scholarly journals Antibacterial Activity of Biosynthesized Selenium Nanoparticles Using Extracts of Calendula officinalis against Potentially Clinical Bacterial Strains

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5929
Author(s):  
José A Hernández-Díaz ◽  
Jorge JO Garza-García ◽  
Janet M León-Morales ◽  
Adalberto Zamudio-Ojeda ◽  
Jenny Arratia-Quijada ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Bacterial Resistance ◽  
Alcaligenes Faecalis ◽  
Selenium Nanoparticles ◽  
Bacterial Strains ◽  
Calendula Officinalis ◽  
Methanolic Extracts ◽  
Antimicrobial And Antioxidant Activity

The use of selenium nanoparticles (SeNPs) in the biomedical area has been increasing as an alternative to the growing bacterial resistance to antibiotics. In this research, SeNPs were synthesized by green synthesis using ascorbic acid (AsAc) as a reducing agent and methanolic extract of Calendula officinalis L. flowers as a stabilizer. Characterization of SeNPs was performed by UV-vis spectrophotometry, infrared spectrophotometry (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM) techniques. SeNPs of 40–60 nm and spherical morphologies were obtained. The antibacterial activity of marigold extracts and fractions was evaluated by disk diffusion methodology. The evaluation of SeNPs at different incubation times was performed through the colony-forming unit (CFU) count, in both cases against Serratia marcescens, Enterobacter cloacae, and Alcaligenes faecalis bacteria. Partial antibacterial activity was observed with methanolic extracts of marigold leaves and flowers and total inhibition with SeNPs from 2 h for S. marcescens, 1 h for E. cloacae, and 30 min for A. faecalis. In addition, SeNPs were found to exhibit antioxidant activity. The results indicate that SeNPs present a potentiated effect of both antimicrobial and antioxidant activity compared to the individual use of marigold extracts or sodium selenite (Na2SeO3). Their application emerges as an alternative for the control of clinical pathogens.

scholarly journals Alkaloid-Rich Crude Extracts, Fractions and Piperamide Alkaloids of Piper guineense Possess Promising Antibacterial Effects

Antibiotics ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 98 ◽  
Author(s):  
Eunice Mgbeahuruike ◽  
Pia Fyhrquist ◽  
Heikki Vuorela ◽  
Riitta Julkunen-Tiitto ◽  
Yvonne Holm
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Hot Water ◽  
Bacterial Strains ◽  
Inhibitory Effects ◽  
Piper Guineense ◽  
E Coli ◽  
Growth Inhibitory ◽  
Derivatives Of

Piper guineense is a food and medicinal plant commonly used to treat infectious diseases in West-African traditional medicine. In a bid to identify new antibacterial compounds due to bacterial resistance to antibiotics, twelve extracts of P. guineense fruits and leaves, obtained by sequential extraction, as well as the piperine and piperlongumine commercial compounds were evaluated for antibacterial activity against human pathogenic bacteria. HPLC-DAD and UHPLC/Q-TOF MS analysis were conducted to characterize and identify the compounds present in the extracts with promising antibacterial activity. The extracts, with the exception of the hot water decoctions and macerations, contained piperamide alkaloids as their main constituents. Piperine, dihydropiperine, piperylin, dihydropiperylin or piperlonguminine, dihydropiperlonguminine, wisanine, dihydrowisanine and derivatives of piperine and piperidine were identified in a hexane extract of the leaf. In addition, some new piperamide alkaloids were identified, such as a piperine and a piperidine alkaloid derivative and two unknown piperamide alkaloids. To the best of our knowledge, there are no piperamides reported in the literature with similar UVλ absorption maxima and masses. A piperamide alkaloid-rich hexane leaf extract recorded the lowest MIC of 19 µg/mL against Sarcina sp. and gave promising growth inhibitory effects against S. aureus and E. aerogenes as well, inhibiting the growth of both bacteria with a MIC of 78 µg/mL. Moreover, this is the first report of the antibacterial activity of P. guineense extracts against Sarcina sp. and E. aerogenes. Marked growth inhibition was also obtained for chloroform extracts of the leaves and fruits against P. aeruginosa with a MIC value of 78 µg/mL. Piperine and piperlongumine were active against E. aerogenes, S. aureus, E. coli, S. enterica, P. mirabilis and B. cereus with MIC values ranging from 39–1250 µg/mL. Notably, the water extracts, which were almost devoid of piperamide alkaloids, were not active against the bacterial strains. Our results demonstrate that P. guineense contains antibacterial alkaloids that could be relevant for the discovery of new natural antibiotics.

