scholarly journals Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6183
Author(s):  
Ștefan Ciornei ◽  
Dan Drugociu ◽  
Liliana Margareta Ciornei ◽  
Mihai Mareș ◽  
Petru Roşca
Keyword(s):  
Candida Parapsilosis ◽  
Fungal Species ◽  
Degree Of Contamination ◽  
Average Value ◽  
Microbiological Profile ◽  
Fungal Load ◽  
Pig Farms ◽  
Sus Scrofa Domesticus ◽  
Boar Semen ◽  
Fresh Semen

The aim of the study was to establish the complete microbiological profile of boar semen (Sus scrofa domesticus) and to choose the most effective antiseptic measures in order to control and optimize AI reproduction in pig farms. One hundred and one semen samples were collected and analyzed from several pig farms. The microbiological profile of ejaculates was determined by evaluating the degree of contamination of fresh semen and after dilution with specific extenders. The bacterial and fungal load of fresh boar semen recorded an average value of 82.41/0.149 × 103 CFU/mL, while after diluting the ejaculates the contamination value was 0.354/0.140 × 103 CFU/mL. Twenty-four germs (15 bacterial and 9 fungal species) were isolated, the most common being Candida parapsilosis/sake (92%) and Escherichia coli (81.2%). Modification of the sperm collection protocol (HPBC) reduced contamination in raw sperm by 49.85% in bacteria (significant (p < 0.00001) and by 9.67% in fungi (non-significant (p < 0.111491). The load in bacteria and filamentous fungi can be controllable, but not in levuras fungi. Some fluconazole-added extenders (12.5 mg%), ensure fungal aseptization, and even an increase in sperm progressivity (8.39%) for at least a 12 h shelf life after dilution. Validation of sperm aseptization was done by maintaining sow fecundity unchanged after AI (insignificant p > 0.05).

scholarly journals Total Aseptization of Boar Semen, to Increase the Biosecurity of Reproduction in Swine

2021 ◽  
Author(s):  
Ștefan Ciornei ◽  
Dan Drugociu ◽  
Liliana Margareta Ciornei ◽  
Mihai Mareș ◽  
Roșca Petru
Keyword(s):  
Escherichia Coli ◽  
Shelf Life ◽  
Candida Parapsilosis ◽  
Fungal Species ◽  
Average Value ◽  
Microbiological Profile ◽  
Fungal Load ◽  
Pig Farms ◽  
Boar Semen ◽  
Fresh Semen

The aim of the study is to establish the complete microbiological profile of boar semen and to choose the most effective antiseptic measures in order to control and optimize AI reproduction in pigs. More than one hundred semen samples were collected and analyzed from several pig farms. The microbiological profile of ejaculates was determined by determining the degree of con-tamination of fresh semen and after dilution with specific extenders. The bacterial and fungal load of fresh boar semen recorded an average value of 82.41/0.149x103CFU/mL, while after diluting the ejaculates the contamination value was 0.354/0.140 x103CFU/mL. 23 bacterial and fungal species were isolated, the most common being Candida parapsilosis/sake (92%), Escherichia coli (81.2%). Modification of the sperm collection protocol (HPBC) reduced contamination in raw sperm (by 49.85% in bacteria and by 9.67% in fungi). The load in bacteria and filamentous fungi can be con-trollable, but not in levuras fungi. In some Fluconazole-added extenders (12.5mg%), ensures the solution of this problem, and even increase in sperm progressivity (8.39%) for at least a 12-hour shelf life after dilution. The validation of the experiment was done by obtaining the sow fertility rate after AI.

scholarly journals The Effect of Long Shelf Life of Duroc Pig Male Semen Diluted Using Tris-Egg Yolk-Young Coconut Water on The Value of Viability, Abnormality and pH

