scholarly journals Proximate Composition, Bioactive Compounds, and Antioxidant Potential of Wild Halophytes Grown in Coastal Salt Marsh Habitats

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 28
Author(s):  
Yasser A. El-Amier ◽  
Walid Soufan ◽  
Khalid F. Almutairi ◽  
Nouf S. Zaghloul ◽  
Ahmed M. Abd-ElGawad

Halophytes have been characterized as a potential resource for fiber, food, fodder, and bioactive compounds. Proximate composition, bioactive compounds, and antioxidant activity of five wild dominant halophytes (Arthrocnemummacrostachyum, Halocnemumstrobilaceum, Limoniastrummonopetalum, Limoniastrumpruinosum, and Tamarix nilotica) naturally growing along the Nile Delta coast were assessed. The soil supporting these halophytes was sandy to sand-silty, alkaline, with low organic carbon, and relatively high CaCO3. H. strobilaceum attained the highest moisture content, ash, crude fiber, lipids, and total soluble sugars. L. monopetalum showed the highest content of crude protein (18.00%), while T. nilotica had the highest content of total carbohydrates. The studied halophytes can be ranked according to their nutritive value as follows: H.strobilaceum > L.monopetalum > A.macrostachyum > L.pruinosum > T. nilotica. A. macrostachyum attained the highest amount of Na+, K+, Ca2+, and Mg2+. A. macrostachyum showed a high content of phenolic compounds, while H.strobilaceum was rich in tannins and saponin contents. The MeOH extract of A. macrostachyum and H. strobilaceum exhibited substantial antioxidant activity. The present results showed that the studied halophytes could be considered as candidates for forage production or used as green eco-friendly natural resources for bioactive compounds.

2020 ◽  
Vol 15 (6) ◽  
pp. 637-647
Author(s):  
Priscila do C.M.R. Torma ◽  
Patric de L. Monteiro ◽  
Ana V. Carvalho ◽  
Simone H. Flôres ◽  
Paula R. Augusti ◽  
...  

Background: Açaí (Euterpe oleracea), a “superfruit” consumed worldwide, is a Brazilian Amazon native fruit, with high nutritive value due to its high content of lipids, proteins, and fibers, besides bioactive compounds, such as anthocyanins, carotenoids and phenolic compounds in its composition associated with biological action beneficial to health. The increased consumption of this fruit has stimulated breeding programs in the search of plants and fruits with higher productivity and bioactive compounds, generating information for the development of new cultivars with improved attributes. This work aimed to study six different genotypes of açaí, developed by the breeding program, evaluating their physicochemical characteristics and proximate composition, bioactive compounds and antioxidant activity. Methods: The genotypes were analyzed for titratable acidity, pH, total soluble solids and proximate composition, such as moisture, protein, lipids, total fiber, carbohydrates and ashes contents. A spectrophotometric method using Folin-Ciocalteau reagent performed the phenolic compounds analysis and HPLC evaluated carotenoids and anthocyanins extracted exhaustively. The ABTS assay evaluated the antioxidant activity. Results: The L06P13 and L09P09 genotype presented higher content of total fiber, carbohydrates, and ashes when compared to the commercial sample used as a standard. Furthermore, L22P13 genotype showed the highest content of total anthocyanins (6745.81 mg/100g), total carotenoids (118 μg/g) and antioxidant activity (674.83 μM Trolox/g) when compared to commercial sample. Conclusion: The results revealed promising açaí genotypes and support the importance of advances in the area of functional foods and breeding programs.


2021 ◽  
Vol 11 (16) ◽  
pp. 7638
Author(s):  
Magdalena Ziobroń ◽  
Aneta Kopeć ◽  
Joanna Skoczylas ◽  
Kinga Dziadek ◽  
Jerzy Zawistowski

