BOAR, BREED AND ENVIRONMENTAL EFFECTS ON MOTILITY OF FROZEN-THAWED SPERMATOZOA

1986 ◽  
Vol 66 (3) ◽  
pp. 663-668 ◽  
Author(s):  
S. M. JOYAL ◽  
B. W. KENNEDY ◽  
J. N. WILKINS
Keyword(s):  
Key Words ◽  
Environmental Effects ◽  
Current Practice ◽  
Volume Concentration ◽  
Motile Sperm ◽  
Freezing And Thawing ◽  
Frozen Semen ◽  
Boar Semen ◽  
Fresh Semen

A total of 5186 ejaculates from 115 boars of five breeds (48 Yorkshire, 25 Landrace, 20 Duroc, 18 Hampshire and four Lacombe) were collected and frozen between 1975 and 1980. Frozen semen was thawed and evaluated for percentage progressive motile sperm (post-thaw rating) and loss in percentage motile sperm due to freezing and thawing. Boar repeatabilities and effects of breed of boar, year, month, age of boar at collection and percentage of extender added were examined. Boar repeatabilities were 0.32 for post-thaw rating and 0.22 for loss in percentage motile sperm. Breed of boar was not significant. Year was significant, and percentage motile sperm recovered declined from 1976 through 1980. Semen collected between March and May had the highest post-thaw rating. As age of boar increased, post-thaw rating declined and loss in percentage motile sperm increased. As the percentage of extender added to semen increased, so did the loss in percentage motile sperm after freezing. Correlations between post-thaw rating and fresh semen motility score, volume, concentration and percentage motile sperm were 0.24, 0.03, −0.20 and 0.25, respectively. Respective correlations of fresh semen measures with loss in percentage motile sperm after freezing were 0.15, 0.01, 0.17 and 0.23. The current practice of standardizing fresh semen to a given concentration before freezing does not result in a standard frozen-thawed product with respect to percentage motile sperm. Key words: Boar, semen, freezing, thawing, motility

scholarly journals Changes in sperm quality and lipid composition during cryopreservation of boar semen

Reproduction ◽  
2001 ◽  
pp. 395-401 ◽  
Author(s):  
S Cerolini ◽  
A Maldjian ◽  
F Pizzi ◽  
TM Gliozzi
Keyword(s):  
Lipid Content ◽  
Free Cholesterol ◽  
Sperm Quality ◽  
Cholesterol Content ◽  
Sperm Viability ◽  
Freezing And Thawing ◽  
Motile Cells ◽  
Boar Semen ◽  
Thawing Procedure ◽  
Fresh Semen

The effect of cryopreservation on boar sperm viability, motility, lipid content and antioxidant enzymatic activities was studied. Three classes of semen were determined according to a cluster analysis on the basis of the proportion of live and dead cells after freezing and thawing. The classes identified were: high (H, n = 4), average (A, n = 12) and low (L, n = 3) viability. The concentration of sperm cells decreased from class H to A to L. Fresh semen samples with higher viability and a higher proportion of motile cells also maintained better quality after the freezing and thawing procedure. Sperm viability and motility in both fresh and thawed samples were similar in classes H and A, while significantly lower values were measured in class L. The relative decrease in sperm viability and motility after cryopreservation increased from class H to A to L. The lipid content of spermatozoa (micrograms per 10(9) cells) increased significantly after freezing and thawing in classes H and A but not in class L. This result indicated that active sperm lipid metabolism might be responsible for the increase in lipid content. Phospholipid and triacylglycerol contents increased whereas free cholesterol content decreased after thawing. The fatty acid composition of fresh spermatozoa was similar in all three classes. The proportion of polyunsaturated fatty acids decreased significantly after freezing and thawing, indicating contamination from the diluent or peroxidation. After freezing and thawing, superoxide dismutase activity in spermatozoa was significantly higher in class L than in classes H and A, which did not differ from each other.

scholarly journals A new sperm selection criterion for cryopreservation of boar semen

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Monika Trzcińska ◽  
Magdalena Bryła
Keyword(s):  
Sperm Quality ◽  
Selection Criterion ◽  
Motile Sperm ◽  
Before And After ◽  
Viable Sperm ◽  
Sperm Membrane ◽  
Boar Semen ◽  
Acrosome Integrity ◽  
Fresh Semen

AbstractThis study aimed to define potential markers that could determine the suitability of ejaculate for cryopreservation. Fresh semen from eleven boars (4–7 ejaculates/boar), regardless of their sperm motility, was subjected to a cryopreservation procedure. The sperm quality before and after freezing was assessed based on the sperm membrane permeability and acrosome integrity. The results showed that it was possible to effectively cryopreserve ejaculates below the accepted standards of 70–80% of fresh motile sperm and still obtain a high cryosurvival rate. Moreover, a significant correlation was found between the percentage of viable sperm with apoptotic-like changes, viable sperm with reacted acrosomes, and the cryosurvival rate. The proposed markers for assessing the quality of fresh semen could be used to predict the success of cryopreservation procedures.

