scholarly journals Optimization of Taxol Extraction Process Using Response Surface Methodology and Investigation of Temporal and Spatial Distribution of Taxol in Taxus mairei

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5485
Author(s):  
Lingyu Li ◽  
Yiming Chen ◽  
Yingli Ma ◽  
Zhong Wang ◽  
Tao Wang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Spatial Distribution ◽  
Extraction Process ◽  
Ultrasonic Extraction ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Temporal And Spatial Distribution ◽  
Taxus Mairei ◽  
Temporal And Spatial ◽  
Solid Liquid

Taxus mairei is an important source for industrial extraction of taxol in China. However, the standard and steps of extraction are currently not uniform, which seriously affects the taxol yield. In the present study, the influence of four factors (methanol concentration, solid-liquid ratio, ultrasonic extraction temperature, and ultrasonic extraction time) on the taxol yield was successively explored in T. mairei. A response surface methodology (RSM) was used to optimize the extraction process based on the single-factor experiments above. The optimal conditions were as follows: methanol concentration was 90%, solid-liquid ratio was 1:15 (g/mL), ultrasonic extraction temperature was 40 °C and ultrasonic extraction time was 60 min. Moreover, the twigs and needles from T. mairei with different tree ages were treated by the optimum extraction process, which further revealed temporal and spatial distribution of taxol in the reproducible tissues. Interestingly, the taxol content was relatively higher in needles of T. ‘Jinxishan’ (a cultivar from T. mairei with yellow aril, FY), but was less in FY twigs. The accumulation of taxol in twigs and leaves of females (with red aril, FR) was significantly higher than that of males (M); however, the content showed a decreasing trend with the increasing tree ages. Therefore, it is suitable to increase the proportion of female trees especially the FY leaves as raw materials for the industrial production of taxol from T. mairei, and the tree ages should be better controlled at 3–7 years.

Ultrasonic Extraction Chuanxiong Polysaccharide of Influencing Factors

2012 ◽  
Vol 550-553 ◽  
pp. 1071-1075
Author(s):  
Yong Guang Bi ◽  
Chun Chun Liu
Keyword(s):  
Influencing Factors ◽  
Extraction Process ◽  
Extraction Rate ◽  
Ultrasonic Extraction ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Ultrasonic Time ◽  
Solid Liquid

In order to study the the Chuanxiong polysaccharide ultrasonic extraction process, examine the ultrasonic time, ultrasonic power, solid-liquid ratio and extraction temperature on the Chuanxiong polysaccharide extract rate. The results show that the role of time at 30min Polysaccharide better, the extraction rate is 26.63%. when the ultrasonic power is 320W, Polysaccharide extraction rate is 26.15%.When the solid-liquid ratio is 1:20, polysaccharide extraction rate is 27.73%. When the extraction temperature is 60°C, the polysaccharide extraction rate is 27.73%.

scholarly journals Research on the Extraction Process in Aqueous Two-Phase System of Moringa Leaf Flavonoids

2021 ◽  
Vol 267 ◽  
pp. 02010
Author(s):  
Rui Wang ◽  
Xueqin Zhang ◽  
Qian Zhou
Keyword(s):  
Raw Materials ◽  
Extraction Process ◽  
Ultrasonic Extraction ◽  
Extraction Time ◽  
Phase System ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Solid Liquid

Moringa leaves were used as raw materials, ethanol and ammonium sulfate were used to construct a aqueous two-phase system to extract the flavonoids from Moringa leaves. The solid-liquid ratio, ethanol content, ultrasonic extraction time, and ultrasonic extraction temperature were selected as single factors, and the extraction process of flavonoids was optimized through single-factor experiments and orthogonal experiments. The optimal process was: 1:110 of solid-liquid ratio, 45 % of ethanol concentration, 15 minutes of ultrasonic extraction time, 70 °C of ultrasonic extraction temperature. Under this condition, the extraction rate of Moringa leaf flavonoids reached 8.37 %.