The aim of this study was to determine the basic chemical composition, the content of bioactive compounds and antioxidant activity in currant leaves. The leaves of black, red and white currant shrubs were collected in May, and in the beginning of June, July and August, for two years between 2018 and 2019. The proximate analysis, including dry matter, protein, fat, ash and total carbohydrates, was determined. In addition, the content of the polyphenols and the total antioxidant activity using ABTS, DPPH and FRAP assays were conducted. The highest concentration of protein was detected in the whitecurrant leaves harvested in May in both years, while the highest content of crude fat was found in the blackcurrant leaves harvested in both years, with the exception of the August harvest. Extracts from the blackcurrant leaves collected in June/July 2019 had the highest antioxidant activity that was measured by the ABTS method (about 7000 µmol Trolox/g DM) and confirmed by other methods, while extracts from the whitecurrant leaves produced from the August 2018 collection had the lowest antioxidant activity (1884 µmol Trolox/g DM). Currant leaves are a rich source of bioactive compounds and contain higher amounts of polyphenols as compared with currant fruits. These compounds may play a very important role in the risk reduction and even prevention of the most chronic non-communicable diseases. Therefore, further research is needed to identify currant leaves as a source of bioactives for functional foods and natural health products. The highest antioxidant activity was in the redcurrant leaves from all the harvest times in 2018 as measured by the ABTS and FRAP methods. On the contrary, blackcurrant leaves from all the harvest times in 2019 had the highest antioxidant activity.


Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 569
Author(s):  
Saud L. Al-Rowaily ◽  
Ahmed M. Abd-ElGawad ◽  
Suliman M. Alghanem ◽  
Wafa’a A. Al-Taisan ◽  
Yasser A. El-Amier

Geophytes are plants with underground storage organs including bulbs, corms, tubers, and rhizomes, often physiologically active and able to survive during harsh environmental conditions. This study is conducted to assess the nutritive value, mineral composition, bioactive metabolites, and antioxidant activity of five wild geophytes (Cyperus capitatus, C. conglomeratus, Elymus farctus, Lasiurus scindicus, and Panicum turgidum) collected from the Nile Delta coast and inland desert. The proximate composition including dry matter, moisture content, ash content, fiber, fat, protein, sucrose, and glucose were determined. Also, total carbohydrates, total digestible nutrients (TDN), and nutritive values were calculated. Macro- and micro-minerals were also determined in the studied geophytes. Total phenolics, total flavonoids, alkaloids, saponins, and tannins were determined. Antioxidant activity was evaluated based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicle scavenging. Based on the nutritive value, the studied geophytes are ranked as follows: E. farctus > C. conglomeratus > L. scindicus > P. turgidum > C. capitatus. The mineral analysis reveals a sufficient amount of macro- and micro-elements in the studied geophytes while the microelements levels in the studied wild plants exist as Fe > Mn > Zn > Cu. Cyperus conglomeratus attained the highest concentrations of all determined secondary metabolites. On the other hand, C. conglomeratus, C. capitatus, and P. turgidum extracts showed strong scavenging activity (EC50 < 1 mg mL−1), while extracts of E. farctus and L. scindicus exhibited moderate scavenging activity (1 ≤ EC50 ≤ 2 mg mL−1). The present data reveal that geophytes under investigation could be used as good forage plants, especially in arid habitats. In addition, C. conglomeratus could be a potentially important candidate for natural antioxidants as it attained high contents of the bioactive constituents.


Foods ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 642
Author(s):  
Laura Cebadera ◽  
Maria Inês Dias ◽  
Lillian Barros ◽  
Virginia Fernández-Ruiz ◽  
Rosa Mª Cámara ◽  
...  

The most relevant nutrients and bioactive compounds (soluble sugars, dietary fiber, ascorbic acid and organic acids, individual phenolic compounds, fatty acids, and tocopherols) as well as antioxidant activity have been characterized in three extra early varieties of clementine (Citrus clementina Hort ex Tan. Basol, Clemensoon and Clemenrubí) cultivated in Valencia (Spain). Clementines are a relevant source of bioactive compounds, such as vitamin C (values around 80 mg/100 g), allowing to satisfy the recommended daily intake with the consumption of a normal portion. Sucrose was the most abundant sugar, and potassium the main mineral while manganese was the least. Fat content was very low (<0.5 mg/100 g), with palmitic acid and α-tocopherol the most abundant fatty acid and vitamin E form, respectively. Flavonoids were the predominant phenolic compounds, with narirutin/naringin and (neo)hesperidin the best represented ones. The antioxidant capacity evaluated by reducing power, DPPH, and β-carotene bleaching inhibition assays was satisfactory with values similar to those reported in other citrus fruits. Thus, this fruit is a relevant source of bioactive compounds with antioxidant properties of interest for consumers and the food industry.


