scholarly journals New Insight into the Chemical Composition, Antimicrobial and Synergistic Effects of the Moroccan Endemic Thymus atlanticus (Ball) Roussine Essential Oil in Combination with Conventional Antibiotics

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5850
Author(s):  
Ahmed Nafis ◽  
Marcello Iriti ◽  
Lahcen Ouchari ◽  
Fatima El Otmani ◽  
Najat Marraiki ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Synergistic Effect ◽  
Synergistic Effects ◽  
Micrococcus Luteus ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Conventional Antibiotics ◽  
And Control

This study reported the volatile profile, the antimicrobial activity and the synergistic potential of essential oil (EO) from the Moroccan endemic Thymus atlanticus (Ball) Roussine, in combination with the antibiotics ciprofloxacin and fluconazole for the first time, to the best of our knowledge. The EO chemical composition was determined by gas chromatography coupled to mass spectrometry (GC-MS) analysis and the antimicrobial activity assessed by the disc diffusion method against three Gram positive (Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus) and three Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli and one clinical isolate, Klebsiella pneumonia). The antifungal activity was evaluated in four pathogenic yeasts (Candida albicans, C. glabrata, C. krusei and C. parapsilosis). The minimum inhibition concentration (MIC) and the synergistic effect with ciprofloxacin and fluconazole were determined by the two-fold dilution technique and checkerboard test, respectively. Twenty-one constituents were identified by GC-MS in the EO, including carvacrol (21.62%) and borneol (21.13%) as the major components. The EO exhibited a significant antimicrobial activity with inhibition zones ranging from 0.7 mm to 22 mm for P. aeruginosa and B. subtilis, respectively, and MIC values varying from 0.56 mg/mL to 4.47 mg/mL. The fractional inhibitory concentration index (FICI) values ranged from 0.25 to 0.50 for bacteria and from 0.25 to 0.28 for yeasts. The maximum synergistic effect was observed for K. pneumonia with a 256-fold gain of antibiotic MIC. Our results have suggested that EO from T. atlanticus may be used alone or in association with antibiotics as a new potential alternative to prevent and control the emergence of resistant microbial strains both in the medical field and in the food industry.

scholarly journals Chemical Composition and Antimicrobial Activity of Essential Oil of Heracleum rigens

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Nataraj Jagannath ◽  
Hanumanthaiah Ramakrishnaiah ◽  
Venkatarangaiah Krishna ◽  
Prameela Javarayi Gowda
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Bornyl Acetate ◽  
Klebsiella Pneumonia ◽  
Oil Composition ◽  
Bacterial Strains ◽  
Octyl Acetate ◽  
Total Oil

The essential oil was extracted from the seeds of Heracleum rigens by hydrodistillation and a total of twenty compounds accounting for 98.5% of the total oil composition were identified. Physicochemical properties and chemical composition of the oil was determined by a combination GC/FID and GC/MS analysis. The major compounds identified were bornyl acetate (51.2%), α-pinene (22.6%), limonene (9.62%), octyl acetate (3.94%), p-cymene (2.85%) and γ-terpinene (1.93%). The antimicrobial activity of the oil was screened by the disc diffusion method against nine pathogenic bacterial strains. Maximum antimicrobial activity was noted against Klebsiella pneumonia and Bacillus subtillis. This investigation corroborates the traditional claim of H. rigens as an effective antimicrobial agent.

scholarly journals Chemical Composition and Antibacterial Activity of Berries Essential Oil of Algerian Juniperus thurifera (Var. aurasiaca)

2018 ◽  
Vol 24 (3) ◽  
pp. 240-245
Author(s):  
Lamia Boudjedjou ◽  
Messaoud Ramdani ◽  
Azzeddine Zeraib ◽  
Tarek Benmeddour ◽  
Azzedine Fercha
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Synergistic Effects ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Juniperus Thurifera ◽  
E Coli ◽  
Conventional Antibiotics ◽  
Promising Source