scholarly journals Biosynthesized gold nanoparticles-doped hydroxyapatite as antibacterial and antioxidant nanocomposite

2021 ◽  
Author(s):  
Is Fatimah ◽  
Putwi Widya Citradewi ◽  
Amri Yahya ◽  
Bambang Nugroho ◽  
Habibi Hidayat ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Antioxidant Activity ◽  
Particle Size ◽  
Gold Nanoparticles ◽  
Antibacterial Activity ◽  
X Ray Diffraction ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Abstract The composite of green synthesized gold nanoparticles (Au NPs)-doped hydroxyapatite (HA) has been prepared. The gold nanoparticles were produced via bioreduction of HAuCl4 with Clitoria ternatea flower extract, and utilized in the synthesis of hydroxyapatite using Ca(OH)2 and ammonium diphosphate as precursor. The aim of this research is to study the structural analysis of the composite and antibacterial activity test toward Eschericia coli, Staphylococcus aureus, Klebsiela pneumoniae, and Streptococcus pyogenes. In addition, the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method. The monitoring of gold nanoparticles formation was conducted by UV–vis spectroscopy and particle size analyses, meanwhile the synthesized composite was studied using X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The results showed that homogeneously dispersed gold nanoparticles in HA structure was obtained with the particle size ranging at 5-80 nm. The nanocomposite demonstrated antibacterial activity against tested bacteria. The nanocomposite expressed an antioxidant activity as shown by the DPPH scavenging activity of 66 and 58% at the concentration of 100 μg/mL and 50 μg/mL, respectively.

scholarly journals PREPARATION OF JUNIPERUS EXTRACT AND DETECTION OF ITS ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY

2019 ◽  
Vol 50 (4) ◽  
Author(s):  
Maeh &et al.
Keyword(s):  
Prostate Cancer ◽  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Inhibition Activity ◽  
Cytotoxicity Activity ◽  
Juniperus Phoenicea ◽  
Antimicrobial And Antioxidant Activity

 This study was aimed to produce silver nanoparticles by fruits extract of Juniperus phoenicea and comparison between the ethanolic extract and nano extract through the antioxidant, antibacterial, anti-parasite and cytotoxicity against prostate cancer. The synthesis of silver nanoparticles was shown by many characterizing techniques: UV, FTIR, XRD and SEM. Nano extract exhibited a higher antibacterial, antioxidant, antiparasite compared to ethanolic extract and higher cytotoxicity activity. The nano extract exhibited higher antibacterial activity compared with antibiotics. Finally, we study the toxicity of J. phoenicea by Inhibition of RBC hemolysis by H2O2, the results exhibited the highest inhibition activity of J. phoenicea against H2O2. The nanoextract of J. phoenicea can be used effectively in the production of potential antioxidant, antiparasite antimicrobial and anticancer.

scholarly journals The in vitro antimicrobial and antioxidant activity of leaves of medicinal plants with phytobiotic potential in animal production

2021 ◽  
Author(s):  
Roisbel Aroche ◽  
Xianren Jiang ◽  
Yordan Martínez ◽  
Román Rodríguez ◽  
Xilong Li ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Moringa Oleifera ◽  
Psidium Guajava ◽  
Anacardium Occidentale ◽  
Morinda Citrifolia ◽  
Bacterial Strains ◽  
Antimicrobial And Antioxidant Activity

