scholarly journals The Optimization of anti-tyrosinase activity in extraction of Persicaria pulchra (Bl.) Soják by using Response Surface Methodology (RSM)

2020 ◽  
Vol 3 (4) ◽  
pp. first
Author(s):  
Hà Cẩm Anh ◽  
Vũ Thị Ái Xuân ◽  
Lê Minh Tấn
Keyword(s):  
Mathematical Modeling ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Tyrosinase Activity ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Liquid Ratio ◽  
Solid Liquid

In these recent years, natural compound industry has been more and more attractive many interested. Although Persicaria pulchra (Bl.) Soják has been applied in Eastern medicine for long time, it has not researched deeply the chemical compounds and biological activities. In the experience, the presence of the polyphenol, flavonoid and alkaloid compounds with the value IC50 = 127,99 mg/mL proved that this plant owns potential anti-tyrosinase activity. To expand its using in the pharmaceutical and cosmetics industries, optimize the extraction conditions for tyrosinase inhibition from Persicaria pulchra (Bl.) Soják leaves using response surface methodology (RSM). The effect of the factors including ethanol concentration, temperature, solid/liquid ratio and extraction time on extraction of tyrosinase enzyme inhibitor from the Persicaria pulchra (Bl.) Soják leaves was investigated by experimental and Response Surface Methodology (RSM) methods. Results demonstrated that the mathematical modeling are compatible with experimental data and all four factors exhibit significant effect on tyrosinase inhibition activity of the extract (p<0,05). The optimal extraction conditions conjectured by the mathematical modeling as the content of solvent of 63% ethanol, the extraction temperature of 56.5 oC, the solid / liquid ratio 1: 8.18 g/mL, and the extraction time of 13.96 minutes. Under these conditions, the extract indicated that the best tyrosinase inhibition value of the extract for IC50 was 56,39 mg/mL, 2.5 times lower than the preliminary experiment value (127,99 mg/mL). Additionally, the experiment results of tyrosinase inhibition activity of optimal extraction showed that the results exported from mathematical modeling was equivalent with them.

Optimization of Extraction Process for Carotenoids from Tamarillo Fruits by Response Surface Methodology

2013 ◽  
Vol 781-784 ◽  
pp. 1749-1754 ◽  
Author(s):  
Qing Song Yang ◽  
Yan Zhao
Keyword(s):  
Response Surface Methodology ◽  
Theoretical Prediction ◽  
Response Surface ◽  
Extraction Process ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Health Food ◽  
Liquid Ratio ◽  
Solid Liquid

Using Box-Behnken center composite design of three variables with three levels, the optimal extraction process for carotenoids from tamarillo fruits were determined by response surface methodology. The optimal extraction process was obtained as followed: solid-liquid ratio of 1:19.02(g/mL), extraction temperature of 44°C, and extraction time of 33.68 min. Under these conditions, the extraction of carotenoids content was 5.755[mg/100, similar to the theoretical prediction of 5.885[mg/100. This study confirms the efficiency of RSM as a simple and effective method and the great potential of tamarillo fruits as a health food and a resource of carotenoids.

Optimization of Ultrasound Extraction of Nitrite Nitrogen in Sediment by Response Surface Methodology

2014 ◽  
Vol 955-959 ◽  
pp. 1466-1470 ◽  
Author(s):  
Dan Liu ◽  
Fang Yu ◽  
Wei Tan ◽  
Gui Zhen Li ◽  
Min Yang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Optimum Condition ◽  
Response Surface ◽  
Process Condition ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Liquid Ratio ◽  
Ultrasound Extraction ◽  
Nitrite Nitrogen ◽  
Solid Liquid

Response surface methodology based on single factors was used to optimize the process condition for extraction of nitrite nitrogen in sediment . The results indicated that the extraction amount of nitrite nitrogen was 9.5μg /g under the optimum condition of extraction time of 44min, ultrasonic power of 280W, solid-liquid ratio of 1:19,which was closed to the predicated yield of 9.3μg/g. The process can be used for the extraction of nitrite nitrogen in sediments.

scholarly journals OPTIMIZATION OF BREWING WASTE’S (TRUB) PHENOLIC COMPOUNDS EXTRACTION BY ULTRASOUND ASSISTED USING RESPONSE SURFACE METHODOLOGY

Química Nova ◽  
2020 ◽  
Author(s):  
Bianca Gandolpho ◽  
Aline Almeida ◽  
Gabriel Gandolpho ◽  
Daniele Freitas ◽  
Otávio Gasparini ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Operating Conditions ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Ultrasound Assisted ◽  
Solid Liquid