2021 ◽  
Vol 3 (2) ◽  
pp. 108-120
Author(s):  
Fransiska Luruk Berek ◽  
Agustinus Agung Dethan ◽  
Paulus Klau Tahuk
Keyword(s):  
Shelf Life ◽  
Egg Yolk ◽  
Mean Value ◽  
Coconut Water ◽  
Average Value ◽  
Randomized Design ◽  
Boar Semen ◽  
The Mean ◽  
Completely Randomized Design ◽  
Fresh Semen

This study aims to determine the effect of shelf life of duroc boar semen which was diluted using tris-egg yolk-coconut water thinner on the value of viability, abnormality, and acidity (pH). The semen used is in the form of fresh semen from 2-year-old duroc pigs. Semen was collected by a manual method using artificial broodstock. This research was conducted in November 2020 using an experimental method according to the Completely Randomized Design (CRD) procedure with 4 treatments and 4 replications so that there were 16 experimental units. Each treatment is P0: storage of semen for 0 hours, P1: storage of semen for 24 hours, P2: storage of semen for 36 hours, P3: storage of semen for 48 hours. The results showed that the use of tris-egg yolk-coconut water diluent had a significant effect (P <0.05) on viability, abnormality, and degree of acidity (pH). The mean value of spermatozoa viability was P0: 96%, P1: 93.75%, P2: 84.5%, and treatment P3: 79.5%. The mean value of spermatozoa abnormality in treatment P0: 5.25%, P1: 6.5%, P2: 11.25%, and treatment P3: 14.75%. The average value of the degree of acidity (pH) of semen P0: 8.3 P1: 7.2; P2 7,3 and P3 treatment: 7,25. It can be concluded that the dilution of semen using tris-egg yolk and coconut water can maintain the value of viability, abnormalities of spermatozoa, and degree of acidity (pH).  

scholarly journals Intranasal Vaccine Using P10 Peptide Complexed within Chitosan Polymeric Nanoparticles as Experimental Therapy for Paracoccidioidomycosis in Murine Model

2020 ◽  
Vol 6 (3) ◽  
pp. 160
Author(s):  
Samuel Rodrigues Dos Santos Junior ◽  
Francenya Kelley Lopes da Silva ◽  
Lucas Santos Dias ◽  
Ana Camila Oliveira Souza ◽  
Marcelo Valdemir de Araujo ◽  
...  
Keyword(s):  
Polymeric Nanoparticles ◽  
Paracoccidioides Brasiliensis ◽  
Physicochemical Characteristics ◽  
Fungal Species ◽  
Experimental Therapy ◽  
Medium Size ◽  
Biological Applications ◽  
Dispersion Index ◽  
Fungal Load ◽  
Intranasal Vaccine

Paracoccidioidomycosis (PCM) is a granulomatous fungal disease caused by the dimorphic fungal species of Paracoccidioides, which mainly affects the lungs. Modern strategies for the treatment and/or prevention of PCM are based on a Th1-type immune response, which is important for controlling the disease. One of the most studied candidates for a vaccine is the P10 peptide, derived from the 43 kDa glycoprotein of Paracoccidioides brasiliensis. In order to improve its immune modulatory effect, the P10 peptide was associated with a chitosan-conjugated nanoparticle. The nanoparticles presented 220 nm medium size, poly dispersion index (PDI) below 0.5, zeta potential of +20 mV and encapsulation efficiency around 90%. The nanoparticles’ non-toxicity was verified by hemolytic test and cell viability using murine macrophages. The nanoparticles were stable and presented physicochemical characteristics desirable for biological applications, reducing the fungal load and the usual standard concentration of the peptide from 4 to 20 times.