Abstract Little is known about which secondary metabolites are responsible for inhibiting pathogenic bacteria and reducing the pro-oxidant effect on the leaves of four medicinal plants used as phytobiotic in animal production. The aim of this study was to evaluate the antimicrobial and antioxidant activity of four medicinal plants (Anacardium occidentale, Psidium guajava, Morinda citrifolia and Moringa oleifera.) in vitro. A total of six bacterial strains were inoculated, then minimum bactericidal concentration (MBC) was evaluated in fine powder and minimum inhibitory concentration (MIC) and MBC were determined on the aqueous extract. Also, the in vitro antioxidant activity was evaluated through 1,1-diphenyl-2-picryl-hydrazyl, as well as the main secondary metabolites were identified and quantified by chromatographic analysis. The results showed that Anacardium occidentale and Psidium guajava leaves had higher antimicrobial activity against all bacterial strains. In addition, Morinda citrifolia inhibited S. aureus in the aqueous extract, although without in vitro bactericidal effect, while Moringa oleifera leaf did not show antimicrobial effect. All plants showed antioxidant capacity, standing out Anacardium occidentale and Psidium guajava. Mainly the leaves of Anacardium occidentale showed high concentrations of quercetin 3-O-glucoside-7-O-rhamnoside, kaempeferol-7-O-glucoside, quercetin, caffeic acid, and cinnamic acid. Apparently, the antimicrobial and antioxidant activity are due to the main polyphenolic compounds identified in medicinal plants (mainly Anacardium occidentale and Psidium guajava); however, further studies are necessary to elucidate the exact mechanism.

scholarly journals ASSESSMENT OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF AMOMUM SUBULATUM ROXB. OF NEPAL

2017 ◽  
Vol 10 (4) ◽  
pp. 95
Author(s):  
Bimala Subba ◽  
Tank Raj Seling ◽  
Ram Chandra Kandel ◽  
Ganga Prasad Phuyal
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antibacterial Drug ◽  
Traditional Use ◽  
Low Altitude ◽  
Amomum Subulatum ◽  
Antimicrobial And Antioxidant Activity ◽  
Antibacterial And Antioxidant Activity

Objective: The present study was designed to study and compare the antibacterial and antioxidant activity of methanol extracts of Amomum subulatum Roxb. seeds collected from two different regions of Nepal.Methods: Antioxidant activity was determined by 1, 1-diphenyl-2-picrylhydrazyl assay and Antibacterial activity was measured by agar well-diffusion method.Results: Results showed that both sample extracts had interesting antibacterial activity against all test microorganisms and had remarkable radical scavenging potential to be used as an antioxidant. Sample B (i.e., collected from high altitude Taplejung district) exerted comparative quit higher antimicrobial and antioxidant activity than the Sample A (i.e., is collected from low altitude Panchthar district). However, the standard antibacterial drug Tetracycline exhibits superior activity than the extracts.Conclusions: The results suggest that both samples (i.e., seed extract of A. subulatum Roxb.) possess almost similar moderate antibacterial and antioxidant activity in comparison to standards which justifies the traditional use of this plant.Keywords: Extract, Cardamom, Activity, Medicinal plants, Seeds, Bacillus pumilus.

scholarly journals Polymer Capped Silver Nanoparticles from Ziziphus nummularia Leaves Extract: Potent Antibacterial and Antioxidant Activity

2021 ◽  
Vol 18 (4) ◽  
pp. 691-701
Author(s):  
Faruk Arodiya ◽  
Chirag Makvana ◽  
Kokila Parmar
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Fourier Transform ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Ziziphus Nummularia ◽  
Antimicrobial And Antioxidant Activity

