scholarly journals Characterization of a Hg2+-Selective Fluorescent Probe Based on Rhodamine B and Its Imaging in Living Cells

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3385
Author(s):  
Wenting Zhang ◽  
Chunwei Yu ◽  
Mei Yang ◽  
Shaobai Wen ◽  
Jun Zhang
Keyword(s):  
Rhodamine B ◽  
Organic Molecule ◽  
Water Solution ◽  
Living Cells ◽  
Fluorescent Detection ◽  
Naked Eye ◽  
Good Biocompatibility ◽  
Low Cytotoxicity ◽  
Sensing Characteristics

A small organic molecule P was synthesized and characterized as a fluorometric and colorimetric dual-modal probe for Hg2+. The sensing characteristics of the proposed probe for Hg2+ were studied in detail. A fluorescent enhancing property at 583 nm (>30 fold) accompanied with a visible colorimetric change, from colorless to pink, was observed with the addition of Hg2+ to P in an ethanol-water solution (8:2, v/v, 20 mM HEPES, pH 7.0), which would be helpful to fabricate Hg2+-selective probes with “naked-eye” and fluorescent detection. Meanwhile, cellular experimental results demonstrated its low cytotoxicity and good biocompatibility, and the application of P for imaging of Hg2+ in living cells was satisfactory.

A smart sensor for rapid detection of lethal hydrazine in human blood and drinking water

2019 ◽  
Vol 43 (8) ◽  
pp. 3303-3308 ◽  
Author(s):  
Sima Paul ◽  
Rajesh Nandi ◽  
Kakali Ghoshal ◽  
Maitree Bhattacharyya ◽  
Dilip K. Maiti
Keyword(s):  
Drinking Water ◽  
Health Hazard ◽  
Industrial Effluent ◽  
Living Cells ◽  
Cell Permeability ◽  
Smart Sensor ◽  
Naked Eye ◽  
Good Cell ◽  
Low Cytotoxicity ◽  
Naked Eye Detection

A newly designed and synthesized probe showed good cell permeability, low cytotoxicity, fast fluorogenic recognition, and “naked-eye” detection of a lethal health hazard, hydrazine, even at concentrations significantly below the TLV levels present in living cells, drinking water and industrial effluent.

scholarly journals A new rhodamine fluorescent OFF-ON-OFF probe for selective sensing of Fe3+ and AcO- in aqueous media and living cells

2018 ◽  
Vol 238 ◽  
pp. 04002
Author(s):  
Qinglei Liu ◽  
Huimin Liu ◽  
Yanan Lei ◽  
Yan Gao ◽  
Bing Zhao
Keyword(s):  
Fluorescence Probe ◽  
Color Change ◽  
Aqueous Media ◽  
Living Cells ◽  
Eye Color ◽  
Naked Eye ◽  
Fluorescent Response ◽  
Good Biocompatibility ◽  
Anions And Cations ◽  
Displacement Approach

A new fluorescent chemosensor for Fe3+ was developed based on a rhodamine platform. L displayed highly selective and sensitive “OFF-ON” fluorescence response and naked-eye color change to Fe3+ in aqueous solution. The resulting L-Fe3+ complex was found to act as a selective“ ON-OFF” fluorescence probe for AcO- against common anions and cations with a Fe3+ displacement approach. The detection limits of L to Fe3+ and L-Fe3+ complex to AcO- were estimated to be 6.04×10-8 mol/L and 7.51×10-8 mol/L, respectively. The good biocompatibility of L enabled the investigation of fluorescent response for Fe3+ and AcO- in living Ana-1 cells by confocal microscope.

Rhodamine–benzothiazole conjugate as an efficient multimodal sensor for Hg2+ ions and its application to imaging in living cells

2018 ◽  
Vol 42 (14) ◽  
pp. 11665-11672 ◽  
Author(s):  
Perumal Sakthivel ◽  
Karuppannan Sekar ◽  
Gandhi Sivaraman ◽  
Subramanian Singaravadivel
Keyword(s):  
Rhodamine B ◽  
Color Change ◽  
Living Cells ◽  
Fluorescence Response ◽  
Eye Color ◽  
Naked Eye ◽  
Turn On ◽  
Rhodamine B Dye

A rhodamine B dye bearing a benzothiazole conjugate is designed and synthesized, it shows a highly selective and sensitive naked-eye color change and turn-on fluorescence response to Hg2+ ions.

