scholarly journals Development of Antimicrobial Laser-Induced Photodynamic Therapy Based on Ethylcellulose/Chitosan Nanocomposite with 5,10,15,20-Tetrakis(m-Hydroxyphenyl)porphyrin

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3551
Author(s):  
Mohamed S. Hasanin ◽  
Mohamed Abdelraof ◽  
Mohamed Fikry ◽  
Yasser M. Shaker ◽  
Ayman M. K. Sweed ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Microbial Growth ◽  
Positive Charge ◽  
Laser Light ◽  
Multidrug Resistant ◽  
Antimicrobial Photodynamic Therapy ◽  
Drug Resistant ◽  
Surface Loading ◽  
Chitosan Composite ◽  
Higher Doses

The development of new antimicrobial strategies that act more efficiently than traditional antibiotics is becoming a necessity to combat multidrug-resistant pathogens. Here we report the efficacy of laser-light-irradiated 5,10,15,20-tetrakis(m-hydroxyphenyl)porphyrin (mTHPP) loaded onto an ethylcellulose (EC)/chitosan (Chs) nanocomposite in eradicating multi-drug resistant Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans. Surface loading of the ethylcelllose/chitosan composite with mTHPP was carried out and the resulting nanocomposite was fully characterized. The results indicate that the prepared nanocomposite incorporates mTHPP inside, and that the composite acquired an overall positive charge. The incorporation of mTHPP into the nanocomposite enhanced the photo- and thermal stability. Different laser wavelengths (458; 476; 488; 515; 635 nm), powers (5–70 mW), and exposure times (15–45 min) were investigated in the antimicrobial photodynamic therapy (aPDT) experiments, with the best inhibition observed using 635 nm with the mTHPP EC/Chs nanocomposite for C. albicans (59 ± 0.21%), P. aeruginosa (71.7 ± 1.72%), and S. aureus (74.2 ± 1.26%) with illumination of only 15 min. Utilization of higher doses (70 mW) for longer periods achieved more eradication of microbial growth.

scholarly journals A nuclear targeted dual-photosensitizer for drug-resistant cancer therapy with NIR activated multiple ROS

2016 ◽  
Vol 7 (7) ◽  
pp. 4237-4244 ◽  
Author(s):  
Zhengze Yu ◽  
Wei Pan ◽  
Na Li ◽  
Bo Tang
Keyword(s):  
Reactive Oxygen Species ◽  
Drug Resistance ◽  
Photodynamic Therapy ◽  
Cancer Therapy ◽  
Laser Irradiation ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
Oxygen Species ◽  
Cancer Multiple ◽  
Nir Laser

A nuclear targeted dual-photosensitizer was developed for photodynamic therapy against multidrug resistant cancer. Multiple reactive oxygen species (ROS) could be generated in the nucleus to directly break DNA double strands with a single 980 nm NIR laser irradiation, regardless of drug resistance.

Antimicrobial Photodynamic Therapy on Drug-resistant Pseudomonas aeruginosa-induced Infection. An In Vivo Study†

2012 ◽  
Vol 88 (3) ◽  
pp. 590-595 ◽  
Author(s):  
Maria C. E. Hashimoto ◽  
Renato A. Prates ◽  
Ilka T. Kato ◽  
Silvia C. Núñez ◽  
Lília C. Courrol ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Photodynamic Therapy ◽  
In Vivo Study ◽  
Antimicrobial Photodynamic Therapy ◽  
Drug Resistant

scholarly journals Effective Photodynamic Inactivation of 26 Escherichia coli Strains with Different Antibiotic Susceptibility Profiles: A Planktonic and Biofilm Study

Antibiotics ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 98 ◽  
Author(s):  
Òscar Gulías ◽  
Giselle McKenzie ◽  
Miquel Bayó ◽  
Montserrat Agut ◽  
Santi Nonell
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Reference Strain ◽  
Red Light ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Antimicrobial Photodynamic Therapy ◽  
Strain Atcc

The emergence of multidrug-resistant bacteria is a growing problem and alternative therapies are being sought to effectively address this issue. The aim of this study is to assess a range of Escherichia coli strains’ susceptibility to Methylene Blue-mediated antimicrobial photodynamic therapy and determine if this is affected by their antibiotic-resistance profile. Two reference and twenty-four uropathogenic clinical E. coli strains were used in this study. All were tested in vitro for antimicrobial susceptibility against sixteen antibiotics. Strains underwent photodynamic treatments using the photosensitizer Methylene Blue with red light and tested in both planktonic and biofilm state. It was found that reference strain ATCC 25922 was susceptible to all tested antibiotics whereas reference strain ATCC 35218 showed resistance only to Ampicillin. With the exception of strains number 16 and 22, all of the isolated strains were multidrug-resistant according to the criteria established by the European Centre for Disease Prevention and Control and the Centre for Disease Control and Prevention, where acquired non-susceptibility to at least one agent in three or more antimicrobial categories is outlined. Photodynamic therapy induced more than 3 log10 colony-forming units’ reduction to all strains in planktonic state. Whereas when tested in biofilm state, two and a half times the original dose of methylene blue was necessary to cause a 3 log10 antimicrobial effect. There were statistically significant differences in susceptibility among the strains tested in both the planktonic and biofilm experiments. Nevertheless, antimicrobial photodynamic therapy could inactivate all multidrug-resistant strains in the planktonic and biofilm state.

scholarly journals Derivatives of Natural Chlorophylls as Agents for Antimicrobial Photodynamic Therapy

2021 ◽  
Vol 22 (12) ◽  
pp. 6392
Author(s):  
Nikita Suvorov ◽  
Viktor Pogorilyy ◽  
Ekaterina Diachkova ◽  
Yuri Vasil’ev ◽  
Andrey Mironov ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Heterocyclic Compounds ◽  
Resistant Bacteria ◽  
Antimicrobial Photodynamic Therapy ◽  
Drug Resistant ◽  
Oxygen Species ◽  
Major Research ◽  
Drug Resistant Bacteria ◽  
Research Challenge ◽  
Derivatives Of

The rapid growth of drug-resistant bacteria all over the world has given rise to a major research challenge, namely a search for alternative treatments to which bacteria will be unable to develop resistance. Photodynamic therapy is an approach of this kind. It involves the use of photosensitizers in combination with visible light at a certain wavelength to excite the former and generate reactive oxygen species. Various synthetic heterocyclic compounds are used as photosensitizers. Of these, derivatives of natural chlorophylls have a special place due to their properties. This review deals with the use of such compounds in antimicrobial PDT.

Photodynamic therapy with nanoparticles to combat microbial infection and resistance

Nanoscale ◽  
2020 ◽  
Vol 12 (41) ◽  
pp. 21034-21059
Author(s):  
Alina Bekmukhametova ◽  
Herleen Ruprai ◽  
James M. Hook ◽  
Damia Mawad ◽  
Jessica Houang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Antimicrobial Photodynamic Therapy ◽  
Drug Resistant ◽  
Cellular Interactions ◽  
Microbial Infection ◽  
Recent Developments ◽  
Safety Considerations ◽  
Nanoparticulate Systems

Recent developments of nanoparticles and nanoparticulate systems in antimicrobial photodynamic therapy, focusing on drug-resistant microbes, cellular interactions, and safety considerations.

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