Generally, synthesis and encapsulation process improve therapeutic value of nano encapsulated drugs. The silver nanoparticles (AgNPs) biosynthesized from Ziziphus nummularia leaves and encapsulated with polyvinyl pyrrolidone (PVP) polymer as antibacterial agents, due to its high bioavailability, better encapsulation and less toxic properties. The nanoparticles (AgNPs) biosynthesized from Ziziphus nummularia leaves and capped with polyvinyl pyrrolidone (PVP) polymer, The acquired AgNPs and polymeric functionalized AgNPs were fully characterised by the UV- Visible spectroscopy , Transmission electron microscopy (TEM), X-Ray diffraction pattern (XRD) and Fourier transform infrared spectroscopy (FTIR).The crystalline Ag NPs and Polymer Functionalized AgNPs have a face-centered cubic structure with an average size of 9.20 nm, according to X-ray Diffraction spectroscopy. Fourier Transform Infrared spectroscopy revealed that biomolecules such as proteins are incapable of reducing metal ions and the formation of an encapsulating layer in terms of metal ions. High-Resolution transmission electron microscopy revealed that Polymer functionalized AgNPs ranged in size of 10 nm. AgNPs and Polymer functionalized AgNPs showed effective antimicrobial and antioxidant activity. The biosynthesized monodisperse silver nanoparticles and encapsulated silver nanoparticles demonstrated better antimicrobial and antioxidant activity which can be used in various biomedical applications.

scholarly journals Short Period Storage Impact on Bioactive Constituents from Bilberries and Blueberries

2017 ◽  
Vol 63 (2) ◽  
pp. 87-90 ◽  
Author(s):  
Ruxandra Emilia Ştefănescu ◽  
Sigrid Eșianu ◽  
Eszter Laczkó-Zöld ◽  
Anca Mare ◽  
Bianca Tudor ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Storage Conditions ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Phenols ◽  
Bioactive Constituents ◽  
Microdilution Method ◽  
Methanolic Extracts ◽  
Short Period

Abstract Objectives: The aim of this study was to assess storage effects on anthocyanin and total polyphenol content in different bilberry and blueberry extracts and to evaluate the antioxidant and antibacterial activity of these extracts. Materials and methods: Total phenolic content, total monomeric anthocyanin content and antioxidant activity were determined in the first month and after three months storage of berries at either -20 °C or -50 °C. Two different solvents were used (methanol and 50% ethanol). Antibacterial activity was determined for the 3 months stored fruits using a microdilution method and was expressed as the minimum inhibitory concentration. Results: There were significant differences between the concentration in the first month and after three months storage in both types of fruit extracts. Regarding the extracting solvent, we noticed that total phenols were better extracted with 50% ethanol, while the total monomeric anthocyanin content was higher in the methanolic extracts. No significant or slightly significant differences were observed between the fruits stored at -20 °C or -50 °C. Ethanolic extracts showed the highest scavenging activity. Good antibacterial activity was observed on gram-positive bacteria. Conclusions: Storage conditions are an important factor that can influence chemical composition of fruits. Although freezing is a good option for preservation, our study showed a high decrease in the concentration of total phenols and anthocyanins after only three months. The fruits have shown a high antioxidant activity and a good antibacterial effect. Further studies are needed for better understanding the changes that can appear during the storage.

scholarly journals Endophytic Fungi from Alstonia boonei De Wild and Greenwayodendron suaveolens (Engl. and Diels) Verdc. subsp. Suaveolens Possess Inhibitory Activity against Pneumonia Causing Bacteria

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Paola Cynthia Emoh Demeni ◽  
Patrick Hervé Diboue Betote ◽  
Christelle Wayoue Kom ◽  
Eric Ngalani Tchamgoue ◽  
Esther Del Florence Ndedi Moni ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Endophytic Fungi ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Methanolic Extracts ◽  
Bactericidal Effects ◽  
Inhibition Zones

