gentamicin, amikacin and tobramycin can rapidly induce the aggregation of gold nanoparticles and is accompanied by a color change from red to blue. The concentration of gentamicin, amikacin and tobramycin can be determined by using UV-Vis spectrometer. The experimental parameters were optimized with regard to pH, incubation time and the concentration of the GNPs. Under optimal experimental conditions, the linear range of the colorimetric sensor for gentamicin/amikacin/tobramycin were 2.67–33.93 ng mL-1, 13.33–66.67 ng mL-1 and 20–180 ng mL-1, respectively. The corresponding limit of detection (3σ) was 0.354 ng mL-1, 0.999 ng mL-1 and 0.579 ng mL-1, respectively. This assay was simple and used to detect aminoglycoside antibiotics in milk and medicine products.

Silver Nanoparticles as a New Colorimetric Probe for Determination of Oxalic Acid in Urine

2016 ◽  
Vol 14 (9) ◽  
pp. 906-912 ◽  
Author(s):  
Parisa Fateh Basharzad ◽  
Khalil Farhadi ◽  
Mehrdad Forough ◽  
Rahim Molaei
Keyword(s):  
Silver Nanoparticles ◽  
Oxalic Acid ◽  
Colorimetric Probe

scholarly journals Label-Free Colorimetric Detection of Urine Glucose Based on Color Fading Using Smartphone Ambient-Light Sensor

Chemosensors ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 10 ◽  
Author(s):  
Wang ◽  
Guo ◽  
Hu ◽  
Liang ◽  
Li ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Colorimetric Assay ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Diabetic Patients ◽  
Ambient Light ◽  
Label Free ◽  
Urine Glucose ◽  
Light Sensor

In this work, a label-free colorimetric assay was developed for the determination of urine glucose using smartphone ambient-light sensor (ALS). Using horseradish peroxidase—hydrogen peroxide—3,3′,5,5′-tetramethylbenzidine (HRP-H2O2-TMB) colored system, quantitative H2O2 was added to samples to-be-determined for deepest color. The presence of glucose oxidase in urine led to the formation of H2O2 and the reduction of TMBred. As a result of this, the color of the urine faded and the solution changed from deep blue to light blue. We measured the illuminance of the transmitted light by a smartphone ambient light sensor, and thereby color changes were used to calculate the content of urine glucose. After method validation, this colorimetric assay was practically applied for the determination of urine samples from diabetic patients. Good linearity was obtained in the range of 0.039–10.000 mg/mL (R2 = 0.998), and a limit of detection was 0.005 mg/mL. Our method was had high accuracy, sensitivity, simplicity, rapidity, and visualization, providing a new sensor to be potentially applicable for point-of-care detection of urine glucose.

scholarly journals A novel optode sensor for the determination of palladium(II) in water and a hydrogenation catalyst

2009 ◽  
Vol 74 (3) ◽  
pp. 311-315 ◽  
Author(s):  
Hossein Tavallali ◽  
Pisheh Jahromi
Keyword(s):  
Response Time ◽  
Aqueous Solutions ◽  
Optical Sensor ◽  
Color Change ◽  
Limit Of Detection ◽  
Linear Range ◽  
Hydrogenation Catalyst ◽  
Catalyst Sample ◽  
Dynamic Linear Range

A novel optical sensor was established to determine palladium(II) based on the immobilization of 1-(2-pyridylazo)-2-naphthol (PAN) on a triacetylcellulose membrane. Palladium ions react with the immobilized PAN and cause a decrease in the absorbance of the membrane at 469 nm. The response time of the optode was 8-10 min depending on the concentration of Pd(II) ions. This sensing phase had a dynamic linear range of 0.10-12.0 ?g ml-1 palladium ions with a limit of detection of 65 ng ml-1. The sensor can readily be regenerated using an ethylenediamine solution. The sensor could be fully regenerated, and the color change was fully reversible. The method was successfully applied for the determination of Pd(II) in synthetic aqueous solutions and in a hydrogenation catalyst sample.