Optimization of Extraction Process for Carotenoids from Tamarillo Fruits by Response Surface Methodology

2013 ◽  
Vol 781-784 ◽  
pp. 1749-1754 ◽  
Author(s):  
Qing Song Yang ◽  
Yan Zhao
Keyword(s):  
Response Surface Methodology ◽  
Theoretical Prediction ◽  
Response Surface ◽  
Extraction Process ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Health Food ◽  
Liquid Ratio ◽  
Solid Liquid

Using Box-Behnken center composite design of three variables with three levels, the optimal extraction process for carotenoids from tamarillo fruits were determined by response surface methodology. The optimal extraction process was obtained as followed: solid-liquid ratio of 1:19.02(g/mL), extraction temperature of 44°C, and extraction time of 33.68 min. Under these conditions, the extraction of carotenoids content was 5.755[mg/100, similar to the theoretical prediction of 5.885[mg/100. This study confirms the efficiency of RSM as a simple and effective method and the great potential of tamarillo fruits as a health food and a resource of carotenoids.

scholarly journals The Effect of Operating Conditions on Curcumin Extracted from Turmeric by Hydrothermal Extraction

2019 ◽  
Vol 125 ◽  
pp. 19001
Author(s):  
Mohamad Endy Yulianto ◽  
Rizka Amalia ◽  
Vita Paramita ◽  
Indah Hartati ◽  
Nissa Ayu Maulinda ◽  
...  
Keyword(s):  
High Efficiency ◽  
Economic Value ◽  
Bioactive Compound ◽  
Extraction Process ◽  
Operating Conditions ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
High Concentration ◽  
Solid Liquid ◽  
Hydrothermal Extraction

Turmeric has a bioactive compound namely curcuminoid. It has many pharmacology effects such as anticancer, antidiabetic, antioxidant, hypolipidemic, anti-inflammatory, antimicrobial, antifertility, anti-venom, anti-coagulant, anti-HIV hepatoprotective, nephroprotective, and anticoagulant properties. To increase the economic value of turmeric, it is necessary to develop a hydrothermal extraction process of turmeric’s active compound. The advantages of hydrothermal extraction were inexpensive, abundant availability, high purity, non-toxic, and easy to handle. This research aims to study the effect of operating conditions : temperature (130-150oC), time (10-40 minutes) and solid:liquid ratio (1:10 and 1:12) on the bioactive compounds of turmeric extracted from hydrothermal extraction process. Generally, high extraction yield was obtained at higher extraction temperature (140 and 150oC). Under these conditions, with a lower solid : liquid ratio (1:10), high concentration of curcumin is produced. Further, a higher solid : liquid ratio will likely produce the opposite result, except when it operates at low extraction temperature. The high temperature of the pressurized liquid water can reduce the viscosity and surface tension of water so it will increase the diffusion rates and absorption. The higher the solid:liquid ratio, the greater the different concentration between interior and exterior cell, which promote the high efficiency of diffusion process.

Study on the Extraction and Stability of Strawberry Red Pigment

2013 ◽  
Vol 781-784 ◽  
pp. 1886-1890
Author(s):  
Fei Long Sun ◽  
Zhang Peng Li ◽  
Ting Li ◽  
Yong Fan ◽  
Yan Chu ◽  
...  
Keyword(s):  
Extraction Process ◽  
Potassium Sorbate ◽  
Extraction Temperature ◽  
Red Pigment ◽  
Optimum Conditions ◽  
Liquid Ratio ◽  
Extensive Range ◽  
Development And Utilization ◽  
The Stability ◽  
Solid Liquid

Natural pigments have raised a growing interest due to their extensive range of colors, innocuous and beneficial health effects. In this paper, the extraction process and stability of strawberry red pigment were studied. An orthogonal test [L16(4)4] was applied to get the best extraction conditions. Results showed that the optimum conditions were that the extraction temperature was 65C, the solid-liquid ratio was 1:5 (g/mL), the ratio of 1.5mol/L hydrochloride to 97.5% ethanol was 3:17 (mL/mL), and the extraction time was 4.5 hours. The effects of heat treatment, oxidant, sugar and preservatives on the stability of strawberry red pigment were also discussed. The stability of strawberry red pigment was found to be affected by high temperature, hydrogen peroxide, and potassium sorbate. This study is expected to provide a basis for further research, development, and utilization of strawberry red pigment.