The brewing waste, also known as trub, is an abundant by-product of the brewing industry. Such material presents high levels of phenolic compounds, which promote antioxidant, antimicrobial and antifungal effects, turning the trub economically attractive. In this study, the trub’s phenolic compounds were extracted by ultrasound-assisted extraction technology. Such experiments were conducted according to a central composite rotatable design (CCRD), with the evaluated parameters being ethanol concentration, solid-liquid ratio and extraction temperature. Response surface methodology (RSM) described the effect of process’ variables through second order polynomial models, adjusted appropriately for such analysis, and optimized the operating conditions, aiming to obtain the maximum extraction of phenolic compounds through the proposed technique. The extraction’s optimal conditions for the evaluated variables were ethanol concentration of 58%, solid-liquid ratio of 1 g per 32 mL, and extraction temperature of 36 ºC, during a 30 minute process. Considering such experimental conditions, the total amount of phenolic compounds was equal to 7.23 mg of gallic acid g-1 trub, indicating that a great concentration of phenolic compounds can be extracted from this material through the proposed technique. Thus, this indicates that trub might be a promising by-product that can be used in different industrial fields.

scholarly journals Optimization of DHEA Extraction from Sweet Potato Pomace by Ultrasonic-Microwave Synergistic Employing Response Surface Methodology

2019 ◽  
Vol 102 (2) ◽  
pp. 680-682
Author(s):  
Junjian Ran ◽  
Xinhong Liang ◽  
Hanmei Du ◽  
Junliang Sun
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Sweet Potato ◽  
Extraction Method ◽  
Extraction Efficiency ◽  
Extraction Yield ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Solid Liquid ◽  
Optimum Extraction

Abstract Background: A lot of sweet potato residues (SPR) were discarded and wasted. Objective: To make full use of the SPR. Methods: Ultrasonic microwave synergistic (UMS) extraction method was used to extractdehydroepiandrosterone (DHEA) in SPR. The extraction conditions were optimized by response surface methodology based on single factors. Results: The optimum extraction conditions were 1:25 (solid–liquid ratio), 300 W (microwave power), 30 min (extraction time), and 30°C (extraction temperature). The extraction yield of DHEA from SPR reached 117.25 μg/100 g. Conclusions: The advantage of UMS extractiontechnology is to make full use of the synergistic effect of ultrasound and microwave to improve extraction efficiency. Highlights: The technology provides an effective way to improve the DHEA extraction yield from the SPR in industrial production.

Optimization of Extraction of Tannins from Banana Peel Using Response Surface Methodology

2014 ◽  
Vol 678 ◽  
pp. 566-571
Author(s):  
Ting Ru Wu ◽  
Hong Ling Wang ◽  
Shi Wen Jiang ◽  
Dan Dan Liu ◽  
Fei Wei
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Banana Peel ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Optimized Conditions ◽  
Tannin Extraction

Response surface methodology (RSM) was employed to optimize the extraction conditions of tannins from banana peels. Based on the single-factor experiments, four independent variables including ethanol concentration, solid to liquid ratio, extraction time and extraction temperature were selected as affecting factors. According to central composite design (CCD) the four factor, five levels experiments were carried out with the tannin extraction yield as evaluation index. The results show that the optimized conditions are as follows: ethanol concentration of 75.44%, solid to liquid ratio of 1:35.34, extraction time of 7.94 h, extraction temperature of 62.85°C. Under the optimized conditions, the predicted tannin extraction yield reaches 58.55%, while the actual extraction yield is 57.42% with the relative error of 1.13%.

Optimization of Extraction Technology of Favonoids from Kandelia Candel Leaves by Using Response Surface Methodology

2013 ◽  
Vol 704 ◽  
pp. 332-337
Author(s):  
Yan Ping Ji ◽  
Cheng Ping Li ◽  
Xing Xu ◽  
Lan Tang ◽  
Qian Tong
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Kandelia Candel ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Box Behnken Design ◽  
Extraction Parameters ◽  
Solid Liquid

This work is to extract flavonoids using ethanol refluxing method from the Kandelia candel leaves which are rich in flavonoids. Ethanol concentration, solid-liquid ratio, temperature and extraction time on the extraction rate were investigated by the single factor experiments. Box-Behnken design (BBD) based on response surface methodology (RSM) was adopted to optimize the parameters. The results showed the best extracting rate was more than 7.74%, when the extraction parameters were ethanol concentration 60%, extraction time 0.5 h and temperature 80°C.