Development and Validation of UV-Spectrophotometric Methods for the Determination of Berberine in Polymeric Nanoparticles

2019 ◽  
Vol 11 (12) ◽  
pp. 1273-1278
Author(s):  
Md Ali Mujtaba
Keyword(s):  
Phosphate Buffer ◽  
Limit Of Detection ◽  
Polymeric Nanoparticles ◽  
Uv Spectroscopy ◽  
Pharmaceutical Formulation ◽  
Limit Of Quantification ◽  
Spectrophotometric Methods ◽  
Relative Standard ◽  
Development And Validation

A simple, specific, economic, accurate, and reproducible UV-spectrophotometric methods were developed and validated for the estimation of berberine (BRC) in bulk and pharmaceutical formulation. The λmax of BRC in 0.1 N hydrochloric acid (pH 1.2), phosphate buffer (pH 6.8), and water was found to be 346 nm, 343 nm and 260 nm respectively. Beer's law was obeyed in the concentration range of 5–30 μg/ml (R2 = 0.9698) in water, 5–25 μg/ml (R2 = 0.9991) in 0.1 N HCl buffer (pH 1.2) and 5–35 μg/ml (R2 = 0.9935) in phosphate buffer (pH 6.8). These methods were tested, and validated for various parameters such as linearity, precision, accuracy, specificity, limit of detection (LOD), and limit of quantification (LOQ) according to ICH guidelines. The method showed good reproducibility and recovery with percent relative standard deviation less than 2%. Moreover, the accuracy and precision obtained implied that UV spectroscopy can be a cheap, reliable, and less time consuming alternative for chromatographic analysis. The proposed methods were successfully applied for the determination of BRC in pharmaceutical formulation. The BRC estimated from the formulation was found to be well within limits (±5% of the labelled content of the formulations). The proposed methods are highly sensitive, precise, accurate, and can be employed for the routine analysis of berberine in bulks as well as in the commercial formulations.

scholarly journals Highly Sensitive and Selective Colorimetric Detection of Creatinine Based on Synergistic Effect of PEG/Hg2+–AuNPs

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1424 ◽  
Author(s):  
Xia ◽  
Zhu ◽  
Bian ◽  
Li ◽  
Liu ◽  
...  
Keyword(s):  
Color Change ◽  
Limit Of Detection ◽  
Colorimetric Detection ◽  
High Sensitivity ◽  
Peak Shift ◽  
Creatinine Concentration ◽  
Highly Sensitive ◽  
Coordination Effect ◽  
Bovine Serum Sample

A colorimetric sensor, based on the synergistic coordination effect on a gold nanoparticle (AuNP) platform has been developed for the determination of creatinine. The sensor selects citrate stabilized AuNPs as a platform, polyethylene glycol (PEG) as a decorator, and Hg2+ as a linkage to form a colorimetric probe system (PEG/Hg2−–AuNPs). By forming hydrogen bond between the oxygen-containing functional groups of PEG and citrate ions on the surface of AuNPs, this probe shows good stability. PEG coordinated with Hg2+ synergistically and specifically on the surface of dispersed AuNPs, and the existence of creatinine could induce the aggregation of AuNPs with a corresponding color change and an obvious absorption peak shift within 5 min. This PEG/Hg2+–AuNPs probe towards creatinine shows high sensitivity, and a good linear relationship (R2 = 0.9948) was obtained between A620–522 nm and creatinine concentration, which can achieve the quantitative calculations of creatinine. The limit of detection (LOD) of this PEG/Hg2+–AuNPs probe was estimated to be 9.68 nM, lower than that of many other reported methods (Supplementary Materials Table S3). Importantly, the sensitive probe can be successfully applied in a urine simulating fluid sample and a bovine serum sample. The unique synergistic coordination sensing mechanism applied in the designation of this probe further improves its high selectivity and specificity for the detection of creatinine. Thus, the proposed probe may give new inspirations for colorimetric detection of creatinine and other biomolecules.

scholarly journals Determination of Lipoxygenase Activity in Cereals Grains Using a Silver Nanoparticles Assay

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Raybel Muñoz ◽  
Jose A. Rodriguez ◽  
M. Elena Páez-Hernández ◽  
Irais Sánchez-Ortega ◽  
Eva M. Santos
Keyword(s):  
Silver Nanoparticles ◽  
Enzyme Activity ◽  
Limit Of Detection ◽  
Xylenol Orange ◽  
Enzymatic Reaction ◽  
Lipoxygenase Activity ◽  
Cereal Grains ◽  
Optimal Conditions ◽  
Relative Standard

A spectrophotometric method based on the use of silver nanoparticles is presented for the determination of lipoxygenase activity in cereal grains. The method involves the synthesis of prism silver nanoparticles with a maximum absorption at 752 nm, followed by the abatement of the signal as a consequence of the oxidation produced by hydroperoxides generated in the enzymatic reaction. The quantification of the hydroperoxides produced by reaction of lipoxygenase with linoleic acid allows the determination of the enzyme activity in cereal grains. Under optimal conditions, the linear range of the calibration curve ranges from 1.0 to 5.0 μM, with a limit of detection of 0.34 μM. The method was validated comparing the results with those obtained by ferrous-xylenol orange method. A relative standard deviation < 5.0% was obtained in all cases and no significant differences were observed (p<0.05).

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