scholarly journals Internalization and Viability Studies of Suspended Nanowire Silicon Chips in HeLa Cells

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 893 ◽  
Author(s):  
Sara Durán ◽  
Marta Duch ◽  
Rodrigo Gómez-Martínez ◽  
Marta Fernández-Regúlez ◽  
Juan Pablo Agusil ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Hela Cells ◽  
Silicon Nanowires ◽  
Drug Delivery Systems ◽  
Silicon Nanowire ◽  
Membrane Vesicles ◽  
Delivery Systems ◽  
Fundamental Study ◽  
Silicon Chips ◽  
Functional Features

Micrometer-sized silicon chips have been demonstrated to be cell-internalizable, offering the possibility of introducing in cells even smaller nanoelements for intracellular applications. On the other hand, silicon nanowires on extracellular devices have been widely studied as biosensors or drug delivery systems. Here, we propose the integration of silicon nanowires on cell-internalizable chips in order to combine the functional features of both approaches for advanced intracellular applications. As an initial fundamental study, the cellular uptake in HeLa cells of silicon 3 µm × 3 µm nanowire-based chips with two different morphologies was investigated, and the results were compared with those of non-nanostructured silicon chips. Chip internalization without affecting cell viability was achieved in all cases; however, important cell behavior differences were observed. In particular, the first stage of cell internalization was favored by silicon nanowire interfaces with respect to bulk silicon. In addition, chips were found inside membrane vesicles, and some nanowires seemed to penetrate the cytosol, which opens the door to the development of silicon nanowire chips as future intracellular sensors and drug delivery systems.

scholarly journals Advances in plant-derived edible nanoparticle-based lipid nano-drug delivery systems as therapeutic nanomedicines

2018 ◽  
Vol 6 (9) ◽  
pp. 1312-1321 ◽  
Author(s):  
Chunhua Yang ◽  
Mingzhen Zhang ◽  
Didier Merlin
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Membrane Vesicles ◽  
Delivery Systems ◽  
Edible Plants ◽  
Nano Drug Delivery

Plant-derived edible nanoparticles (PDNPs) are nano-sized membrane vesicles released by edible plants, such as grapefruit, ginger, broccoli, and lemon.

scholarly journals The Enhanced Inhibitory Effect of Different Antitumor Agents in Self-Microemulsifying Drug Delivery Systems on Human Cervical Cancer HeLa Cells

Molecules ◽  
2015 ◽  
Vol 20 (7) ◽  
pp. 13226-13239 ◽  
Author(s):  
Zoltán Ujhelyi ◽  
Azin Kalantari ◽  
Miklós Vecsernyés ◽  
Eszter Róka ◽  
Ferenc Fenyvesi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cervical Cancer ◽  
Hela Cells ◽  
Drug Delivery Systems ◽  
Antitumor Agents ◽  
Inhibitory Effect ◽  
Delivery Systems ◽  
Human Cervical Cancer

scholarly journals Engineered Remolding and Application of Bacterial Membrane Vesicles

2021 ◽  
Vol 12 ◽  
Author(s):  
Li Qiao ◽  
Yifan Rao ◽  
Keting Zhu ◽  
Xiancai Rao ◽  
Renjie Zhou
Keyword(s):  
Drug Delivery ◽  
Bacterial Infections ◽  
Drug Delivery Systems ◽  
Membrane Vesicles ◽  
Delivery Systems ◽  
Bacterial Membrane ◽  
Wild Type ◽  
Bacterial Strains

Bacterial membrane vesicles (MVs) are produced by both Gram-positive and Gram-negative bacteria during growth in vitro and in vivo. MVs are nanoscale vesicular structures with diameters ranging from 20 to 400 nm. MVs incorporate bacterial lipids, proteins, and often nucleic acids, and can effectively stimulate host immune response against bacterial infections. As vaccine candidates and drug delivery systems, MVs possess high biosafety owing to the lack of self-replication ability. However, wild-type bacterial strains have poor MV yield, and MVs from the wild-type strains may be harmful due to the carriage of toxic components, such as lipopolysaccharides, hemolysins, enzymes, etc. In this review, we summarize the genetic modification of vesicle-producing bacteria to reduce MV toxicity, enhance vesicle immunogenicity, and increase vesicle production. The engineered MVs exhibit broad applications in vaccine designs, vaccine delivery vesicles, and drug delivery systems.

scholarly journals Biomimetic Bacterial Membrane Vesicles for Drug Delivery Applications

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1430
Author(s):  
Sajid Fazal ◽  
Ruda Lee
Keyword(s):  
Drug Delivery ◽  
Delivery System ◽  
Mammalian Cells ◽  
Bacterial Infections ◽  
Drug Delivery Systems ◽  
Large Scale ◽  
Scale Up ◽  
Membrane Vesicles ◽  
Delivery Systems ◽  
Bacterial Membrane

Numerous factors need to be considered to develop a nanodrug delivery system that is biocompatible, non-toxic, easy to synthesize, cost-effective, and feasible for scale up over and above their therapeutic efficacy. With regards to this, worldwide, exosomes, which are nano-sized vesicles obtained from mammalian cells, are being explored as a biomimetic drug delivery system that has superior biocompatibility and high translational capability. However, the economics of undertaking large-scale mammalian culture to derive exosomal vesicles for translation seems to be challenging and unfeasible. Recently, Bacterial Membrane Vesicles (BMVs) derived from bacteria are being explored as a viable alternative as biomimetic drug delivery systems that can be manufactured relatively easily at much lower costs at a large scale. Until now, BMVs have been investigated extensively as successful immunomodulating agents, but their capability as drug delivery systems remains to be explored in detail. In this review, the use of BMVs as suitable cargo delivery vehicles is discussed with focus on their use for in vivo treatment of cancer and bacterial infections reported thus far. Additionally, the different types of BMVs, factors affecting their synthesis and different cargo loading techniques used in BMVs are also discussed.

Techniques For The Preparation of Tissue Samples Containing Injectable Polymer Microcapsules

1985 ◽  
Vol 43 ◽  
pp. 710-711
Author(s):  
G.E. Visscher ◽  
R. L. Robison ◽  
G. J. Argentieri
Keyword(s):  
Drug Delivery ◽  
Drug Delivery Systems ◽  
Gastrocnemius Muscle ◽  
Degradation Process ◽  
Delivery Systems ◽  
Tissue Reaction ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Injectable Polymer ◽  
Microscopic Techniques

The use of various bioerodable polymers as drug delivery systems has gained considerable interest in recent years. Among some of the shapes used as delivery systems are films, rods and microcapsules. The work presented here will deal with the techniques we have utilized for the analysis of the tissue reaction to and actual biodegradation of injectable microcapsules. This work has utilized light microscopic (LM), transmission (TEM) and scanning (SEM) electron microscopic techniques. The design of our studies has utilized methodology that would; 1. best characterize the actual degradation process without artifacts introduced by fixation procedures and 2. allow for reproducible results.In our studies, the gastrocnemius muscle of the rat was chosen as the injection site. Prior to the injection of microcapsules the skin above the sites was shaved and tattooed for later recognition and recovery. 1.0 cc syringes were loaded with the desired quantity of microcapsules and the vehicle (0.5% hydroxypropylmethycellulose) drawn up. The syringes were agitated to suspend the microcapsules in the injection vehicle.

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