Background: Over the past decade, most antibiotic research programs have focused on finding new compounds with antimicrobial activity. This study aims to investigate the chemical composition and antibacterial activity of the essential oil (EO) extracted from ripe berries of Algerian Juniperus thurifera var. aurasiaca. Methods: The chemical composition of J. thurifera EO extracted by hydrodistillation was analyzed by using the GC-MS technique. Antibacterial activity of EO alone and in combination with three conventional antibiotics was assessed by using disc diffusion method against four bacterial strains. Results: Thirty-five components were identified, representing ~87 % of the oil. The main components were m-mentha-6,8-diene (15.43 %), β-pinene (10.59 %), elemol (8.31 %) and terpinene-4-ol (7.44 %). The essential oil showed strong antibacterial activity against S. aureus and E. coli, but no activity against P. aeruginosa and B. subtilis. Synergistic effects were observed because of the combined application of EO with gentamicin against all strains tested, and with amoxicillin against B. subtilis. Furthermore, the combination of EO/cefazolin demonstrated an additive effect against B. subtilis. In contrast, the combination of EO with amoxicillin and céfazoline revealed antagonistic effects against S. aureus, E. coli, and P. aeruginosa. Conclusion: This is the first report on the chemical composition and antibacterial activity of Algerian juniper berries’ essential oil. The results indicate that the studied EO may be a promising source of antibacterial compounds that could be useful for pharmaceutical applications especially in combination with conventional antibiotics.

Chemical composition, antimicrobial activity and synergistic effects with conventional antibiotics under clinical isolates by essential oil of Hymenaea rubriflora Ducke (FABACEAE)

2020 ◽  
pp. 1-5
Author(s):  
Graziela Claudia da Silva ◽  
Bruno Oliveira de Veras ◽  
Caio Rodrigo Dias de Assis ◽  
Daniela Maria do Amaral Ferraz Navarro ◽  
Dyana Leal Veras Diniz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Synergistic Effects ◽  
Clinical Isolates ◽  
Conventional Antibiotics

scholarly journals Comparison of the Chemical Composition and Antimicrobial Activity of Thymus serpyllum Essential Oils

2015 ◽  
Vol 43 (2) ◽  
pp. 432-438 ◽  
Author(s):  
Aneta WESOŁOWSKA ◽  
Monika GRZESZCZUK ◽  
Dorota JADCZAK ◽  
Paweł NAWROTEK ◽  
Magdalena STRUK
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Micrococcus Luteus ◽  
Gas Chromatography Mass Spectrometry ◽  
E Coli ◽  
Thymus Serpyllum ◽  
Main Components ◽  
Total Oil

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Thymus serpyllum and Thymus serpyllum‘Aureus’ has been investigated by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds (99.67% of the total oil) wereidentified in the essential oil of T. serpyllum. The main components found in the oil were carvacrol (37.49%), -terpinene (10.79%), -caryophyllene (6.51%), p-cymene (6.06%), (E)--ocimene (4.63%) and -bisabolene (4.51%). Similarly, carvacrol (44.93%), -terpinene(10.08%), p-cymene (7.39%) and -caryophyllene (6.77%) dominated in the oil of T. serpyllum ‘Aureus’. A total of forty three compounds wereidentified in this oil, representing 99.49% of the total oil content. On the basis of the obtained data it was proved that the content of 1-octen-3-ol,eucalyptol, (Z)--ocimene, (E)--ocimene, -terpinene, carvacrol methyl ether, germacrene D and -bisabolene was significantly higher for T.serpyllum while T. serpyllum ‘Aureus’ was characterized by a significantly higher content of 3-octanone, 3-octanol, p-cymene, borneol andcarvacrol. The isolated essential oils were evaluated for their antimicrobial activity against nine reference strains (Escherichia coli, Staphylococcusaureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Bacillus cereus, Micrococcus luteus, Proteus vulgaris and Candidaalbicans) by the microdilution technique. Based on this test, the minimum inhibitory concentrations (MIC) of essential oil were calculated. Thevolatile oil obtained from T. serpyllum showed the highest antimicrobial activity relative to the strain of E. coli (MIC=0.025 μL/mL) and to theyeast C. albicans (MIC=0.05 μL/mL). Similarly, a significant antimicrobial activity exhibited T. serpyllum ‘Aureus’ essential oil, although the MICvalues obtained in that case for E. coli and C. albicans strains were twice as high and were respectively 0.05 μL/mL and 0.1 μL/mL.