Comparative Evaluation of Motility and Kinematics of Fresh Versus Frozen-Thawed Spermatozoa of Cattle and Buffalo Bull by CASA

2019 ◽  
Author(s):  
P K Pathak ◽  
A J Dhami ◽  
D V Chaudhari ◽  
K K Hadiya
Keyword(s):  
Sperm Motility ◽  
Motile Sperm ◽  
Mean Values ◽  
Head Displacement ◽  
Straight Line ◽  
Frozen Semen ◽  
Bovine Spermatozoa ◽  
Motion Characteristics ◽  
Fresh Semen

A study was undertaken on semen of three mature bulls each of Gir, Surti and Murrah breed to evaluate the comparative motion characteristics and kinematics of their fresh and frozen-thawed spermatozoa by Biovis CASA. The ejaculates (n= 24/breed) having >75% initial motility were diluted @ 80 million sperm/ml using TFYG extender and were assessed. Amongst motility traits, the total motile, rapid progressive motile and slow progressive motile spermatozoa percentage decreased significantly by 23.08 - 30.09, 43.57 - 55.18, 9.12 - 22.75 %, respectively, plessthan0.01), while non-progressive motile sperm (4.78 - 21.48%) and immotile sperm (164.38 - 178.38 %) percentage increased significantly ( plessthan0.01) in frozen-thawed semen compared to that of fresh semen. The post-thaw quality of semen of all three breeds was in acceptable range. The mean values of sperm velocity/kinematic parameters observed in frozen-thawed semen of Gir, Surti and Murrah bulls, based on total motile sperms, viz., average path velocity, curvilinear velocity, straight line velocity, linearity, straightness, beat-cross frequency, amplitude of lateral head displacement, dancing frequency and dancing mean decreased significantly by 13.70 - 17.79; 9.76 - 12.95; 13.28 - 21.90; 7.28 - 9.68; 4.36 - 7.79; 15.56 - 25.15; 8.78 - 10.50; 6.16 - 18.67 and 12.98 - 15.96 %, respectively, as compared to that of their fresh semen samples. However, wobbling index remained almost same for both fresh and frozen semen. All motility traits differed but none of kinematics/velocity traits differed significantly between breeds/species. The values of all velocity parameters for progressive motile sperms were higher than total motile sperms in all three breeds. The effect of freezing-thawing on velocity and kinematic attributes was much less compared to absolute sperm motility, and both the fresh and frozen-thawed sperms behaved identically with respect to their velocity and kinematics. The rapid progressive motile sperm in both fresh (r=0.41 to 0.92) and frozen-thawed (r=044 to 0.88) semen had significant correlations with most of their velocity traits, and the later were significantly and positively or negatively inter-related among each other in semen of all three breeds. It was therefore concluded that cryopreservation process significantly reduces the motility and kinematics attributes of bovine spermatozoa and, CASA analysis of fresh semen for motility and velocity traits could predict the post-thawed sperm motility and velocity/ kinematics of spermatozoa.

scholarly journals Modifications of bull spermatozoa induced by three extenders: Biociphos, low density lipoprotein and Triladyl, before, during and after freezing and thawing

Reproduction ◽  
2005 ◽  
Vol 129 (4) ◽  
pp. 535-543 ◽  
Author(s):  
Lamia Amirat ◽  
Marc Anton ◽  
Daniel Tainturier ◽  
Gérard Chatagnon ◽  
Isabelle Battut ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Membrane Integrity ◽  
Density Lipoprotein ◽  
Freezing Process ◽  
Low Density ◽  
Freezing And Thawing ◽  
Cell Membrane Integrity ◽  
Cell Structures ◽  
Frozen Semen ◽  
Fresh Semen

