scholarly journals Bioactives from Bee Products and Accompanying Extracellular Vesicles as Novel Bioactive Components for Wound Healing

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3770
Author(s):  
Željka Peršurić ◽  
Sandra Kraljević Pavelić
Keyword(s):  
Mass Spectrometry ◽  
Wound Healing ◽  
Chemical Composition ◽  
Extracellular Vesicles ◽  
Royal Jelly ◽  
Geographical Origin ◽  
Bioactive Components ◽  
Animal Cells ◽  
Health Promoting ◽  
High Diversity

In recent years, interest has surged among researchers to determine compounds from bee products such as honey, royal jelly, propolis and bee pollen, which are beneficial to human health. Mass spectrometry techniques have shown that bee products contain a number of proven health-promoting compounds but also revealed rather high diversity in the chemical composition of bee products depending on several factors, such as for example botanical sources and geographical origin. In the present paper, we present recent scientific advances in the field of major bioactive compounds from bee products and corresponding regenerative properties. We also discuss extracellular vesicles from bee products as a potential novel bioactive nutraceutical component. Extracellular vesicles are cell-derived membranous structures that show promising potential in various therapeutic areas. It has been extensively reported that the use of vesicles, which are naturally formed in plant and animal cells, as delivery agents have many advantages. Whether the use of extracellular vesicles from bee products represents a new solution for wound healing remains still to be elucidated. However, promising results in specific applications of the bee products in wound healing and tissue regenerative properties of extracellular vesicles provide a good rationale to further explore this idea.

Characterization of the selected honeybee products based on omics techniques

2019 ◽  
Vol 88 (2) ◽  
pp. 129-132
Author(s):  
Eliza Matuszewska ◽  
Paweł Dereziński ◽  
Agnieszka Klupczyńska ◽  
Agata Światły-Błaszkiewicz ◽  
Szymon Plewa ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Dietary Supplements ◽  
Royal Jelly ◽  
Biologically Active ◽  
Human Organism ◽  
Pharmacological Properties ◽  
Bioactive Components ◽  
Honeybee Venom ◽  
Harmful Effects

to comprehensively characterize honeybee venom, royal jelly, propolis, and pollen, by applying advanced analytical and bioinformatics methodologies. Honeybee products (HBP) contain many bioactive components with both beneficial and harmful effects on the human organism. Nevertheless, the overall composition of the HBP remains not fully investigated. Thus, this research is focused on complementary proteomic and metabolomic characterization of biologically active compounds derived from HBP, regarding their toxicological and pharmacological properties. The objectives of the study will be achieved by the application of up to date mass spectrometry techniques. Due to increasing interest in using of HBP in medicine, this project will contribute to improving the safety of HBP‑derived dietary supplements and drugs.

scholarly journals Profiling of Flavonols in Seeds and Sprouts of Luffa cylindrica

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901
Author(s):  
Soon-Mi Shim ◽  
Tae-Sik Park
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Glucuronic Acid ◽  
Ultra Performance Liquid Chromatography ◽  
Agricultural Wastes ◽  
Liquid Chromatography Mass Spectrometry ◽  
Bioactive Components ◽  
Fresh Weight ◽  
Luffa Cylindrica ◽  
Health Promoting

Seeds of Luffa cylindrica (Luffa) have been considered as agricultural wastes. However, we hypothesized that the seeds and its sprouts may provide bioactive components that could provide health benefits for humans. The current study profiled the bioactive components in both seeds and sprouts of Luffa by using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS/MSn). Apigenin was identified as a major component in the seeds of Luffa, with a concentration of 2.89 mg from 1 g fresh weight. The bioactive components in the sprouts were myricetin, luteolin, and quercetin, with concentrations of 32.4, 12.5, and 32.5 μg from 1 g fresh weight, respectively. Apigenin metabolites, such as apigenin-glucuronic acid and apigenin-acetyl glucuronic acid, were also identified in the sprouts. This implied that apigenin in the seeds, when sprouting, was changed to other flavonols having a flavone backbone. Results from the current study suggest that both seeds and sprouts of Luffa could be a bio-resource for health-promoting food materials.

The Chemical Composition of Animal Cells and Their Intracellular Compartments Reconstructed from 3D Mass Spectrometry

2007 ◽  
Vol 46 (28) ◽  
pp. 5332-5335 ◽  
Author(s):  
Daniel Breitenstein ◽  
Christina E. Rommel ◽  
Rudolf Möllers ◽  
Joachim Wegener ◽  
Birgit Hagenhoff
Keyword(s):  
Mass Spectrometry ◽  
Chemical Composition ◽  
Animal Cells ◽  
Intracellular Compartments

scholarly journals ANALYSIS OF BIOACTIVE COMPONENTS FROM CHLOROFORM EXTRACT OF LYCOPERDON SP. (APIOPERDON)

2021 ◽  
pp. 1-3
Author(s):  
BHARATHNAIR ◽  
DELMY ABRAHAM ◽  
K. SARANYA ◽  
G. E. MALLIKARJUNASWAMY
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Bioactive Compounds ◽  
Chloroform Extract ◽  
Dichloroacetic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Bioactive Components ◽  
Chromatography Mass Spectrometry ◽  
Soxhlet Apparatus