Naked eye detection of glutathione in living cells using rhodamine B-functionalized gold nanoparticles coupled with FRET

2012 ◽  
Vol 92 (1) ◽  
pp. 778-782 ◽  
Author(s):  
Huai-Hong Cai ◽  
Hui Wang ◽  
Jinhui Wang ◽  
Wei Wei ◽  
Pei-Hui Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Rhodamine B ◽  
Living Cells ◽  
Eye Detection ◽  
Naked Eye ◽  
Functionalized Gold Nanoparticles ◽  
Naked Eye Detection

High Resolution Replication of Organoclay Surfaces

1982 ◽  
Vol 40 ◽  
pp. 576-577
Author(s):  
W. W. Barker ◽  
W. E. Rigsby ◽  
V. J. Hurst ◽  
W. J. Humphreys
Keyword(s):  
Clay Mineral ◽  
Organic Molecule ◽  
Organic Molecules ◽  
X Ray Diffraction ◽  
Xrd Pattern ◽  
X Ray ◽  
Naturally Occurring ◽  
Silicate Layers ◽  
Mineral Identification

Experimental clay mineral-organic molecule complexes long have been known and some of them have been extensively studied by X-ray diffraction methods. The organic molecules are adsorbed onto the surfaces of the clay minerals, or intercalated between the silicate layers. Natural organo-clays also are widely recognized but generally have not been well characterized. Widely used techniques for clay mineral identification involve treatment of the sample with H2 O2 or other oxidant to destroy any associated organics. This generally simplifies and intensifies the XRD pattern of the clay residue, but helps little with the characterization of the original organoclay. Adequate techniques for the direct observation of synthetic and naturally occurring organoclays are yet to be developed.

scholarly journals Real-Time Challenging of ERα Y537S Mutant Transcriptional Activity in Living Cells

Endocrines ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 54-64
Author(s):  
Manuela Cipolletti ◽  
Sara Pescatori ◽  
Filippo Acconcia
Keyword(s):  
Cell Line ◽  
Transcriptional Activity ◽  
Estrogen Receptor Α ◽  
Living Cells ◽  
Patient Treatment ◽  
Estrogen Responsive Element ◽  
Specific Proteins ◽  
Responsive Element ◽  
Mcf 7

Metastatic estrogen receptor α (ERα)-expressing breast cancer (BC) occurs after prolonged patient treatment with endocrine therapy (ET) (e.g., aromatase inhibitors—AI; 4OH-tamoxifen—4OH-Tam). Often these metastatic BCs express a mutated ERα variant (e.g., Y537S), which is transcriptionally hyperactive, sustains uncontrolled proliferation, and renders tumor cells insensitive to ET drugs. Therefore, new molecules blocking hyperactive Y537S ERα mutation transcriptional activity are requested. Here we generated an MCF-7 cell line expressing the Y537S ERα mutation stably expressing an estrogen-responsive element (ERE) promoter, which activity can be monitored in living cells. Characterization of this cell line shows both hyperactive basal transcriptional activity with respect to normal MCF-7 cells, which stably express the same ERE-based promoter and a decreased effect of selective ER downregulators (SERDs) in reducing Y537S ERα mutant transcriptional activity with respect to wild type ERα transcriptional activity. Kinetic profiles of Y537S ERα mutant-based transcription produced by both drugs inducing receptor degradation and siRNA-mediated depletion of specific proteins (e.g., FOXA1 and caveolin1) reveals biphasic dynamics of the inhibition of the receptor-regulated transcriptional effects. Overall, we report a new model where to study the behavior of the Y537S ERα mutant that can be used for the identification of new targets and pathways regulating the Y537S ERα transcriptional activity.

scholarly journals Silica Monolith for the Removal of Pollutants from Gas and Aqueous Phases

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1316
Author(s):  
Vanessa Miglio ◽  
Chiara Zaccone ◽  
Chiara Vittoni ◽  
Ilaria Braschi ◽  
Enrico Buscaroli ◽  
...  
Keyword(s):  
Rhodamine B ◽  
Water Solution ◽  
Synthesis Method ◽  
Textural Properties ◽  
Adsorption Properties ◽  
Water Soluble ◽  
Silica Monoliths ◽  
Physico Chemical ◽  
Gaseous Toluene ◽  
Mcm 41

This study focused on the application of mesoporous silica monoliths for the removal of organic pollutants. The physico-chemical textural and surface properties of the monoliths were investigated. The homogeneity of the textural properties along the entire length of the monoliths was assessed, as well as the reproducibility of the synthesis method. The adsorption properties of the monoliths for gaseous toluene, as a model of Volatile Organic Compounds (VOCs), were evaluated and compared to those of a reference meso-structured silica powder (MCM-41) of commercial origin. Silica monoliths adsorbed comparable amounts of toluene with respect to MCM-41, with better performances at low pressure. Finally, considering their potential application in water phase, the adsorption properties of monoliths toward Rhodamine B, selected as a model molecule of water soluble pollutants, were studied together with their stability in water. After 24 h of contact, the silica monoliths were able to adsorb up to the 70% of 1.5 × 10−2 mM Rhodamine B in water solution.

A naked-eye visible and turn-on fluorescence probe for Fe3+ and its bioimaging application in living cells

2016 ◽  
Vol 237 ◽  
pp. 501-508 ◽  
Author(s):  
Yaming Liu ◽  
Jinyu Zhang ◽  
Jiaxi Ru ◽  
Xiang Yao ◽  
Yang Yang ◽  
...  
Keyword(s):  
Fluorescence Probe ◽  
Living Cells ◽  
Naked Eye ◽  
Turn On
Sign in / Sign up

Export Citation Format

Share Document