Urine-Derived Stem Cells Secreting Small Extracellular Vesicles Loaded in Acellular Amniotic Membrane for Wound Healing Promotion in Aged Mice

2019 ◽  
Author(s):  
Yongjin Sun ◽  
Bi Chen ◽  
Juntao Zhang ◽  
Yunlong Yang ◽  
Haiyan Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Extracellular Vesicles ◽  
Amniotic Membrane ◽  
Aged Mice

Amniotic Membrane Loading Epidermal Stem Cells Accelerate Impaired Wound Healing in Diabetic Rats

2011 ◽  
Vol 214 ◽  
pp. 455-460
Author(s):  
Qing Ling Zhong ◽  
De Wu Liu ◽  
Fan Rong Liu
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Amniotic Membrane ◽  
Healing Rate ◽  
Diabetic Rats ◽  
Epidermal Stem Cells ◽  
Impaired Wound Healing ◽  
Wound Model ◽  
Wound Margin

Epidermal stem cells are essential in wound healing, but their amount and activity are decreased in diabetes which contributes to the impaired healing. This study evaluated the efficacy of amniotic membrane loading epidermal stem cells in the management of impaired wound in diabetes. Epidermal stem cells derived from SD rats were isolated, cultured, identified and labled with BrdU in vitro. The wound model of diabetic rats were established, then amniotic membrane loading labled BrdU epidermal stem cells were implanted to impaired wound. The results showed that wound healing rate in amniotic membrane loading epidermal stem cells was significantly higher than that in control groups 14 days after treatment. BrdU-positive cells in the wounds and newborn epidermis were visible. This indicated that amniotic membrane loading epidermal stem cells accelerates epidermal migration of wound margin and wound epithelialization in diabetic rats.

Bone marrow-derived mesenchymal stem cells and extracellular vesicles enriched collagen chitosan scaffold in skin wound healing (a rat model)

2020 ◽  
pp. 088532822096392
Author(s):  
Salma Abolgheit ◽  
Sally Abdelkader ◽  
Moustafa Aboushelib ◽  
Enas Omar ◽  
Radwa Mehanna
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Tensile Strength ◽  
Mesenchymal Stem Cells ◽  
Extracellular Vesicles ◽  
Skin Wound ◽  
Collagen Deposition ◽  
Skin Wound Healing ◽  
Chitosan Scaffolds

Background Over the past ten years, regenerative medicine has focused on the regeneration and the reconstruction of damaged, diseased, or lost tissues and organs. Skin, being the largest organ in the human body, had attained a good attraction in this field. Delayed wound healing is one of the most challenging clinical medicine complications. This study aimed to evaluate the collagen chitosan scaffold’s effect alone, or enriched with either bone marrow-derived mesenchymal stem cells (BM-MSCs) or their secreted extracellular vesicles (EVs) on the duration and quality of skin wound healing. Methods A full-thickness skin wound was induced on the back of 32 adult male Sprague-Dawley rats. The wounds were either covered with collagen chitosan scaffolds alone, scaffolds enriched with stem cells, or extracellular vesicles. Unprotected wounds were used as control. Healing duration, collagen deposition and alignment, CD 68+ macrophage count, and functional tensile strength of healed skin were assessed (α = 0.05, n = 8). Results The rate of skin healing was significantly accelerated in all treated groups compared to the control. Immuno-histochemical assessment of CD68+ macrophages showed enhanced macrophages count, in addition to higher collagen deposition and better collagen alignment in EVs and BM-MSCs treated groups compared to the control group. Higher tensile strength values reflected the better collagen deposition and alignment for these groups. EVs showed higher amounts of collagen deposition and better alignment compared to MSCs treated group. Conclusion The collagen chitosan scaffolds enriched with MSCs or their EVs improved wound healing and improved the quantity and remodeling of collagen with a better assignment to EVs.

Bone marrow derived mesenchymal stem cells from aged mice have reduced wound healing, angiogenesis, proliferation and anti-apoptosis capabilities

2012 ◽  
Vol 36 (8) ◽  
pp. 747-753 ◽  
Author(s):  
Mahmood Saba Choudhery ◽  
Mohsin Khan ◽  
Ruhma Mahmood ◽  
Azra Mehmood ◽  
Shaheen N. Khan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Aged Mice

Oleoyl-Chitosan-Based Nanofiber Mats Impregnated with Amniotic Membrane Derived Stem Cells for Accelerated Full-Thickness Excisional Wound Healing

2017 ◽  
Vol 3 (8) ◽  
pp. 1738-1749 ◽  
Author(s):  
Sayanti Datta ◽  
Arun Prabhu Rameshbabu ◽  
Kamakshi Bankoti ◽  
Priti Prasanna Maity ◽  
Dipankar Das ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Amniotic Membrane ◽  
Full Thickness ◽  
Excisional Wound ◽  
Nanofiber Mats

scholarly journals Mesenchymal Stem Cell-Derived Extracellular Vesicles for Corneal Wound Repair

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Hongyan Tao ◽  
Xiaoniao Chen ◽  
Hongmei Cao ◽  
Lingyue Zheng ◽  
Qian Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Stem Cell ◽  
Extracellular Vesicles ◽  
Wound Repair ◽  
Bioactive Molecules ◽  
Persistent Epithelial Defect ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Corneal Wound

With the immunoregulation potential, mesenchymal stem cells (MSCs) have been used for tissue regeneration by relieving inflammation in the injured tissues. When this repair process is interfered by immune disorders or pathological angiogenesis, the delays in corneal epithelial wound healing can lead to a persistent epithelial defect. Stem cell-derived extracellular vesicles (EVs), which carry abundant bioactive molecules from stem cells, have provided an alternative to regeneration therapy. In this study, we aimed to investigate if EVs from human placenta-derived MSCs (hP-MSCs) could ameliorate alkali injury of the cornea in the mouse model. 33.33 μg/μL EVs in 10 μL PBS were applied to the cornea. Repeat application three times, and 100 μg EVs (in 30 μL PBS) in total were administrated per day for two weeks. Our results revealed that EVs from hP-MSCs had preferable functions including enhancing proliferation and anti-inflammation and suppressing apoptosis of corneal epithelial cells. Furthermore, hP-MSC-derived EVs ameliorated mouse corneal wound healing by inhibiting angiogenesis and inflammation. Taken together, our current data suggested that hP-MSC-derived EVs have the beneficial effects of corneal wound healing, which provide alternative cell-free therapy with great practical value.

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