The success of artificial insemination with frozen semen implies the reduction of the deleterious effects on the cells induced by this technique. These effects can occur as early as during the first dilution in an extender, as well as at any step, during or after the freezing process.In this work, we have compared the modifications induced by Triladyl, low density lipoproteins (LDL) and Biociphos extenders, after dilution and cooling to 4 °C for 1, 4 and 24 h. Alterations in the cell structures were visualized by electron microscopy (EM). More than 80% of spermatozoa were injured after incubation for 4 h in Triladyl, while 3% and 47% were counted in LDL and Biociphos respectively. This latter extender was deleterious to cell membrane integrity after incubation for 4 h or longer.The ultrastructure of frozen spermatozoa was studied by EM of cryofixed-cryosubstituted samples obtained from regular 0.5 ml French straws frozen using our usual protocol. The main differences between samples concerned the size and appearance of the frozen extender veins, while very few cell defects were found to be added by the freezing process at any depth in the straws.After thawing, semen motility was twofold higher (P< 0.05) in Biociphos (64%) and LDL (61%) than in Triladyl (32%) and the cells were less altered in LDL. We concluded that the LDL extender offers a better protection for storage of frozen spermatozoa, and can probably also be used for the preservation of fresh semen for short periods.

scholarly journals Seminal Attributes, Freezability and their Interrelationships in Zebu Cattle and Buffalo Bulls from Central Gujarat

2018 ◽  
Vol 14 (2) ◽  
Author(s):  
P. K. Pathak ◽  
A. J. Dhami ◽  
D. V. Chaudhari
Keyword(s):  
Semen Quality ◽  
Conventional Technique ◽  
Zebu Cattle ◽  
Motile Sperm ◽  
Morphological Attributes ◽  
Frozen Semen ◽  
The Mean ◽  
Live Sperm ◽  
Sperm Output ◽  
Fresh Semen

A study was carried out on nine healthy mature breeding bulls (3 each of Gir, Surti and Murrah breed) to evaluate their fresh and frozen semen quality and their interrelationships. The ejaculates immediately after collection were evaluated for routine physico- morphological attributes, including HOS test. The ejaculates (n=72) having >75% initial motility were diluted @ 80 million sperm/ml using TFYG extender and the French mini straws filled were frozen in liquid nitrogen vapour using a programmable biofreezer. Thawing of straws was done at 37°C for 30 sec and assessed for freezability by conventional technique. All the cattle and buffalo bulls donated consistently normal thick creamy yellow and thick milky white semen, respectively. In Gir, Suti and Murrah bulls (n=24 ejaculate each) the seminal attributes such as ejaculate volume (6.69±0.17, 3.12±0.10 and 3.96±0.16 ml, p less than 0.01); initial motility (80.21±0.88, 84.58±0.60 and 84.38±0.76 %, p less than 0.01); total sperm output/ejaculate (9013.85±265.32, 3935.49±259.63 and 5366.48±332.99 million, p less than 0.01) and live sperm (84.71±0.83, 86.17±0.78 and 86.79±0.79 %, p less than 0.05) differed significantly. The mean percentages of post-thaw motile sperm (53.29±1.56, 58.33±1.43 and 59.58±1.20, p less than 0.01); live sperm (59.00±1.95, 67.00±1.59 and 68.42±1.66 %, p less than 0.01); and HOS reactive sperm (48.25±0.78, 44.21±1.29 and 51.54±1.29 %, p less than 0.01) in Gir, Surti and Murrah bulls semen also differed significantly. The variation among the bulls was significant for buffalo breeds in most of their fresh seminal attributes, except HOST, and for post-thaw motility, but not among Gir bulls. The important seminal attributes like motility, live sperm and HOS reactive sperm of fresh and frozen-thawed semen were significantly and positively interrelated in all three breeds of bulls (r = 0.40 to 0.81, p less than 0.05 to 0.01), suggesting that motility and HOST of fresh semen were good predictors of freezability of bovine semen.

scholarly journals Macro- and microelements in serum and seminal plasma as biomarkers for bull sperm cryotolerance

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Maja Zakošek Pipan ◽  
Petra Zrimšek ◽  
Breda Jakovac Strajn ◽  
Katarina Pavšič Vrtač ◽  
Tanja Knific ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Semen Quality ◽  
Sperm Quality ◽  
Membrane Integrity ◽  
Quality Characteristics ◽  
Freezing And Thawing ◽  
Additional Tool ◽  
Bull Semen ◽  
Bull Sperm ◽  
Fresh Semen