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

scholarly journals Optimization of Supercritical Carbon Dioxide Extraction of Quercus infectoria Oil

2015 ◽  
Vol 74 (7) ◽  
Author(s):  
Liza Md Salleh ◽  
Stashia ELeaness Rosland Abel ◽  
Gholamreza Zahedi ◽  
Russly Abd Rahman ◽  
Hasmida Mohd Nasir ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Goodness Of Fit ◽  
Maximum Yield ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Predicted Values ◽  
Artificial Neural Network Ann ◽  
Quercus Infectoria

This current study focuses on the modelling and optimization of supercritical fluid extraction of Quercus infectoria galls oil. In this case, response surface methodology (RSM) and artificial neural network (ANN) were applied for the modelling and prediction of extraction yield of galls oil. A 17-run Box-Behnken Design (BBD) was employed to statistically optimize the process parameters of SC-CO2 extraction of Quercus infectoria galls at a condition as follows: pressure (5000, 6000, 7000 Psi), temperature (40, 50, 60°C) and extraction time (30, 45, 60 min). The maximum yield of the extracted oil is1.12 % and the optimum conditions are at an extraction pressure of 5574 Psi; extraction temperature of 75°C and extraction time of 54 min. Under the optimal conditions, the experimental results agree with the predicted values obtained through analysis of variance (ANOVA). This indicates a successful response surface methodology and highly satisfactory goodness of fit of the model used. The analysis of experimental design for process optimization results demonstrates that temperature and extraction time are the main parameters that influence the oil extraction of Quercus infectoria.

scholarly journals Development of Phaleria macrocarpa (Scheff.) Boerl Fruits Using Response Surface Methodology On Phenolic, Flavonoid and Antioxidants Properties

2018 ◽  
Author(s):  
Khurul Ain Mohamed Mahzir ◽  
Siti Salwa Abdul Gani ◽  
Nor Fadzillah Mokhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Time ◽  
Feed Ratio ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Phaleria Macrocarpa

In this study, the optimal condition for the extraction of antioxidants from the fruit Buah Mahkota Dewa (Phaleria macrocarpa) was determined by using Response Surface Methodology (RSM). The optimization was applied using central composite design (CCD) to investigate the three independent variables, namely extraction temperature (oC), extraction time (minutes) and extraction solvent to-feed ratio (%v/v) on the responses of free radical scavenging activity (DPPH), ferric ion reducing power assay (FRAP), total phenolic content (TPC) and total flavonoid content (TFC).The optimal conditions for the antioxidants extraction were found to be extraction temperature (64oC), extraction time (66 minutes) and solvent to-feed ratio (75 %v/v) with the highest percentage yield of DPPH, FRAP, TPC and TFC were 86.85%, 7.47%, 292.86 mg/g and 3.22 mg/g respectively. Moreover, the data were subjected to response surface methodology (RSM) and the results showed that the polynomial equations for all models were significant, did not show lack of fit, and presented adjusted determination coefficients (R2) above 99%, proving the yield of phenolic, flavonoid and antioxidants activities obtained experimentally were close to the predicted values and the suitability of the model employed in RSM to optimize the extraction conditions. Hence, in this study, the fruit from P.macrocarpa could be considered to have the strong antioxidant ability and can be used in various cosmeceutical or medicinal applications.

scholarly journals Optimization of the extraction of natural phenolic antioxidants from the seeds of Tamarindus indica L. - an undervalued by product of food processing - using response surface methodology

2018 ◽  
Vol 62 (1) ◽  
pp. 67-74 ◽  
Author(s):  
Atreyi Sarkar ◽  
Uma Ghosh
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Time ◽  
Phenolic Antioxidants ◽  
Extraction Temperature ◽  
Tamarindus Indica ◽  
Solvent Concentration ◽  
Antioxidant Power ◽  
Wide Range ◽  
Ferric Reducing Antioxidant Power

The seeds of Tamarindus indica are known to possess a wide range of phenolic compounds with high antioxidant activity as measured by the ferric reducing antioxidant power (FRAP). In the present study, the optimum conditions for the extraction of crude phenolic antioxidants from Tamarind seed were determined using response surface methodology (RSM). A central composite design (CCD) was used to investigate the effects of four independent variables, namely concentration of extractable solids in solvent (g/ml; X1), extraction time (h; X2), extraction temperature (°C; X3) and solvent concentration (%, v/v; X4) on the responses of total polyphenol content (TPC) and FRAP. The CCD consisted of 30 experimental runs. A second-order polynomial model was used for predicting the responses. Canonical analysis of the surface responses revealed that the predicted optimal conditions for the maximal yield of TPC and FRAP were concentration of extractable solids in solvent of 0.049 g/ml, extraction time of 3.24 h, extraction temperature of 45 °C and a solvent concentration of 50%. The experimental values in the optimised condition coincided with the predicted ones within a 95% confidence interval, hence indicating the suitability of the model and the success of RSM in optimizing the extraction parameters.

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