Application of Ultrasonic Technology to Tea Saponin Extraction

2013 ◽  
Vol 781-784 ◽  
pp. 1923-1927
Author(s):  
Yu Qiong Wu ◽  
Wei Dong Man
Keyword(s):  
Ethanol Concentration ◽  
Ultrasonic Extraction ◽  
Ultrasonic Frequency ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Tea Saponin ◽  
Ultrasonic Technology ◽  
Ultrasonic Time ◽  
Solid Liquid

Commercial crude tea saponin as raw material, the effect of ultrasonic technology on tea saponin extraction was investigated. Ethanol was selected as the suitable extraction solvent. The six single factors including ethanol concentration, solid-liquid ratio, ultrasonic time, ultrasonic power, ultrasonic frequency and ultrasonic extraction temperature were studied. The four factors of ethanol concentration, solid-liquid ratio, ultrasonic time, ultrasonic temperature were mainly studied by designing orthogonal experimental. The results show that tea saponin content increases from 48.57% to 90.21% under optimum conditions of ethanol concentration 90% (mL/mL), ultrasonic extraction temperature 40 °C, solid-liquid ratio 1:9, ultrasonic extraction time 40 min. The result indicates that the content of the tea saponin could be effectively improved through the ultrasonic technology.

scholarly journals OPTIMIZATION OF BREWING WASTE’S (TRUB) PHENOLIC COMPOUNDS EXTRACTION BY ULTRASOUND ASSISTED USING RESPONSE SURFACE METHODOLOGY

Química Nova ◽  
2020 ◽  
Author(s):  
Bianca Gandolpho ◽  
Aline Almeida ◽  
Gabriel Gandolpho ◽  
Daniele Freitas ◽  
Otávio Gasparini ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Operating Conditions ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Ultrasound Assisted ◽  
Solid Liquid

The brewing waste, also known as trub, is an abundant by-product of the brewing industry. Such material presents high levels of phenolic compounds, which promote antioxidant, antimicrobial and antifungal effects, turning the trub economically attractive. In this study, the trub’s phenolic compounds were extracted by ultrasound-assisted extraction technology. Such experiments were conducted according to a central composite rotatable design (CCRD), with the evaluated parameters being ethanol concentration, solid-liquid ratio and extraction temperature. Response surface methodology (RSM) described the effect of process’ variables through second order polynomial models, adjusted appropriately for such analysis, and optimized the operating conditions, aiming to obtain the maximum extraction of phenolic compounds through the proposed technique. The extraction’s optimal conditions for the evaluated variables were ethanol concentration of 58%, solid-liquid ratio of 1 g per 32 mL, and extraction temperature of 36 ºC, during a 30 minute process. Considering such experimental conditions, the total amount of phenolic compounds was equal to 7.23 mg of gallic acid g-1 trub, indicating that a great concentration of phenolic compounds can be extracted from this material through the proposed technique. Thus, this indicates that trub might be a promising by-product that can be used in different industrial fields.