2018 ◽  
Vol 47 (1) ◽  
pp. 81-91 ◽  
Author(s):  
Elżbieta JĘDRSZCZYK ◽  
Aneta KOPEĆ ◽  
Piotr BUCKI ◽  
Anna M. AMBROSZCZYK ◽  
Barbara SKOWERA

The aim of the study was to evaluate the effect of biostimulants enhancing plant growth and development, i.e. organic stimulator based on metal sulphates, Bacillus subtilis, humic acids, on the chemical composition and level of bioactive compounds in garlic leaves, stems, and bulbs. The study was conducted in three growing seasons on the ‘Ornak’ winter garlic cultivar. The propagating material was treated in solutions of the biostimulants and after emergence the plants were sprayed with the products three times. The most valuable edible organ proved to be the leaves, which contained the most protein, fat, and mineral components expressed as ash, were the richest source of fibre, vitamin C, and polyphenols, and also had the highest antioxidant activity among the organs tested. The nutritional value of the stems was comparable to that of the bulbs, and the level of polyphenols and antioxidant activity were even higher. The use of the growth and development biostimulants influenced the chemical composition of the garlic, but the effect was modified by the course of weather in different years of the research and also depended on edible organ. On average for years and organs all biostimulants increased the level of protein, minerals expressed as ash and enhanced antioxidant activity, whereas decreased the level of total carbohydrates.


2019 ◽  
Vol 72 (1) ◽  
Author(s):  
Rita Jurkow ◽  
Agata Wurst ◽  
Andrzej Kalisz ◽  
Agnieszka Sękara ◽  
Stanisław Cebula

Kale is a plant known and valued since antiquity as a healthy vegetable crop, used for culinary, decorative, but also healing purposes. The aim of the study was to examine the effect of harvest date on physiological status and nutritional composition of two kale cultivars: ‘Winterbor’ F<sub>1</sub> (blue-green leaves) and ‘Redbor’ F<sub>1</sub> (red-purple leaves). The leaves were harvested in three periods: before frost (&gt;0°C), after medium (−5.0°C) and heavy frost (−15.0°C). Content of dry weight, soluble sugars, l-ascorbic acid, carotenoids, chlorophylls, polyphenols, anthocyanins, as well as antioxidant activity and peroxidase activity were determined. Cold temperature significantly affected bioactive compounds of kale. The content of dry weight, soluble sugars, l-ascorbic acid, phenolics, and antioxidant activity increased after medium frosts for both cultivars. The level of anthocyanins also increased significantly for the ‘Redbor’ F<sub>1</sub> cultivar. After strong frost, most of the tested parameters (content of dry weight, soluble sugars, phenolics, anthocyanins, and total antioxidant and peroxidase activity) significantly increased. The chlorophyll <em>a</em> content was reduced by heavy frost in both seasons. Harvesting kale before and after frost may allow the level of biologically active ingredients to be regulated as cold also significantly affects the physiological status of the plants.


Author(s):  
Naama Jessica de Assis Melo ◽  
Andréia Mitsa Paiva Negreiros ◽  
José Dárcio Abrantes Sarmento ◽  
Patrícia Lígia Dantas de Morais ◽  
Rui Sales Júnior

Grafting is widely used to provide resistance to pests and diseases in yellow passion fruit, such as fusariosis, caused by the fungus Fusarium oxysporum f. sp. passiflorae (FOP). However, this practice may generate some changes in the postharvest characteristics of the fruits. The objective of this work was to verify the postharvest quality of fruits of P. edulis collected mature and ripe in three areas with different forms of cultivation (conventional without grafting, conventional with grafting and organic with grafting). The experiment was installed in a completely randomized design, in a 3 x 2 factorial scheme, with five replicates of four fruits each. The first factor was the different cultivation systems and the second factor was the maturity stage. The fruits were harvested, selected and submitted to physical, physical-chemical and chemical analysis, bioactive compounds and antioxidant activity. Fruit and pulp weight, longitudinal and transverse length and firmness were superior in fruits from conventional without grafting system, however fruit yield was inferior for this system. Soluble solids, pH, reducing sugars and total soluble sugars were superior in fruits from organic with grafting system. Bioactive compounds and antioxidant activity were improved in conventional without grafting system fruits.