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oil of Anvillea radiata Coss. & Dur

2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Aerial Parts

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.

scholarly journals In vitro Antimicrobial Properties and Chemical Composition of Santolina chamaecyparissus Essential Oil from Algeria

2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Samah Djeddi ◽  
Khadidja Djebile ◽  
Ghania Hadjbourega ◽  
Zoubida Achour ◽  
Catherine Argyropoulou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Klebsiella Pneumonia ◽  
Aerial Parts

The chemical composition of the essential oil obtained from the aerial parts of Santolina chamaecyparissus L., growing in Algeria, was investigated by GC-MS analyses. A total of 36 compounds were identified, accounting for 91.7% of the essential oil obtained. Camphor (31.1%) and cubenol (17.0%) were the predominant compounds. The potential of the antimicrobial activity was also investigated and the tested sample proved to be very active against Klebsiella pneumonia and Candida albicans (34.1 ± 0.02 mm and 35.0 ± 0.01 mm, respectively). Transverse sections of the leaf and stem of the plant suggest that the essential oil is localized in endogenous and exogenous sites.

scholarly journals Chemical composition and antimicrobial activity of Myrtus communis essential oils from Algeria

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
YACINE MOHAMADI ◽  
TAKIA LOGRADA ◽  
Messaoud Ramdani ◽  
GILLES FIGUEREDO ◽  
PIERRE CHALARD
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Geranyl Acetate ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Myrtus Communis ◽  
Disk Diffusion Method

Abstract. Mohamadi Y, Lograda T, Ramdani M, Figueredo G, Chalard P. 2021. Chemical composition and antimicrobial activity of Myrtus communis essential oils from Algeria. Biodiversitas 22: 933-946.  The objectives of this study were to determine the chemical composition and the antibacterial activities of Myrtus communis essential oils. The aerial parts of the myrtle were collected from nineteen localities of the Algerian coast, and subjected to hydrodistillation. The chemical composition of essential oils was analyzed using gas chromatography coupled with mass spectrometry (GC/MS). The antibacterial activity was evaluated by the disk diffusion method against ten bacterial species. Essential oils of M. communis was pale yellow oils, very fragrant, with an average yield of 0.68 ± 0.6%. Forty-six compounds were identified, representing an average of 99.17 ± 0.6% of total oils. The α-pinene was the most abundant component in the essential oils of all populations studied, with a mean of 50.1 ± 10.3%, followed by 1,8-cineole (22.27 ± 4.32%), limonene (5.16 ± 2.67%), linalool (3.79 ± 2.97%), α-terpineol (2.75 ± 1.12%), geranyl acetate (1.95 ± 0.7%) and methyl eugenol (1.40 ± 0.98%). Myrtenyl acetate was only observed in four populations with significant levels (from 10.66 to 20.94%). Six chemotypes have been identified in these oils. The α-pinene – 1,8-cineole - limonene chemotype was found in the majority of populations. The chemotype of α-pinene - 1,8-cineole - α-terphenyl acetate characterizes the Oued El-Aneb population. The α-pinene - neryl formate - limonene chemotype is observed in the Honaïn population. The remaining populations are characterized by myrtenyl acetate. The essential oils of M. communis were effective against Staphylococcus aureus ATCC 25923, Salmonella enterica ATCC35659, Proteus mirabilis, and Escherichia coli ATCC 25922; however, no significant effect was observed on Klebsiella pneumonia ATCC 700603 and Serratia liquefaciens.

scholarly journals Chemical composition and antimicrobial activity of essential oil from Pyrethrum pulchrum Ledeb.

2018 ◽  
Vol 19 (45) ◽  
pp. 38-43 ◽  
Author(s):  
Uugangerel Erdenetsogt ◽  
Choijamts Gotov ◽  
Kerstin Voigt ◽  
Stefan Bartram ◽  
Wilhelm Boland ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Oil Composition ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Total Oil ◽  
Sporidiobolus Salmonicolor

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Pyrethrum pulchrum Ledeb. were investigated. Dried plant material was hydro-distillated yielding 0.1% of essential oil. The oil was analyzed by GC-MS techniques. Fifty-five compounds were identified representing 99.7% of the total oil composition. Camphor was the predominant compound (33.9%) followed by linalool (21.1%) and α-pinene (9.0%). The antimicrobial activity of the oil was determined using the disk diffusion method against Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), Mycobacterium vaccae and fungi (Candida albicans, Sporidiobolus salmonicolor and Penicillum notatum). The essential oil of P. pulchrum displays an intermediate activity against selected bacteria.

scholarly journals Chemical composition and antimicrobial activity of berry essential oil of Juniperus oxycedrus L. (Cupressaceae) grown wild in Republic of Macedonia