ABSTRACT Background Wide variation in fertility rates is observed when using frozen bull semen, even when the bulls have met quality standards for semen production. Therefore, a simple and reliable test to assess the freezing potential of bull semen based on the analysis of fresh semen or blood would be of great value. Attention is now turning to assessment of seminal plasma components such as proteins and elements. In the present study, the concentrations of macro- and microelements in fresh bull semen plasma and in serum and their correlation with quality characteristics of fresh semen and with semen quality after freezing and thawing were determined. Ejaculates were collected from 30 mature bulls, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability and DNA fragmentation were determined on the day of collection and after freezing and thawing. The concentrations of macroelements (Na, Mg, K and Ca) and microelements (Cu, Fe, Zn and Se) were determined in the seminal plasma and serum. The semen samples were classified into satisfactory and unsatisfactory groups according to the fresh semen quality. Results Zinc and Se levels measured in serum were associated with almost all fresh and frozen-thawed semen quality characteristics, while Fe levels were associated only with acrosomal defects in fresh semen. Zinc and Fe levels in fresh seminal plasma were associated with various quality characteristics of fresh and frozen-thawed semen, while Se level in fresh seminal plasma was not associated with any of the semen quality characteristics. Conclusions Microelements were shown to be useful as biomarkers involved in the analysis of bull sperm quality and could be used as an additional tool to predict bull semen quality after freezing and thawing. Our results confirm that the analysis of Zn and Se levels in serum and Zn, Cu and Fe levels in fresh seminal plasma can provide information to discriminate between bull semen samples with spermatozoa with high or low cryotolerance.

scholarly journals Effect of Oral Administration of Selenium and Vitamin E on the Quality of Fresh, Refrigerated and Frozen Semen in French Bulldog Breed Dogs

2017 ◽  
Vol 45 (1) ◽  
pp. 7
Author(s):  
Marcelo George Mungai Chacur ◽  
Mariana Grandis Ripari de Souza ◽  
Camila Dutra de Souza ◽  
Camila Pires Cremasco
Keyword(s):  
Vitamin E ◽  
Citric Acid ◽  
Oral Administration ◽  
Liquid Nitrogen ◽  
Sperm Concentration ◽  
Oral Supplementation ◽  
Frozen Semen ◽  
French Bulldog ◽  
Fresh Semen

Background: New methodologies have been developed seeking to maximize pregnancy rate in female dogs created in commercial kennels, and also in order to maintain the quality of canine semen after dilution, refrigeration or freezing. One of the main factors that generate damage to sperm is oxidative stress, to minimize sperm damage, selenium and antioxidants like vitamin E are administered, by oral administration, seeking to improve the quality of semen. The objective was to study the effect of vitamin E and selenium, by oral administration, in the quality of fresh, refrigerated and frozen semen in adult dogs French Bulldog breed.Materials, Methods & Results: Semen samples were collected from 5 adult dogs, French Bulldog breed, being 2 semen drawing before the daily oral supplementation with vitamin E and selenium (ESE®) and semen drawing at 20, 40 and 60 days after the beginning of oral supplement. The ejaculated samples were diluted in TRIS - fructose citric acid (3.28 g TRIS-hydroxy-methyl-amino-methane, 1.78 g of citric acid monohydrate and 1.25 g of D - fructose, dissolved in 100 mL of distilled water and added of 20% egg yolk and 6% of glycerol. The characteristics evaluated in fresh semen were: volume (mL), color, appearance, concentration (x106 / mL), sperm motility (%), sperm strength (1 to 5) and morphology (%). For refrigerated and frozen semen were analyzed: sperm motility (%), sperm strength (1-5) and morphology (%). Diluted semen samples were centrifuged at: 1500 g/10 min and “pellets” formed by sperm of each ejaculated, detached from the tube wall were diluted homogeneously in the diluent TRIS type up to the final volume of 1.5 mL. After that, packaged in 0.5 mL French straws, kept under refrigeration at 5ºC/4 h, placed in nitrogen vapor at -120ºC/15 min, and dipped in liquid nitrogen at -196ºC and then stored on identified rachis and stored in liquid nitrogen container until the time of thawing in  water bath at 37°C/30 s for semen microscopic analysis. Data from fresh, refrigerated and frozen semen were statistically analyzed by analysis of variance and the average compared by 5% of Tukey test. Fresh semen sperm concentration differed (P < 0.05) between the samples, rising after 40 days after the beginning of oral supplementation with selenium and vitamin E. For the spermatic strength, better score (P < 0.05) was observed at collection 4, in 40 days after the beginning of oral supplementation to dogs. For fresh and refrigerated semen, the total defects, defects of head, acrosome and tail did not differ (P > 0.05) between the samples. Total sperm defects and minor head and tail defects did not differ (P > 0.05) between the samples in post-thawing. Regarding the acrosome defects after thawing, there was a significant reduction (P < 0.05) in samples performed 40 and 60 days after the beginning of oral supplementation with selenium and vitamin E.Discussion: Attention should be paid for what purpose the extenders within the refrigeration or freezing biotech will be used. The managed supplement, by oral administration, containing selenium and vitamin E, influenced beneficially raising the sperm concentration in fresh semen and decreasing the acrosome defects in frozen semen. Oral administration of supplementation with selenium and vitamin E is recommended for improving the quality of fresh and frozen semen in dogs.