Author(s):  
Waras Nurcholis ◽  
Edy Djauhari Purwakusumah ◽  
Mono Rahardjo ◽  
Latifah K. Darusman

Temulawak (Curcuma  xanthorrhizaRoxb.) belongs to the family Zingiberaceae, has been empirically used as herbal medicines. The research was aimed to evaluate three promising lines of Temulawak based on their high bioactive contents (xanthorrhizol and curcuminoid) and its in vitro bioactivity (antioxidant and toxicity), and to obtain information on agrobiophysic environmental condition which produced high bioactive compounds. The xanthorrhizol and curcuminoid contents were measured by HPLC. In vitro antioxidant and toxicity were determined by DPPH (1,1-diphenyl-2-picryl-hydrazyl) method and BSLT (Brine Shrimp Lethality Test). The result showed that promising line A produced the highest yield of bioactive and bioactivity, i.e. 0.157 and 0.056 g plant-1of xanthorrizol and curcuminoid respectively. The IC50 of antioxidant activity was 65.09 mg L-1and LC50of toxicity was 69.05 mg L-1. In this study, Cipenjo had the best temulawak performance than two other locations. According to the agrobiophysic parameters, Cipenjo environmental condition was suitable for temulawak cultivation with temperature 28-34 ºC, rainfall ± 223.97 mm year-1 and sandy clay soil. Keywords: antioxidant, curcuminoid, promising lines, temulawak, xanthorrhizol


2020 ◽  
Vol 50 (3) ◽  
pp. 460-469
Author(s):  
Damir Zyaitdinov ◽  
Alexandr Ewteew ◽  
Anna Bannikova

Introduction. Bioactive compounds are a very popular topic of modern food science, especially when it concerns obtaining polyphenols from cereals. The antiradical, antioxidant, and anti-inflammatory properties of these ingredients allow them to inhibit and prevent coronary, artery, and cardiovascular diseases, as well as several types of cancer. Encapsulation is an effective technology that protects bioactive ingredients during processing and storage. In addition, it also prevents any possible interaction with other food constituents. The research objective was to obtain effective tools of controlled delivery of bioactive compounds. The study featured whey protein as a wall material in combination with maltodextrin to encapsulate the bioactives from oat bran. Study objects and methods. The processed material was oat bran. The technology of its biotransformation was based on ultrasound processing and enzymatic hydrolysis. The antioxidant properties were determined using a coulometer of Expert – 006-antioxidants type (Econix-Expert LLC, Moscow, Russia). Separation and quantitative determination of extract were followed using a Stayer HPLC device (Akvilon, Russia) and a system column Phenomenex Luna 5u C18(2) (250×4.6 mm). The total phenolic content was measured by a modified Folin-Ciocalteu method. To prepare microcapsules, whey protein concentrate (WPC) and maltodextrin (MD) solutions were mixed at ratios 6:4, 4:6, and 5:5. After that, the mixes were treated by ultrasonication and 10% w/w of guar gum solution as double wall material. The encapsulation efficiency (EE) was determined as a ratio of encapsulated phenolic content to total phenolic content. A digestion protocol that simulates conditions of the human gastric and intestinal tract was adapted to investigate the release kinetics of the extracts. Results and discussion. Ferulic acid is the main antioxidant in cereals. Its amount during extraction was consistent with published data: 9.2 mg/mL after ultrasound exposure, 9.0 mg/mL after enzymatic extraction, and 8.6 mg/mL after chemical treatment. The antioxidant activity of the obtained polyphenols was quite high and reached 921 cu/mL. It depended on the concentration of the preparation in the solution and the extraction method. The polyphenols obtained by ultrasonic exposure and enzyme preparations proved to have a more pronounced antioxidant activity. The highest EE (95.28%) was recorded at WPC:MD ratio of 60:40. In vitro enzymatic hydrolysis protocol simulating digestion in the gastrointestinal tract was used to study the effect of capsule structural characteristics on the kinetics of polyphenol release. The percentage of o polyphenols released from capsules ranged from 70% to 83% after two hours of digestion, which confirmed the effectiveness of microencapsulation technology. Conclusion. The research confirmed the possibility of using polyphenols obtained by the biotechnological method from oat bran as functional ingredients. Eventually, they may be used in new functional products with bifidogenic properties. Whey protein can be used to encapsulate polyphenols as the wall material of microcapsules.


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