2013 ◽  
Vol 59 ◽  
pp. 41-48 ◽  
Author(s):  
Floresha Sela ◽  
Marija Karapandzova ◽  
Gjose Stefkov ◽  
Ivana Cvetkovikj ◽  
Elena Trajkovska-Dokikj ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Salmonella Enteritidis ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Juniperus Oxycedrus ◽  
Republic Of Macedonia

Chemical composition and antimicrobial activity of essential oil isolated from berries from 2 different samples of Juniperus oxycedrus L. (Cupressaceae), growing wild in Republic of Macedonia was investigated. Performing GC/FID/MS analysis, one hundred components were identified, representing 96.0-98.95% of the oil. The major components were α-pinene (22.54- 27.12%), myrcene (11.26- 15.13%) and limonene (2.78-18.06%). Antimicrobial screening of the J. oxycedrus essential oils was made by disc diffusion and broth dilution method against 16 bacterial isolates of Gram positive and Gram negative bacteria and one strain of Candida albicans. The most sensitive bacteria was Haemophilus influenzae (MIC = 125 ml/ml). The essential oils showed moderate antimicrobial activity against Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus pyogenes, Corynebacterium spp., Escherichia coli and Campilobacter jejuni (MIC > 500 ml/ml) and no activity against Candida albicans, Staphylococcus epidermidis, Acinetobacter spp., Salmonella enteritidis, Shigella flexnery, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterococcus and Proteus mirabilis.

scholarly journals THE CHEMICAL COMPOSITION AND THE ANTIMICROBIAL PROPERTIES OF THE ESSENTIAL OIL EXTRACTED FROM THE LEAVES OF TEUCRIUM CAPITATUM L.

2017 ◽  
Vol 10 (2) ◽  
pp. 112 ◽  
Author(s):  
Jalila El Amri ◽  
Khalid El Badaoui ◽  
Zoubida Haloui
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Chromatographic Analysis ◽  
Diffusion Method ◽  
Ethnobotanical Study ◽  
Microsporum Canis ◽  
Microsporum Gypseum

Objective: In the framework of the search for new biological molecules, an ethnobotanical study has been carried out in the region of El Hajeb in Morocco in the interest of will select the data ethnopharmacological on medicinal and aromatic plants and on the découveres new principles assets. On the basis of a questionnaire prepared, a survey was conducted of the local population where we find a close relation which exists between the plant species described in the said region and the different types of diseases affecting the human being; six plants have been chosen to evaluate the antifungal activity of essential oils against the bacterial strain (Staphylococcus aureus, and on two yeasts: Candida albicans and Candida glabrata, which are the most frequently identified in clinical specimens, a mold: Aspergillus niger, and 5 dermatophytes: Microsporum canis, Microsporum gypseum, Trichophyton rubrum, Trichophyton mentagrophytes, and Epidermophyton floccosum.Methods: The extraction of essential oils was carried out by steam distillation in a clevenger-type apparatus. The antimicrobial activity was determined by the disc diffusion method. The chromatographic analysis of HE five plants was performed with a gas chromatograph (GC) type Hewlett-Packard (6890) coupled to a mass spectrometer (HP5973).Results:  The results  show  that  the essential oil of Teucrium capitatum L. has a large antimicrobial activity vis-a-vis other plants. Therefore, an identification of their chemical composition is necessary to identify the active molecules based on the analysis GC-only and coupled to mass spectrometry (GC-MS). The essential oil of the plant of T. capitatum L. of the region of El Hajeb (Morocco) was extracted to the study its performance, its chemical composition, and its property antibacterial and antifungal. The average content in essential oil of the leaves of this species is approximately2% (wt.) of the total weight of dry matter. The chromatographic analysis showed the profiles, and the rates of the different components compared to the available standards, as function of the retention time by ascending order, four compounds were identified by GC and GC/MS as the main compounds of this oil: Endo-borneol (33%), naphthalene, 1, 2, 3, 5, 6, 8a-hexahydro-4,7-dimethyl-1-(1-methyl ethyl)-, (1s-cis)-(19.63%), bronyle acetate (15.56%), alpha-terpineol (11.96%), bicyclo[3.1.0]hexan-3-ol, 4-methyl-1-(1-methylethyl)-(10.94%) among other 21 compounds.Conclusion: This study allows, once again, the development of the exploitation of the essential oils in the areas, pharmaceutical and medicines and works the doors of the exploitation of these plants in the pharmaceutical market. Keywords: Teucrium capitatum L., Ethnobotanical study, Antifungal activity, Chromatography.

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