Objective: The present work was done to find out various bioactive compounds present in the chloroform extract of Lycoperdon pyriforme using gas chromatography-mass spectrometry (GC-MS). Methods: L. pyriforme was collected cleaned to remove any dirt and oven dried at 60°C. The sample was then finely powdered and extracted with chloroform using Soxhlet apparatus. The chemical composition of chloroform extract was then analyzed using QP2010S-Shimadzu GC-MS instrument. Results: Chloroform extract resulted in the presence of 33 compounds with 1-Heneicosanol (11.17%) and E-15-Heptadecenal (11.08%) forming major compounds and 1-Tetradecanol (0.16%), Dichloroacetic acid, and decyl ester (0.15%) forming least. Conclusion: The results indicated the presence of a variety of compounds thus providing the information about various bioactive compounds present in Lycoperdon sp. and its further application in the field of pharmacology.

scholarly journals Characterization and Quantification of Major Flavonol Glycosides in Ramps (Allium tricoccum)

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3281 ◽  
Author(s):  
Wijdan M. Dabeek ◽  
Nik Kovinich ◽  
Callee Walsh ◽  
Melissa Ventura Marra
Keyword(s):  
Blood Pressure ◽  
Acetic Acid ◽  
Chemical Composition ◽  
Traditional Medicine ◽  
Appalachian Mountains ◽  
Dry Weight ◽  
Flavonol Glycosides ◽  
Bioactive Components ◽  
Health Promoting ◽  
Allium Tricoccum

The ramp (Allium tricoccum) is a traditional plant in the eastern Appalachian Mountains. Ramps have been used in traditional medicine for their health-promoting roles in lowering blood pressure and cholesterol. Information on the chemical composition of the potentially bioactive components in ramps is limited. Therefore, the aim of this work was to characterize and quantify major flavonols in ramps. Flavonoids were extracted in 50% methanol and 3% acetic acid. Characterization was conducted using UHPLC-PDA-MS and MS/MS, and quantification was performed using UHPLC-PDA detection. The major flavonol glycosides were kaempferol sophoroside glucuronide, quercetin sophoroside glucuronide, kaempferol rutinoside glucuronide, quercetin hexoside glucuronide, quercetin sophoroside, and kaempferol sophoroside. All conjugates were detected in leaves. Quercetin and kaempferol sophoroside glucuronide conjugates were detected in the stem, but no flavonol glycosides were detected in the bulb. The total amounts of the identified quercetin and kaempferol conjugates in whole ramps were 0.5972 ± 0.235 and 0.3792 ± 0.130 mg/g dry weight, respectively. Flavonol conjugates were concentrated in the leaves. To our knowledge, this work is the first to identify and quantify the major flavonol glycosides in ramps. Our findings suggest that specifically the leaves may harbor the potentially bioactive flavonols components of the plant.

scholarly journals Production of nonulosonic acids in the extracellular polymeric substances of “Candidatus Accumulibacter phosphatis”

2021 ◽  
Author(s):  
Sergio Tomás-Martínez ◽  
Hugo B.C. Kleikamp ◽  
Thomas R. Neu ◽  
Martin Pabst ◽  
David G. Weissbrodt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pathogenic Bacteria ◽  
Extracellular Polymeric Substances ◽  
Lectin Binding ◽  
Wide Distribution ◽  
Sialic Acids ◽  
Animal Cells ◽  
Microbial Life ◽  
Proteomic Data ◽  
Accumulibacter Phosphatis

Abstract Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as an important compound in the extracellular matrix of virtually all microbial life and in “Candidatus Accumulibacter phosphatis”, a well-studied polyphosphate-accumulating organism, in particular. Here, bioaggregates highly enriched with these bacteria (approx. 95% based on proteomic data) were used to study the production of NulOs in an enrichment of this microorganism. Fluorescence lectin-binding analysis, enzymatic quantification, and mass spectrometry were used to analyze the different NulOs present, showing a wide distribution and variety of these carbohydrates, such as sialic acids and bacterial NulOs, in the bioaggregates. Phylogenetic analysis confirmed the potential of “Ca. Accumulibacter” to produce different types of NulOs. Proteomic analysis showed the ability of “Ca. Accumulibacter” to reutilize and reincorporate these carbohydrates. This investigation points out the importance of diverse NulOs in non-pathogenic bacteria, which are normally overlooked. Sialic acids and other NulOs should be further investigated for their role in the ecology of “Ca. Accumulibacter” in particular, and biofilms in general. Key Points •“Ca. Accumulibacter” has the potential to produce a range of nonulosonic acids. •Mass spectrometry and lectin binding can reveal the presence and location of nonulosonic acids. •The role of nonulosonic acid in non-pathogenic bacteria needs to be studied in detail.

Nanoprojectile Secondary Ion Mass Spectrometry for Analysis of Extracellular Vesicles

2021 ◽  
Author(s):  
Dmitriy S. Verkhoturov ◽  
Bruno P. Crulhas ◽  
Michael J. Eller ◽  
Yong D. Han ◽  
Stanislav V. Verkhoturov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Extracellular Vesicles ◽  
Secondary Ion Mass Spectrometry ◽  
Ion Mass Spectrometry ◽  
Secondary Ion
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