Study on Extracting Technology of Vc from Momordica charantia

2013 ◽  
Vol 781-784 ◽  
pp. 606-609
Author(s):  
Chen Guang Jiang ◽  
Qing Jie Tang
Keyword(s):  
Extraction Yield ◽  
Orthogonal Test ◽  
Ultrasonic Extraction ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Technological Parameters ◽  
Test Analysis ◽  
Solid Liquid ◽  
Temperature Time

Balsam pear was used as the research object in experiment, the technological parameters were studied such as the way of crushing, leaching agent type and its concentration, solid-liquid ratio, ultrasonic extraction temperature, time, power's influence on the extraction yield of Vc, and the ultrasonic extraction time, temperature, ultrasonic power on the L9 (34) orthogonal test analysis. Results showed that bitter melon slices, and solid-liquid ratio is 1:7, citric acid as leaching agent and the concentration of 1%, ultrasonic power is 80 w, leaching temperature 30 °C, 40 min leaching time, leaching of balsam pear Vc the highest rate.

scholarly journals Optimization of DHEA Extraction from Sweet Potato Pomace by Ultrasonic-Microwave Synergistic Employing Response Surface Methodology

2019 ◽  
Vol 102 (2) ◽  
pp. 680-682
Author(s):  
Junjian Ran ◽  
Xinhong Liang ◽  
Hanmei Du ◽  
Junliang Sun
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Sweet Potato ◽  
Extraction Method ◽  
Extraction Efficiency ◽  
Extraction Yield ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Solid Liquid ◽  
Optimum Extraction

Abstract Background: A lot of sweet potato residues (SPR) were discarded and wasted. Objective: To make full use of the SPR. Methods: Ultrasonic microwave synergistic (UMS) extraction method was used to extractdehydroepiandrosterone (DHEA) in SPR. The extraction conditions were optimized by response surface methodology based on single factors. Results: The optimum extraction conditions were 1:25 (solid–liquid ratio), 300 W (microwave power), 30 min (extraction time), and 30°C (extraction temperature). The extraction yield of DHEA from SPR reached 117.25 μg/100 g. Conclusions: The advantage of UMS extractiontechnology is to make full use of the synergistic effect of ultrasound and microwave to improve extraction efficiency. Highlights: The technology provides an effective way to improve the DHEA extraction yield from the SPR in industrial production.

scholarly journals The Optimization of anti-tyrosinase activity in extraction of Persicaria pulchra (Bl.) Soják by using Response Surface Methodology (RSM)

2020 ◽  
Vol 3 (4) ◽  
pp. first
Author(s):  
Hà Cẩm Anh ◽  
Vũ Thị Ái Xuân ◽  
Lê Minh Tấn
Keyword(s):  
Mathematical Modeling ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Tyrosinase Activity ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Liquid Ratio ◽  
Solid Liquid

In these recent years, natural compound industry has been more and more attractive many interested. Although Persicaria pulchra (Bl.) Soják has been applied in Eastern medicine for long time, it has not researched deeply the chemical compounds and biological activities. In the experience, the presence of the polyphenol, flavonoid and alkaloid compounds with the value IC50 = 127,99 mg/mL proved that this plant owns potential anti-tyrosinase activity. To expand its using in the pharmaceutical and cosmetics industries, optimize the extraction conditions for tyrosinase inhibition from Persicaria pulchra (Bl.) Soják leaves using response surface methodology (RSM). The effect of the factors including ethanol concentration, temperature, solid/liquid ratio and extraction time on extraction of tyrosinase enzyme inhibitor from the Persicaria pulchra (Bl.) Soják leaves was investigated by experimental and Response Surface Methodology (RSM) methods. Results demonstrated that the mathematical modeling are compatible with experimental data and all four factors exhibit significant effect on tyrosinase inhibition activity of the extract (p<0,05). The optimal extraction conditions conjectured by the mathematical modeling as the content of solvent of 63% ethanol, the extraction temperature of 56.5 oC, the solid / liquid ratio 1: 8.18 g/mL, and the extraction time of 13.96 minutes. Under these conditions, the extract indicated that the best tyrosinase inhibition value of the extract for IC50 was 56,39 mg/mL, 2.5 times lower than the preliminary experiment value (127,99 mg/mL). Additionally, the experiment results of tyrosinase inhibition activity of optimal extraction showed that the results exported from mathematical modeling was equivalent with them.

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