This study evaluated the antibacterial efficacy of methanolic extracts of isolated endophytic fungi from stem barks and leaves of Alstonia boonei De Wild and Greenwayodendron suaveolens (Engl. and Diels) Verdc. subsp. Suaveolens against Klebsiella pneumoniae ATCC 43816, Haemophilus influenzae ATCC 49247, Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 35218, responsible for causing pneumonia. The endophytic fungi were isolated and characterized in the Potato Dextrose Agar (PDA), Sabouraud Dextrose Agar (SDA), and Czapek Dox Agar (CDA) media. The fungi and their methanolic extracts were tested for in vitro antibacterial potential by antagonistic assay for endophytic fungi against bacterial pathogens and microdilution method. The phytochemical screening of extracts was carried out according to the colorimetric and precipitation methods to reveal the presence of secondary metabolites. The results showed that 24 macroscopically and microscopically distinct endophytic fungi were isolated, identified, and stored. These endophytic fungi possessed antibacterial activity against the selected bacterial strains with inhibition zones ranging from 7.00 to 25.00 mm. The endophytic fungi GS15 and AB24 have presented the inhibitions zones of 20.33 mm and 25.00 mm, respectively, and these were better than the ones obtained for Levofloxacin®. The endophytes with inhibition zones greater than 10 mm were used for extraction of their secondary metabolites. The endophytic fungi extracts showed antibacterial activity with the minimum inhibitory concentrations (MICs) ranging from 6.25 × 10−4 to 2 × 10−2 g/L and the minimum bactericidal concentrations (MBCs) ranging from 2.5 × 10−3 to 2 × 10−2 g/L. The endophytic fungi GS15 extract was the most effective extract; it showed bactericidal effects on the tested bacterial strains. The phytochemical screening of the extracts revealed the presence of secondary metabolites classes, responsible for causing the obtained antibacterial activity. Thus, the endophytic fungi methanolic extracts from A. boonei and G. suaveolens have the potential to inhibit the growth of bacteria responsible for nosocomial pneumonia.

scholarly journals Simultaneous Sonochemical Coloration and Antibacterial Functionalization of Leather with Selenium Nanoparticles (SeNPs)

Polymers ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 74
Author(s):  
Tarek Abou Elmaaty ◽  
Khaled Sayed-Ahmed ◽  
Radwan Mohamed Ali ◽  
Kholoud El-Khodary ◽  
Shereen A. Abdeldayem
Keyword(s):  
Electron Microscopy ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Salmonella Typhi ◽  
Point Of View ◽  
Selenium Nanoparticles ◽  
Footwear Industry ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Low Cytotoxicity

The development of antibacterial coatings for footwear components is of great interest both from an industry and consumer point of view. In this work, the leather material was developed taking advantage of the intrinsic antibacterial activity and coloring ability of selenium nanoparticles (SeNPs). The SeNPs were synthesized and implemented into the leather surface by using ultrasonic techniques to obtain simultaneous coloring and functionalization. The formation of SeNPs in the solutions was evaluated using UV/Vis spectroscopy and the morphology of the NPs was determined by transmission electron microscopy (TEM). The treated leather material (leather/SeNPs) was characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). The effects of SeNPs on the coloration and antibacterial properties of the leather material were evaluated. The results revealed that the NPs were mostly spherical in shape, regularly distributed, and closely anchored to the leather surface. The particle size distribution of SeNPs at concentrations of 25 mM and 50 mM was in the range of 36–77 nm and 41–149 nm, respectively. It was observed that leather/SeNPs exhibited a higher depth of shade compared to untreated ones, as well as excellent fastness properties. The results showed that leather/SeNPs can significantly enhance the antibacterial activity against model of bacteria, including Gram-positive bacteria (Bacillus cereus) and Gram-negative bacteria (Pseudomonas aeruginosa, Salmonella typhi and Escherichia coli). Moreover, the resulting leather exhibited low cytotoxicity against HFB4 cell lines. This achievement should be quite appealing to the footwear industry as a way to prevent the spread of bacterial infection promoted by humidity, poor breathability and temperature which promote the expansion of the microflora of the skin.

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