Effect of different extenders on quality of frozen-thawed boar semen

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
S Frydrychová ◽  
A Lustyková ◽  
E Václavková ◽  
J Lipenský ◽  
M Rozkot
Keyword(s):  
Sperm Motility ◽  
Aspartate Aminotransferase ◽  
Sperm Concentration ◽  
Holding Time ◽  
Holding Period ◽  
Semen Volume ◽  
Significant Difference ◽  
Boar Semen ◽  
Fresh Semen

The objective of this study was to investigate the effect of using different extenders <italic>viz.</italic> Androhep, Safecell Plus and SUS during cryopreservation on quality of frozen-thawed boar semen. Semen volume, sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa, total number of spermatozoa per ejaculate and activity of the enzyme aspartate aminotransferase (AST) were assessed in fresh semen collected from 39 fertile AI boars. Semen from each boar was divided into three portions and diluted 1:1.5 in extender Androhep, Safecell Plus and SUS and keep at 17°C for 15-h holding time before cryopreservation. Then sperm was cryopreserved. Straws were thawed in a water bath at 38°C for 40s and post-thaw sperm motility with AST activity was assessed. Significant difference in post-thaw sperm motility was found between extender Androhep and Safecell Plus (P<0.05). AST activity did not differ significantly between tested extenders (P>0.05). In conclusion, the results of the study indicate that using Safecell Plus extender during holding period before cryopreservation significantly affected post-thaw sperm motility.

scholarly journals Changes in sperm quality and lipid composition during cryopreservation of boar semen

Reproduction ◽  
2001 ◽  
pp. 395-401 ◽  
Author(s):  
S Cerolini ◽  
A Maldjian ◽  
F Pizzi ◽  
TM Gliozzi
Keyword(s):  
Lipid Content ◽  
Free Cholesterol ◽  
Sperm Quality ◽  
Cholesterol Content ◽  
Sperm Viability ◽  
Freezing And Thawing ◽  
Motile Cells ◽  
Boar Semen ◽  
Thawing Procedure ◽  
Fresh Semen

The effect of cryopreservation on boar sperm viability, motility, lipid content and antioxidant enzymatic activities was studied. Three classes of semen were determined according to a cluster analysis on the basis of the proportion of live and dead cells after freezing and thawing. The classes identified were: high (H, n = 4), average (A, n = 12) and low (L, n = 3) viability. The concentration of sperm cells decreased from class H to A to L. Fresh semen samples with higher viability and a higher proportion of motile cells also maintained better quality after the freezing and thawing procedure. Sperm viability and motility in both fresh and thawed samples were similar in classes H and A, while significantly lower values were measured in class L. The relative decrease in sperm viability and motility after cryopreservation increased from class H to A to L. The lipid content of spermatozoa (micrograms per 10(9) cells) increased significantly after freezing and thawing in classes H and A but not in class L. This result indicated that active sperm lipid metabolism might be responsible for the increase in lipid content. Phospholipid and triacylglycerol contents increased whereas free cholesterol content decreased after thawing. The fatty acid composition of fresh spermatozoa was similar in all three classes. The proportion of polyunsaturated fatty acids decreased significantly after freezing and thawing, indicating contamination from the diluent or peroxidation. After freezing and thawing, superoxide dismutase activity in spermatozoa was significantly higher in class L than in classes H and A, which did not differ from each other.

scholarly journals Fungi and Aflatoxin Levels in Traditionally Processed Cassava (Manihot esculenta Crantz) Products in Homa Bay County, Kenya

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Boniface Oure Obong’o ◽  
George Ayodo ◽  
Fanuel Kawaka ◽  
Morelly Kathy Adalla
Keyword(s):  
Manihot Esculenta ◽  
Candida Parapsilosis ◽  
Candida Rugosa ◽  
Fungal Species ◽  
Aflatoxin Contamination ◽  
East African ◽  
Manihot Esculenta Crantz ◽  
Safety Standards ◽  
Traditional Processing ◽  
The Tropics