Evaluation of intrauterine insemination of sheep with frozen semen: effects of time of insemination and semen dose on conception rates

1991 ◽  
Vol 53 (1) ◽  
pp. 89-96 ◽  
Author(s):  
R. C. F. Findlater ◽  
W. Haresign ◽  
R. M. Curnock ◽  
N. F. G. Beck
Keyword(s):  
Intrauterine Insemination ◽  
Field Application ◽  
Conception Rate ◽  
Laparoscopic Technique ◽  
Motile Sperm ◽  
Treatment Groups ◽  
Frozen Semen ◽  
Significant Difference ◽  
Conception Rates ◽  
Serum Gonadotropin

ABSTRACTThe field application of a laparoscopic technique to permit intra-uterine insemination of ewes with frozen-thawed semen was examined in two trials, conducted over successive years, to (i) determine the optimum time of insemination relative to sponge removal/pregnant mares's serum gonadotropin (PMSG) injection and (ii) establish the relationship between semen dose and conception rate. Pooled semen was used in both trials, and each involved > 900 ewes in a number of commercial flocks.Maximum conception rates were achieved when insemination was conducted between 54 h and 60 h after sponge removal/ PMSG injection. However, there was no significant difference in conception rate when motile sperm numbers were reduced from 52·2 × 106to 13·0 × 106 motile sperm per uterine horn.The overall conception rates (pooled over flocks and treatment groups) were 56% and 58% for the two trials, with a wide degree of variation between flocks in both cases (45% to 79% for trial 1 and 45% to 69% for trial 2). However, there was evidence for consistent differences in conception rates between the six flocks involved in both years of the trials.

scholarly journals Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine

2016 ◽  
Vol 37 (4) ◽  
pp. 2167 ◽  
Author(s):  
Mayara Setúbal Oliveira ◽  
Priscila Silva de Almeida-Monteiro ◽  
Larissa Teixeira Nunes ◽  
Francisco Renan Aragão Linhares ◽  
João Paulo Silva Pinheiro ◽  
...  
Keyword(s):  
Dimethyl Sulfoxide ◽  
Dilution Rate ◽  
Freshwater Fish ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Motile Sperm ◽  
Colossoma Macropomum ◽  
Freezing Method ◽  
In Captivity ◽  
Fresh Semen

Tambaqui (Colossoma macropomum) is a native freshwater fish that is of great importance for Brazilian aquaculture. Because of this importance, several techniques have been developed to improve the reproduction of this species in captivity. One of these techniques is the cryopreservation of sperm. In an effort to increase the efficiency of cryopreservation protocols, researchers have tried to determine suitable diluting solutions and freezing methods, which will provide a better post-thaw sperm quality. Thus, this study aimed to evaluate the efficiency of different diluents and freezing methods for the cryopreservation of tambaqui (C. macropomum) sperm. Samples of fresh semen were diluted in different treatments (Glucose 5% + 10% Dimethyl sulfoxide – DMSO, Glucose 5% + 10% Methyl glycol – MG, BTS + 10% DMSO and BTS + 10% MG) at a 1:9 dilution rate and frozen in a programmed freezing machine and a dry shipper. The semen samples were thawed and evaluated for vitality, sperm morphology and kinetics. Cryopreserved semen with DMSO and using the programmed freezing machine provided a greater percentage of motile sperm (15.44 ± 1.04%) after thawing compared to the dry shipper (3.99 ± 0.55%), regardless of the diluent. Additionally, DMSO showed better sperm velocities than MG regardless of the freezing method and the extender employed. A higher percentage of living spermatozoa was observed when glucose (37.28 ± 1.32%) (regardless of the freezing method and cryoprotectant) and DMSO (37.98 ± 1.25%) was used in the programmed freezing machine. For morphology, a greater amount of normal spermatozoa (46.10 ± 1.82%) was observed when the semen was cryopreserved using a freezing machine programmed with DMSO as the cryoprotectant and Glucose or BTS (38.16 ± 1.9% and 39.26 ± 1.87%, respectively) as extenders. Therefore, we suggest the use of the DMSO (10%) cryoprotectant in association with the Glucose (5%) extended in the programmed freezing machine for cryopreservation of C. macropomum semen.

Sign in / Sign up

Export Citation Format

Share Document