Cassava (Manihot esculenta Crantz) is a major source of carbohydrates, calcium, vitamins (B and C), and essential minerals and is the third most important source of calories in the tropics. However, it is not clear if the traditional processing methods expose the products to microbial contamination. This study assessed the levels of fungi and aflatoxin contamination in traditionally processed cassava products (Akuoga and Abeta). A total of 38 samples were collected from the local markets in 7 subcounties in Homa Bay County, Kenya. The levels of aflatoxin were determined using an indirect competitive ELISA protocol. Yeast and mould contamination was determined using ISO 21527-2 method. Mean aflatoxin levels in chopped, fermented, and sun-dried cassava (Akuoga) were 0.36 μg/kg compared to 0.25 μg/kg in chopped and sun-dried (Abeta) products. Aflatoxin contamination was detected in 55% of the samples and ranged from 0–5.33 μg/kg. These levels are within 10 μg/kg recommended by the CODEX STAN 193-1995. Yeast and mould counts in fermented and chopped sun-dried products were 3.16 log Cfu/g and 2.92 log Cfu/g, respectively. The yeast and mould counts were above standards set by East African Standard 739:2010 in 62% (Akuoga) and 58% (Abeta). The most prevalent fungal species were Saccharomyces cerevisiae (68.4%) and Candida rugosa (68%) followed by Candida parapsilosis (18.4%), Candida tropicalis (15.8%), Candida humilis (15.8%), and Aspergillus spp. (5.3%). Aspergillus spp. was the only mycotoxigenic mould isolated from the samples. The study shows that cassava consumers are exposed to the risk of aflatoxin poisoning. The study, therefore, recommends appropriate surveillance to ensure safety standards.

scholarly journals Mycobiota of Commercially Available Triphala Powder: A Well Known Dietary Supplement of Indian System of Medicine

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Sushil Sharma ◽  
Madhu Gupta ◽  
Rekha Bhadauria
Keyword(s):  
Moisture Content ◽  
Quality Standards ◽  
Fungal Species ◽  
Low Ph ◽  
High Moisture Content ◽  
Fungal Contamination ◽  
High Moisture ◽  
Predominant Species ◽  
Fungal Load ◽  
Percent Moisture

Sixty samples, categorized on the basis of manufacturers, were analyzed during the study. A total of 16 fungal species, belonging to 7 different genera, were isolated from the collected samples. Aspergillus was recorded as the most dominant genus with 9 species, namely, A. niger, A. carbonarius, A. luchuensis, A. fumigatus, A. flavus, A. nidulans, A. terreus, A. ochraceous, and A. wentii. A. niger was the most predominant species with frequency of occurrence of 63.33%. A large variation in fungal load and diversity was observed among the samples of different manufacturing categories. The percent moisture content and pH of samples were directly related to the extent of contamination. Samples with low pH and high moisture content were more contaminated. The higher incidence of A. niger (74.36%) was observed among the triphala powder of all manufacturing categories. Detection of ochratoxin producing fungi in triphala powder may pose a serious risk of ochratoxin production. Thus, there is an urgent need to enforce quality standards and regulation to minimize the fungal contamination to the globally expectable limit.

BOAR, BREED AND ENVIRONMENTAL EFFECTS ON MOTILITY OF FROZEN-THAWED SPERMATOZOA

1986 ◽  
Vol 66 (3) ◽  
pp. 663-668 ◽  
Author(s):  
S. M. JOYAL ◽  
B. W. KENNEDY ◽  
J. N. WILKINS
Keyword(s):  
Key Words ◽  
Environmental Effects ◽  
Current Practice ◽  
Volume Concentration ◽  
Motile Sperm ◽  
Freezing And Thawing ◽  
Frozen Semen ◽  
Boar Semen ◽  
Fresh Semen

A total of 5186 ejaculates from 115 boars of five breeds (48 Yorkshire, 25 Landrace, 20 Duroc, 18 Hampshire and four Lacombe) were collected and frozen between 1975 and 1980. Frozen semen was thawed and evaluated for percentage progressive motile sperm (post-thaw rating) and loss in percentage motile sperm due to freezing and thawing. Boar repeatabilities and effects of breed of boar, year, month, age of boar at collection and percentage of extender added were examined. Boar repeatabilities were 0.32 for post-thaw rating and 0.22 for loss in percentage motile sperm. Breed of boar was not significant. Year was significant, and percentage motile sperm recovered declined from 1976 through 1980. Semen collected between March and May had the highest post-thaw rating. As age of boar increased, post-thaw rating declined and loss in percentage motile sperm increased. As the percentage of extender added to semen increased, so did the loss in percentage motile sperm after freezing. Correlations between post-thaw rating and fresh semen motility score, volume, concentration and percentage motile sperm were 0.24, 0.03, −0.20 and 0.25, respectively. Respective correlations of fresh semen measures with loss in percentage motile sperm after freezing were 0.15, 0.01, 0.17 and 0.23. The current practice of standardizing fresh semen to a given concentration before freezing does not result in a standard frozen-thawed product with respect to percentage motile sperm. Key words: Boar, semen, freezing, thawing, motility

scholarly journals Fungal Load, Coliform and Aflatoxins in Wheat Flour of Lahore Metropolitan City

2020 ◽  
pp. 93-99
Keyword(s):  
Wheat Flour ◽  
Microbiological Quality ◽  
Total Coliform ◽  
Fungal Species ◽  
Coliform Bacteria ◽  
Whole Wheat Flour ◽  
Whole Wheat ◽  
Fungal Load ◽  
Wheat Flours

In Pakistan, wheat flour is mostly utilized for making flat breads locally called chapattis. Other uses of wheat flour are in bakery products manufacturing. It is an important constituent of daily diet of people. Shelf life of wheat flour is one of the most important factors for its quality determination. Wheat flour is often tainted with pathogenic fungal species and their toxic secondary metabolites called Mycotoxins. The present study was designed to make a comparative analysis of Microbiological quality (fungal load and total coliform count) and detection of aflatoxins in raw and branded whole wheat flour samples of Lahore Metropolitan city. Total 100 samples were collected for determination of fungal load, coliform and aflatoxins. The Standardized methods were applied to count the colony forming units of fungal species and total coliform bacteria respectively. The results indicated both branded and raw whole wheat flour groups were of good quality for human consumption. However, the microbiological quality of branded whole flour was better than raw whole wheat flour. There was a significant difference (P<0.05) in fungal load of raw and branded whole wheat flour. Similarly, coliform bacteria were only cultured form the samples of raw flour. In the last stage of study, aflatoxins level was analysed by using a commercially available kit. All the samples of both raw and branded whole wheat flours were negative for aflatoxins detection. The overall quality of raw and branded whole wheat flours in Lahore city is satisfactory for consumers.

scholarly journals A new sperm selection criterion for cryopreservation of boar semen

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Monika Trzcińska ◽  
Magdalena Bryła
Keyword(s):  
Sperm Quality ◽  
Selection Criterion ◽  
Motile Sperm ◽  
Before And After ◽  
Viable Sperm ◽  
Sperm Membrane ◽  
Boar Semen ◽  
Acrosome Integrity ◽  
Fresh Semen

AbstractThis study aimed to define potential markers that could determine the suitability of ejaculate for cryopreservation. Fresh semen from eleven boars (4–7 ejaculates/boar), regardless of their sperm motility, was subjected to a cryopreservation procedure. The sperm quality before and after freezing was assessed based on the sperm membrane permeability and acrosome integrity. The results showed that it was possible to effectively cryopreserve ejaculates below the accepted standards of 70–80% of fresh motile sperm and still obtain a high cryosurvival rate. Moreover, a significant correlation was found between the percentage of viable sperm with apoptotic-like changes, viable sperm with reacted acrosomes, and the cryosurvival rate. The proposed markers for assessing the quality of fresh semen could be used to predict the success of cryopreservation procedures.

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